Aspects pratiques concernant le diagnostic et le traitement des principales ectoparasitoses du chien et du chat

Conference given outside the academic context (1999)

Histopathological pattern and humoral immune response to a crude exo-antigen and purified keratinase of Microsporum canis in symptomatic and asymptomatic infected cats.

in Medical Mycology (1999), 37(1), 1-9

In order to understand better the mechanisms involved in the diverse clinical patterns in Microsporum canis-infected cats, the histopathological features were compared in symptomatic and asymptomatic ... [more ▼]

In order to understand better the mechanisms involved in the diverse clinical patterns in Microsporum canis-infected cats, the histopathological features were compared in symptomatic and asymptomatic infected cats. Additionally, the IgG immune response to a crude exo-antigen and purified keratinase of M. canis was studied by ELISA in cats of various clinical and mycological status. Acute and subacute perifolliculitis and folliculitis occurred more frequently in symptomatic than asymptomatic cats. The latter usually displayed signs of chronic inflammation and a marked infiltration of superficial dermis by mast cells, which would suggest that these animals present similarities to chronically dermatophytic humans or animals. When using a crude M. canis antigen, all infected cats were shown to have significantly higher levels of specific IgG when compared to culture negative and mechanical carrier-cats. In these non-infected animals, specific IgG was more frequently detected in adults than in young animals. No difference in anti-crude antigen specific IgG was observed between symptomatic and asymptomatic infected cats, indicating that the presence of IgG is probably unrelated to the clinical status of cats. Anti-keratinase specific IgG was only detected in one of the infected cats. [less ▲]

Isolation of Microsporum canis from asymptomatic cats can reflect passive mechanical carriage or true subclinical infection

Conference (1999)

Le virus de la diarrhee virale bovine et de la maladie des muqueuses (BVD/MD): diagnostic et comportement epidemiologique et biologique

Report (1999)

Purification and Characterization of a 315 Kda Keratinolytic Subtilisin-Like Serine Protease from Microsporum Canis and Evidence of Its Secretion in Naturally Infected Cats

in Medical Mycology (1998), 36(6), 395-404

A keratinolytic protease, secreted as the major component by a feline clinical isolate of Microsporum canis cultivated in a minimal medium containing cat keratin, was purified by affinity chromatography ... [more ▼]

A keratinolytic protease, secreted as the major component by a feline clinical isolate of Microsporum canis cultivated in a minimal medium containing cat keratin, was purified by affinity chromatography on bacitracin agarose and gel filtration. The apparent molecular mass of the enzyme was 31.5 kDa and the pI was 11.8. The enzyme was not glycosylated and its first 15 N-terminal amino acids showed numerous similarities with other fungal subtilisins. The optimum pH was around 9 while inactivation of the enzyme was reversible at pH 4, but not at pH 11. The enzyme was stable at 37 degrees C with an apparent optimum temperature around 55 degrees C. PMSF, soybean trypsin inhibitor (SBTI) and chymostatin strongly inhibited the proteinase. The highest affinity (Km of 0.37 mM) and physiological efficiency (k(cat)/Km) were obtained for the synthetic substrate N-Suc-Ala-Ala-Pro-Phe-p-nitroanilide. These results indicate that the keratinase belongs to the subtilisin-like serine protease family. Purified rabbit immunoglobulins G prepared against the keratinase and used in an immunohistochemical test allowed the detection of the keratinase produced by the fungus invading hair structures in naturally infected cats. The in vitro keratinolytic activity of the enzyme and its production in vivo suggest that it may contribute to pathogenicity. [less ▲]

Field Efficacy of Injectable Doramectin against Chorioptes Bovis in Naturally Infected Cattle

in Veterinary Record : Journal of the British Veterinary Association (1998), 142(1), 18-19

A single subcutaneous injection of doramectin at a dose rate of 200 micrograms/kg bodyweight was effective in controlling an infection of Chorioptes bovis mites in naturally infected cattle. From 14 days ... [more ▼]

A single subcutaneous injection of doramectin at a dose rate of 200 micrograms/kg bodyweight was effective in controlling an infection of Chorioptes bovis mites in naturally infected cattle. From 14 days after treatment, the geometric mean number of live mites was significantly lower (P < 0.001) in the doramectin-treated cattle than in the control group at each sampling until day 35. The percentage efficacy (treated versus controls) of doramectin against C bovis at day 35 was 99.9 per cent and the percentage reduction (day 35 versus day 0) in the treated animals was 99.3 per cent. At day 35, all seven controls were still positive for C bovis whereas five of the eight doramectin-treated animals were free of live mites. [less ▲]

Données récentes sur les trichophyties bovines

Scientific conference (1998)

Actualités sur les helminthoses digestives des carnivores domestiques

Conference given outside the academic context (1998)

Que signifie le portage asymptomatique de Microsporum canis chez le chat ?

Conference (1998)

Le virus de la diarrhée virale bovine et de la maladie des muqueuses (BVD/MD): diagnostic et comportement épidémiologique et biologique

Report (1998)