References of "Mignon, Bernard"
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See detailEntomopathogenic activity of Beauveria bassiana against Psoroptes cuniculi
Lekimme, M.; Mignon, Bernard ULg; Tombeux, S. et al

Poster (2003)

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See detailEntomopathogenic activity of Beauveria bassiana against Psoroptes cuniculi
Lekimme, M.; Mignon, Bernard ULg; Tombeux, S. et al

Poster (2003)

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See detailIn vivo detection of three Microsporum canis subtilisin-like serine protease mRNAs in infected guinea-pigs
Descamps, F.; Brouta, F.; Vermout, S. et al

Conference (2003)

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See detailEvaluation of the specific immune response against a recombinant keratinase of Microsporum canis
Vermout, S.; Brouta, F.; Descamps, F. et al

Conference (2003)

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See detailComptes-rendus du symposium « Les acarioses cutanées des animaux de compagnie en dermatologie vétérinaire »
Mignon, Bernard ULg

Book published by Presses de la Faculté de Médecine Vétérinaire de l’Université de Liège (2002)

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See detailSecreted metalloprotease gene family of Microsporum canis
Brouta, Frédéric; Descamps, Frédéric; Monod, Michel et al

in Infection and Immunity (2002), 70(10), 5676-5683

Keratinolytic proteases secreted by dermatophytes are likely to be virulence-related factors. Microsporum canis, the main agent of dermatophytosis in dogs and cats, causes a zoonosis that is frequently ... [more ▼]

Keratinolytic proteases secreted by dermatophytes are likely to be virulence-related factors. Microsporum canis, the main agent of dermatophytosis in dogs and cats, causes a zoonosis that is frequently reported. Using Aspergillus fumigatus metalloprotease genomic sequence (MEP) as a probe, three genes (MEP1, MEP2, and MEP3) were isolated from an M. canis genomic library. They presented a quite-high percentage of identity with both A. fumigatus MEP and Aspergillus oryzae neutral protease I genes. At the amino acid level, they all contained an HEXXH consensus sequence, confirming that these M. canis genes (MEP genes) encode a zinc-containing metalloprotease gene family. Furthermore, MEP3 was found to be the gene encoding a previously isolated M. canis 43.5-kDa keratinolytic metalloprotease, and was successfully expressed as an active recombinant enzyme in Pichia pastoris. Reverse transcriptase nested PCR performed on total RNA extracted from the hair of M. canis-infected guinea pigs showed that at least MEP2 and MEP3 are produced during the infection process. This is the first report describing the isolation of a gene family encoding potential virulence-related factors in dermatophytes. [less ▲]

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See detailIsolation of a Microsporum canis gene family encoding three subtilisin-like proteases expressed in vivo
Descamps, Frédéric; Brouta, Frédéric; Monod, Michel et al

in Journal of Investigative Dermatology (2002), 119(4), 830-835

Microsporum canis is the main agent of dermatophytosis in dogs and cats and is responsible for frequent zoonosis. The pathogenesis of the disease remains largely unknown, however. Among potential fungal ... [more ▼]

Microsporum canis is the main agent of dermatophytosis in dogs and cats and is responsible for frequent zoonosis. The pathogenesis of the disease remains largely unknown, however. Among potential fungal virulence factors are secreted keratinolytic proteases, whose molecular characterization would be an important step towards the understanding of dermatophytic infection pathogenesis. M. canis secretes a 31.5 kDa keratinolytic subtilisin-like protease as the major component in a culture medium containing cat keratin as the sole nitrogen source. Using a probe corresponding to a gene's internal fragment, which was obtained by polymerase chain reaction, the entire gene encoding this protease named SUB3 was cloned from a M. canis lambdaEMBL3 genomic library. Two closely related genes, termed SUB1 and SUB2, were also cloned from the library using as a probe the gene coding for Aspergillus fumigatus 33 kDa alkaline protease (ALP). Deduced amino acid sequence analysis revealed that SUB1, SUB2, and SUB3 are secreted proteases and show large regions of identity between themselves and with subtilisin-like proteases of other filamentous fungi. Interestingly, mRNA of SUB1, SUB2, and SUBS were detected by reverse transcriptase nested-polymerase chain reaction from hair of experimentally infected guinea pigs. These results show that SUB1, SUB2, and SUB3 encode a family of subtilisin-like proteases and strongly suggest that these proteases are produced by M. canis during the invasion of keratinized structures. This is the first report describing the isolation of a gene family encoding potential virulence-related factors in dermatophytes. [less ▲]

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See detailPossibilités de valorisation de nos recherches sur les dermatophytoses
Mignon, Bernard ULg

Scientific conference (2002)

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See detailIn vivo expression of a Microsporum canis 43.5 kDa metalloprotease in infected guinea pigs
Brouta, F.; Descamps, F.; Monod, M. et al

Poster (2002)

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See detailTranscription of the Microsporum canis 31.5 kDa keratinase gene in infected guinea-pigs
Descamps, F.; Brouta, F.; Vermout, S. et al

Poster (2002)

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See detailThe carriage of Echinococcus multilocularis by the red fox in southern Belgium
Losson, Bertrand ULg; Kervyn, T.; Mignon, Bernard ULg et al

Conference (2002)

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See detailLe portage asymptomatique de Microsporum canis chez le chat
Mignon, Bernard ULg; Brouta, F.; Descamps, F. et al

in Annales de Médecine Vétérinaire (2001), 145

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See detailNews on antifungal drugs registered in Belgium for the treatment of dermatophytosis in domestic carnivores.
Hamoir, J.; Goret, M.; Mignon, Bernard ULg et al

in Annales de Médecine Vétérinaire (2001), 145

This note gives a progress report on the various molecules registered in Belgium which can be used for the treatment of dermatophytosis in domestic carnivores. Its purpose is not to develop the ... [more ▼]

This note gives a progress report on the various molecules registered in Belgium which can be used for the treatment of dermatophytosis in domestic carnivores. Its purpose is not to develop the therapeutic protocols used in this type of diseases but rather to describe these compounds according to their pharmacological and toxicological characteristics as well as to define their status in the current legal framework. [less ▲]

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See detailPerspectives de vaccination anti-dermatophytique chez les carnivores domestiques
Descamps, F.; Brouta, F.; Losson, Bertrand ULg et al

in Annales de Médecine Vétérinaire (2001), 145

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See detailDonnées récentes sur la pathogenèse de l'infection à Microsporum canis chez les carnivores domestiques
Brouta, F.; Descamps, F.; Losson, Bertrand ULg et al

in Annales de Médecine Vétérinaire (2001), 145

Detailed reference viewed: 16 (1 ULg)