Showing results 121 to 140 of 247
 Conduite diagnostique des dermatoses prurigineuses des rongeurs et lagomorphes de compagnie : illustration à partir de cas cliniquesMignon, Bernard  Conference given outside the academic context (2003) Conduite diagnostique des dermatoses prurigineuses des rongeurs et lagomorphes de compagnie : illustration à partir de cas cliniquesMignon, Bernard Conference given outside the academic context (2003) Detailed reference viewed: 1 (0 ULg) A recombinant 31.5 kDa keratinase and a crude exo-antigen from Microsporum canis fail to protect against a homologous experimental infection in guinea pigs.; ; et al in Veterinary Dermatology (2003), 14(6), 305-312 A Microsporum canis recombinant 31.5 kDa keratinase and a M. canis crude exo-antigen were tested as vaccines in an experimental infection model in guinea pigs. Animals were vaccinated subcutaneously three ... [more ▼] A Microsporum canis recombinant 31.5 kDa keratinase and a M. canis crude exo-antigen were tested as vaccines in an experimental infection model in guinea pigs. Animals were vaccinated subcutaneously three times at two-week intervals with either the keratinase, the exo-antigen or the adjuvant alone. Cutaneous challenge was performed blindly. Both humoral and cellular-specific immune responses to M. canis antigens were evaluated every 14 days, while a blind evaluation of clinical lesion development and fungal persistency in skin were monitored weekly. Vaccination induced very high and significant (P < 0.01) antibody responses towards both antigens. High cell-mediated immune responses to both immunogens were also induced by vaccination. After challenge, however, scores reflecting the severity of dermatophytic lesions did not differ significantly between vaccinated and control groups at any time after challenge. These results suggest that, in the guinea pig, the induction of specific immune responses against the M. canis-secreted antigens used in this study are not protective against challenge exposure. [less ▲] Detailed reference viewed: 23 (3 ULg)Clinical, histopathological and immunological aspects of neosporosis in experimentally infected dogsLasri, Saadia ; ; et alConference (2003) Detailed reference viewed: 9 (0 ULg)Evaluation de la réponse immune spécifique contre une métalloprotéase kératinolytique de Microsporum canis recombinante chez le cobaye infecté expérimentalementMignon, Bernard ; ; et alConference (2003) Detailed reference viewed: 9 (3 ULg)Candida albicans cheilitis in guinea pig may be caused by commensal strains carried in the lower genital tractMignon, Bernard ; ; Losson, Bertrand in Veterinary Dermatology (2003), 14(5), 242 Detailed reference viewed: 105 (2 ULg)Echinococcus multilocularis in Wallonia (Southern Belgium): spatial distribution of carriage by the red fox (Vulpes vulpes) and preliminary results in the Musk rat (Ondatra zibethicus), a potentially important intermediate host; ; Mignon, Bernard et alPoster (2003) Detailed reference viewed: 6 (0 ULg)A recombinant 31.5 kDa keratinase and a crude exo-antigen from Microsporum canis fail to protect against a homologous experimental infection in guinea pigsMignon, Bernard ; ; et alConference (2003) Detailed reference viewed: 8 (4 ULg)Survey of cat and dog dermatophytosis in Europe. The ECMM working group report.; ; et al Conference (2003) Detailed reference viewed: 19 (2 ULg)Echinococcus multilocularis in Wallonia (Southern Belgium): spatial distribution of carriage by the red fox (Vulpes vulpes) and preliminary results in the Musk rat (Ondatra zibethicus), a potentially important intermediate host; ; Mignon, Bernard et alConference (2003) Detailed reference viewed: 15 (2 ULg)Hard ticks (Acarina, Ixodidae) found on domestic carnivores in Belgium: A survey conducted during three consecutive yearsLosson, Bertrand ; ; Maréchal, Françoise et alConference (2003) Detailed reference viewed: 40 (13 ULg)Epidémiologie de l'échinococcose alvéolaire en Région wallonne; Mignon, Bernard ; Losson, Bertrand Diverse speeche and writing (2003) Detailed reference viewed: 10 (2 ULg)Echinococcus multilocularis in Southern Belgium: spatial distribution of carriage by the red fox (Vulpes vulpes) and preliminary results in the musk rat (Ondatra zibethicus), a potentially important intermediate host; ; Mignon, Bernard et alPoster (2003) Detailed reference viewed: 12 (1 ULg)Entomopathogenic activity of Beauveria bassiana against Psoroptes cuniculi; Mignon, Bernard ; et alPoster (2003) Detailed reference viewed: 15 (6 ULg)Entomopathogenic activity of Beauveria bassiana against Psoroptes cuniculi; Mignon, Bernard ; et alPoster (2003) Detailed reference viewed: 9 (2 ULg)In vivo detection of three Microsporum canis subtilisin-like serine protease mRNAs in infected guinea-pigs; ; et al Conference (2003) Detailed reference viewed: 7 (2 ULg)Evaluation of the specific immune response against a recombinant keratinase of Microsporum canis; ; et al Conference (2003) Detailed reference viewed: 4 (1 ULg)Comptes-rendus du symposium « Les acarioses cutanées des animaux de compagnie en dermatologie vétérinaire »Mignon, Bernard Book published by Presses de la Faculté de Médecine Vétérinaire de l’Université de Liège (2002) Detailed reference viewed: 11 (4 ULg)Secreted metalloprotease gene family of Microsporum canis; ; et al in Infection and Immunity (2002), 70(10), 5676-5683 Keratinolytic proteases secreted by dermatophytes are likely to be virulence-related factors. Microsporum canis, the main agent of dermatophytosis in dogs and cats, causes a zoonosis that is frequently ... [more ▼] Keratinolytic proteases secreted by dermatophytes are likely to be virulence-related factors. Microsporum canis, the main agent of dermatophytosis in dogs and cats, causes a zoonosis that is frequently reported. Using Aspergillus fumigatus metalloprotease genomic sequence (MEP) as a probe, three genes (MEP1, MEP2, and MEP3) were isolated from an M. canis genomic library. They presented a quite-high percentage of identity with both A. fumigatus MEP and Aspergillus oryzae neutral protease I genes. At the amino acid level, they all contained an HEXXH consensus sequence, confirming that these M. canis genes (MEP genes) encode a zinc-containing metalloprotease gene family. Furthermore, MEP3 was found to be the gene encoding a previously isolated M. canis 43.5-kDa keratinolytic metalloprotease, and was successfully expressed as an active recombinant enzyme in Pichia pastoris. Reverse transcriptase nested PCR performed on total RNA extracted from the hair of M. canis-infected guinea pigs showed that at least MEP2 and MEP3 are produced during the infection process. This is the first report describing the isolation of a gene family encoding potential virulence-related factors in dermatophytes. [less ▲] Detailed reference viewed: 27 (1 ULg)Isolation of a Microsporum canis gene family encoding three subtilisin-like proteases expressed in vivo; ; et al in Journal of Investigative Dermatology (2002), 119(4), 830-835 Microsporum canis is the main agent of dermatophytosis in dogs and cats and is responsible for frequent zoonosis. The pathogenesis of the disease remains largely unknown, however. Among potential fungal ... [more ▼] Microsporum canis is the main agent of dermatophytosis in dogs and cats and is responsible for frequent zoonosis. The pathogenesis of the disease remains largely unknown, however. Among potential fungal virulence factors are secreted keratinolytic proteases, whose molecular characterization would be an important step towards the understanding of dermatophytic infection pathogenesis. M. canis secretes a 31.5 kDa keratinolytic subtilisin-like protease as the major component in a culture medium containing cat keratin as the sole nitrogen source. Using a probe corresponding to a gene's internal fragment, which was obtained by polymerase chain reaction, the entire gene encoding this protease named SUB3 was cloned from a M. canis lambdaEMBL3 genomic library. Two closely related genes, termed SUB1 and SUB2, were also cloned from the library using as a probe the gene coding for Aspergillus fumigatus 33 kDa alkaline protease (ALP). Deduced amino acid sequence analysis revealed that SUB1, SUB2, and SUB3 are secreted proteases and show large regions of identity between themselves and with subtilisin-like proteases of other filamentous fungi. Interestingly, mRNA of SUB1, SUB2, and SUBS were detected by reverse transcriptase nested-polymerase chain reaction from hair of experimentally infected guinea pigs. These results show that SUB1, SUB2, and SUB3 encode a family of subtilisin-like proteases and strongly suggest that these proteases are produced by M. canis during the invasion of keratinized structures. This is the first report describing the isolation of a gene family encoding potential virulence-related factors in dermatophytes. [less ▲] Detailed reference viewed: 27 (3 ULg) |
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