References of "Mignon, Bernard"
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See detailDermatophytosis: efficacy of commercial vaccines?
Mignon, Bernard ULg

in Proceedings of the 5th World Congress of Veterinary Dermatology (2004)

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See detailPrevalence of Toxocara canis eggs in faecal deposits from pet dogs in Liège, Belgium
Mignon, Bernard ULg; Vermout, S.; Moreaux, N. et al

Poster (2004)

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See detailEvaluation of immunogenicity and protective efficacy of a Microsporum canis metalloprotease subunit vaccine in guinea pigs.
Vermout, Sandy M; Brouta, Frederic D; Descamps, Frederic F et al

in FEMS Immunology & Medical Microbiology (2004), 40(1), 75-80

In order to identify protective immunogens against Microsporum canis infection, a purified recombinant keratinolytic metalloprotease (r-MEP3) was tested as a subunit vaccine in experimentally infected ... [more ▼]

In order to identify protective immunogens against Microsporum canis infection, a purified recombinant keratinolytic metalloprotease (r-MEP3) was tested as a subunit vaccine in experimentally infected guinea pigs. Both humoral and cellular specific immune responses developing towards r-MEP3 were evaluated, by enzyme-linked immunosorbent assay and by in vitro lymphocyte transformation tests respectively. Vaccination induced a strong antibody response, and a significant but transient lymphoproliferative response against the protein. However, the protocol failed to prevent fungal invasion or development of dermatophytic lesions. These results show that under the present experimental conditions, r-MEP3 specific antibodies are not protective against a challenge exposure. They also suggest that in the same model, the induction of cell-mediated immunity towards r-MEP3 is not sufficient, indicating the need for further research in the field of specific immune mechanisms involved in M. canis dermatophytosis. [less ▲]

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See detailExotic animal dermatology
Mignon, Bernard ULg; Losson, Bertrand ULg

Conference (2004)

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See detailImmunology of dermatophytosis
Mignon, Bernard ULg; Vermout, S.; Tabart, J. et al

Conference (2004)

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See detailIn vivo expression analysis of Microsporum canis secreted subtilisin-like serine proteases in feline dermatophytosis
Mignon, Bernard ULg; Descamps, F.; Brouta, F. et al

in Veterinary Dermatology (2004), 15(suppl 1), 17-18

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See detailPresentation of clinical cases
Mignon, Bernard ULg

Conference (2004)

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See detailHumoral and cellular immune response to a Microsporum canis recombinant keratinolytic metalloprotease (r-MEP3) in experimentally infected guinea pigs
Brouta, Frédéric; Descamps, Frédéric; Vermout, Sandy et al

in Medical Mycology (2003), 41(6), 495-501

In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a ... [more ▼]

In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a purified recombinant keratinolytic metalloprotease (r-MEP3) was evaluated in guinea pigs experimentally infected with M. canis. Humoral and cellular immune responses were assessed from day 0 to day 57 post-infection (PI), the former by enzyme-linked immunosorbent assay (ELISA) and the latter via a lymphocyte proliferation assay. Infected guinea pigs developed humoral and cellular responses to both M. canis exoantigen and r-MEP3, while no specific immune response to these antigens was observed in control animals. This is the first report on the development of both humoral and cell-mediated immune responses to a purified keratinase in M. canis dermatophytosis. [less ▲]

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See detailPrevalence of Echinococcus multilocularis in the red fox (Vulpes vulpes) in southern Belgium
Losson, Bertrand ULg; Kervyn, Thierry; Detry, Jacques et al

in Veterinary Parasitology (2003), 117(1-2), 23-28

Between June 1998 and February 2002,709 red foxes killed in Wallonia (south of Belgium) were available for parasitological examination of the gut. The identification of Echinococcus multilocularis was ... [more ▼]

Between June 1998 and February 2002,709 red foxes killed in Wallonia (south of Belgium) were available for parasitological examination of the gut. The identification of Echinococcus multilocularis was based on morphological data. E. multilocularis adults were observed in 20.2% of the animals. The analysis of data revealed marked differences between the geological areas of Wallonia; the highest prevalence (33%) was found in the Ardenne and the lowest (0%) on the Plateau de Herve. Host gender and the collection season had no effect on the prevalence. However, the latter was significantly higher in juveniles (<8 months of age). The geographical distribution of E. multilocularis in Belgium is much wider than originally thought. (C) 2003 Elsevier B.V. All rights reserved. [less ▲]

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See detailRecombinant expression and antigenic properties of a 31.5-kDa keratinolytic subtilisin-like serine protease from Microsporum canis
Descamps, Frédéric; Brouta, Frédéric; Vermout, Sandy et al

in FEMS Immunology & Medical Microbiology (2003), 38(1), 29-34

A secreted 31.5-kDa keratinolytic subtilase (SUB3; AJ431180) is thought to be a Microsporum canis virulence factor and represents a candidate for vaccination trials. In this study, the recombinant ... [more ▼]

A secreted 31.5-kDa keratinolytic subtilase (SUB3; AJ431180) is thought to be a Microsporum canis virulence factor and represents a candidate for vaccination trials. In this study, the recombinant keratinase (r-SUB3) was produced by the Pichia pastoris expression system and purified to homogeneity. Recombinant SUB3 displayed identical biochemical properties with the native protease. Experimentally cutaneously infected guinea pigs showed specific lymphoproliferative response towards r-SUB3, while no specific humoral immune response was induced except for one animal. The heterologous expression of SUB3 provides a valuable tool for addressing further investigations on the role of this keratinase in the specific cellular immune response and on its use in vaccination trials in the cat. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. [less ▲]

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See detailClinical, histopathological and immunological aspects of neosporosis in experimentally infected dogs
Lasri, Saadia ULg; Rettigner, C.; Onclin, K. et al

Conference (2003)

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See detailChoice of an adjuvant for vaccination trials
Vermout, Sandy; Denis, M.; Losson, Bertrand ULg et al

in Annales de Médecine Vétérinaire (2003), 147(6), 393-401

The development of new vaccines, containing protective antigens that are more and more well characterized, is hindered by the lack of adjuvants able both to amplify immune response and to control it ... [more ▼]

The development of new vaccines, containing protective antigens that are more and more well characterized, is hindered by the lack of adjuvants able both to amplify immune response and to control it qualitatively. A number of tumors and infectious diseases could be treated with adjuvant preparations that would adequately intensify and modulate specific immune response; in particular, the possibility to specifically induce a Th1 response seems to be of paramount importance for the prevention and for the cure of these diseases. Furthermore, these adjuvants must be as safe as possible, not only for commercial applications, but also in the respect of laboratory animals' welfare. This review describes the different adjuvants presently used in vaccinology, classifying them following their chemical nature and presenting for each category the knowledge concerning their activity and toxicity. [less ▲]

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