References of "Mignon, Bernard"
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See detailSecreted subtilisin gene family in Trichophyton rubrum
Jousson, O.; Lechenne, B.; Bontems, O. et al

in Gene (2004), 339

Secreted proteases constitute potential virulence factors of dermatophytes. A total of seven genes encoding putative serine proteases of the subtilisin family (SUB) were isolated in Trichophyton rubrum ... [more ▼]

Secreted proteases constitute potential virulence factors of dermatophytes. A total of seven genes encoding putative serine proteases of the subtilisin family (SUB) were isolated in Trichophyton rubrum. Based on sequence data and intron-exon structure, a phylogenetic analysis of subtilisins from T rubrum and other fungi revealed a presumed ancestral lineage comprising T rubrum SUB2 and Aspergillus SUBs. All other SUBs (SUB1, SUB3-7) are dermatophyte-specific and have apparently emerged more recently, through successive gene duplication events. We showed that two subtilisins, Sub3 and Sub4, were detected in culture supernatants of T rubrum grown in a medium containing soy protein as a sole nitrogen source. Both recombinant enzymes produced in Pichia pastoris are highly active on keratin azure suggesting that these proteases play an important role in invasion of keratinised tissues by the fungus. The set of deduced amino acid sequences of T rubrum SUB ORFs allowed the identification of orthologous Subs secreted by other dermatophyte species using proteolysis and mass spectrometry. (C) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailMultiplication of an ancestral gene encoding secreted fungalysin preceded species differentiation in the dermatophytes Trichophyton and Microsporum
Jousson, O.; Lechenne, B.; Bontems, O. et al

in Microbiology-Sgm (2004), 150(Part 2), 301-310

Dermatophytes are human and animal pathogenic fungi which cause cutaneous infections and grow exclusively in the stratum corneum, nails and hair. In a culture medium containing soy proteins as sole ... [more ▼]

Dermatophytes are human and animal pathogenic fungi which cause cutaneous infections and grow exclusively in the stratum corneum, nails and hair. In a culture medium containing soy proteins as sole nitrogen source a substantial proteolytic activity was secreted by Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. This proteolytic activity was 55-75% inhibited by o-phenanthroline, attesting that metalloproteases were secreted by all three species. Using a consensus probe constructed on previously characterized genes encoding metalloproteases (MEP) of the M36 fungalysin family in Aspergillus fumigatus, Aspergillus oryzae and M. canis, a five-member MEP family was isolated from genomic libraries of T rubrum, T mentagrophytes and M. canis. A phylogenetic analysis of genomic and protein sequences revealed a robust tree consisting of five main clades, each of them including a MEP sequence type from each dermatophyte species. Each MEP type was remarkably conserved across species (72-97% amino acid sequence identity). The tree topology clearly indicated that the multiplication of MEP genes in dermatophytes occurred prior to species divergence. In culture medium containing soy proteins as a sole nitrogen source secreted Meps accounted for 19-36% of total secreted protein extracts; characterization of protein bands by proteolysis and mass spectrometry revealed that the three dermatophyte species secreted two Meps (Mep3 and Mep4) encoded by orthologous genes. [less ▲]

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See detailRecent data on a current zoonosis: alveolar echinococcus due to Echinococcus multilocularis
Hanosset, Régis; Mignon, Bernard ULg; Losson, Bertrand ULg

in Annales de Médecine Vétérinaire (2004), 148(4), 153-167

E. multilocularis is a small tapeworm which uses different species of foxes and rodents as final and intermediate hosts respectively. In humans, infection with the metacestode stage can result in a ... [more ▼]

E. multilocularis is a small tapeworm which uses different species of foxes and rodents as final and intermediate hosts respectively. In humans, infection with the metacestode stage can result in a potentially lethal liver condition named alveolar echinococcosis (AE). Recent studies in central Europe have show that E. multilocularis has a wider geographic range than previously anticipated. By the end of the 1980s, endemic areas of E. multilocularis were know to exist in only four countries, but now the parasite is present in at least 14 European countries. This paper provide recent data on the biology of E. multilocularis, the epidemiology of AE and the different tools which are available to treat or prevent this disease which seems in apparent extension. [less ▲]

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See detailSevere fungal pneumonia in harbour porpoises stranded on belgian and french coastline in 2003
Jauniaux, Thierry ULg; Mignon, Bernard ULg; Coignoul, Freddy ULg

in Annual proceedings of the 18th annual conference of the European Cetacean Society (2004)

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See detailDermatophytoses - Mise au point
Mignon, Bernard ULg

Conference given outside the academic context (2004)

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See detailDermatophytosis: efficacy of commercial vaccines?
Mignon, Bernard ULg

in Proceedings of the 5th World Congress of Veterinary Dermatology (2004)

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See detailPrevalence of Toxocara canis eggs in faecal deposits from pet dogs in Liège, Belgium
Mignon, Bernard ULg; Vermout, S.; Moreaux, N. et al

Poster (2004)

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See detailEvaluation of immunogenicity and protective efficacy of a Microsporum canis metalloprotease subunit vaccine in guinea pigs.
Vermout, Sandy M; Brouta, Frederic D; Descamps, Frederic F et al

in FEMS Immunology & Medical Microbiology (2004), 40(1), 75-80

In order to identify protective immunogens against Microsporum canis infection, a purified recombinant keratinolytic metalloprotease (r-MEP3) was tested as a subunit vaccine in experimentally infected ... [more ▼]

In order to identify protective immunogens against Microsporum canis infection, a purified recombinant keratinolytic metalloprotease (r-MEP3) was tested as a subunit vaccine in experimentally infected guinea pigs. Both humoral and cellular specific immune responses developing towards r-MEP3 were evaluated, by enzyme-linked immunosorbent assay and by in vitro lymphocyte transformation tests respectively. Vaccination induced a strong antibody response, and a significant but transient lymphoproliferative response against the protein. However, the protocol failed to prevent fungal invasion or development of dermatophytic lesions. These results show that under the present experimental conditions, r-MEP3 specific antibodies are not protective against a challenge exposure. They also suggest that in the same model, the induction of cell-mediated immunity towards r-MEP3 is not sufficient, indicating the need for further research in the field of specific immune mechanisms involved in M. canis dermatophytosis. [less ▲]

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See detailExotic animal dermatology
Mignon, Bernard ULg; Losson, Bertrand ULg

Conference (2004)

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See detailImmunology of dermatophytosis
Mignon, Bernard ULg; Vermout, S.; Tabart, J. et al

Conference (2004)

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See detailIn vivo expression analysis of Microsporum canis secreted subtilisin-like serine proteases in feline dermatophytosis
Mignon, Bernard ULg; Descamps, F.; Brouta, F. et al

in Veterinary Dermatology (2004), 15(suppl 1), 17-18

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See detailPresentation of clinical cases
Mignon, Bernard ULg

Conference (2004)

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See detailEvaluation of immunogenicity and protective efficacy of a Microsporum canis metalloprotease subunit vaccine in guinea pigs
Vermout, S.; Brouta, F.; Descamps, F. et al

Conference (2004)

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See detailHumoral and cellular immune response to a Microsporum canis recombinant keratinolytic metalloprotease (r-MEP3) in experimentally infected guinea pigs
Brouta, Frédéric; Descamps, Frédéric; Vermout, Sandy et al

in Medical Mycology (2003), 41(6), 495-501

In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a ... [more ▼]

In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a purified recombinant keratinolytic metalloprotease (r-MEP3) was evaluated in guinea pigs experimentally infected with M. canis. Humoral and cellular immune responses were assessed from day 0 to day 57 post-infection (PI), the former by enzyme-linked immunosorbent assay (ELISA) and the latter via a lymphocyte proliferation assay. Infected guinea pigs developed humoral and cellular responses to both M. canis exoantigen and r-MEP3, while no specific immune response to these antigens was observed in control animals. This is the first report on the development of both humoral and cell-mediated immune responses to a purified keratinase in M. canis dermatophytosis. [less ▲]

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