References of "Merville, Marie-Paule"
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See detailAn in vivo model of varicella-zoster virus latent infection of dorsal root ganglia
Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg; Delrée, P. et al

in Journal of Neuroscience Research (1990), 26(1), 83-89

We describe here the first in vivo model of varicella-zoster virus (VZV) latent infection in the adult rat peripheral nervous system. Infected Mewo cells were injected subcutaneously along the spine of ... [more ▼]

We describe here the first in vivo model of varicella-zoster virus (VZV) latent infection in the adult rat peripheral nervous system. Infected Mewo cells were injected subcutaneously along the spine of healthy adult rats. No clinical sign of infection was observed even 9 months after inoculation. Humoral immune response to VZV was detected in all infected animals throughout the study (9 months). The presence of viral material in dissociated and cultured dorsal root ganglia (DRG) from inoculated animals was studied by immunoperoxidase and in situ hybridization. When DRGs from infected animals were plated in culture from 1 month and up to 9 months after inoculation, viral nucleic acids and proteins were detected in neurons. Furthermore, trypsinization and subcultivation of infected neurons in culture is needed to reactivate infectious virus at least in some of the neurons. This model provides a useful tool for studying 1) the molecular mechanisms leading to an in vivo latency, 2) the role of the immune system, in particular cellular immunity, on the establishment, maintenance, and reactivation of latency, 3) the neurotropism of mutant viruses, and 4) the effects of antiviral agents. [less ▲]

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See detailAcute and persistent varicella-zoster virus infection of human and murine neuroblastoma cell lines
Bourdon-Wouters, C.; Merville, Marie-Paule ULg; Sadzot-Delvaux, Catherine ULg et al

in Journal of Neuroscience Research (1990), 26(1), 90-97

Human and murine neuroblastoma cell lines were infected in vitro with varicella-zoster virus (VZV). Infected human neuroblastoma cells (IMR-32) supported the synthesis of abundant viral antigens as ... [more ▼]

Human and murine neuroblastoma cell lines were infected in vitro with varicella-zoster virus (VZV). Infected human neuroblastoma cells (IMR-32) supported the synthesis of abundant viral antigens as detected by indirect immunoperoxidase labeling using human serum rich in anti-VZV antibodies and did not survive the infection. In situ hybridization (ISH) with VZV-cloned probes revealed a strong hybridization signal in these infected cells. During cultivation, the virus was released in the culture medium, and viral polypeptides were revealed by Western blotting of infected cells, using either a monoclonal anti-gpI antibody or a rabbit antiserum. All these findings indicate that IMR-32 cells support a productive and lytic infection by VZV, whether infected by cell-free virus or by cocultivation with infected cells. Murine neuroblastoma cells (neuro-2A) survived VZV infection and did not produce any infectious virus. No VZV-specific proteins were detected in infected cells either by immunolabeling or by Western blotting. However, viral nucleic acids could be detected by ISH, indicating that mouse neuroblastoma cells displayed a nonproductive, nonlytic infection. Infected neuro-2A cells have been examined by ISH using probes corresponding to immediate early (IE) genes 4, 62, and 63 and late (L) gene 31 encoding gpII. A strong hybridization signal was detected when infected cells were probed with a fragment containing the IE genes 62 and 63. Lower levels of hybridization were detected with the other probes, corresponding to IE or L genes. These systems allow comparative molecular analysis of persistent and acute infection of nerve cells by VZV. [less ▲]

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See detailLatent infection in vivo by the varicella-zoster virus (VZV) in the rat nervous system
Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg; Delrée, P. et al

in Archives Internationales de Physiologie et de Biochimie (1989), 97

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See detailAntibodies to varicella-zoster virus modulate antigen distribution in cultured infected cells
Marc, Ph.; Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg et al

in Archives Internationales de Physiologie et de Biochimie (1989), 97

Detailed reference viewed: 8 (3 ULg)
See detailPersistent varicella-zoster virus infection in the nervous system: in vitro and in vivo models
Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg; Bourdon-Wouters, C. et al

in Acta Neurologica Belgica (1988), 88

Detailed reference viewed: 12 (0 ULg)
See detailIn vitro neuroblastoma cell infection by varicella-zoster virus
Bourdon-Wouters, C.; Merville, Marie-Paule ULg; Piette, Jacques ULg et al

Poster (1988)

Detailed reference viewed: 10 (0 ULg)
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See detailIn vitro and in vivo models of varicella-zoster virus persistence in the nervous system
Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg; Bourdon-Wouters, C. et al

in Journal of Cellular Biochemistry (1988), 12C

Detailed reference viewed: 14 (3 ULg)
See detailDetection of cytomegalovirus with labeled DNA probes
Bourdon-Wouters, C.; Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg et al

Poster (1987)

Detailed reference viewed: 12 (2 ULg)
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See detailMolecular cloning of varicella-zoster virus DNA and its detection in situ in infected nerve cells
Merville, Marie-Paule ULg; Sadzot-Delvaux, Catherine ULg; Delrée, P. et al

in Archives Internationales de Physiologie et de Biochimie (1987), 95(2), 31

Detailed reference viewed: 11 (2 ULg)
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See detailDetection and identification of the cytomegalovirus genome in infected cells with labeled DNA probes
Bourdon-Wouters, Christine; Merville, Marie-Paule ULg; Sadzot-Delvaux, Catherine ULg et al

in Archives Internationales de Physiologie et de Biochimie (1987)

Detailed reference viewed: 40 (18 ULg)
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See detailEffect of DNA photosensitization mediated by promazine derivatives on transcription in vitro
Decuyper, J.; Piette, Jacques ULg; Rentier, Bernard ULg et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1984), 92(2), 18

Detailed reference viewed: 14 (3 ULg)