References of "Melo de Sousa, Noelita"
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See detailFactors affecting plasma prolactin concentrations throughout gestation in high producing dairy cows.
Garcia-Ispierto, I.; Lopez-Gatius, F.; Almeria, S. et al

in Domestic Animal Endocrinology (2009), 36(2), 57-66

The aim of the present study was to investigate possible relationships between plasma concentrations of prolactin and the following factors throughout gestation in lactating dairy cows: photoperiod ... [more ▼]

The aim of the present study was to investigate possible relationships between plasma concentrations of prolactin and the following factors throughout gestation in lactating dairy cows: photoperiod, season, milk production, Neospora caninum-seropositivity, twin pregnancy, and plasma concentrations of pregnancy associated glycoproteins-1 (PAG-1), progesterone and cortisol. Pregnancy was diagnosed by trans-rectal ultrasonography on Day 40 post-insemination and by palpation per rectum on Days 90, 120, 150, 180 and 210 or until abortion in aborting cows. Blood samples were collected from each animal immediately before each pregnancy diagnosis. The study population was comprised of 73 non-aborting (54 Neospora-seropositive cows) and 20 aborting cows (all Neospora-seropositive) cows. In non-aborting cows, GLM repeated measures of analysis of variance revealed that lactation number and days in milk had no effect on plasma prolactin concentrations throughout gestation, whereas high plasma prolactin concentrations were significantly associated with high plasma levels of cortisol and PAG-1, with Neospora-seropositivity, positive photoperiod (increasing day length), twin pregnancy, and with low plasma progesterone concentrations. An interaction among plasma prolactin, plasma cortisol and milk production was observed in that plasma prolactin concentrations differed significantly throughout gestation and were highest in high-producing cows with high cortisol levels. In Neospora-seropositive non-aborting versus aborting cows, mean prolactin concentrations failed to differ on Days 40, 90, 120, 150 and 180 of pregnancy, whereas prolactin values were significantly lower (P=0.03) in aborting animals on Day 210 of pregnancy. Our results indicate that a positive photoperiod and Neospora-infection lead to increased plasma prolactin concentrations throughout gestation. Reduced prolactin concentrations detected in Neospora-seropositive aborting cows compared to non-aborting animals suggests a protective effect of prolactin in N. caninum infection. [less ▲]

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See detailPregnancy patterns during the early fetal period in high producing dairy cows treated with GnRH or progesterone.
Bech-Sabat, G.; Lopez-Gatius, F.; Garcia-Ispierto, I. et al

in Theriogenology (2009), 71

In order to explore pregnancy patterns in high producing dairy cows treated with GnRH or progesterone at pregnancy diagnosis (Days 28-34), two consecutive experiments were designed. In Experiment 1, cows ... [more ▼]

In order to explore pregnancy patterns in high producing dairy cows treated with GnRH or progesterone at pregnancy diagnosis (Days 28-34), two consecutive experiments were designed. In Experiment 1, cows bearing a single embryo were randomly assigned to a PRID (n=40; cows fitted with a progesterone releasing intra-vaginal device for 28 days), GnRH (n=40; cows receiving GnRH) or Control (n=26; untreated cows) group. PRID treatment led to a rise in plasma progesterone concentrations in the 7 days following the onset of treatment compared to the other two groups. In Experiment 2, in which we also examined twin pregnancies, animals were randomly assigned to PRID (n=312) or GnRH (n=294) treatment groups. Treatments were the same as described for Experiment 1. Logistic regression procedures revealed that in cows with a single corpus luteum, the probability of pregnancy loss between the first (Days 28-34) and second (Days 65-62) pregnancy diagnosis decreased by a factor of 0.51 in the PRID group compared to the GnRH group. However, in cows with two or more corpora lutea, PRID treatment increased the likelihood of pregnancy loss by a factor of three, compared to GnRH treatment. In cows carrying twins, the conceptus reduction rate was higher (P=0.02) for the GnRH (36%) than for the PRID (16.4%) group. Formation of a new corpus luteum was recorded in 17.7% of cows in the GnRH group. Our results indicate that compared to GnRH treatment, progesterone treatment given at pregnancy diagnosis in high producing dairy cows, reduced by a factor of 0.51 and increased by a factor of 3 the probability of pregnancy loss in cows with a single or with two or more corpora lutea, respectively, and reduced the conceptus reduction rate in cows carrying twins. The practical implications of our findings are that in herds with a high incidence of early fetal loss of a non-infectious nature, treatment at the time of pregnancy diagnosis with PRID in cows with one corpus luteum and with GnRH in cows with two or more corpora lutea should offer considerable benefits. [less ▲]

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See detailGamma interferon production correlates negatively with plasma levels of pregnancy-associated glycoprotein-1 (PAG-1) during gestation in dairy cows naturally infected with Neospora caninum
Lopez-Gatius, F.; Almeria, S.; Yániz, J. L. et al

in O’Leary, M.; Arnett, J. (Eds.) Pregnancy Protein Research (2009)

Gamma interferon (IFN-γ) production has been ascribed a role in protecting cows infected with Neospora caninum against abortion. The present study analyzes the interaction between IFN-γ production and ... [more ▼]

Gamma interferon (IFN-γ) production has been ascribed a role in protecting cows infected with Neospora caninum against abortion. The present study analyzes the interaction between IFN-γ production and levels of plasma pregnancy-associated glycoprotein-1 (PAG-1), as a marker of placental/fetal well-being, throughout gestation in naturally Neospora-infected dairy cows. Data were obtained from 88 pregnant cows from two herds: 62 seropositive and 26 seronegative for the parasite. Blood sample collection was performed on days 40, 90, 120, 150, 180 and 210 of gestation. Plasma was tested for antibodies against N. caninum, PAG-1 and IFN-γ. Twenty five (28.4%) pregnancies were recorded after AI using Holstein-Friesian semen (19 in seronegative and 6 in seropositive animals), and 63 (71.6%) after AI using Limousin semen (7 in seronegative and 56 in seropositive animals). Gamma interferon was detected in the plasma of 14 (22.6%) of the 62 Neospora-seropositive cows and could not be detected in any of the 26 seronegative animals. All 14 cows producing IFN-γ became pregnant by using Limousin semen. Our GLM repeated measures showed no effect of herd, lactation number, milk production at the time of pregnancy diagnosis and Neospora-seropositivity on plasma PAG-1 concentrations. Significant positive effects of both the day of gestation (P<0.0001) and interaction between day of gestation and breed of sire (P = 0.001) on PAG-1 values were registered. Cows carrying twins had higher (P = 0.002) PAG-1 concentrations throughout gestation than cows carrying singletons. Interactions between breed of sire and Neospora-seropositivity (P<0.0001), and between IFN-γ production and Neospora-seropositivity (P = 0.04) were also detected. Thus, Neospora-seronegative cows inseminated with Limousin and Neospora-seropositive cows with no IFN-γ production, exhibited higher PAG-1 concentrations throughout gestation than seropositive cows inseminated with Limousin semen and producing IFN-γ, respectively. Our data indicate that production of IFN-γ correlates negatively and, the production of antibodies against N. caninum is uncorrelated with plasma PAG-1 concentrations during gestation in Neospora-infected dairy cows. Our results also suggest that augmented Th1 cell-mediated immunity is related to a lower risk of abortion and therefore higher resistance to the parasite in cows inseminated with Limousin versus Holstein-Friesian semen. [less ▲]

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See detailCorrelation of five radioimmunoassay systems for measurement of bovine plasma pregnancy-associated glycoprotein concentrations at early pregnancy period.
Ayad, A.; Melo de Sousa, Noelita ULg; Sulon, Joseph ULg et al

in Research in Veterinary Science (2009), 86

The measurement of serum or plasma PAG concentrations is currently used as a specific method for pregnancy diagnosis in cattle. In this study, the correlation between five radioimmunoassay systems (RIA ... [more ▼]

The measurement of serum or plasma PAG concentrations is currently used as a specific method for pregnancy diagnosis in cattle. In this study, the correlation between five radioimmunoassay systems (RIA-497, RIA-706, RIA-780, RIA-809 and RIA-Pool) developed for measurement of PAG concentrations in ruminant species was investigated in plasma from pregnant Friesian Holstein females. Plasma PAG concentrations (ng/mL) measured by different RIA systems were significantly correlated between them ( 0.81; P<0.001). PAG concentrations increased significantly from Day 21 (n=27) to 30 (n=37) after AI by use of all PAG-RIA systems. From Day 30 to 80 after AI, lower PAG concentrations were observed when using the homologous system RIA-497. The addition of several proteinase inhibitors changed neither the non specific binding nor the B(0) binding to the tracer. Our results suggest that all tested PAG-RIA (RIA-497, RIA-706, RIA-780, RIA-809 and RIA-Pool) are highly correlated and can be useful to follow PAG concentrations in samples collected during the first trimester of gestation. [less ▲]

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See detailConcentrations of pregnancy-associated glycoproteins in Water buffaloes females (Bubalus bubalis) during pregnancy and postpartum periods
Barbato, O.; Melo de Sousa, Noelita ULg; Malfatti, A. et al

in O’Leary, M.; Arnett, J. (Eds.) Pregnancy Protein Research (2009)

The concentrations of pregnancy-associated glycoproteins (PAG) were determined in buffalo cows (Bubalus bubalis) using three different radioimmunoassay (RIA) systems (RIA-497, RIA-706, and RIA-708 ... [more ▼]

The concentrations of pregnancy-associated glycoproteins (PAG) were determined in buffalo cows (Bubalus bubalis) using three different radioimmunoassay (RIA) systems (RIA-497, RIA-706, and RIA-708). Samples (10 mL) were collected by jugular venipuncture from Week 0 until Week 28 of pregnancy (9 females), and from parturition until Week 10 postpartum (13 females). During pregnancy, PAG molecules were detectable at Week 6 using the three aforementioned RIA systems (3.9 ± 1.3 ng/mL, 9.7 ± 1.3 ng/mL, and 9.9 ± 0.7 ng/mL for RIA-497, RIA-706, and RIA-708, respectively). These concentrations increased gradually until Week 28, reaching values of 39.6 ± 4.0 ng/mL (RIA-497), 50.5 ± 11.9 ng/mL (RIA-706), and 68.2 ± 20.8 ng/mL (RIA-708). PAG concentrations determined by RIA-497, RIA-706, and RIA-708 were strongly correlated throughout the entire gestation period, RIA-708 giving the higher concentrations. At parturition, the mean concentrations ranged from 34.9 ± 4.0 (RIA-497) to 84.7 ± 10.6 ng/mL (RIA-708). Thereafter, the concentrations decreased steadily, reaching very low levels (< 1.0 ng/mL) at Week 8 postpartum. In conclusion, PAG concentrations measured by the above three RIA systems showed a profile similar to those previously described in bovine species, with higher concentrations being detected by RIA-706 and RIA-708. However, the patterns of PAG concentrations, studied using the three aforementioned PAG-RIA systems, differed around parturition, with very low levels being observed in the female buffaloes [less ▲]

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See detailIsolation of pregnancy-associated glycoproteins of the American bison (Bison bison) at first half of pregnancy
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

in General and Comparative Endocrinology (2008), 155(1), 164-175

This paper describes the successful purification and characterisation of pregnancy-associated glycoproteins (PAG) extracted from placenta (3-4 months) of American bisons (Amb). Chorionic AmbPAG proteins ... [more ▼]

This paper describes the successful purification and characterisation of pregnancy-associated glycoproteins (PAG) extracted from placenta (3-4 months) of American bisons (Amb). Chorionic AmbPAG proteins were purified from foetal cotyledonary tissues (CT) and liquid cotyledonary-carrying proteins (LCP) leaking from damaged cells. Our protocols successfully indicated the usefulness of AmbPAG protein identification, especially from LCP fraction. The AmbPAGs were extracted, precipitated and eluted during DEAE cellulose chromatography. The richest protein fractions were further chromatographed on VVA (Vicia villosa agglutinin affinity column), then characterised by mono- and bi-dimensional electrophoresis, Western blot and N-terminal amino acid (aa) sequence. After being transferred to PVDF membranes, three selected VVA-purified AmbPAG isoforms differing in molecular masses and isoelectric points (Ip 4-4.6) were selected for sequencing. One identified N-terminal 25 aa sequence of AmbPAG72 kDa CT form was identified as completely new (RGSNI_TSLPLQNVIDLFYVGNITIG). Two other AmbPAG proteins purified from different sources (74 kDa CT and 76 kDa LCP forms; RGSNLTIHPLRNIRDIFYVGNITIG) were identical or corresponded to N-terminus of various bovine PAGs (boPAG). The two AmbPAGs (74 kDa CT and 76 kDa LCP) revealed identical micro-sequence to boPAG7; and were similar mainly to bovine PAG4, -6, -15 and -17 precursors that were identified by full-length sequencing derived from cDNA cloning. The novel sequence of the AmbPAG (72 kDa CT) was related to some boPAG and various other ruminant PAG precursors (caprine and ovine). All three identified AmbPAG sequences were also relatively similar to mature forms of purified native boPAG56-75kDa proteins. This is the first report indicating aa sequences of native AmbPAG proteins purified from placenta (CT and LCP) of bison species. The N-terminal sequences of the AmbPAGs have been deposited in the EMBL-EBI database (UniProtKB; Accession Nos.: P84916, P84917 and P84918). (C) 2007 Elsevier Inc. All rights reserved. [less ▲]

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See detailRéussite à l'insémination en élevages ovins laitiers pyrénéens : facteurs de variantion liés aux conduites de troupeaux
Arranz, J. M.; Freret, S.; Fidelle, F. et al

in Proceedings de la 15e Journée sur les Rencontres de Recherche chez les Ruminants (2008)

En élevage ovin-lait, la réussite à l’insémination artificielle (IA) est un facteur clé pour la maîtrise de la reproduction et la mise en œuvre des schémas de sélection. Elle a été évaluée : 1) par dosage ... [more ▼]

En élevage ovin-lait, la réussite à l’insémination artificielle (IA) est un facteur clé pour la maîtrise de la reproduction et la mise en œuvre des schémas de sélection. Elle a été évaluée : 1) par dosage de la PAG plasmatique vingt huit jours après l’insémination ; 2) par l’analyse des résultats de mise bas (MB). La différence entre ces deux valeurs permet d’estimer le niveau de pertes embryonnaires et avortements. L’étude porte sur quatre-vingt cinq lots d’insémination (8841 brebis de races Manech / Latxa tête rousse et tête noire principalement) réalisés de mai à août 2007, dans trois centres d’insémination (CIA) transfrontaliers : Navarre, communauté autonome d’Euskadi et Pyrénées Atlantiques. Les données de contrôle laitier et des CIA ont été mobilisées pour étudier les facteurs de variation de la fertilité individuelle. Un deuxième volet de l’étude, à l’échelle des lots, traite des conduites d’élevage pendant la période de lutte. Il s’appuie sur des enquêtes (alimentation, chantiers d’IA, sanitaire). La fertilité à vingt huit jours s’élève à 61,3 % et celle à la MB à 55,2 %. Les pertes sont en moyenne de 6,2 %. Pour 2/3 des lots elles sont inférieures à 7 %. L’analyse des facteurs individuels, tant pour la fertilité à vingt huit jours qu’à la MB, confirme les effets classiques des facteurs de variation de la fertilité à l’IA : intervalle IA-MB précédente, âge des femelles, mode de reproduction de la campagne précédente, production laitière au moment de l’IA, qualité de la semence. Les niveaux de pertes sont plus élevés pour les jeunes ou lorsque l’intervalle est court. Au niveau des conduites d’élevage, on observe une interaction forte race*région associée à des périodes d’IA et des conduites alimentaires différentes, mais avec des effets peu ou pas significatifs de ces conduites. Les enquêtes sanitaires font apparaître des niveaux de risques et des pathologies comparables d’une région à l’autre et élevés. Pour les lots à pertes élevées, celles-ci ont pu être reliées à des problèmes pathologiques particuliers, mais non prévisibles. Une telle approche, assez complète et globale des conduites, aura permis de renouveler le conseil en élevage et d’élargir les capacités d’intervention des techniciens. [less ▲]

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See detailPlasma luteinizing hormone and progesterone concentrations in Azawak zebu cows submitted to different estrus synchronization protocols.
Zongo, M.; Pitala, W.; Sawadogo, L. et al

in Revue d'Elévage et de Médecine Vétérinaire des Pays Tropicaux (2008), 61(3-4), 221-227

The percentage of estrus induction and plasma LH concentrations were examined in 15 Azawak zebu cows submitted to two different estrus synchronization protocols. In the first treatment (T1, n = 9), the ... [more ▼]

The percentage of estrus induction and plasma LH concentrations were examined in 15 Azawak zebu cows submitted to two different estrus synchronization protocols. In the first treatment (T1, n = 9), the cows received a norgestomet ear-implant for 10 days associated with estradiol valerate, prostaglandin F2alpha (PGF2alpha) and pregnant mare serum gonadotropin (PMSG) injections. Treatment 2 (T2, n = 6) consisted in two PGF2alpha injections 11 days apart, followed by the administration of PMSG two days after the second prostaglandin injection. Blood samples were collected every three hours during five days after implant removal (T1) or PMSG injection (T2) for LH measurements. The proportion of induced estrus was higher in T2, but the difference was not significant. In general, the elapsed time until the estrus onset tended to be shorter in norgestomet-treated animals (35.9 ± 3.9 h) than in prostaglandin-treated ones (49.5 ± 5.8 h). Seven out of nine norgestomet-treated cows, and only two out of the six treated with PGF2alpha, presented a peak of LH. The mean interval from the end of treatments to LH peak tended to be longer in PGF2-treated females than in norgestomet-treated ones. One female presenting abnormally high LH concentrations after implant removal did not show a peak of LH during the observation period. [less ▲]

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See detailCorrelation between five radioimmunoassay systems for bovine pregnancy associated glycoprotein (PAG).
Ayad, A.; Melo de Sousa, Noelita ULg; Iguer-Ouada, M. et al

in Reproduction in Domestic Animals (2008), 43(Suppl 5), 90

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See detailNo effect of parity on concentrations of bovine pregnancy associated glycoprotein (PAG) measurement by radioimmunoassay.
Ayad, A.; Melo de Sousa, Noelita ULg; Iguer-Ouada, M. et al

in Reproduction in Domestic Animals (2008), 43(Suppl 5), 90

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See detailEffect of parity on pregnancy-associated glycoprotein (PAG) levels measurement by radioimmunoassay in bovine. Preliminary results.
Ayad, A.; Melo de Sousa, Noelita ULg; Iguer-Ouada, M. et al

in Proceedings of the 24nd Scientific Meeting of the European Embryo Transfer Association (2008)

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See detailDosage des pepsinogènes bovins A et C par RIA
Terroni, D.; Melo de Sousa, Noelita ULg; Michiels, J. A. et al

Poster (2008)

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See detailUnknown placental glycoprotein K-76 kDa [Fragment] from Bison bonasus placenta - Access number P85330
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailUnknown placental glycoprotein J2-45 kDa [Fragment] from Bison bonasus placenta - Access number P85329
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailUnknown placental glycoprotein J1-45 kDa [Fragment] from Bison bonasus placenta - P85328
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein I-50 kDa [Fragment] from Bison bonasus placenta - Access number P85327
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein H-60 kDa [Fragment] from Bison bonasus placenta - Access number P85326
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein G-71 kDa [Fragment] from Bison bonasus placenta - Access number P85325
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein F-50 kDa [Fragment] from Bison bonasus placenta - Access number P85324
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein E-55 kDa [Fragment] from Bison bonasus placenta - Access number P85323
Kiewisz, J.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

E-print/Working paper (2008)

N-terminal microsequence obtained after purification and characterization of placental proteins in Bison bonasus. Proteins were submitted to SwissProt databank.

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