References of "Melo de Sousa, Noelita"
     in
Bookmark and Share    
Full Text
See detailPrediction of stillbirth in Holstein-Friesian dairy cows by monotoring endocrine profiles in the periparturient period.
Szenci, O.; Bajcsy, A.Cs; Nagy, K. et al

in Proceedings of the 6th European Congress of Bovine Health and Management (ECBHM) (2011, September 07)

During the last decades, there is a declining trend in conception and calving rates in high yielding Holstein dairy cattle. At the same time, the gradual increase in stillbirth rates, especially in ... [more ▼]

During the last decades, there is a declining trend in conception and calving rates in high yielding Holstein dairy cattle. At the same time, the gradual increase in stillbirth rates, especially in Holstein heifers, has until recently received relatively little attention. Birth weight and gender of the calf, parity, age and breed of the dam, and season of calving are all factors associated with calving difficulties and stillbirths. Other causes of stillbirths not related to calving difficulties are, for example, herd size, infections (e.g. BVD), insufficient placenta development and/or function, metabolic disorders of the cow, and congenital malformations of the calf. The objective of the present study was to investigate the possible effect of hormonal disturbances on the incidence of stillbirth in dairy cows on a Holstein-Friesian dairy farm in Hungary. Dairy cows (n=173) were sampled three times during the periparturient period (at drying-off, 3 weeks prior to expected calving, and within 1 h after calving) in 2006 and two times (3 weeks prior to expected calving, and within 1 h after calving) in 2007. Dairy cows were grouped according to the incidence of stillbirth: Group 1 (control): n=165 and Group 2 (stillbirth): n=8. Hormone and pregnancy protein parameters measured by RIA were as follows: progesterone (P4), estradiol (E2), cortisol, triiodine-thyronin (T3), thyroxin (T4), insulin, insulin-like growth factor (IGF-1) and pregnancy associated glycoprotein (PAG). To determine, which of the hormones and PAG were the best predictors of the presence or absence of stillbirth at a given time (2 months and 3 weeks before calving and within 1 h after calving), a generalised linear model was used with binomial error distribution and logit link function (multivariate logistic regression). All analyses were carried out by R 2.7.2. Statistical Software. The significant level was set at P< 0.05 and an effect was considered a trend when P was between 0.05 and 0.10. The overall prevalence of stillbirth was 4.6 % (4.0% in 2006 and 5.4% in 2007). Interestingly, most calves (7 out of 8) with stillbirth were bulls, but this difference was not significant. Weight of the calves, body score condition of the cows and number of people needed to assist at calving also did not affect the presence or absence of stillbirth significantly, and there was no significant difference among the groups regarding the gestation length or parity either (P>0.100 in all cases). Regarding hormone and pregnancy protein parameters, no significant differences were found during the periparturient period (at 2 months and 3 weeks before expecting calving), however, there was a trend for lower IGF-1 values (P=0.079, OR=0.96) in stillbirth group compared to controls at dry off (2 months before expecting calving). Within one hour after calving significant differences were observed in case of P4 (P<0.001, OR= 3.06), and IGF-1 (P=0.021, OR= 0.94), resulting significantly higher values in P4 and lower values in IGF-1 in stillbirth cows compared to controls. The higher P4 values observed in stillbirth cows also resulted a higher P4/E2 ratio compared to controls (P=0.003, OR= 1.60). Changes in the IGF-1, P4 and P4/E2 ratio may be one of the reasons for stillbirth in Holstein-Friesian dairy cows however more examinations are needed to be confirmed. Other hormones (P4, cortisol, T3, T4, insulin, PAG) did not mirror dependency due to the incidence of stillbirth in dairy cows. [less ▲]

Detailed reference viewed: 124 (2 ULg)
Full Text
See detailDevelopment of a new ELISA for measurement of pregnancy-associated glycoprotein.
Delahaut, Ph; Melo de Sousa, Noelita ULg; Huet, A. C. et al

in Proceedings of the 6th European Congress of Bovine Health and Management (ECBHM) (2011, September 07)

In the present study we report the development of a new ELISA for PAG measurement (Ref. code E.G.7, CER, Maloie) in ruminant species. Purified boPAG67kDa protein was purified in our laboratory and used as ... [more ▼]

In the present study we report the development of a new ELISA for PAG measurement (Ref. code E.G.7, CER, Maloie) in ruminant species. Purified boPAG67kDa protein was purified in our laboratory and used as standard. Antiserum raised against caprine PAG 55kDa+62kDa (AS707) was used as capture antibody (1:40,000). Antiserum raised against PAG purified from buffalo placentas (AS859) was used as detection antibody (1:32,000 dilution). Avidin-HRP and TMB completed the ELISA system. Reproducibility was determined by calculating the intra- and inter-assay CV. Accuracy was determined by adding increasing concentrations of purified boPAG67kDa to bovine sera containing low PAG concentrations. Specificity was tested regarding 25 different compounds: carbohydrates, aspartic proteinases, hormones and major plasma proteins. Parallelism was assessed by serially diluting pregnant cow serum containing high PAG concentrations. Sera was collected from pregnant cows (n=4) during the whole duration of gestation. Samples were tested by both RIA and ELISA. Samples giving high PAG concentrations were serially diluted in order to fit with optical density (OD) of the ELISA standard curve. Intra-and inter-assay CV ranged from 5.5 to 9.1%. Accuracy ranged from 92.9 to 105.5%. No cross reaction was observed with almost all tested compounds excepting lactic acid at supra-physiological levels (1 mg/ml). Dilutions of bovine maternal sera were parallel to standard curve. Profiles obtained by ELISA in pregnant cows were very similar to those obtained by RIA. In conclusion, a new PAG-ELISA is available for measurement of PAG concentrations in cattle. This new test was validated in vitro and can be used for pregnancy diagnosis and follow-up of gestation in cattle. [less ▲]

Detailed reference viewed: 69 (3 ULg)
Full Text
See detailPregnancy diagnosis in cattle by using a new ELISA for pregnancy-associated glycoprotein: preliminary results.
Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg; Colemonts, Y. et al

in Proceedings of the 6th European Congress of Bovine Health and Management (ECBHM) (2011, September 07)

In the present study we report the use of a new PAG-ELISA for routine pregnancy diagnosis in cattle. A total of 2777 samples were collected from pregnant or non-pregnant cows and assayed by both classical ... [more ▼]

In the present study we report the use of a new PAG-ELISA for routine pregnancy diagnosis in cattle. A total of 2777 samples were collected from pregnant or non-pregnant cows and assayed by both classical PAG RIA-497 method and newly developed PAG-ELISA (Ref. code E.G.7, CER, Maloie). Pregnancy status was determined on the basis of RIA results. Samples were assumed to be issued from pregnant cows when PAG concentrations were higher than 0.8 ng/mL. PAG concentrations <0.8 ng/mL were considered negative (non-pregnant). PAG concentrations between 0.8 and 1.0 ng/mL were considered doubtful. With regard to ELISA, concentrations below 0.6 were considered negative. Concentrations between 0.6 and 0.8 ng/mL were considered doubtful and finally concentrations under 0.8 ng/mL were assumed as positive. Sensitivity(Se), specificity(Sp) and predictive values (PV) of pregnancy diagnosis were determined. A total of 143(5.2%), 137(4.9%) and 38(1.4%) samples gave doubtful results by RIA, ELISA or both systems, respectively. These samples (318/2777=11.5%) were not used for estimation of Se, Sp, PPV and NPV because clinical confirmation of pregnancy diagnosis could not be established by an additional method (rectal palpation, ultrasound or calving). Taking into account the remaining samples (2459), Se, Sp, PPV and NPV obtained by ELISA were respectively 98.9%, 89.9%, 98.7% and 96.4%. In conclusion, PAG-ELISA can be successfully used for pregnancy diagnosis in cattle. In the near future, further investigations are to be conducted in order to compare results obtained by ELISA test with those obtained by rectal palpation and ultrasound in field conditions. [less ▲]

Detailed reference viewed: 73 (5 ULg)
Full Text
Peer Reviewed
See detailClinical implications of induced twin reduction in dairy cattle
Andreu-Vazquez, C.; Garcia-Ispierto, I.; Lopez-Bejar, M. et al

in Theriogenology (2011), 76(3), 512-521

Embryo reduction may prevent the negative effects of twinning in dairy cattle; however, the technique may carry an additional risk of pregnancy loss. The aim of this study was to evaluate the effect on ... [more ▼]

Embryo reduction may prevent the negative effects of twinning in dairy cattle; however, the technique may carry an additional risk of pregnancy loss. The aim of this study was to evaluate the effect on pregnancy maintenance of embryo reduction by manual amnion rupture in unilateral and bilateral twin pregnant cows. A secondary objective was to examine the dynamics of endocrine factors following the treatment. On Day 35-41 of gestation 55 cows bearing two live twin embryos (28 bilateral, 27 unilateral) were randomly assigned to a twin reduction group (n = 27; cows fitted with a progesterone releasing intra-vaginal device for 21 days after manual amnion rupture) or control group (n = 28; untreated cows). Pregnancy loss before Day 90 was recorded in nine control and eleven twin reduction cows (32.1% vs 40.7%, respectively, p = 0.508). Logistic regression models indicated that laterality was the only variable significantly affecting pregnancy loss. The pregnancy loss risk was 8.7 times higher for unilateral than for bilateral twin pregnancies (59.3% vs 14.3%, respectively, P < 0.001) yet was similar in the unilateral control and unilateral twin reduction cows (62.3% vs 53.8%, respectively, P = 0.581). In contrast, four of 14 cows with bilateral twin pregnancies undergoing twin reduction lost their pregnancies while no losses were recorded in control cows with bilateral pregnancies (P = 0.049). A rise in plasma progesterone concentration was detected on the day following treatment in the twin reduction group and concentrations remained high within the first week of treatment. Plasma pregnancy-associated glycoprotein-1 (PAG-1) concentrations fell between Day 35-41 and Day 42-48, regardless of treatment. Our findings indicate that embryo reduction by manual amnion rupture did not carry an additional risk of pregnancy loss for unilateral twin pregnancies, whereas it increased the risk of pregnancy failure in bilateral twin pregnancies. However, benefits of preventing cows from delivering twins might also be considered when assessing the success of embryo reduction in bilateral twin pregnancies. [less ▲]

Detailed reference viewed: 62 (6 ULg)
Full Text
Peer Reviewed
See detailAccuracy of transrectal palpation for early pregnancy diagnosis in Egyptian buffaloes.
Karen, A. M.; Darwish, S.; Ramoun, A. et al

in Tropical Animal Health and Production (2011), 43(1), 5-7

The present study was undertaken to evaluate the accuracy of transrectal palpation (TRP) for diagnosing early pregnancy in buffaloes and the false diagnoses of the TRP test by using the pregnancy ... [more ▼]

The present study was undertaken to evaluate the accuracy of transrectal palpation (TRP) for diagnosing early pregnancy in buffaloes and the false diagnoses of the TRP test by using the pregnancy-associated glycoprotein radioimmunoassay (PAG-RIA) test. Pregnancy was diagnosed in 168 buffalo-cows once by TRP and PAG-RIA test between days 31 and 55 after breeding. The sensitivity of TRP for detecting pregnant buffalo-cows was 37.5% at days 31-35, increased to 93.8% at days 46-50 and reached 100% at days 51-55 (P < 0.01). All cases of false negative diagnoses (n = 10) had PAG concentration higher than the threshold (>/=1.8 ng/mL) for diagnosing pregnancy. The specificity of TRP for detecting non-pregnant buffalo cows ranged between 90.9%, and 100% between days 31 and 55. All cases of false positive diagnoses (n = 5) made by TRP had PAG concentrations lower than the threshold for diagnosing pregnancy. It could be concluded that TRP is an accurate method for diagnosing pregnant and non-pregnant buffalo cows from day 46 after breeding. [less ▲]

Detailed reference viewed: 58 (3 ULg)
Full Text
Peer Reviewed
See detailAltered secretion of pregnancy-associated glycoproteins during gestation in bovine somatic clones.
Constant, Fabienne; Camous, Sylvaine; Chavatte-Palmer, Pascale et al

in Theriogenology (2011), 76(6), 1006-1021

Somatic nuclear transfer (NT) in cattle is often accompanied by severe placental anomalies, hypertrophy, and hydrallantois, which induce a high rate of pregnancy losses throughout gestation. These ... [more ▼]

Somatic nuclear transfer (NT) in cattle is often accompanied by severe placental anomalies, hypertrophy, and hydrallantois, which induce a high rate of pregnancy losses throughout gestation. These placental deficits are associated with an abnormal increase of the maternal plasma levels of pregnancy-associated glycoprotein (PAG), produced by the trophoblastic binucleate cells (BNC) of the placenta. The objective of this study was to analyze the origin of the abnormally elevated PAG concentrations in the peripheral circulation of NT recipients during pathological pregnancies. Concentrations of PAG were measured both in maternal blood, in chorionic and cotyledonary tissular extracts from control recipients (after artificial insemination, AI, or in vitro fertilization, IVF) and clone recipients on Day 32, Day 62, and during the third trimester of gestation. Three different radioimmunoassay (RIA) systems were used. One homologous RIA for PSP60, similar to bovine PAG-1 (PAG(67kDa)), and two heterologous RIA with PAG(67kDa) as standard and tracer, and antisera anti-caprine PAG (AS#706 and AS#708). Circulating and tissular concentrations of bovine placental lactogen (bPL), a glycoprotein also produced by BNC, were determined by RIA at the same stages. The number of BNC in the placental tissues was determined by cell counting after immunostaining with anti PSP60 antibody on tissue sections from control and NT pregnancies. Maternal plasma PAG concentrations were not different among groups on Day 32, but they were significantly higher in NT than in control pregnancies on Day 62 with all three RIA and during the third trimester with two RIA (RIA-PSP60 and RIA with AS#708). Circulating bPL concentrations were undetectable on Days 32 and 62 and were not different in the third trimester between NT and control pregnancies. Tissular amounts of PAG on total proteins were not different between the two groups at all stages studied. No difference was determined in the percentage of PSP60-positive BNC in placental tissues between controls and NT on Day 62 and during the third trimester of pregnancy. Western blots of tissular extracts from placenta showed no major molecular weight changes of PAG in NT pregnancies compared to controls. No differences in maternal circulation concentrations or tissular content of bPL were observed between control and NT pregnancies. In conclusion, the specific increase of PAG in maternal plasma concentrations during abnormal NT pregnancies do not result from a higher proportion of BNC, or an increased protein expression of PAG and could be due to changes in the composition of terminal glycosylation which result into a clearance decrease of PAG from the circulation. [less ▲]

Detailed reference viewed: 30 (6 ULg)
Full Text
Peer Reviewed
See detailThe pregnancy-associated glycoproteins. Clinical applications for pregnancy follow-up in ruminant species.
Barbato, O.; Melo de Sousa, Noelita ULg; Canali, C. et al

in Revista română de medicină veterinară (2011), 21(2), 117-126

Pregnancy diagnosis is an important part in reproduction management of ruminant and embryonic mortality has a substantial impact on the fertility of demestic animals. This article focus on the use of ... [more ▼]

Pregnancy diagnosis is an important part in reproduction management of ruminant and embryonic mortality has a substantial impact on the fertility of demestic animals. This article focus on the use of pregnancy-associated glycoproteins for pregnancy follow-up. [less ▲]

Detailed reference viewed: 27 (1 ULg)
Full Text
Peer Reviewed
See detailPlasma urea nitrogen in relation to pregnancy rate in dairy sheep
Karen, Aly; Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg et al

in Animal reproduction science (2011), 124(1-2), 69-72

The aim of this field study was to investigate the relationship of plasma urea nitrogen (PUN) with the pregnancy rate in lactating AwassixMerino ewes. One hundred and eighty-five AwassixMerino ewes were ... [more ▼]

The aim of this field study was to investigate the relationship of plasma urea nitrogen (PUN) with the pregnancy rate in lactating AwassixMerino ewes. One hundred and eighty-five AwassixMerino ewes were used in the present study. Ewes were fed a diet containing 17.4% crude protein and were milked twice a day by the milking machine. The ewes were synchronized for estrus by insertion of intravaginal sponges containing 30mg flurogestone acetate for 14 days. At the time of sponge removal each ewe was administered eCG (600IU). All ewes were inseminated twice with fresh semen into the external os of the cervix at 48 and 56h after sponge removal. The day of insemination was considered as Day 0 for calculating the gestational period. Blood samples were collected from each ewe at Days 0, 18 for measurement of PUN concentrations and at Day 22 after AI for measurement of pregnancy-associated glycoprotein (PAG) by radioimmunoassay (RIA). Thirty-eight ewes (20.5%) were confirmed pregnant by PAG-RIA test at Day 22 and by ultrasonography at Day 80. The mean (+/-S.D.) concentration of PUN in all ewes at Day 0 was 12.7+/-4.6mmol/L. There were non-significant differences in the level of PUN between pregnant and non-pregnant ewes at Days 0 (12.2+/-4.2mmol/L vs. 12.8+/-4.7mmol/L, respectively) and 18 (9.6+/-2.9mmol/L vs. 10.4+/-4.0mmol/L, respectively) after AI. Mean PUN concentrations decreased significantly from Day 0 to Day 18 after AI in both pregnant and non-pregnant ewes. By using logistic regression analysis, there was no effect of PUN concentrations on the probability of pregnancy occurrence in the studied ewes (odds ratio: 0.97; 95% confidence interval: 0.9-1.05; P=0.45). In conclusion, there was no evidence of a relationship between PUN concentration and pregnancy rate for lactating AwassixMerino ewes in the present study because of low pregnancy rate observed. [less ▲]

Detailed reference viewed: 66 (5 ULg)
Full Text
See detailLate embryonic and early fetal mortalities in dairy cows: the incidence and pregnancy protein profiles.
Karen, A.; Bajcsy, A. C.; Kovacs, R. et al

in Proceedings of the 20th International Congress of Hungarian Association for Buiatrics. (2010, October 20)

Detailed reference viewed: 14 (2 ULg)
Full Text
See detailEffect of a corpus luteum with or without a cavity on fertility in dairy cows after prostaglandin treatment
Repasi, A.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

in Proceedings of the Hungarian Association for Buiatrics (2010, October)

Detailed reference viewed: 15 (1 ULg)
Full Text
Peer Reviewed
See detailCould Western blot analysis be an alternative to radio-immunoassay for sheep pregnancy associated glycoproteins measurements ?
El Amiri, B.; El Abbadi, N.; Benjelloun, B. et al

in Reproduction in Domestic Animals (2010, September 15), 45(Suppl 3), 65

This study prospected whether Western blot analysis could be an alternative to radio-immunoassay (RIA) for sheep pregnancy diagnosis. Blood samples were taken from Boujaâd ewes (n= 9) and Boujaâd x D’man ... [more ▼]

This study prospected whether Western blot analysis could be an alternative to radio-immunoassay (RIA) for sheep pregnancy diagnosis. Blood samples were taken from Boujaâd ewes (n= 9) and Boujaâd x D’man (BD) cross breed (n= 12). The pregnancy associated glycoproeins (PAGs) were weekly monitored in plasma during the whole gestation and the first month postpartum by two RIA systems (El Amiri et al., 2007, Reprod Domest Anim 42:257-62). The highly immunoreactive samples derived from PAG profiles were tested using Western blot based on the same antiserum used in RIA. Each week (from the 13th to the 19th week), two pools of plasma samples (4 ml each) were taken from single and multiple lambing ewes respectively. Samples were submitted to a protein extraction at neutral pH, followed by ammonium sulfate (A.S.) precipitation, dialysis, and lyophilization. The placental extract was used as a check. Results revealed that ewes with multiple lambs presented numerically higher PAG concentrations. In both RIA systems, the high concentrations were recoded around the 19th week of gestation and the maximal concentrations varied from 120 to 700 ng/ml. In Western blot, the placental extracts reacted positively while the plasma samples did not give any positive reaction. In conclusion, the RIA remains the only sensitive method to measure PAGs in plasma. The present study strengthens the need to develop an ELISA kit as an alternative to RIA systems. [less ▲]

Detailed reference viewed: 55 (2 ULg)
Full Text
See detailMonitoring endocrine profiles for prediction of stillbirth in Holstein-Friesian dairy cattle
Szenci, O.; Bajcsy, A. C.; Brydl, E. et al

Conference (2010, September 03)

Detailed reference viewed: 13 (3 ULg)
Full Text
Peer Reviewed
See detailDiagnostico precoz de gestation en Ganado ovino mediante un kit enzimoimmunoanalisis (EIA) de la glicoproteina asociada a la gestation (PAG) plasmatica.
Alabart, J. L.; Lahoz, B.; Folch, J. et al

in Proceedings of the XXXV Congreso de la Sociedad Espanola de Ovinotechnia y Caprinotecnia (SEOC) (2010, September)

Detailed reference viewed: 23 (3 ULg)
Full Text
Peer Reviewed
See detailEffect of exogenous circulating anti-bPL antibodies on bovine placental lactogen measurements in foetal samples.
Alvarez Oxiley, Andrea Vivian ULg; Melo de Sousa, Noelita ULg; Hornick, Jean-Luc ULg et al

in Acta Veterinaria Scandinavica (2010), 52

BACKGROUND: The involvement of placental lactogen (PL) in the regulation of foetal growth has been investigated in different species by in vivo immunomodulation techniques. However, when circulating ... [more ▼]

BACKGROUND: The involvement of placental lactogen (PL) in the regulation of foetal growth has been investigated in different species by in vivo immunomodulation techniques. However, when circulating antibodies are present together with the hormone, the procedure for hormonal measurement becomes considerably complex. The aim of this study was the immunoneutralization of bovine placental lactogen (bPL) concentrations in bovine foetal circulation by direct infusion of rabbit anti-bPL purified immunoglobulins (IgG) via a foetal catheter (in vivo study). The ability of a RIA based on guinea pig anti-bPL antiserum, for the measurement of bPL concentrations in samples containing exogenous rabbit anti-bPL immunoglobulins, was also analyzed in in vitro and in vivo conditions. METHODS: Six bovine foetuses were chronic cannulated on the aorta via the medial tarsal artery. Infusion of rabbit anti-bPL IgG was performed during late gestation. Pooled rabbit anti-bPL antisera had a maximal neutralization capacity of 25 microg bPL/mL of immunoglobulin. Interference of rabbit anti-bPL immunoglobulin with radioimmunoassay measurement using guinea pig anti-bPL as primary antibody was first evaluated in vitro. Polyclonal anti-bPL antibodies raised in rabbit were added in foetal sera to produce 100 samples with known antibodies titers (dilutions ranging from 1:2,500 till 1:1,280,000). RESULT(S): Assessment of the interference of rabbit anti-bPL antibody showed that bPL concentrations were significantly lower (P < 0.05) in samples added with dilutions of rabbit antiserum lower than 1:80,000 (one foetus) or 1:10,000 (four foetuses). It was also shown that the recovery of added bPL (12 ng/mL) was markedly reduced in those samples in which exogenous rabbit anti-bPL were added at dilutions lower than 1:20,000. Concentrations of foetal bPL were determined in samples from cannulated foetuses. In foetuses 1 and 6, bPL concentrations remained almost unchanged (<5 ng/mL) during the whole experimental period. In Foetus 3, bPL concentrations decreased immediately after IgG infusion and thereafter, they increased until parturition. CONCLUSION(S): The use of a bPL RIA using a guinea pig anti-bPL as primary antiserum allowed for the measurement of bPL concentrations in foetal plasma in presence of rabbit anti-bPL IgG into the foetal circulation. Long-term foetal catheterization allowed for the study of the influence of direct infusion of anti-bPL IgG on peripheral bPL concentrations in bovine foetuses. [less ▲]

Detailed reference viewed: 121 (17 ULg)
Full Text
Peer Reviewed
See detailComparison of accuracy of sonography, rectal palpation, and pregnancy-associated glycoproteins tests for pregnancy diagnosis in cows
Gajewski, Z.; Petrajtis, M.; Melo de Sousa, Noelita ULg et al

in Proceedings of the XIth Middle European Buiatrics Congress (2010)

Detailed reference viewed: 46 (2 ULg)
Full Text
Peer Reviewed
See detailPrediction of stillbirth by monotoring endocrine and metabolic parameters in dairy cattle
Szenci, 0; Bajcsy, A. C.; Brydl, E. et al

in Reproduction in Domestic Animals (2010), 45(Suppl 3), 109

Detailed reference viewed: 22 (2 ULg)
Full Text
Peer Reviewed
See detailMonitoring endocrine profiles for prediction of stillbirth in Holstein-Friesian dairy cattle
Szenci, O.; Bajcsy, A. C.; Bryd, E. et al

Poster (2010)

Detailed reference viewed: 33 (9 ULg)
Full Text
Peer Reviewed
See detailNeospora caninum and coxiella burnetii seropositivity are related to endocrine pattern changes during gestation in lactating dairy cows.
Garcia-Ispierto, I.; Nogareda, C.; Yaniz, J. L. et al

in Theriogenology (2010), 74(2), 212-230

Q fever is a zoonotic infection caused by Coxiella burnetii that is endemic worldwide. Domestic ruminants are a source of infection for humans. Given the suggestion that the bacterium recrudesces during ... [more ▼]

Q fever is a zoonotic infection caused by Coxiella burnetii that is endemic worldwide. Domestic ruminants are a source of infection for humans. Given the suggestion that the bacterium recrudesces during pregnancy in cattle, this study was designed to determine whether C. burnetii infection affects hormonal patterns, such as progesterone, cortisol, pregnancy associated glycoproteins (PAG), and prolactin during gestation in lactating cows. Possible interactions with Neospora caninum were also explored. The study was performed on 58 gestating non-aborting cows. Blood samples for hormone determinations were collected on Days 40, 90, 120, 150, 180, and 210 of gestation. For antibody determinations, blood was collected at day 40 postinsemination and postpartum. By GLM repeated measures analysis of variance, we established the effects of production and reproductive variables as well as Coxiella and Neospora seropositivity related to changes on cortisol, PAG, progesterone, and prolactin levels. Coxiella antibody levels were significantly related to cortisol, PAG, and plasma progesterone concentrations, whereas Neospora seropositivity was linked to plasma progesterone concentrations. The interaction between Coxiella and Neospora seropositivity was correlated with cortisol and plasma progesterone levels, whereas the interaction seropositivity against C. burnetii-plasma cortisol concentration was related to plasma PAG levels. Finally, an effect of lactation number only was observed on plasma prolactin. Our findings suggest that both the N. caninum and C. burnetii infection or the presence of both modify endocrine patterns throughout gestation. Cows seropositive to both, Neospora and Coxiella, showed higher plasma progesterone levels than the remaining animals examined. Seropositivity to C. burnetii was associated with placental damage and diminishing PAG levels throughout the second half of gestation, along with increased plasma cortisol levels on Day 180 of gestation. [less ▲]

Detailed reference viewed: 54 (8 ULg)
Full Text
Peer Reviewed
See detailPregnancy-associated Glycoprotein Profile during the First Trimester of Pregnancy in Egyptian Buffalo Cows.
El-Battawy, K. A.; Melo de Sousa, Noelita ULg; Szenci, Otto et al

in Reproduction in Domestic Animals (2009), 44(2), 161166

Pregnancy-associated glycoprotein (PAG) concentrations were measured in buffalo cows starting from day 28 after breeding. Oestrus was synchronized in 10 buffaloes using two injections of 25 mg ... [more ▼]

Pregnancy-associated glycoprotein (PAG) concentrations were measured in buffalo cows starting from day 28 after breeding. Oestrus was synchronized in 10 buffaloes using two injections of 25 mg prostraglandin (PG)F(2alpha) (Lutalyse((R))) at a 11-day interval. Blood sampling was conducted nearly twice weekly. Results indicated that plasma PAG concentrations in non-pregnant buffaloes were low (<0.20 ng/ml) during the whole experimental period (day 28 to 103), while in pregnant animals plasma PAG levels increased from day 28 (4.48 +/- 0.92 ng/ml) until day 41 (27.27 +/- 6.74 ng/ml), remaining high (20.71 +/- 9.20 ng/ml) until day 103. Progesterone levels were significantly (p < 0.0001) higher in pregnant (3.51-4.80 ng/ml) than in non-pregnant buffaloes (0.28-1.52 ng/ml). A significant difference (p < 0.0001) in plasma PAG concentrations between pregnant and non-pregnant animals starting at day 28 after breeding suggests that PAG-radioimmunoassay could be suitable for pregnancy diagnosis in buffaloes during this period. In conclusion, PAG test offers the advantages that it requires a single plasma sample for early pregnancy diagnosis as well as the accuracy of the test for the detection of pregnancy as early as day 28. [less ▲]

Detailed reference viewed: 62 (7 ULg)
Full Text
Peer Reviewed
See detailDetection of placental lactogens in Swamp buffalo by radioimmunoassay technique.
Hanh, N. V.; Huu, Q. X.; Uoc, N. T. et al

in Reproduction, Fertility and Development (2009), 21(1), 152-153

Detailed reference viewed: 8 (3 ULg)