References of "Melo de Sousa, Noelita"
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See detailPregnancy-Associated Glycoprotein D-56 kDa [Fragment] from Dama dama placenta - Accession number C0HJD0
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein C-39 kDa [Fragment] from Dama dama placenta - Accession number C0HJC9
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein B-56 kDa [Fragment] from Dama dama placenta - Accession number C0HJC8
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein A-62 kDa [Fragment] from Dama dama placenta - Accession number C0HJC7
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPlasma concentrations of pregnancy-associated glycoproteins measured using anti-bovine PAG-2 antibodies on Day 120 of gestation predict abortion in dairy cows naturally infected with Neospora caninum.
Garcia-ispierto, I; Almeria, S; Serrano, B et al

in Reproduction in Domestic Animals (2013), 48

The present study sought to determine: (i) the effects of Neospora caninum infection and twin pregnancy on plasma pregnancy-associated glycoprotein-2 (PAG-2) concentrations throughout pregnancy and (ii ... [more ▼]

The present study sought to determine: (i) the effects of Neospora caninum infection and twin pregnancy on plasma pregnancy-associated glycoprotein-2 (PAG-2) concentrations throughout pregnancy and (ii) whether plasma PAG-2 concentrations could predict abortion in N. caninum-infected cows. The study was performed on a commercial Holstein-Friesian dairy herd in northeastern Spain and the final data included those recorded in 53 non-aborting and 19 aborting animals. Blood samples were collected immediately before pregnancy diagnosis (on Days 40, 90, 120, 150, 180 and 210 post-insemination) in non-aborting cows or until the time of abortion detection in aborting cows. General lineal models (GLM) repeated measures ANOVA revealed the different behavior of PAG-1 and PAG-2, and significant effects of Neospora seropositivity, cool season and twin pregnancy on plasma PAG-2 concentrations throughout gestation (between-subject effects). In addition, based on the odds ratios, the likelihood of abortion increased in Neospora-seropositive cows (by a factor of 7.0) compared to seronegative animals and decreased in cows with a high plasma PAG-2 concentration (>4.5 ng/ml) on Day 120 of pregnancy (by a factor of 0.24), compared to the remaining cows. In conclusion, there is a relationship between plasma PAG-2 concentrations and the risk of abortion in Neosporainfected dairy cows. Thus, plasma PAG concentrations measured using anti-boPAG-2 antiserum on Day 120 of gestation could serve as an indicator of the abortion risk in N. caninum infected animals; values <4.5 ng/ml indicating a high risk of abortion in chronically infected animals. [less ▲]

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See detailPurification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalis placentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females.
Barbato, Olimpia; Melo de Sousa, Noelita ULg; Barile, Vittoria Lucia et al

in BMC Veterinary Research (2013), 9

BACKGROUND: Pregnancy-associated glycoproteins (PAGs) were first described as placental antigens present in the blood serum of the mother soon after implantation. Here, we describe the purification of ... [more ▼]

BACKGROUND: Pregnancy-associated glycoproteins (PAGs) were first described as placental antigens present in the blood serum of the mother soon after implantation. Here, we describe the purification of several pregnancy-associated glycoproteins from water buffalo placenta (wbPAGs). A specific radioimmunoassay (RIA) was developed for early pregnancy diagnosis in buffalo species. RESULTS: Amino-terminal microsequencing of immunoreactive placental proteins allowed the identification of eleven wbPAGs sequences [Swiss-Prot accession numbers: P86369 to P86379]. Three polyclonal antisera (AS#858, AS#859 and AS#860) were raised in rabbits against distinct wbPAG fractions. A new RIA (RIA-860) was developed and used to distinguish between pregnant (n = 33) and non-pregnant (n = 26) water buffalo females. CONCLUSIONS: Our results confirmed the multiplicity of PAG expression in buffalo placenta. In addition, the RIA-860 system was shown to be sensitive, linear, reproducible, accurate and specific in measuring PAG concentrations in buffalo plasma samples from Day 37 of gestation onwards. [less ▲]

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See detailMonitoring the circadian rhythm of serum and salivary cortisol concentrations in the horse
Bohak, Zs; Szabo, F.; Beckers, Jean-François ULg et al

in Domestic Animal Endocrinology (2013), 45(1), 38-42

Daily fluctuations of cortisol concentration in the blood or saliva have been repeatedly reported. However, several contradictions in the existing literature appear on this subject. The present study was ... [more ▼]

Daily fluctuations of cortisol concentration in the blood or saliva have been repeatedly reported. However, several contradictions in the existing literature appear on this subject. The present study was performed to definitively establish options for testing adrenocortical function. To the best of our knowledge, this is the first study to evaluate parallel circadian rhythms in salivary and serum cortisol concentrations during a 24-h period. Twenty horses were examined under the same conditions. Blood and saliva samples were taken every 2 h for 24 h to determine the daily changes in cortisol concentrations of saliva and serum at rest and to determine the relationship between salivary and serum cortisol levels. Cosinor analysis of group mean data confirmed a significant circadian component for both serum and salivary cortisol concentrations (P < 0.001 in both cases). The serum cortisol circadian rhythm had an acrophase at 10:50 AM (95% CI, 10:00 AM-11:40 AM), a MESOR of 22.67 ng/mL, and an amplitude of 11.93 ng/mL. The salivary cortisol circadian rhythm had an acrophase at 10:00 AM (95% CI, 9:00 AM-11:00 AM), a MESOR of 0.52 ng/mL, and an amplitude of 0.12 ng/mL. We found a significant but weak association between salivary and serum cortisol concentrations; the Pearson correlation coefficient was 0.32 (P < 0.001). The use of salivary cortisol level as an indicator of hypothalamic-pituitary-adrenal axis activity may be warranted. However, the salivary cortisol levels are more likely to be correlated with free plasma cortisol than with the total plasma cortisol concentration. [less ▲]

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See detailRelationship between plasma progesterone and pregnancy-associated glycoprotein concentrations during early pregnancy in dairy cows
Barbato, O; Merlo, M; Celi, P et al

in Veterinary Journal (2013)

The relationship between the concentration of plasma progesterone (P4) during embryo attachment or at recognition of pregnancy, and that of pregnancy-associated glycoprotein (PAG) was assessed in dairy ... [more ▼]

The relationship between the concentration of plasma progesterone (P4) during embryo attachment or at recognition of pregnancy, and that of pregnancy-associated glycoprotein (PAG) was assessed in dairy cows. The outcome of artificial insemination (AI) was classified as positive (AI+), negative (AIÿ), or late embryonic mortality (EM) by measuring circulating PAG concentrations and by ultrasonography. Based on P4 concentrations at either day 21 or day 15, AI+ and EM cows were classified into ‘low’ (P4 concentrations < mean) and ‘high’ (P4 concentrations > mean) P4 groups. In both experiments, the threshold of P4 concentration between the ‘low’ and ‘high’ groups was approximately 6 ng/mL. PAG concentrations were lower in the ‘low’ group only when P4 concentrations were below the threshold. The study findings suggest that a possible P4 threshold exists below which PAG secretion may be impaired [less ▲]

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See detailPlacental and gastric aspartic proteinases: new insights from bovine species
Bella, Amina ULg; Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg

in Proceedings of the 2nd Scientific Meeting of the Faculty of Veterinary Medicine (2012, October 19)

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See detailAssessment of pregnancy-associated glycoprotein (PAG) concentrations in swamp buffalo samples from fetal and maternal origins by using interspecies antisera.
Nguyen, V. H.; Barbato, O.; Bui, X. N. et al

in Animal Science Journal (2012), 83(10), 683-689

Pregnancy-associated glycoproteins (PAG) constitute a large family of glycoproteins found in the outer placental epithelial cell layer of the placenta in Eutherian species. In ruminants, they are noted to ... [more ▼]

Pregnancy-associated glycoproteins (PAG) constitute a large family of glycoproteins found in the outer placental epithelial cell layer of the placenta in Eutherian species. In ruminants, they are noted to be structurally closely related among the different species. This study was designed to determine PAG concentrations in maternal and fetal plasma, allantoic and amniotic fluids in buffalo species. Antisera (AS) generated in rabbits against distinct PAG molecules were used in three RIA-PAG systems: RIA-1 (raised against bovine PAG67kDa; AS#497), RIA-2 (raised against caprine PAG55+62kDa; AS#706) or RIA-3 (raised against buffalo PAG; AS#859). Samples were collected at slaughterhouse (n = 67). PAG concentrations determined by RIA-2 gave significantly higher results in both allantoic and amniotic fluids (12.7 ± 2.1 ng/ml and 24.0 ± 7.3 ng/ml, respectively). Regarding maternal and fetal plasma, PAG concentrations obtained by RIA-2 (21.8 ± 2.4 ng/ml and 20.2 ± 2.5 ng/ml, respectively) and RIA-3 (25.0 ± 2.2 ng/mL and 21.9 ± 3.2 ng/ml, respectively) were higher than those obtained by RIA-1 (15.5 ± 1.4 ng/ml and 16.1 ± 1.8 ng/ml, respectively). The correlation among the three systems was very high. The study clearly reveals the ability of PAG-RIA systems to determine PAG concentration in swamp buffalo samples. [less ▲]

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See detailPregnancy-associated glycoprotein, chymosin and pepsinogen immunoreactivity of proteins extracted from fetal gastric tissue in bovine species.
Bella, Amina ULg; Melo de Sousa, Noelita ULg; Dehimi, M. L. et al

in Research in Veterinary Science (2012), 92(3), 378-386

The objective of this work was to investigate the expression of gastric aspartic proteinases in fundic and pyloric mucosa removed from bovine fetuses. For this purpose, fractions issued from classical ... [more ▼]

The objective of this work was to investigate the expression of gastric aspartic proteinases in fundic and pyloric mucosa removed from bovine fetuses. For this purpose, fractions issued from classical biochemical protocols were analyzed by proteolytic method, by PAG-RIA and by Western blot with the use of antisera raised against both pepsinogens and PAG. A strong reaction of proteins extracted from the fundic mucosa collected at the beginning of pregnancy was revealed with both anti-bPAG-I and anti-bPAG-II antisera, suggesting the expression of pepsinogen F in bovine species. Concerning pyloric mucosa, the analysis by Western blot highlighted a very strong immunoreaction with the anti-bovine chymosin serum. Amino-terminal sequencing allowed to identify bovine fetuin and albumin in fundic extracts, chymosin in the pyloric mucosa extracts, as well as some unknown proteins in both mucosa. Despite no N-terminal microsequence corresponding to the hypothetical pepsinogen F could be identified, it cannot be excluded that an existing bovine pepsinogen F-like molecule could be degraded during the purification procedure or that co-purified proteins could be responsible for masking its N-terminal microsequence. [less ▲]

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See detailThe inseminating bull and plasma pregnancy-associated glycoprotein levels were related to peripheral leukocyte counts during the late pregnancy/early postpartum period in high-producing dairy cows.
Abdelfatah-Hassan, A.; Almeria, S.; Serrano, B. et al

in Theriogenology (2012), 77(7), 1390-1397

The interest in the immunology and endocrinology aspects of the peripartum in dairy cows has been increasing in the last years, since the animal status during the pre- and postpartum period determines the ... [more ▼]

The interest in the immunology and endocrinology aspects of the peripartum in dairy cows has been increasing in the last years, since the animal status during the pre- and postpartum period determines the subsequent productive and reproductive performance of the animal. At parturition, a drop in the immune functions of the peripheral blood polymorph-nuclear cells (PMN) was observed after a peak of Pregnancy-Associated Glycoproteins (PAGs) and recently, the inseminating bull was found to affect the plasma levels of bovine Pregnancy-Associated Glycoproteins (bovine PAGs) in the pregnant Holstein-Friesian dairy cows. The present work was aimed to determine whether changes in leukocyte counts during the peripartum, indicative of the animal immune status, could be related to the inseminating bull and to the levels of plasma pregnancy-associated glycoproteins (PAGs). Ninety six clinically healthy, single pregnant cows were selected in a commercial dairy herd. Six blood samples (four prepartum and two postpartum) were obtained every two weeks, and total and differential blood cell counts were analyzed. Based on procedures of General Linear Model analysis of variance for repeated measures, the inseminating bull affected counts of total leukocytes and lymphocytes (P<0.001; between-subject effects) throughout the peripartum period. In addition, cows with high plasma levels of PAGs (>900 ρg/µL) on Day 262-268 of gestation had higher numbers of total leukocytes and neutrophils throughout the peripartum period (P<0.001; between-subject effects). Young animals (≤ 1 lactation) had higher total leukocyte and lymphocytes counts than older cows (two or more lactations) throughout the study period. These results showed a clear relationship between the inseminating bull and plasma PAG levels and peripheral leukocyte counts during the peripartum period of dairy cows. [less ▲]

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See detailInvestigations sur les protéines placentaires extraites des cotylédons fœtaux du Cervus elaphus
Fondja, E.; Okuyama, M.; Petrova, A. et al

Poster (2012, February)

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See detailÉtude de la cyclicité en relation avec le poids vif et l’état corporel chez les agnelles Ouled Djellal nées en automne dans la région Est de l’Algérie
Lamrani, F; Benyounes, A; Sulon, J et al

in Annales de Médecine Vétérinaire (2012), 156

The objective of the present work was to describe the cyclicity of Ouled Djellal ewes born one year before regarding their live weight and body condition score, during the autumn season. The study has ... [more ▼]

The objective of the present work was to describe the cyclicity of Ouled Djellal ewes born one year before regarding their live weight and body condition score, during the autumn season. The study has been driven in the region of Guelma, East of Algeria, on 104 ewes belonging to two farms. At the beginning of the study, ewes from farms A and B had similar ages but differed in live weight and body condition scores. The concentration of progesterone wasdetermined by radioimmunoassay. The level of spontaneous cyclicity, as well as the plasmatic level of the progesterone, were influenced significantly by both live weight and body condition score (P<0.0001). If the first was in favor of ewes with high body weight and body condition score, the second seemed to be higher in slim cyclic ewes. The body fatness appears to influence positively the age of puberty. [less ▲]

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See detailEffect of restraint stess on plasma concentrations of cortisol, progesterone and pregnancy associated-glycoproteins-1 in pregnant heifers during late embryonic development.
Szenci, O.; Karen, A.; Bajcsy, A. C. et al

in Theriogenology (2011), 76(8), 1380-1385

The aim of the present study was to evaluate the effect of restraint stress, which is commonly practised in the field, on plasma concentrations of cortisol, progesterone (P4) and bovine pregnancy ... [more ▼]

The aim of the present study was to evaluate the effect of restraint stress, which is commonly practised in the field, on plasma concentrations of cortisol, progesterone (P4) and bovine pregnancy-associated glycoprotein-1 (boPAG-1) in pregnant heifers between Days 30 to 40 of gestation. Twelve Holstein-Friesian heifers between Days 30 (Day 0 of experiment) and 40 (Day 10 of experiment) of pregnancy in a Hungarian dairy farm were used in the present study. The heifers were exposed to an acute stressor consisting of immobilisation (restraint stress) in a crush for 2 h (Group 1, n = 6) on Day 2 (Hour 48) and for 2 × 2 h (Group 2, n = 6) on Days 2 and 3 (Hour 72) of the experiment. Transrectal ultrasonography (7.5 MHz linear-array rectal transducer) was performed daily from Day 0 to Day 10 of the experiment to detect embryonic heartbeat or the fate of the conceptus. Blood samples were withdrawn before each ultrasonographic examination. Additional blood samples were withdrawn by 1 and 2 h (at Hours 49 and 50 in Groups 1 and 2 and Hours 73 and 74 in Group 2) of the onset of applying the stressor. Plasma cortisol, P4 and boPAG-1 concentrations were measured by radioimmunoassay. Acute restraint stress significantly (P < 0.001) increased the plasma cortisol level in pregnant heifers at 1 h of the exposure to the stressor at Days 2 (48 h) and 3 (72 h) of the experiment. On the other hand, the restraint stress did not affect the concentration of P4 and boPAG-1 concentrations in both groups. In conclusion, restraint stress for 2 h during early pregnancy in heifers increased blood cortisol, but it did not affect the concentrations of P4 and boPAG-1 between Days 30 to 40 of gestation. [less ▲]

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See detailDevelopment of a new radioimmunoassay by using antiserum against pregnancy-associated glycoprotein II : concentrations in holstein dairy cattle .
Bella, Amina ULg; Melo de Sousa, Noelita ULg; Lopez-Gatius, F. et al

in Proceedings of the 6th European Congress of Bovine Health and Management (ECBHM) (2011, September 07)

Bovine pregnancy-associated glycoproteins (boPAG) can be classified into two main subfamilies: the boPAG–1 and the boPAG–2 groups. RIA systems allow quantifying boPAG-1 molecules in pregnant cattle ... [more ▼]

Bovine pregnancy-associated glycoproteins (boPAG) can be classified into two main subfamilies: the boPAG–1 and the boPAG–2 groups. RIA systems allow quantifying boPAG-1 molecules in pregnant cattle. Concerning boPAG-2, as no radioimmunoassay was developed, its quantification in peripheral blood of pregnant cows remains to be investigated. The present work aimed to develop a new heterologous RIA allowing the measurement of boPAG-2 concentrations in bovine species. A total of 77 Holstein dairy cows were used for this study. They were bled at Days 45, 90, 120, 150, 180, 210 after AI, at parturition and at Day 30 postpartum. Polyclonal antiserum against boPAG-2 (AS438) was raised in New Zealand rabbits. Due to the instability of boPAG-2 molecule, we used boPAG-1 (67 kDa) as standard (dilutions ranging from 100 to 0.8 ng/mL) and for iodination with 125-I isotope. The optimal dilution for primary AS438 was 1:1,1500. Mean concentrations of boPAG-2 increased continuously from Day 45 of pregnancy (0.78 ± 0.07 ng/mL) reaching a peak at Day 210 (32.78 ± 3.02 ng/mL). Thereafter, they decreased until parturition (7.73 ± 0.59). Lower PAG concentrations were observed at Day 30 postpartum (3.62 ± 0.31 ng/mL). In conclusion, boPAG-2 profile differed from boPAG-1. In general, concentrations of boPAG-2 reached lower values, with no dramatic increase being observed at peripartum period. Due to the lower postpartum concentrations, it is expected that this new RIA can be available for pregnancy diagnosis of cows inseminated in early postpartum, minimizing the risk of false positive concentrations due to the previous pregnancy. [less ▲]

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See detailConcentrations of pepsinogens A and C in peripheral blood of cattle at different developmental stages.
Melo de Sousa, Noelita ULg; Michiels, Jean-Albert ULg; Terroni, D. et al

in Proceedings of the 6th European Congress of Bovine Health and Management (ECBHM) (2011, September 07)

Measurements of global proteolytic activity of pepsinogens are successfully used for diagnosis of gastric nematode parasitism in cattle. Higher pepsinogen concentrations detected in peripheral circulation ... [more ▼]

Measurements of global proteolytic activity of pepsinogens are successfully used for diagnosis of gastric nematode parasitism in cattle. Higher pepsinogen concentrations detected in peripheral circulation were found to be associated with damage in gastric mucosa The aim of the present study was to investigate the ontogenesis of PgnA and PgnC in cattle. Bovine PgnA and PgnC were isolated in our laboratory. Both antigens were labeled to 125I by using chloramine T method. Standard curves ranged from 125 to 1.0 ng/mL. Antisera were raised in rabbits: AS866 (anti-PgnA) and AS869 (anti-PgnC). The optimal dilutions for primary antisera were 1:15,000 and 1:20,000 for AS866 and AS869, respectively. In order to establish the ontogenesis of both PgnA and PgnC, samples were collected from 50 bovine fetuses (3rd to the 9th month), 18 calves and 57 adult cattle. Mean(±SEM) concentrations of PgnA were 4.6±0.7 ng/mL, 78.9±6.7 ng/mL and 133.2±17.6 ng/mL in fetuses, calves and mature cows, respectively. Concentrations of PgnC were <0.9 ng/mL, 13.5±1.1 ng/ml and 201.5±26.5 ng/ml in fetuses, calves and mature cows, respectively. The ratio PgnA/PgnC concentrations decrease from fetal age (maximal ratio due to undetectable PgnC concentrations), being 5.8 in young calves and lowest (0.7) in mature cows. In conclusion, PgnA and PgnC concentrations can be distinctly measured by specific RIA systems. Ratio PgnA/PgnC concentrations can be useful to better understand the ontogenesis of gastric aspartic proteinases in cattle. It is expected that clinical application of these assays will help vet practitioners to establish diagnosis of gastric nematode parasitism in dairy and beef herds taking into account the age of the animal. [less ▲]

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See detailPregnancy-associated glycoprotein levels in cattle exhibiting prolonged pregnancy: case reports.
Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg

in Proceedings of the 6th European Congress of Bovine Health and Management (ECBHM) (2011, September 07)

Prolonged gestation (PG) is a complex clinical entity which occurs in bovine species. This pathology has been described in Belgian Blue cattle. PG may require termination by cesarean section. Fetuses ... [more ▼]

Prolonged gestation (PG) is a complex clinical entity which occurs in bovine species. This pathology has been described in Belgian Blue cattle. PG may require termination by cesarean section. Fetuses often exhibit increased body weight, long hair and well erupted teeth. All calves die within minutes or hours after birth. From endocrinological point of view, PG is highly correlated to fetal adenohypophyseal aplasia. The aim of the present study was to investigate the usefulness of PAG RIA for diagnosis of prolonged gestation in Belgian Blue cattle. Blood samples were collected from five Blue Belgian cows having an abnormally long pregnancy length (10 to 15 months). PAG was measured by routine RIA with the use of antiserum AS497 as primary antibody. A purified bovine PAG preparation (boPAG67kDa) was used as standard (0.8 to 100 ng/mL) and for radio-iodination (125I). Cesarean section was used for termination of pregnancy of those cows exhibiting abnormal PAG concentrations. Morphological aspect and viability of fetuses were reported. Concentrations of PAG were very low (94.1 ng/mL, 50 ng/mL, 5 ng/mL, 110 ng/mL and 26 ng/mL) in the five females suffering of prolonged gestation. Fetuses issued from prolonged gestation exhibited long hair, well erupted teeth and died within few minutes after birth. In conclusion, PAG concentration is a good indicator of prolonged gestation in cattle. Concentrations are much lower than those expected in normal ongoing pregnancy (<120 ng/mL versus >1,000 ng/mL). [less ▲]

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