Fatty Acid Binding Protein [Fragment] from Ovis aries placenta - Access number P83204

E-print/Working paper (2002)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

Pregnancy-Associated Glycoprotein 67 [Fragment] from Bos indicus placenta (Access number P83126)

E-print/Working paper (2002)

N-terminal microsequence obtained after purification and characterization of placental proteins in zebu cattle. Proteins were submitted to SwissProt databank.

Pregnancy-Associated Glycoprotein 57 [Fragment] from Ovis aries placenta - Access number P83203

E-print/Working paper (2002)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

Pregnancy-Associated Glycoprotein 59 [Fragment] from Ovis aries placenta - Access number P83444

E-print/Working paper (2002)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

Pregnancy-associated glycoprotein concentrations during pregnancy and postpartum periods in zébu cattle.

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(1), 10-11

Investigations nouvelles sur les protéines associées à la gestation chez la brebis en vue d’application clinique.

Poster (2002)

Purification and characterization of a pregnancy-associated glycoprotein (ovPAG-6) from sheep placenta removed between 66 to 100 days of gestation.

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(1), 12-13

Potential influence of pregnancy hormones and proteins synthesized during pregnancy on the maternal immunological status

in Annales de Médecine Vétérinaire (2002), 146(2), 71-82

During pregnancy, the endocrine function of the gonads and the foeto-placental unit involves the synthesis of several hormones (progesterone, oestrogens, cortisol, prostaglandins, prolactin, chorionic ... [more ▼]

During pregnancy, the endocrine function of the gonads and the foeto-placental unit involves the synthesis of several hormones (progesterone, oestrogens, cortisol, prostaglandins, prolactin, chorionic gonadotropin, placental lactogen, also designed "chorionic somatomammotropin", etc.), and a series of growth factors and proteins. Some proteins and glycoproteins synthesized during gestation interfere with the establishment of pregnancy, corpus luteum maintenance, intermediate maternal metabolism, fetal growth, mammary growth and immunotolerance of the conceptus by the mother. This review includes a presentation of the main pregnancy-related hormones and proteins and their hypothetical role on the immunological status of the mother. [less ▲]

Dosages réalisables pour le diagnostic de gestation

in Journées Européennes de la Société Française de Buiatrie 2002 (2002)

Lorsque les signaux embryonnaires de la gestation induisent une modification du côté maternel, une possibilité nouvelle s’ouvre aux chercheurs attentifs au développement de tests pour le diagnostic ou le ... [more ▼]

Lorsque les signaux embryonnaires de la gestation induisent une modification du côté maternel, une possibilité nouvelle s’ouvre aux chercheurs attentifs au développement de tests pour le diagnostic ou le suivi de la gestation. Dans ce chapitre, nous essayerons de passer en revue les différentes molécules dont le dosage ou la mise en évidence a fait l’objet de publications scientifiques en relation avec le diagnostic de la gestation. Dans la mesure du possible, nous envisagerons les différents marqueurs en suivant la chronologie de leur apparition au cours de la gestation, mais ce critère ne prendra pas un caractère absolu car souvent plusieurs molécules se recouvrent, ou encore leur apparition au niveau de placenta précède largement le moment où elles apparaissent dans le sang maternel en quantités mesurables. Nous essayerons également, de connecter chaque molécule avec l’application que son dosage permet dans la clinique vétérinaire. Pour terminer, nous ferons une description rétrospective de quelques cas cliniques bien documentés qui montrent l’importance de l’association du dosage de protéines associées à la gestation et de la progestérone pour arriver à une meilleure compréhension des échecs observés dans le déroulement de la gestation. [less ▲]

Aspartic proteinase members secreted by the ruminant placenta: Specificity of three radioimmunoassay systems for the measurement of pregnancy-associated glycoproteins

in Reproduction in Domestic Animals (2002), 37(6), 324-329

Pregnancy-associated glycoproteins (PAGs) isolated from the placenta of various ruminant species are enzymatically inactive members of the aspartic proteinase family. The measurement of these proteins in ... [more ▼]

Pregnancy-associated glycoproteins (PAGs) isolated from the placenta of various ruminant species are enzymatically inactive members of the aspartic proteinase family. The measurement of these proteins in the maternal blood can be a good indicator of the presence of a live embryo. As certain aspartic proteinases are present in biological fluids in physiological and pathological conditions at various concentrations, it was necessary to determine the specificity of three radioimmunoassay (RIA) systems currently used for the detection of PAG molecules. Commercially available members of the aspartic proteinase family like pepsinogen, pepsin, chymosin, rennet, cathepsin D and renin were tested in a wide concentration range (10 ng/ml - 1 mg/ml). Pepsinogen cross-reacted in RIA 1, RIA 2 and RIA 3 over 1 mg/ml, 50 microg/ml and 500 microg/ml concentrations, respectively. In the presence of pepsin, cross-reaction was observed in RIA 1, RIA 2 and RIA 3 over 1 mg/ml, 500 microg/ml and 1 mg/ml concentrations, respectively. Chymosin and rennet could cross-react in RIA 2 and RIA 3, while renin and cathepsin D did not decrease the binding of the tracer to antisera more, than that of the minimal detection limit. As the plasma/serum concentrations of the examined aspartic proteinases reported in the literature were outside the concentration range where cross-reaction was observed, it can be concluded that these RIA systems were specific for the detection of PAGs in biological fluids. [less ▲]