References of "Melo de Sousa, Noelita"
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See detailBreed-cross pregnancies increased plasmatic pregnancy-associated glycoprotein (PAG) concentration in pregnant Neospora-seropositive dairy cows
López-Gatius, Fernando; Garbayo, J. M.; Serrano, B. et al

in Reproduction in Domestic Animals (2006), 41(suppl 2), 112

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See detailPregnancy-associated glycoprotein (PAG) profiles during the peri-implantation period in recipients carrying bovine somatic clones: preliminary results
Heyman, Yvan; Chavatte-Palmer, P.; Melo de Sousa, Noelita ULg et al

in Reproduction, Fertility and Development (2005), 17(1-2), 168

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See detailInduction des chaleurs chez le zebu Goudali : observation de l’oestrus et insémination artificielle.
Pitala, W.; Zongo, M.; Boly, H. et al

Scientific conference (2005)

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See detailPregnancy associated glycoprotein-1,-6,-7, and-17 are major products of bovine binucleate trophoblast giant cells at midpregnancy
Klisch, K.; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

in Molecular Reproduction and Development (2005), 71(4), 453-460

Pregnancy associated glycoproteins (PAGs) are extensively glycosylated secretory proteins of ruminant trophoblast cells. In cattle placenta several PAG cDNAs are expressed, but the variety of ... [more ▼]

Pregnancy associated glycoproteins (PAGs) are extensively glycosylated secretory proteins of ruminant trophoblast cells. In cattle placenta several PAG cDNAs are expressed, but the variety of correspondent proteins and their degree of glycosylation are not well characterized. Thus, we purified PAGs by using a protocol which included a lectin (Vicia villosa agglutinin) affinity chromatography. Due to their specific glycosylation pattern, PAGs derived from binucleate trophoblast giant cells were highly enriched by this protocol. PAGs were purified from cotyledons of 2 day 100 placentas and from a single placenta at day 155 and 180. In all samples three major bands (75; 66; 56 kDa) were detected by one-dimensional SDS-PAGE. Mass-spectrometric analysis identified the 75 kDa band as a mixture of PAG-7 and PAG-6, the 66 kDa band as PAG-1 and the 56 kDa band as PAG-17. N-terminal sequencing of the day 100 sample confirmed the mass spectrometric identifications. Enzymatic release of N-glycans with peptide-N-glycanase-F from PAGs reduced the molecular weight to approximately 37 kDa which corresponds to the theoretical molecular mass of PAGs. Limited peptide-N-glycanase-F treatment revealed that all four N-glycosylation sites are quantitatively occupied in PAG-1. Compared to PAG-1 the number of potential N-glycosylation sites is lower in PAG-17 (three sites) and higher in PAG-6 and -7 (five and six sites, respectively). This suggests that the number of attached N-glycans is the main determinant of molecular mass of bovine PAGs. The degree of glycosylation may be a major factor regulating the plasma half life of PAGs. [less ▲]

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See detailPregnancy-Associated Glycoprotein (PAG) profiles during the peri-implantation period in recipients carrying bovine somatic clones: preliminary results
Heyman, Y; Chavatte-Palmer, P; Melo de Sousa, Noelita ULg et al

in Journal of Reproduction & Fertility. Abstract Series (2004), 17(2), 168169

Bovine pregnancy-associated glycoproteins (bPAGs) are secreted by binucleate cells of the placenta and can be assayed in maternal plasma as indicators of pregnancy and markers for the functional status of ... [more ▼]

Bovine pregnancy-associated glycoproteins (bPAGs) are secreted by binucleate cells of the placenta and can be assayed in maternal plasma as indicators of pregnancy and markers for the functional status of the trophoblast. The concentrations of PAGs vary differentially during the peri-implantation period. Large offspring syndrome (LOS) and abnormal placentation have been associated in cloned fetuses with abnormally increased pregnancy-specific protein 60 (PSP60). The aim of this study was to investigate the evolution of plasma levels of PAGs measured by the use of three different RIA systems in heifers after transfer of somatic cloned embryos and to compare that to plasma levels of control recipients during early pregnancy to distinguish which ones vary differentially. Cloned embryos were derived from nuclear transfer from fibroblasts of an adult cell line to in vitro-matured bovine oocytes according to Vignon et al. (1998 Theriogenology 49, 392). Control embryos were obtained in vitro after IVF and cultured in serum-free medium up to the blastocyst stage. By Day 7, cloned (n = 29) and control embryos (n = 10) were transferred to synchronous recipient heifers of the Normande breed (one embryo per recipient). Blood samples were taken every 2–3 days between Days 25 and 50 of pregnancy from recipients that had a positive progesterone test on Day 21, and the plasma was stored frozen until assay. Pregnancy status was monitored by repeated ultrasound scanning from Day 35 to Day 90. Concentrations of PAGs were determined by validated RIA assays for the different forms (PAGI67, PAG55+62, PAG55+59) using 3 antisera (AS 497, AS 706, AS 708, respectively) (Perenyi et al. 2002 Reprod. Domest. Anim. 37, 100–104). profiles of only the recipients that were confirmed pregnant by scanning over Day 50 (n = 18 clones, n = 4 controls) were analyzed and compared. Concentrations of PAGs measured by AS 497 and AS 706 were significantly higher in clones than in control recipients during the second month of pregnancy, indicating that the placenta of clones secreted these forms in a different manner compared to controls. Moreover, we found that concentrations of PAGs as determined with the two same antisera were higher for recipients with a cloned fetus that developed to term compared to those which had fetal loss before 3 months. For instance, using the AS 706, respective values of PAGs at Day 45 were 17.64 ± 7.59 and 10.96 ± 6.38 ng/mL for pregnancies with cloned and control fetuses, respectively, (P < 0.05) and 21.29 ± 4.77 and 12.88 ± 8.90 ng/mL in pregnancies with cloned fetuses that developed normally to term or died before 3 months of age, respectively, (P < 0.05). We conclude that our assays using two antisera could be a predictive test for fetal loss in clone pregnancies. Until now, recipients carrying cloned embryos that develop LOS during late pregnancy could not be detected by conventional assays in maternal serum or by scanning during the period of 35–50 days. This study provides a new diagnostic tool to detect them. Investigations are in progress to check the localization of these different forms of PAG in placentomes removed from recipients carrying somatic clones. [less ▲]

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See detailEffects of an experimental Trypanosoma congolense infection on the reproductive performance of West African Dwarf goats.
Faye, Dethie; Sulon, Joseph ULg; Kane, Yaghouba et al

in Theriogenology (2004), 62(8), 1438-51

Thirty-six West African Dwarf (WAD) goats were used to assess the effects of an experimental Trypanosoma congolense infection on their reproductive system. Estrous cycles were synchronised and when ... [more ▼]

Thirty-six West African Dwarf (WAD) goats were used to assess the effects of an experimental Trypanosoma congolense infection on their reproductive system. Estrous cycles were synchronised and when confirmed pregnant (n = 31), the does were randomly allocated into control and trypanosome-infected groups. After infection, the animals were carefully observed till parturition. Trypanosome infection caused an increase of rectal temperature, a significant drop in PCV (infected: 23.3 +/- 0.3%; control: 28.5 +/- 0.4%; P < 0.0001) and abortions in 27.8% of the infected does. Kids born from infected does had a lower birth weight than kids born from control goats (0.9 +/- 0.1 kg versus 1.6 +/- 0.1 kg; P < 0.0001). Eight out of 13 kids (61.5%) that were born alive from infected does died during their first week of life. Plasma pregnancy-associated glycoprotein (PAG) and progesterone concentrations were lower in the infected animals than in the controls. In general, PAG concentration in does which aborted dropped before abortion. Our results revealed that artificial T. congolense infection affected reproductive performance of WAD goats with abortions, premature births and perinatal losses being observed. Neither transplacental transmission of T. congolense nor histopathological lesions of the placenta could be demonstrated. [less ▲]

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See detailRadioimmunoassay of bovine placental lactogen (bPL) using an antiserum raised in guinea pigs: measurement in foetal plasma
Alvarez-Oxiley, A. V.; Melo de Sousa, Noelita ULg; Touati, Kamal ULg et al

in Reproduction (Cambridge, England). Abstract Series (2004), 31

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See detailUse of pregnancy specific proteins, P4, PGFM and E1S assays to monitor pregnancy in sheep
Gajewski, Z.; Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg et al

in Reproduction in Domestic Animals (2004), 39(4), 288

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See detailInvestigation on prenatal endocrinology: preliminary results on long term catheterisation of bovine foetuses
Touati, Kamal ULg; Melo de Sousa, Noelita ULg; Gangl, M. et al

in Reproduction (Cambridge, England). Abstract Series (2004), 31

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See detailApplication de l'échographie à l'étude de la dynamique folliculaire à l'oestrus induit chez les femelles zébu "Goudali"
Pitala, W.; Boly, H.; Coulibaly, I. et al

in Tropicultura (2004), 22(3), 110-115

L’échographie transrectale a été utilisée pour suivre la dynamique folliculaire chez 8 femelles zébu ‘Goudali’. Les animaux ont été répartis comme suit: Groupe 1: utilisation d’implant Crestar combiné à ... [more ▼]

L’échographie transrectale a été utilisée pour suivre la dynamique folliculaire chez 8 femelles zébu ‘Goudali’. Les animaux ont été répartis comme suit: Groupe 1: utilisation d’implant Crestar combiné à PGF2 et PMSG (n= 4); Groupe 2: implant Crestar combiné à PMSG (n= 2) et Groupe 3: implant Crestar combiné à PGF2 (n= 2). L’échographie a été réalisée le jour de la pose des implants, au moment du retrait, et à intervalle de 4 heures entre la 28e et la 56e heure après retrait. La détermination du nombre de corps jaunes a été faite 7 jours après l’ovulation. Le jour du retrait, le nombre de follicules de diamètre compris entre 2-3 mm a été de respectivement 5,8 ± 2,2; 3,5 ± 0,7 et 4,5 ± 0,7 pour les Groupes 1, 2 et 3. Au début des chaleurs (30,4 ± 4,9 heures après le retrait des implants); 4,1 ± 1,3 follicules ayant un diamètre moyen de 5-6 mm ont été observées pour les trois groupes. Une moyenne de 1,1 ± 0,4 follicules dominants (> 8 mm) a été observée à partir de la 36e heure après la fin du traitement. Le diamètre des follicules pré-ovulatoires n’a pas différé entre les groupes. Les premières ovulations ont été observées entre 22 et 26 heures après le début des chaleurs. [less ▲]

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See detailEmbryonic signals and pregnancy diagnosis in ruminants
Beckers, Jean-François ULg; Touati, Kamal ULg; Alvarez-Oxiley, A. V. et al

in Reproduction in Domestic Animals (2004), 39(4), 252

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See detailDiagnostic et suivi de gestation chez la chèvre et la brebis
Melo de Sousa, Noelita ULg; Gonzalez, F.; Karen, A. et al

Scientific conference (2004)

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See detailIsolation and characterization of eight pregnancy-associated glycoproteins present at high levels in the ovine placenta between day 60 and day 100 of gestation
El Amiri, B.; Remy, Benoit; Melo de Sousa, Noelita ULg et al

in Reproduction Nutrition Development (2004), 44(3, May-Jun), 169-181

Pregnancy-associated glycoproteins (PAG), structurally related to aspartic proteinases, are expressed in the outer epithelial cell layer (chorion/trophectoderm) of the ungulate placenta. The aim of the ... [more ▼]

Pregnancy-associated glycoproteins (PAG), structurally related to aspartic proteinases, are expressed in the outer epithelial cell layer (chorion/trophectoderm) of the ungulate placenta. The aim of the present study was to isolate as many PAG molecules as possible from placentae collected between day 60 and day 100 of gestation and to characterize their amino-terminal amino-acid sequences. Three heterologous radioimmunoassays were used to monitor PAG immunoreactivity throughout the isolation procedures. Sequential use of DEAE-cellulose, gel filtration, and CM ceramic chromatographies led to the isolation of several fractions rich in PAG immunoreactivity. The fractions with a large amount of proteins were also purified by chromatofocusing. The analysis of immunoreactive fractions by SDS-PAGE, Western blotting and two-dimensional electrophoresis followed by amino-terminal microsequencing on PVDF membranes allowed to identify eight different ovPAG with apparent molecular masses ranging from 55 to 66 kDa and isoelectric points from 4.0 to 6.8. The N-terminal sequences were determined and their comparison to those previously identified revealed that four of them are identical to those encoded by previously known cDNA, while the additional four sequences appear to be novel since they have not yet been described. [less ▲]

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See detailEarly pregnancy diagnosis in sheep by progesterone and pregnancy-associated glycoprotein tests
Karen, A.; Beckers, Jean-François ULg; Sulon, Joseph ULg et al

in Theriogenology (2003), 59(9), 1941-1948

The aim of this study was to compare the accuracy of the progesterone (P4) and pregnancy associated glycoprotein (PAG) tests for determination of early pregnancy in sheep. Estrus was synchronized in 182 ... [more ▼]

The aim of this study was to compare the accuracy of the progesterone (P4) and pregnancy associated glycoprotein (PAG) tests for determination of early pregnancy in sheep. Estrus was synchronized in 182 Awassi x Merino ewes and blood samples were collected at Days 0 (day of the insemination), 18, 22, 29, 36, and 50 after artificial insemination (AI). Plasma P4 concentrations at Days 0 and 18 were determined by double antibody radioimmunoassay, while PAG concentrations at Days 22, 29, 36 and 50 were determined by a heterologous, double-antibody radioimmunoassay (RIA) using the bovine PAG 67 kDa subunit as tracer and standard and rabbit antiserum raised against a mixture of caprine 55 and 59 kDa PAG subunits as the first antibody. The discriminatory value for diagnosis of pregnancy by the P4 and the PAG-RIA tests was greater than or equal to1 ng/ml. Based on lambing data, the accuracy for diagnosing pregnant (sensitivity) and non-pregnant ewes (specificity) and predictivity of both tests were calculated. The sensitivity, specificity, positive and negative predictive values for P4 and PAG tests were 100, 95.4, 81.6, and 100% at Day 18 (P4) and 93.5, 100, 100 and 98.7% at Day 22 (PAG), respectively. For diagnosis of non-pregnant ewes the PAG test had significantly higher specificity than the P4 test (P < 0.05). It is concluded that ovine pregnancy can be reliably diagnosed at Day 22 after AI by using a heterologous radioimmunoassay of PAG. [less ▲]

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See detailAn improved radioimmunoassay for measurement of pepsinogen in porcine blood samples
Banga-Mboko, Henri; Sulon, Joseph ULg; Lange, J. M. et al

in Veterinary Journal (2003), 165(3), 288-295

The study was conducted to develop a sensitive and specific radioimmunoassay (RIA) for the measurement of pepsinogen in porcine serum, and to use this test for the determination of pepsinogen ... [more ▼]

The study was conducted to develop a sensitive and specific radioimmunoassay (RIA) for the measurement of pepsinogen in porcine serum, and to use this test for the determination of pepsinogen concentrations in serum samples from fetuses and pigs of different ages. Compared to a previously described RIA, major improvements were made concerning the use of specific polyclonal antibodies and the use of an appropriate buffer. The assay was able to detect pepsinogen concentrations of >/=0.2 ng/mL. The recovery of pepsinogen was close to 95%. The intra-assay coefficients of variations ranged between 3.9 and 7.5% whereas the interassay ranged between 8.8 and 11.9%. These percentages correspond to a satisfactory accuracy and reproducibility of the assay. No cross-reactions were observed with the main commercially available products of the aspartic proteases family except porcine pepsin cross-reacted over 62.5 microg/mL. Pepsinogen concentrations increased steadily with increasing age of the fetuses and the pigs (P<0.05). Pepsinogen concentrations (+/-SE) in fetuses of 90-100 (n=24) and 100-110 days of pregnancy (n=36) were 0.5+/-0.1 and 5.3+/-1.3 ng/mL, respectively. In pigs of 21, 98, and 213 days of age, the pepsinogen concentrations were 290.6+/-10.8, 343.1+/-17.9 and 383.5+/-15.3 ng/mL, respectively. The results demonstrate that RIA is accurate and can be used easily to assess pepsinogen concentrations in pig sera. The test may constitute a valuable tool in epidemiological surveys and in studies related to gastric diseases in pigs. [less ▲]

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See detailPregnancy in rabbits actively immunized against bovine pregnancy-associated glycoprotein 1
Banga-Mboko, Henri; Halloy, D.; Perenyi, Zsolt et al

in Fertility and Sterility (2003), 79(1), 226-227

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See detailValidation of two homologous radioimmunoassays for measuring pregnancy-associated glycoprotein in ewes.
El Amiri, B.; Karen, A.; Sulon, J. et al

in Reproduction in Domestic Animals (2003), 38(4), 358

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See detailPregnancy-Associated Glycoprotein 55h [Fragment] from Ovis aries placenta - Access number P83500
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein 59g [Fragment] from Ovis aries placenta - Access number P83499
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein 60f [Fragment] from Ovis aries placenta - Access number P83498
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

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