Pregnancy-Associated Glycoprotein 59g [Fragment] from Ovis aries placenta - Access number P83499

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

Pregnancy-Associated Glycoprotein 66e [Fragment] from Ovis aries placenta - Access number P83497

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

Pregnancy-Associated Glycoprotein 58c [Fragment] from Ovis aries placenta - Access number - P83495

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

Pregnancy-Associated Glycoprotein 58a [Fragment] from Ovis aries placenta - Access number P83493

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

Pregnancy-associated glycoprotein concentrations in plasma and milk samples for early pregnancy diagnosis in Lacaune dairy sheep.

in Reproduction in Domestic Animals (2003), 38(4), 322

Progesterone, cortisol and pregnancy-associated glycoprotein concentrations in three aborted cows

in Proceedings of the 8e Journée de rencontre Bioforum (2003)

Diagnostic et suivi de la gravidité chez le zébu par échographie

in Revue d'Elévage et de Médecine Vétérinaire des Pays Tropicaux (2003), 56(3-4), 171-176

Des diagnostics de gravidité ont été réalisés chez 64 femelles zébus de races Peuhl et Goudali (Bos indicus) aux 28è et 40è jours postinsémination à l'aide d'un échographe muni d'une sonde linéaire de 6 ... [more ▼]

Des diagnostics de gravidité ont été réalisés chez 64 femelles zébus de races Peuhl et Goudali (Bos indicus) aux 28è et 40è jours postinsémination à l'aide d'un échographe muni d'une sonde linéaire de 6 MHz. Au 28è jour, l'exactitude globale a été de 92,2 p. 100, avec une exactitude de 92,6 p. 100 et 91,9 p. 100 respectivement pour les diagnostics positif et négatif. Au 40è jour, l'exactitude globale a été de 98,4 p. 100, avec une exactitude de 100 p. 100 et 97,3 p. 100 respectivement pour les diagnostics positif et négatif. Les fiabilités ont varié de 89,2 p. 100 (fiabilité du diagnostic de gravidité positif au 28è jour) à 100 p. 100 (fiabilité du diagnostic de gravidité négatif au 40è jour). Par ailleurs, la croissance foetale a été suivie chez 16 femelles gravides du 45è au 120è jour postinsémination. Les principales mensurations foetales réalisées ont été les diamètres du crâne et du tronc. Au 45è jour de la gravidité, les diamètres du crâne et du tronc ont été respectivement de 1,8 ± 0,1 cm et 1,4 ± 0,3 cm. Au 120è jour, ces valeurs ont été de 6,5 ± 0,6 cm et 5,8 ± 0,5 cm. A partir du 120è jour, l'utérus a pris une position très profonde, ne permettant plus la visualisation du foetus en croissance. [less ▲]

Isolation and partial characterization of three pregnancy-associated glycoproteins from the ewe placenta

in Molecular Reproduction and Development (2003), 64(2), 199-206

Pregnancy-associated glycoproteins (PAGs) are synthesized in the outer epithelial layer of the placenta in artiodactyls. In this work, three novel ovine PAGs were isolated from late-pregnancy fetal ... [more ▼]

Pregnancy-associated glycoproteins (PAGs) are synthesized in the outer epithelial layer of the placenta in artiodactyls. In this work, three novel ovine PAGs were isolated from late-pregnancy fetal cotyledons and characterized biochemically. The isolation procedure included acid and ammonium sulfate precipitations and anion and cation exchange chromatographies. The isolated PAGs have different NH2-terminal amino acid sequences (RGSXLTILPLRNMRDIVY, ISRVSXLTIHPLRNIMDML, and RGSNLTIHPLRNIRD) and apparent molecular masses (55, 57, and 59 kDa). Each shows several isoforms with different pl values. The three proteins share high sequence identity with each other and with other ovine, bovine, and caprine PAGs. They have not been described previously. The ovPAG-59 sequence differs from the previously identified ovPAG-4 sequence (determined by DNA cloning and sequencing) at only one position among the 15 N-terminal residues. The newly characterized ovPAGs and the procedure used to isolate them will be helpful in producing new antisera for investigating PAG secretion in pregnant ewes. [less ▲]

Signaux embryonnaires de la gestation

in Journées Européennes de la Société Française de Buiatrie (2002, October 29)

Entre l’endomètre et le trophectoderme, les interactions complexes qui aboutissent à l’implantation et à la mise en place du placenta sont harmonisées par le conceptus qui envoie des signaux auxquels ... [more ▼]

Entre l’endomètre et le trophectoderme, les interactions complexes qui aboutissent à l’implantation et à la mise en place du placenta sont harmonisées par le conceptus qui envoie des signaux auxquels l’utérus répond localement ou qui contribuent à modifier l’endocrinologie maternelle (Thibault et al, 1998). Les principaux signaux embryonnaires associés à l’établissement de la gestation seront passés en revue sur base des connaissances actuelles disponibles dans la littérature scientifique. [less ▲]

Purificacao, caracterizacao e dosagem radioimunologica de glicoproteinas associadas a gestacao em zebuinos

Doctoral thesis (2002)

The pregnancy-associated glycoproteins (PAGs) constitute a large family of aspartic proteinases expressed in the outer epithelial cell layer of the Artyodactyla placenta. In the first part of the present ... [more ▼]

The pregnancy-associated glycoproteins (PAGs) constitute a large family of aspartic proteinases expressed in the outer epithelial cell layer of the Artyodactyla placenta. In the first part of the present work, two biochemical approaches were used to characterize PAGs isolated from zebu (Bos indicus) fetal cotyledons. The first procedure, used to isolate PAG from zebu placenta removed late in pregnancy, included extraction of proteins at neutral pH, acidic and ammonium sulfate precipitations, anion and cation exchange chromatographies. The second procedure, used to investigate PAG in placentas removed at early and mid gestational periods, included protein extractions at acid or alkaline pH followed by pepstatin-A agarose affinity chromatography. A bovine PAG radioimmunoassay was used to monitor the immunoreactivity throughout the isolation procedures. The most immunoreactive fractions issued from cation exchange and affinity chromatographies were analyzed by SDS-PAGE and Western Blot, before transfer to polyvinylidene difluoride (PVDF) membrane for NH2-microsequence determination. By use of SDS-PAGE and Western Blot, different isoforms of PAG with apparent molecular masses from 51 to 69 kDa and isoeletric points varying from 3.1 to 6.7 were identified in placentas from different gestational ages. After CM ceramic chromatography of all except 0.32 M NaCl DEAE fraction, the most immunoreactive proteins revealed N-terminal amino acid sequences (10 to 25 aa long) which were 100% identical to bovine PAG-1. The same sequence (14 aa long) was found after pepstatin-agarose affinity chromatography of proteins extracted from placentas removed earlier in pregnancy. These results converged towards the expression of one major N-terminal PAG amino acid sequence in zebu placentas at different gestational ages. In the second part of this study, two specific RIA systems were developed then used to measure plasmatic PAG concentrations during gestation and postpartum period in Azawak zebu cows. Twelve females palpated per rectum and diagnosed as pregnant were bled at 5-10 days interval approximately from Week 8 of gestation till Week 10 postpartum (pp). One zebu cow initially diagnosed as pregnant showed PAG concentrations lower than the assay sensitivity (<0.20 ng/ml) and did not calve. Another cow showed abnormally high PAG concentrations during gestation, being excluded from the general PAG profile. The 10 other zebu cows gave a very homogeneous PAG profile. In these animals, concentrations increased progressively from Week 8 to Week 35 of gestation (from 6.04.2 to 196.034.8 ng/ml), remaining relatively constant until Week 39 (210.874.8 ng/ml), when they increased sharply to reach their highest level (1,095.6607.2 ng/ml) at parturition. After delivery, PAG concentrations declined significantly (P<0.05) till the Week 2 postpartum (348.4  85.6 ng/ml) and slowly till the Week 10 postpartum. These results revealed that PAG concentrations in zebu cattle were lower than those previously described in taurine breeds between week 35 and 39 of gestation. [less ▲]