References of "Melin, Pierrette"
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See detailEUCAST: follow-up. What's new? What will happen in 2009
Melin, Pierrette ULg

Conference (2008, November 06)

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See detailDistribution of serotypes of clinical group B streptococci isolated in Belgium: a decade review
MELIN, Pierrette ULg; De Mol, Patrick ULg

in LISSSD Board (Ed.) Abstract book (2008, June)

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See detailEUCAST - Introduction of new breakpoints in the labs: Challenges and Solutions
Melin, Pierrette ULg

Conference (2008, May 26)

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See detailIntroduction of new breakpoints in the labs: Challenges and Solutions
Melin, Pierrette ULg

Conference (2008, May 26)

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See detailEvaluation of the StrepB Select agar for the detection of group B streptococci from vaginal and recto-vaginal specimens
MELIN, Pierrette ULg; HUYNEN, Pascale ULg; MEEX, Cécile ULg et al

in ESCMID (Ed.) Program and Abstracts book of the 18th ECCMID (2008, April)

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See detailPresenec of extended-spectrum beta-lactamase-producing Enterobacteriaceae in the fecal flora of patients from general practice
MEEX, Cécile ULg; MELIN, Pierrette ULg; Docquier, J. D. et al

in ECCMID (Ed.) Program and Abstracts book of the 18th ECCMID (2008, April)

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See detailThe Sanford Guide To Antimicrobial Therapy - 21st edition of the Belgian/Luxembourg Version 2008-2009
Independent Belgian/Luxembourg Working Party on Antimicrobial Therapy; MELIN, Pierrette ULg

Book published by SBIMC-BVIKM - Adapted for use by the medical profession in Belium and Luxembourg by the Independent Belgian/Luxembourg Working Party on Antimicrobial Therapy (2008)

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See detailGroup B Streptococcal Disease in the Newborn: Maternal GBS-Screening Methods and Antimicrobial Prophylaxis
Melin, Pierrette ULg

in European Obstetrics and Gynaecology - Touch Briefings (2008), 3

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See detailDecontamination of Resistant Emerging Pathogens
Melin, Pierrette ULg

Conference (2007, December 11)

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See detailPrevention of GBS perinatal infections: guidelines and update
Melin, Pierrette ULg

Conference (2007, September 27)

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See detailSusceptibility profile to penicillin, erythromycin and clindamycin of clinical isolates of grup B streptococci recently isolated in Belgium and detection of erythromycin resistance genes
MELIN, Pierrette ULg; Megali, Caroline; Hayette, Marie-Pierre et al

in ASM (Ed.) Program and Abstracts of the 47th Intersciences Conference on Antimicrobial Agents and Chemotherapy (2007, September)

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See detailLiaison VZV IgG and VZV IgM assays: a comparative study
Huynen, Pascale ULg; Melin, Pierrette ULg; De Mol, Patrick ULg

Poster (2007, September)

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See detailThe microbial diagnosis of infective endocarditis (IE)
Melin, Pierrette ULg

Scientific conference (2007, June 19)

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See detailInfective endocarditis- Microbiological Diagnosis: Efficiency and usefulness
Melin, Pierrette ULg

Conference (2007, May 10)

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See detailLIAISON® VZV IgG and VZV IgM assays: a comparative study
HUYNEN, Pascale ULg; MELIN, Pierrette ULg; HAYETTE, Marie-Pierre ULg et al

in Clinical Microbiology & Infection (2007, April), 13(Suppl. S1), 639

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See detailEvaluation of a new commercial real time PCR for the detection of Aspergillus spp. in serum and respiratory samples
Hayette, Marie-Pierre ULg; Meex, Cécile ULg; Boreux, Raphaël ULg et al

Poster (2007, April)

Objectives. Diagnosis of invasive aspergillosis is still disappointing and often delayed because of the lack of sensitivity of diagnostic tools. DNA detection based-methods have been developed, but differ ... [more ▼]

Objectives. Diagnosis of invasive aspergillosis is still disappointing and often delayed because of the lack of sensitivity of diagnostic tools. DNA detection based-methods have been developed, but differ widely and comparisons are difficult to assess. The objective of the study is to compare a new commercial real-time PCR kit, affigene® Aspergillus tracer assay, with an in house nested PCR targeting 18S rRNA Aspergillus sp. gene. Methods. Twelve patients at risk for invasive aspergillosis were included in the study. They were classified to have possible (5 cases), probable (1 case) or proven (6 cases) invasive aspergillosis following E.O.R.T.C. criteria. Fifteen serum and respiratory paired samples were collected. The DNA extraction was performed by using the QIAmp DNA mini kit® (Qiagen, Germany). All samples were tested by both PCR assays and respiratory samples were cultured. Results. Respiratory samples. A. fumigatus, A. niger and A. flavus were isolated from 10/15 samples; both PCR methods were positive for these samples except one that was positive for affigene® and equivocal for the nested PCR. The real-time PCR assay reported cycle thresholds ranging from 25 to 38. Three of the five culture-negative samples were negative by both PCR methods; one of three was negative in affigene® assay and equivocal by nested PCR; the last sample was positive in affigene® assay and negative by nested PCR. Serum. Thirteen of fifteen blood samples were negative by both PCR methods. One sample was equivocal by nested PCR and was inhibited in affigene® assay despite a culture-positive paired respiratory sample. The last case was inhibited by the real-time PCR assay and negative by nested PCR. Nor the nested PCR, nor affigene® assay could detect any Aspergillus DNA in serum. In total, there was 93% of agreement between the two PCR assays. Conclusion. Both methods are in good agreement and can detect at least three different species of Aspergillus. However, the sensitivity of both assays does not permit the detection of Aspergillus DNA in serum. affigene® assay can easy replace the “in house” assay: it allows a fast and standardized detection of Aspergillus sp. DNA in respiratory samples without inconvenient due to the handling of PCR products. [less ▲]

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See detailThird Belgian multicentre survey of antibiotic susceptibility of anaerobic bacteria
Wybo, Ingrid; Piérard, Denis ULg; Verschraegen, Inge et al

in Journal of Antimicrobial Chemotherapy (2007), 59(1), 132-139

Objectives: To collect recent data on the susceptibility of anaerobes and to compare them with results from previous studies. Methods: Four hundred and forty-three anaerobic clinical isolates from various ... [more ▼]

Objectives: To collect recent data on the susceptibility of anaerobes and to compare them with results from previous studies. Methods: Four hundred and forty-three anaerobic clinical isolates from various body sites were prospectively collected from October 2003 to February 2005 in nine Belgian hospitals. MICs were determined for nine anti-anaerobic and three recently developed antibiotics. Results: Most Gram-negative bacilli except Fusobacterium spp. were resistant to penicillin. Piperacillin/tazobactam, metronidazole, chloramphenicol, meropenem and amoxicillin/clavulanic acid were very active against all groups, but only 86% of Bacteroides fragilis group strains were susceptible to the latter. Cefoxitin, cefotetan and clindamycin were less active. In particular, only 62%, 52% and 48% of B. fragilis group strains were susceptible, respectively. Clindamycin shows a continuing decrease in activity, as 83% were still susceptible in 1987 and 66% in 1993-94. Anti-anaerobic activity of the new antibiotics is interesting, with MIC50 and MIC90 of 1 and > 32 mg/L for moxifloxacin, 2 and 4 mg/L for linezolid and 0.5 and 8 mg/L for tigecycline. Conclusions: The susceptibility of anaerobic bacteria remains stable in Belgium, except for clindamycin, which shows a continuous decrease in activity. However, for each of the tested antibiotics, at least a few resistant organisms were detected. Consequently, for severe infections involving anaerobic bacteria, it could be advisable to perform microbiological testing instead of relying on known susceptibility profiles. Periodically monitoring background susceptibility remains necessary to guide empirical therapy. [less ▲]

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