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See detailPrevention of GBS perinatal infections: guidelines and update
Melin, Pierrette ULg

Conference (2007, September 27)

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See detailSusceptibility profile to penicillin, erythromycin and clindamycin of clinical isolates of grup B streptococci recently isolated in Belgium and detection of erythromycin resistance genes
MELIN, Pierrette ULg; Megali, Caroline; Hayette, Marie-Pierre et al

in ASM (Ed.) Program and Abstracts of the 47th Intersciences Conference on Antimicrobial Agents and Chemotherapy (2007, September)

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See detailLiaison VZV IgG and VZV IgM assays: a comparative study
Huynen, Pascale ULg; Melin, Pierrette ULg; De Mol, Patrick ULg

Poster (2007, September)

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See detailThe microbial diagnosis of infective endocarditis (IE)
Melin, Pierrette ULg

Scientific conference (2007, June 19)

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See detailInfective endocarditis- Microbiological Diagnosis: Efficiency and usefulness
Melin, Pierrette ULg

Conference (2007, May 10)

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See detailLIAISON® VZV IgG and VZV IgM assays: a comparative study
HUYNEN, Pascale ULg; MELIN, Pierrette ULg; HAYETTE, Marie-Pierre ULg et al

in Clinical Microbiology & Infection (2007, April), 13(Suppl. S1), 639

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See detailEvaluation of a new commercial real time PCR for the detection of Aspergillus spp. in serum and respiratory samples
Hayette, Marie-Pierre ULg; Meex, Cécile ULg; Boreux, Raphaël ULg et al

Poster (2007, April)

Objectives. Diagnosis of invasive aspergillosis is still disappointing and often delayed because of the lack of sensitivity of diagnostic tools. DNA detection based-methods have been developed, but differ ... [more ▼]

Objectives. Diagnosis of invasive aspergillosis is still disappointing and often delayed because of the lack of sensitivity of diagnostic tools. DNA detection based-methods have been developed, but differ widely and comparisons are difficult to assess. The objective of the study is to compare a new commercial real-time PCR kit, affigene® Aspergillus tracer assay, with an in house nested PCR targeting 18S rRNA Aspergillus sp. gene. Methods. Twelve patients at risk for invasive aspergillosis were included in the study. They were classified to have possible (5 cases), probable (1 case) or proven (6 cases) invasive aspergillosis following E.O.R.T.C. criteria. Fifteen serum and respiratory paired samples were collected. The DNA extraction was performed by using the QIAmp DNA mini kit® (Qiagen, Germany). All samples were tested by both PCR assays and respiratory samples were cultured. Results. Respiratory samples. A. fumigatus, A. niger and A. flavus were isolated from 10/15 samples; both PCR methods were positive for these samples except one that was positive for affigene® and equivocal for the nested PCR. The real-time PCR assay reported cycle thresholds ranging from 25 to 38. Three of the five culture-negative samples were negative by both PCR methods; one of three was negative in affigene® assay and equivocal by nested PCR; the last sample was positive in affigene® assay and negative by nested PCR. Serum. Thirteen of fifteen blood samples were negative by both PCR methods. One sample was equivocal by nested PCR and was inhibited in affigene® assay despite a culture-positive paired respiratory sample. The last case was inhibited by the real-time PCR assay and negative by nested PCR. Nor the nested PCR, nor affigene® assay could detect any Aspergillus DNA in serum. In total, there was 93% of agreement between the two PCR assays. Conclusion. Both methods are in good agreement and can detect at least three different species of Aspergillus. However, the sensitivity of both assays does not permit the detection of Aspergillus DNA in serum. affigene® assay can easy replace the “in house” assay: it allows a fast and standardized detection of Aspergillus sp. DNA in respiratory samples without inconvenient due to the handling of PCR products. [less ▲]

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See detailThird Belgian multicentre survey of antibiotic susceptibility of anaerobic bacteria
Wybo, Ingrid; Piérard, Denis ULg; Verschraegen, Inge et al

in Journal of Antimicrobial Chemotherapy (2007), 59(1), 132-139

Objectives: To collect recent data on the susceptibility of anaerobes and to compare them with results from previous studies. Methods: Four hundred and forty-three anaerobic clinical isolates from various ... [more ▼]

Objectives: To collect recent data on the susceptibility of anaerobes and to compare them with results from previous studies. Methods: Four hundred and forty-three anaerobic clinical isolates from various body sites were prospectively collected from October 2003 to February 2005 in nine Belgian hospitals. MICs were determined for nine anti-anaerobic and three recently developed antibiotics. Results: Most Gram-negative bacilli except Fusobacterium spp. were resistant to penicillin. Piperacillin/tazobactam, metronidazole, chloramphenicol, meropenem and amoxicillin/clavulanic acid were very active against all groups, but only 86% of Bacteroides fragilis group strains were susceptible to the latter. Cefoxitin, cefotetan and clindamycin were less active. In particular, only 62%, 52% and 48% of B. fragilis group strains were susceptible, respectively. Clindamycin shows a continuing decrease in activity, as 83% were still susceptible in 1987 and 66% in 1993-94. Anti-anaerobic activity of the new antibiotics is interesting, with MIC50 and MIC90 of 1 and > 32 mg/L for moxifloxacin, 2 and 4 mg/L for linezolid and 0.5 and 8 mg/L for tigecycline. Conclusions: The susceptibility of anaerobic bacteria remains stable in Belgium, except for clindamycin, which shows a continuous decrease in activity. However, for each of the tested antibiotics, at least a few resistant organisms were detected. Consequently, for severe infections involving anaerobic bacteria, it could be advisable to perform microbiological testing instead of relying on known susceptibility profiles. Periodically monitoring background susceptibility remains necessary to guide empirical therapy. [less ▲]

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See detailPrevention of Perinatal Group B Streptococcal Diseases: Belgian Guidelines
Melin, Pierrette ULg

in Round Table Series (2007), 85

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See detailDisseminated Penicillium marneffei infection contrated in China
Hayette, Marie-Pierre ULg; Mukeba Tschialala, Didier; Meex, Cécile ULg et al

Poster (2007)

Penicillium marneffei infection is a rare fungal disease that cause significant disease in immunosuppressed patients. The geographical distribution of this dimorphic fungus is restricted to Asia ... [more ▼]

Penicillium marneffei infection is a rare fungal disease that cause significant disease in immunosuppressed patients. The geographical distribution of this dimorphic fungus is restricted to Asia, Southeast and Far East, where the disease is considered as an indicator of acquired immunodeficiency syndrome (AIDS). Case report. A 42-year old Congolese woman living in Lubumbashi was admitted at the university hospital of Liège for exploration of a general status impairment. She experienced for three months spiking fever, weight loss, productive cough with bloody expectorations and progressive dyspnoea. She reported also to have non-bloody mild diarrhoea with abdominal pain. The HIV antibody status was positive with a low CD4 T lymphocytes count (28/µl). Pulmonary infiltrates were visualized on chest radiography and the computed tomography revealed the presence of a severe pneumopathy characterised by bilateral micronodular lesions. Mediastinal polyadenopathies associated with hepato- and splenomegaly were also highlighted. Bronchoscopy was performed and bronchial aspirations revealed the presence of numerous leucocytes with the presence of intracellular Gram positive organisms suggestive of yeasts. Ziehl, Giemsa and Gomori-Grocott staining were also performed. Ziehl staining was negative. The morphological aspect given by Giemsa staining excluded infection and the PCR specific for T. gondii B1 gene was negative. However, Gomori-Grocott staining revealed the presence of intracellular oval, elongated, sausage-shaped cells with a single transverse septum (3 to 5 µm). Penicillium marneffei was isolated from blood culture and respiratory samples. Intraveinous amphotericin B treatment was administrated during 15 days followed by itraconazole oral administration (200 mg/j). The antimycotic treatment improved the patient condition and despite other clinical troubles she was prematurely discharged because of financial problems. Conclusion. Opportunistic agents involved in HIV-infected patients differ in Africa and Asia and it is important to be able to make a rapid diagnosis with the aid of an experienced laboratory. [less ▲]

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See detailStreptococcus agalactiae (dans rapport de surveillance des maladies infectieuses par un réseau de laboratoires de microbilogie 2005 + Tendances épidémiologiques 1983-2004)
MELIN, Pierrette ULg

in Ducoffre, Geneviève (Ed.) Rapport de surveillance des maladies infectieuses par un réseau de laboratoires de microbiologie 2005 + Tendances épidémiologiques 1983-2004 - IPH/EPI REPORT 2005 (2006)

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See detailPrévention des infections périnatales à streptocoques du groupe B : Recommandations
MELIN, Pierrette ULg

Conference (2006, September 08)

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See detailEarly onset neonatal sepsis or meningitis, in Belgium, in the era of prevention for perinatal group B streptococcal diseases
MELIN, Pierrette ULg; Hayette, Marie-Pierre; Ducoffre, Geneviève et al

in ASM (Ed.) Program and Abstracts of the 46th Intersciences Conference on Antimicrobial Agents and Chemotherapy (2006, September)

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See detailMolecular epidemiology of extended-spectrum beta-lactamase-producing Enterobacteriaceae colonizing the digestive tract of patients admitted to intensive care units in a Belgian university hospital
CHRISTIAENS, Geneviève ULg; Damas, Pierre ULg; Docquier, J. D. et al

in American Society of Microbiology (Ed.) Program and Abstracts of the 46th Intersciences Conference on Antimicrobial Agents and Chemotherapy (2006, September)

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See detailThe GBS: pro screening
MELIN, Pierrette ULg

Conference (2006, June 21)

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See detailEvaluation of the Strepto B ID agar for the detection of group B streptococci from vaginal and recto-vaginal specimens
MELIN, Pierrette ULg; Bonafe, S.; HAYETTE, Marie-Pierre ULg et al

in ASM (Ed.) Program and Abstracts of the 106th General Meeting of the American Society for Microbiology (2006, May)

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See detailThe GBS: pro screening
MELIN, Pierrette ULg

Conference (2006, March 11)

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