Grafting cultured macrophages or fibroblasts in a contusion model of adult rat spinal cord : a combined morphological and biochemical study of neuronal and non-neuronal actors in repair processes.
Franzen, Rachelle ; Martin, Didier ; et al
Conference (1997)Detailed reference viewed: 4 (3 ULg)
Current concepts in the prevention, diagnosis and treatment of the cervical whiplash syndrome : Acute cervical central cord syndrome.
Conference (1996, November 15)Detailed reference viewed: 5 (1 ULg)
Nt-3 Has a Tropic Effect on Process Outgrowth by Postnatal Auditory Neurones in Vitro
Malgrange, Brigitte ; ; Martin, Didier et al
in Neuroreport (1996), 7(15-17), 2495-9
CONFOCAL analysis of early postnatal auditory neurones in a bicompartmental culture system was used to test for chemoattractant properties of NGF, BDNF and NT-3 on neuronal process outgrowth. NT-3 exerted ... [more ▼]
CONFOCAL analysis of early postnatal auditory neurones in a bicompartmental culture system was used to test for chemoattractant properties of NGF, BDNF and NT-3 on neuronal process outgrowth. NT-3 exerted a strong tropic effect on neuritic outgrowth from auditory neurones in this system. BDNF and NGF did not have any tropic activity that directed processes outgrowth from auditory neurones. However, BDNF was important for the support of neuronal survival in NGF-treated cultures and for neuritogenesis in NT-3-treated cultures. Since NT-3 has been identified as both a survival factor and a chemotropic agent for auditory neurones, it is likely that this neurotrophin will be a useful therapeutic agent in the treatment of damaged cochleae for the recovery of hearing. [less ▲]Detailed reference viewed: 22 (0 ULg)
Intraarterial spiral CT angiography combined with DSA in pretherapeutic assessment of cerebral aneurysms.
; ; Dondelinger, Robert et al
Conference (1996, September 21)Detailed reference viewed: 12 (0 ULg)
Effects of Schwann Cell Transplantation in a Contusion Model of Rat Spinal Cord Injury
Martin, Didier ; Robe, Pierre ; Franzen, Rachelle et al
in Journal of Neuroscience Research (1996), 45(5), 588-597
Cultured Schwann cells were transplanted at various delays into a spinal cord contusion injury performed at low thoracic level in adult female rats. The Schwann cells were purified from the dorsal root ... [more ▼]
Cultured Schwann cells were transplanted at various delays into a spinal cord contusion injury performed at low thoracic level in adult female rats. The Schwann cells were purified from the dorsal root ganglia of adult syngeneic animals. the transplants were well tolerated, and the transplanted Schwann cells invaded the injured spinal cord. As quantified using video image analysis, the survival and growth of the transplanted cells were poor when the grafting procedure was performed 3-4 days after injury and very good when performed immediately or 10 days after injury, in which cases post-traumatic micro- and macrocavitation were strongly reduced. In animals grafted immediately after injury but not in animals grafted after 10 days, post-traumatic astrogliosis was much reduced. The Schwann cells transplanted area was invaded by numerous regenerating axons, the vast majority of which were, based on the neurotransmitter (CGRP and SP) profile, originating from dorsal root ganglion. No regeneration of the corticospinal tract as assessed after anterograde tracing or of descending aminergic fibers could be demonstrated. [less ▲]Detailed reference viewed: 38 (5 ULg)
Exérèse sous contrôle vidéoscopique de neurinomes intrathoracique: réflexions à propos de trois cas.
Lenelle, Jacques ; Martin, Didier ; et al
Conference (1996, June 16)Detailed reference viewed: 14 (1 ULg)
Pretherapeutic assessment of intracranial aneurysms with intra-arterially enhanced 3-D CT-angiography.
Martin, Didier ; Lenelle, Jacques ; et al
Conference (1996, April 27)Detailed reference viewed: 1 (0 ULg)
Migration épidurale postérieure d'une hernie discale lombaire : a propos de deux cas.
Robe, Pierre ; Martin, Didier ; Lenelle, Jacques et al
Conference (1996, March 16)Detailed reference viewed: 32 (2 ULg)
Les hernies discales dorsales : a propos de 7 cas opérés
Dubuisson, Annie ; Lenelle, Jacques ; Martin, Didier et al
Conference (1996, March 16)Detailed reference viewed: 96 (3 ULg)
Les lésions de la moelle épinière : du matériel expérimental aux transplantations médullaires
Conference (1996, February 15)Detailed reference viewed: 5 (1 ULg)
Surface modification of biomaterials in order to control cell adhesion.
; ; et al
Conference (1996)Detailed reference viewed: 10 (3 ULg)
Alpha-MSH stimulates neurite outgrowth of neonatal rat corticospinal neurons in vitro.
; ; Martin, Didier et al
in Brain Research (1996), 736(1-2), 91-8
Peptides related to melanocortin (alpha MSH) and corticotropin (ACTH), collectively termed melanocortins, exert trophic effects on the outgrowth of neurites from peripheral and central nervous system in ... [more ▼]
Peptides related to melanocortin (alpha MSH) and corticotropin (ACTH), collectively termed melanocortins, exert trophic effects on the outgrowth of neurites from peripheral and central nervous system in vitro. Here we study the neurite outgrowth promoting effect of alpha-MSH on corticospinal (CS) neurons in vitro. Corticospinal neurons were identified in cell culture of neonatal rat cortex by immunostaining of cholera toxin subunit B (CTB), retrogradely transported from the cervical parts of the spinal cord. The CTB-immunoreactive neurons represent a small percentage (3-5%) of the total cell population after 72 h in vitro. The axons or dendrites of cortical and CTB-labelled layer V neurons were visualized using antibodies against axon- or dendrite-specific markers and measured using a semi-automatic quantification device. Here we report that alpha-MSH stimulates axonal as well as dendrite outgrowth from both total and CTB-labelled neurons with a bell-shape response curve. Axonal outgrowth of CTB-labelled neurons was dose-dependently stimulated with a maximal effect of 50% at 10(-10) M alpha-MSH. The maximal effect for stimulation of axon outgrowth for the total cortex population was observed at 10(-8) M alpha-MSH. In addition dendrite outgrowth of both total and CTB-labelled neurons is stimulated in a dose-dependent manner with maximal effects (varying between 46 and 48%) at 10(-8) M alpha-MSH. Explanations in the shift for the optimal alpha-MSH concentration for stimulation of axonal outgrowth of CTB-labelled layer V neurons as compared to total cortex neurons are discussed. [less ▲]Detailed reference viewed: 38 (0 ULg)
Comparison of brain temperature with tympanic membrane, esophagal and rectal temperatures in neurosurgical patients.
Franssen, Colette ; ; et al
Conference (1996)Detailed reference viewed: 9 (0 ULg)
Pretherapeutic assessment of cerebral aneuvrysms with arterially enhanced spiral CT
; ; Dondelinger, Robert et al
Conference (1995, November 26)Detailed reference viewed: 3 (0 ULg)
Preparation of a Macroporous Biodegradable Polylactide Implant for Neuronal Transplantation
; Grandfils, Christian ; Jérôme, Robert et al
in Journal of Biomedical Materials Research (1995), 29(11), 1349-62
This article reports the production of a surgical implant meeting several specific requirements such as biocompatibility, biodegradability, macroporosity, and flexibility. Porosity was controlled by an ... [more ▼]
This article reports the production of a surgical implant meeting several specific requirements such as biocompatibility, biodegradability, macroporosity, and flexibility. Porosity was controlled by an original method consisting of the aggregation of calibrated poly-D,L-lactide microparticles. The size of the interstices between the aggregated microspheres was in a direct relationship to the microsphere diameter. A first approach was based on coating the microspheres with poly(vinyl alcohol) followed by chemically crosslinking the coating layers that were in mutual contact. This method was disregarded because of the acute cytotoxicity of glutaraldehyde used as the crosslinking agent, the absence of macroporosity, and the complete lack of flexibility. A physical technique of aggregation was then tested, which relied on the plasticization of poly-D,L-lactide microspheres with triethylcitrate to the point where microspheres strongly adhered to each other when they were in contact. This method has proved to be straightforward and definitely superior to the chemical approach, particularly with respect to cytotoxicity, control of macroporosity, and flexibility. A polymer support was thus successfully which was biodegradable, macroporous( interconnected pores of 10-100 microns in diameter), and flexible. This potential medical device is presently being used for neuronal transplantation in the central nervous system. [less ▲]Detailed reference viewed: 31 (5 ULg)
Quantitative assessment by western-blot of proteins expressed by different cell types involved in inhibition and promotion of regeneration in the lesioned spinal cord.
; Martin, Didier ; Leprince, Pierre et al
Conference (1995, September 21)Detailed reference viewed: 6 (0 ULg)