References of "Marlier, Didier"
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See detailControl of Salmonella contamination of shell eggs – preharvest and postharvest methods: a review
Galis, Anca; Marcq, Christopher ULg; Marlier, Didier ULg et al

in Comprehensive Reviews in Food Science and Food Safety (2013), 12

Salmonella Enteritidis is one of the most prevalence foodborne pathogen, its most important reservoir being considered the shell egg. As the concerns related to the increasing human salmonellosis cases ... [more ▼]

Salmonella Enteritidis is one of the most prevalence foodborne pathogen, its most important reservoir being considered the shell egg. As the concerns related to the increasing human salmonellosis cases grow, the need for an application of preventive methods either at the farm level or during the processing steps is crucial for a better control of the foodborne outbreaks due to the consumption of this specific food product. This review focuses on the application of preventive methods at the farm level, on preharvest step, in order to reduce the risk of shell eggs contamination with Salmonella, especially S. Enteritidis, through a better control of the laying hens’ infection with this pathogen. As postharvest methods, a first approach is the egg storage conditions and the prevention of Salmonella spp. growth and multiplication. In addition, shell eggs may be subjected to eggshell decontamination, to reduce the risk of foodborne outbreaks. Several of these latter mentioned methods are already authorized to be put in place in different countries, as it is the case in the United States of America and Canada. Their efficacy has been proven and their use is regarded by some as mandatory for ensuring shell eggs safety for the consumers. [less ▲]

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See detailComparison of corticosterone, cortisol, triglycerides, aspartateaminotransferase and uric acid plasma concentrations during a foie gras production period in male mule ducks (Anas platyrhynchos X Cairina moschata)
Flament, Aline; Delleur, Valéry ULg; Poulipoulis, Anastassios ULg et al

in British Poultry Science (2012), 53(4), 408-414

1 Corticosterone, cortisol, triglycerides, aspartate aminotransferase (AST) and uric acid (UA) plasma concentration were measured at 8 and 12 weeks of age, at slaughtering and 45 min after an ... [more ▼]

1 Corticosterone, cortisol, triglycerides, aspartate aminotransferase (AST) and uric acid (UA) plasma concentration were measured at 8 and 12 weeks of age, at slaughtering and 45 min after an adrenocorticotrophic hormone (ACTH) stimulation test (8 weeks of age) in twelve male mule ducks. 2 No significant increase of corticosterone was found during the force feeding period. 3 Comparison of corticosterone and cortisol levels indicates that cortisol can be considered as a reliable acute stress indicator in future routine examinations. 4 Triglycerides and AST plasma concentrations progressively increased from pre-force feeding period to slaughtering. 5 UA plasma concentrations also increased from the start at 8 weeks of age to mid-force feeding time but no difference was noticed between mid-force feeding period and slaughtering. [less ▲]

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See detailGastrointestinal diseases
Marlier, Didier ULg

Scientific conference (2012, February 11)

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See detailContraintes techniques et sanitaires de la production traditionnelle de pintade en Afrique subsaharienne
Boko, C; Kpodekon, T; Dahouda, M et al

in Annales de Médecine Vétérinaire (2012), 156

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See detailPrevalence of Salmonella serotypes in male mule ducks in Belgium
Flament, Aline; Soubbotina, Alexandra; Mainil, Jacques ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2012)

Salmonella species infections of male mule ducks were studied for 32 months in 100 flocks on nine duck farms in Belgium. The prevalence of Salmonella species infections changed significantly over time (P ... [more ▼]

Salmonella species infections of male mule ducks were studied for 32 months in 100 flocks on nine duck farms in Belgium. The prevalence of Salmonella species infections changed significantly over time (P<0.001) with infection rates of 50, 13.4, 6.7, 2.6 and 2.9 per cent, respectively, at the time of arrival on the farm, at three, six and nine weeks of age, and when the ducks left the breeding unit to enter the force-feeding rooms (at 11 or 12 weeks of age). During the study period, 95 strains of Salmonella were isolated, belonging to 11 serotypes. S Indiana (42.1 per cent) and S Regent (36.8 per cent) were the two most common serotypes, whereas S Typhimurium and S Enteritidis were found only once (1.1 per cent). All isolated strains were resistant to at least two antimicrobials, but resistance to more than five antimicrobials was observed in 21.6 per cent of the strains. [less ▲]

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See detailPneumonia with Aeromonas sobria in a Carpet Python
Gandar, Frederic ULg; Szalo, Ioan Mihai ULg; Marlier, Didier ULg

Conference (2011, August 11)

Aeromonas sobria was isolated and identified upon post-mortem examination from the respiratory tract and the blood of a carpet python (Morelia spilota variegata). The snake was referred to the Faculty of ... [more ▼]

Aeromonas sobria was isolated and identified upon post-mortem examination from the respiratory tract and the blood of a carpet python (Morelia spilota variegata). The snake was referred to the Faculty of Veterinary Medicine of Liège for necropsy, just the day after it suddenly died without previous clinical sign. Lung and liver biopsies were performed and fixed in neutral buffered 10% formalin and paraffin embedded. Blood samples were collected via cardiocentesis, and air sac abscesses were cultured. Bacterial strains were identified as Aeromonas sobria by 16S rDNA sequencing. Based on histological and bacterial examinations, the death of this snake was attributed to a septicemia, following an acute primary, or secondary exudative pneumonia. Aeromonas sp. is established as a potential pathogen in reptiles. Among this genus, Aeromonas hydrophila is the most frequently isolated. A. sobria has been reported as a primary pathogen in farmed perch (Perca fluviatilis) and humans. Conversely, few data are available concerning the pathogenicity of A. sobria in reptiles. Other non-bacterial agents (virus, fungus, endoparasites) or predisposing factors (such as obesity) can also be responsible for respiratory tract disease in snakes . Unfortunately, in the current case, virological investigations were not performed. [less ▲]

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See detailEpizootic rabbit enteropathy: Comparison of PCR-based RAPD fingerprints from virulent and non-virulent samples
Huybens, Nathalie ULg; Houeix, Julien ULg; Licois, Dominique et al

in Veterinary Journal (2011), 190(3), 416-417

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See detailEpizootic rabbit enteropathy inoculum (TEC4) antibiograms and antibiotic fractionation
Huybens, Nathalie ULg; Houeix, Julien ULg; Licois, Dominique et al

in Veterinary Research Communications (2011), 35

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See detailVaccination strategies against myxomavius infections: are we really doing the best?
Marlier, Didier ULg

in Tijdschrift voor Diergeneeskunde (2010), 134

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See detailLes techniques de biologie moléculaire d'analyse des populations bactériennes complexes
Huybens, Nathalie ULg; Mainil, Jacques ULg; Marlier, Didier ULg

in Annales de Médecine Vétérinaire (2009), 153(2), 112-128

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See detailINOCULATION AND BACTERIAL ANALYSES OF FRACTIONS OBTAINED FROM THE REFERENCE INOCULUM TEC4 WHICH EXPERIMENTALLY REPRODUCES EPIZOOTIC RABBIT ENTEROPATHY
Huybens, Nathalie ULg; Houeix, Julien ULg; Licois, D. et al

in World Rabbit Science (2009), 17(4), 185-193

The aetiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial aetiology is the basis of current research. The fractionation of the ... [more ▼]

The aetiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial aetiology is the basis of current research. The fractionation of the reference inoculum (TEC4) is a major step towards finding the potential bacterial agent(s). In this study, TEC4 was fractionated by different techniques: centrifugation on discontinuous sucrose gradient, cell adherence and chloroform/ethanol treatment. The different fractions were inoculated into SPF rabbits and analyzed with classical bacteriological techniques. ERE was reproduced with two of the six fractions obtained. Four species never previously cultured from TEC were identified in the process but, to date, none of them seems to be the aetiology of ERE. [less ▲]

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See detailIS EPIZOOTIC RABBIT ENTEROPATHY (ERE) A BACTERIAL DISEASE?
Huybens, Nathalie ULg; Houeix, Julien ULg; Szalo, Ioan Mihai ULg et al

in Proceeding of the 9th World Rabbit Congress (2008, June 12)

The etiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial etiology is at the basis of current research. The fractionation of the ... [more ▼]

The etiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial etiology is at the basis of current research. The fractionation of the reference inoculum (TEC4) is a major step to find the potential bacterial agent(s). In this study, TEC4 was fractionated with two techniques: centrifugation on discontinuous sucrose gradient then cell adhesion. Two selected fractions were inoculated to SPF rabbits and analyzed with classical bacteriological techniques. ERE was reproduced with both fractions. The 16S rDNA gene was amplified in all fractions and in three negative controls and subsequently analyzed with Restriction Fragment Length Polymorphism (RFLP) and Denaturating Gradient Gel Electrophoresis (DGGE). A difference in bacterial DNA composition was found between virulent and non-virulent fractions which reinforce the potential role of bacteria in the etiology of ERE. [less ▲]

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See detailNouveautés sur l’étiologie de l’entéropathie épizootique du lapin
Huybens, Nathalie; HOUEIX, Julien ULg; Mainil, Jacques ULg et al

Conference (2008, March 04)

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See detailPathologies infectieuses du lapin en élevage rationnel
Licois, D.; Marlier, Didier ULg

in Productions Animales (2008), 21(3), 257-268

La pathologie représente un poids économique important en élevage rationnel du lapin de chair. En effet, un quart des lapereaux meurent entre la naissance et la vente. Chez les reproductrices, sur trois ... [more ▼]

La pathologie représente un poids économique important en élevage rationnel du lapin de chair. En effet, un quart des lapereaux meurent entre la naissance et la vente. Chez les reproductrices, sur trois femelles entrant dans une bande, une meurt avant la 3ème mise bas, une autre est réformée pour cause de performances insuffisantes (infertilité) ou problème sanitaire, une seule assure une production. Deux syndromes principaux sont classiquement identifiés chez le lapin : le syndrome respiratoire qui domine chez les adultes et le syndrome digestif, plus fréquent chez les lapins en croissance. Dans cette synthèse nous ferons le point sur les principales maladies infectieuses pouvant être rencontrées dans les élevages rationnels et sur les recherches développées au cours de ces dernières années concernant les agents étiologiques impliqués. [less ▲]

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See detailFractionation of the reference inoculum of epizootic rabbit enteropathy in discontinuous sucrose gradient identifies aetiological agents in high density fractions
Szalo, Ioan Mihai ULg; Lassence, Cédric ULg; Licois, Dominique et al

in Veterinary Journal (2007), 173(3), 652-657

Epizootic rabbit enteropathy (ERE) is a major cause of economic loss in intensive rabbit production. Since its first recognition in 1997, much work has been done to determine the pathogenic mechanisms of ... [more ▼]

Epizootic rabbit enteropathy (ERE) is a major cause of economic loss in intensive rabbit production. Since its first recognition in 1997, much work has been done to determine the pathogenic mechanisms of the disease and to identify the aetiological agent(s). Unfortunately, the quest for aetiology has only met with limited success despite the ability to reproduce the syndrome by inoculation of intestinal contents from field cases. These intestinal inocula contain a huge number of microorganisms which could all be involved in the aetiology of ERE. To decrease the number of putative agents, the French reference inoculum TEC3 was fractionated on a discontinuous sucrose gradient so that seven fractions (supernatant, 10%, 20%, 30%, 40%, 50% and pellet) were obtained. Specific-pathogen-free rabbits were inoculated with three out of these seven fractions (supernatant, 30%, and pellet). The objectives were: (1) to characterise the seven fractions by bacteriological examination; (2) to verify whether the aetiological agent was present in the fractions by inoculation of rabbits; (3) to assign the aetiological agent of ERE to a morphological group of pathogens; (4) to identify a fraction which could replace the reference inoculum TEC3 in applications such as cell cultures or egg inoculation. The results strongly suggest that ERE is a bacterial disease and does not have a viral or parasitic aetiology. (C) 2006 Elsevier Ltd. All rights reserved. [less ▲]

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