References of "Marlier, Didier"
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See detailVaccination strategies against myxomavius infections: are we really doing the best?
Marlier, Didier ULg

in Tijdschrift voor Diergeneeskunde (2010), 134

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See detailLes techniques de biologie moléculaire d'analyse des populations bactériennes complexes
Huybens, Nathalie ULg; Mainil, Jacques ULg; Marlier, Didier ULg

in Annales de Médecine Vétérinaire (2009), 153(2), 112-128

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See detailINOCULATION AND BACTERIAL ANALYSES OF FRACTIONS OBTAINED FROM THE REFERENCE INOCULUM TEC4 WHICH EXPERIMENTALLY REPRODUCES EPIZOOTIC RABBIT ENTEROPATHY
Huybens, Nathalie ULg; Houeix, Julien ULg; Licois, D. et al

in World Rabbit Science (2009), 17(4), 185-193

The aetiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial aetiology is the basis of current research. The fractionation of the ... [more ▼]

The aetiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial aetiology is the basis of current research. The fractionation of the reference inoculum (TEC4) is a major step towards finding the potential bacterial agent(s). In this study, TEC4 was fractionated by different techniques: centrifugation on discontinuous sucrose gradient, cell adherence and chloroform/ethanol treatment. The different fractions were inoculated into SPF rabbits and analyzed with classical bacteriological techniques. ERE was reproduced with two of the six fractions obtained. Four species never previously cultured from TEC were identified in the process but, to date, none of them seems to be the aetiology of ERE. [less ▲]

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See detailIS EPIZOOTIC RABBIT ENTEROPATHY (ERE) A BACTERIAL DISEASE?
Huybens, Nathalie ULg; Houeix, Julien ULg; Szalo, Ioan Mihai ULg et al

in Proceeding of the 9th World Rabbit Congress (2008, June 12)

The etiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial etiology is at the basis of current research. The fractionation of the ... [more ▼]

The etiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial etiology is at the basis of current research. The fractionation of the reference inoculum (TEC4) is a major step to find the potential bacterial agent(s). In this study, TEC4 was fractionated with two techniques: centrifugation on discontinuous sucrose gradient then cell adhesion. Two selected fractions were inoculated to SPF rabbits and analyzed with classical bacteriological techniques. ERE was reproduced with both fractions. The 16S rDNA gene was amplified in all fractions and in three negative controls and subsequently analyzed with Restriction Fragment Length Polymorphism (RFLP) and Denaturating Gradient Gel Electrophoresis (DGGE). A difference in bacterial DNA composition was found between virulent and non-virulent fractions which reinforce the potential role of bacteria in the etiology of ERE. [less ▲]

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See detailNouveautés sur l’étiologie de l’entéropathie épizootique du lapin
Huybens, Nathalie; HOUEIX, Julien ULg; Mainil, Jacques ULg et al

Conference (2008, March 04)

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See detailPathologies infectieuses du lapin en élevage rationnel
Licois, D.; Marlier, Didier ULg

in Productions Animales (2008), 21(3), 257-268

La pathologie représente un poids économique important en élevage rationnel du lapin de chair. En effet, un quart des lapereaux meurent entre la naissance et la vente. Chez les reproductrices, sur trois ... [more ▼]

La pathologie représente un poids économique important en élevage rationnel du lapin de chair. En effet, un quart des lapereaux meurent entre la naissance et la vente. Chez les reproductrices, sur trois femelles entrant dans une bande, une meurt avant la 3ème mise bas, une autre est réformée pour cause de performances insuffisantes (infertilité) ou problème sanitaire, une seule assure une production. Deux syndromes principaux sont classiquement identifiés chez le lapin : le syndrome respiratoire qui domine chez les adultes et le syndrome digestif, plus fréquent chez les lapins en croissance. Dans cette synthèse nous ferons le point sur les principales maladies infectieuses pouvant être rencontrées dans les élevages rationnels et sur les recherches développées au cours de ces dernières années concernant les agents étiologiques impliqués. [less ▲]

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See detailFractionation of the reference inoculum of epizootic rabbit enteropathy in discontinuous sucrose gradient identifies aetiological agents in high density fractions
Szalo, Ioan Mihai ULg; Lassence, Cédric ULg; Licois, Dominique et al

in Veterinary Journal (2007), 173(3), 652-657

Epizootic rabbit enteropathy (ERE) is a major cause of economic loss in intensive rabbit production. Since its first recognition in 1997, much work has been done to determine the pathogenic mechanisms of ... [more ▼]

Epizootic rabbit enteropathy (ERE) is a major cause of economic loss in intensive rabbit production. Since its first recognition in 1997, much work has been done to determine the pathogenic mechanisms of the disease and to identify the aetiological agent(s). Unfortunately, the quest for aetiology has only met with limited success despite the ability to reproduce the syndrome by inoculation of intestinal contents from field cases. These intestinal inocula contain a huge number of microorganisms which could all be involved in the aetiology of ERE. To decrease the number of putative agents, the French reference inoculum TEC3 was fractionated on a discontinuous sucrose gradient so that seven fractions (supernatant, 10%, 20%, 30%, 40%, 50% and pellet) were obtained. Specific-pathogen-free rabbits were inoculated with three out of these seven fractions (supernatant, 30%, and pellet). The objectives were: (1) to characterise the seven fractions by bacteriological examination; (2) to verify whether the aetiological agent was present in the fractions by inoculation of rabbits; (3) to assign the aetiological agent of ERE to a morphological group of pathogens; (4) to identify a fraction which could replace the reference inoculum TEC3 in applications such as cell cultures or egg inoculation. The results strongly suggest that ERE is a bacterial disease and does not have a viral or parasitic aetiology. (C) 2006 Elsevier Ltd. All rights reserved. [less ▲]

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See detailEpizootic Rabbit Enteropathy
Licois, D.; Coudert; Marlier, Didier ULg

in Maertens, L.; Coudert, P. (Eds.) Recent advances in rabbits sciences (2007)

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See detailExperimentally induced epizootic rabbit enteropathy: Clinical, histopathological, ultrastructural, bacteriological and haematological findings
Dewrée, Roxane; Meulemans, L.; Lassence, Cédric ULg et al

in World Rabbit Science (2007), 15(2, APR-JUN), 91-102

Epizootic rabbit enteropathy is an emerging disease that has appeared in French intensive enclosed rabbit farms since the beginning of 1997. Common clinical signs are mild watery diarrhoea with ... [more ▼]

Epizootic rabbit enteropathy is an emerging disease that has appeared in French intensive enclosed rabbit farms since the beginning of 1997. Common clinical signs are mild watery diarrhoea with considerable distension of the abdomen. At necropsy, a significant dilation of the stomach and small intestine without gross evidence of acute or chronic enteric lesions (inflammation or congestion) was observed. The purpose of this study was to describe the anatomopathologic changes concerning the small intestine and those concerning the blood profile, in experimentally infected rabbits. In a first part of the experiment, thirty animals were inoculated with a reference inoculum and five were kept as controls for clinical signs examination and histopathological study. In a second part, 17 out of the inoculated rabbits and the 5 controls animals were randomly assigned to blood testing. Microscopic lesions were studied in sections from the different parts of the small intestine (duodenum, jejunum and ileum) by light microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The histological findings revealed only limited inflammation in inoculated animals. Major villous changes were atrophy, fusion, destruction and loss of epithelial cells. In inoculated rabbits, the congestion and dilation of blood vessels of jejunal lamina propria were significantly higher than in control animals (P<0.005). There was significantly more (P<0.05) apoptosis of cells of the jejunal epithelium in inoculated rabbits than in control animals. Infiltration of polymorphonuclear neutrophiles was observed into the jejunal or ileal tunica muscularis. SEM performed on the intestinal tract of 15 inoculated rabbits revealed blankets and globular particles of mucus associated with numerous bacteria on jejunum and ileum villi. This was; not observed in the intestinal tract of control rabbits. Bacteria were found adhering to the epithelial surface and inside intestinal epithelial cells in a few animals by TEM and by light microscopy after Warthin-Starry staining. None of the bacteria isolated from the intestinal mixed contents and cultivated on usual media, are commonly known as rabbit's pathogens. Regarding the haernatological profile, neutrophil counts significantly increased (P<0.05) and lymphocyte counts significantly decreased (P<0.01), in inoculated rabbits compared to those of the control group. [less ▲]

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See detailMyxomatosis
Bertagnoli, S.; Messud-Petit, F.; Marlier, Didier ULg

in Maertens, L.; Coudert, P. (Eds.) Recent advances in rabbits sciences (2007)

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See detailIncreasing incidence of megabacteriosis in canaries (Serinus canarius domesticus)
Marlier, Didier ULg; Leroy, Cécile ULg; Sturbois, M. et al

in Veterinary Journal (2006), 172(3), 549-552

A total of 312 post-mortem examinations of 178 canaries (Serinus canarius domesticus), 40 parakeets (Melopsittacus undulatus, Nymphicus hollandicus) and 94 parrots (Amazona aestiva, Psitaccus erithacus ... [more ▼]

A total of 312 post-mortem examinations of 178 canaries (Serinus canarius domesticus), 40 parakeets (Melopsittacus undulatus, Nymphicus hollandicus) and 94 parrots (Amazona aestiva, Psitaccus erithacus) were conducted at the Birds and Rabbits Service of the University of Liege, Belgium. After a detailed gross examination, tissue samples were collected for virological and/or bacteriological and/or parasitological examination to complete the diagnosis. In all cases, a microscopic examination of the proventricular mucus layer was undertaken for the detection of the anamorphic ascomycetous yeast Macrorhabdus ornithogaster, which causes the non-zoonotic but important disease in cage birds known as megabacteriosis. At the time of death, megabacteriosis was diagnosed respectively in 28% of canaries and 22.5% of budgerigars (P value for Fisher's exact test = 0.5576), but was not diagnosed in parrots (P value for Fisher's exact test < 0.0001). The incidence of megabacteriosis significantly increases along the years (P value for chi(2) test < 0.0001, Cramer's coefficient = 0.3405). The most common gross lesions seen at necropsy of the 59 megabacteriosis cases was proventricular dilatation (86.1%). All the birds diagnosed as typical megabacteriosis cases were free of Salmonella spp. infections and of any parasitic infections. Four megabacteriosis cases (three canaries, one parakeet) were not included in statistical analysis as salmonellosis, pseudotuberculosis, coccidiosis and chlamydophilosis were diagnosed concomitantly in these birds. With the exception of megabacteriosis, the most frequent causes of death were protozoan (coccidiosis, lankesterellosis) infections (18.4%) and salmonellosis (17.1%) in canaries, and psittacosis (31.5%) and viral hepatitis (26.3%) in parakeets. In parrots, the most common causes of death were psittacosis (28.6%) and aspergillosis (28.5%). (c) 2005 Elsevier Ltd. All rights reserved. [less ▲]

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See detailDétermination des espèces bactériennes présentes dans TEC3 par analyse du polymorphisme de longueur de fragments de restriction de l’ADN codant pour l’ARN ribosomial 16S
Szalo, Mihaï; Lassence, Cédric ULg; Marlier, Didier ULg

in Proceedings 3ème colloque international de bactériologie francophone (2006, July 11)

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See detailChlamydophila psittaci chez les canards d’élevages et d’ornement en Région Wallonne
Delleur, Valery ULg; Marlier, Didier ULg

in Proceedings du 3ème colloque international de bactériologie francophone (2006, July)

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See detailViral infections in pigeons
Marlier, Didier ULg; Vindevogel, Henri ULg

in Veterinary Journal (2006), 172(1), 40-51

This review provides a current update on the major viral diseases of the domestic pigeon (Columba livia domestica), based on scientific reports and clinical experience. Paramyxovirus 1, adenovirus ... [more ▼]

This review provides a current update on the major viral diseases of the domestic pigeon (Columba livia domestica), based on scientific reports and clinical experience. Paramyxovirus 1, adenovirus, rotavirus, herpesvirus 1, poxvirus and circovirus infections are described according to common clinical signs and target tissues. Since pigeons are sometimes treated as if they were poultry, the review also summarises the common viral infections of poultry for which pigeons are considered resistant. It is hoped that the review will provide a useful reference for veterinarians and others and offer advice on the diagnosis, treatment and prevention of the major infectious diseases of pigeons. (c) 2005 Elsevier Ltd. All rights reserved. [less ▲]

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See detailTolerance study in broiler chickens after the oral administration of doxycycline with the drinking water
Cambier, Carole ULg; Marlier, Didier ULg; De Busser, J

in Journal of Veterinary Pharmacology and Therapeutics (2006)

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See detailComparaison de l’antibiorésistance vis-à-vis de la fluméquine et de l’enrofloxacine chez des souches d’Escherichia coli d’oiseaux de compagnie ou d’oiseaux de production
Marlier, Didier ULg

in Proceedings du 3ème colloque international de bactériologie francophone (2006)

Les résistances simples ou conjointes à la fluméquine et à l’enrofloxacine sont significativement plus rares chez les oiseaux de compagnie que chez ceux de production et semblent être dues à une sélection ... [more ▼]

Les résistances simples ou conjointes à la fluméquine et à l’enrofloxacine sont significativement plus rares chez les oiseaux de compagnie que chez ceux de production et semblent être dues à une sélection directe après traitement. Chez les oiseaux de production la fréquence des souches résistantes n’est influencée ni par l’âge, ni par la possibilité ou non de traiter les animaux (industrielle vs « bio-label »). Ces observations peuvent s’expliquer par le portage de souches résistantes dès la naissance des poussins; souches acquises au départ du cheptel reproducteur, ce dernier jouant un rôle disséminateur majeur lié à l’aspect typiquement pyramidal de la production avicole (faible nombre de parentaux qui produisent un grand nombre de volailles de production). [less ▲]

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See detailInfectious agents associated with epizootic rabbit enteropathy: Isolation and attempts to reproduce the syndrome
Marlier, Didier ULg; Dewrée, Roxane; Lassence, Cédric ULg et al

in Veterinary Journal (2006), 172(3), 493-500

Epizootic rabbit enteropathy (ERE), a highly lethal (30-80% mortality) disease of broiler rabbits aged 6-14 weeks, first appeared in 1997 in French intensive enclosed rabbitries and is of unknown ... [more ▼]

Epizootic rabbit enteropathy (ERE), a highly lethal (30-80% mortality) disease of broiler rabbits aged 6-14 weeks, first appeared in 1997 in French intensive enclosed rabbitries and is of unknown aetiology. Bacteriological, virological and parasitical examination of the intestinal contents of rabbits that had died either in spontaneous field cases or after experimental reproduction of ERE, were undertaken in an attempt to identify infectious agents that may play a role in the disease. Two bacterial strains, Clostridium perfringens and non-enteropathogenic Escherichia coli, were repeatedly isolated at high faecal counts from naturally infected animals. In field cases, a correlation between typical gross lesions of epizootic enteropathy and the presence of the alpha toxin of Cl. perfringens was observed (P < 0.0001; Chi-squared test). Although attempts to reproduce the disease by inoculation with different pools of cultivable bacterial strains failed, the disease was successfully reproduced by inoculation with one French and two Belgian samples of caecal contents. (c) 2005 Elsevier Ltd. All rights reserved. [less ▲]

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See detailEnteritis and enterotoxaemia in rabbits
Marlier, Didier ULg

in Duchesnes, C.; Menozzi, M. G.; Pelkonen, S. (Eds.) et al Diagnosis and typing of clostridia in medical and food microbiology (2006)

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See detailNouveaux animaux de compagnie : infections à Salmonella enterica et état sanitaire
Huybens, Nathalie ULg; Delleur, Valéry; Poulipoulis, Anastassios ULg et al

in Annales de Médecine Vétérinaire (2006), 150(4), 241-246

Human transmission of Salmonella enterica from new companion animals is usually suspected even though very few data according Salmonella infections in these animal species are available. The prevalence of ... [more ▼]

Human transmission of Salmonella enterica from new companion animals is usually suspected even though very few data according Salmonella infections in these animal species are available. The prevalence of Salmonella enterica infections in the digestive tract of 450 new companion animals ( 45 ferrets, 7 birds, 308 rodents, 82 rabbits and 8 reptiles) coming from pet shops or individual owners was determined by bacteriological examinations of anal swabs or pooled fresh faeces. Only one Salmonella Enteritidis strain was isolated from a ferret in a pet shop. The symptomatic or asymptomatic Salmonella carriage in ferrets, rodents and rabbits is thus not as frequent as what was suspected. [less ▲]

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