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See detailIN-VITRO EVALUATION OF A-5021 ANTI-VIRAL ACTIVITY AGAINST TESTUDINID HERPESVIRUS 3 AND INITIAL PHARMACOKINETIC STUDY IN HERMANN'S TORTOISE (Testudo hermanni)
Gandar, Frederic ULiege; Vrancken, Robert; Diez, Marianne ULiege et al

Conference (2013, April 23)

Testudinid herpesvirus infections in tortoises belonging to the Testudinidae family are well known for decades, but their pathogenesis remains poorly understood and treatments are often empirical. This ... [more ▼]

Testudinid herpesvirus infections in tortoises belonging to the Testudinidae family are well known for decades, but their pathogenesis remains poorly understood and treatments are often empirical. This study describes the in vitro evaluation of selected anti-herpesvirus compounds against Testudinid Herpesvirus 3 (THV-3). A-5021, a compound with known broad-spectrum anti-herpetic activity, showed to be 9 times more potent than acyclovir, with an EC50 of 13.2 µM and inducing a complete inhibition of viral replication at 37.7 µM. Initial pharmacokinetic parameters were determined after a single sub-cutaneous administration of 5 and 10 mg/kg in Hermann’s tortoises (Testudo hermanni, n=3). Blood samples were collected at different time points and plasma concentrations of A-5021 were determined. No adverse effects were clinically observed and plasma concentrations remained above the EC50 for 2.8 and 4.2 h after administration of 5 and 10 mg/kg, respectively. These preliminary data provide a basis for further proof-of-concept studies for a potential prophylactic or therapeutic treatment of THV-3 infection in tortoises [less ▲]

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See detailPyrosequencing of epizootic rabbit enteropathy inocula and rabbit caecal samples.
Huybens, N.; Houeix, J.; Licois, D. et al

in Veterinary Journal (2013), 196

The aetiological agent of epizootic rabbit enteropathy (ERE) is still unknown although a bacterial infection seems the most likely hypothesis. In this study, amplification of the V5 and V6 regions of ... [more ▼]

The aetiological agent of epizootic rabbit enteropathy (ERE) is still unknown although a bacterial infection seems the most likely hypothesis. In this study, amplification of the V5 and V6 regions of 16SrDNA from four virulent and two non-virulent caecal samples was performed using a pyrosequencing platform. The virulent samples did not group in the same cluster. The bacterial flora identified was both different and richer than the cultivable bacterial flora. These findings highlight the need for biomolecular techniques to identify the aetiological agent of ERE. [less ▲]

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See detailControl of Salmonella contamination of shell eggs – preharvest and postharvest methods: a review
Galis, Anca; Marcq, Christopher ULiege; Marlier, Didier ULiege et al

in Comprehensive Reviews in Food Science and Food Safety (2013), 12

Salmonella Enteritidis is one of the most prevalence foodborne pathogen, its most important reservoir being considered the shell egg. As the concerns related to the increasing human salmonellosis cases ... [more ▼]

Salmonella Enteritidis is one of the most prevalence foodborne pathogen, its most important reservoir being considered the shell egg. As the concerns related to the increasing human salmonellosis cases grow, the need for an application of preventive methods either at the farm level or during the processing steps is crucial for a better control of the foodborne outbreaks due to the consumption of this specific food product. This review focuses on the application of preventive methods at the farm level, on preharvest step, in order to reduce the risk of shell eggs contamination with Salmonella, especially S. Enteritidis, through a better control of the laying hens’ infection with this pathogen. As postharvest methods, a first approach is the egg storage conditions and the prevention of Salmonella spp. growth and multiplication. In addition, shell eggs may be subjected to eggshell decontamination, to reduce the risk of foodborne outbreaks. Several of these latter mentioned methods are already authorized to be put in place in different countries, as it is the case in the United States of America and Canada. Their efficacy has been proven and their use is regarded by some as mandatory for ensuring shell eggs safety for the consumers. [less ▲]

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See detailComparison of corticosterone, cortisol, triglycerides, aspartateaminotransferase and uric acid plasma concentrations during a foie gras production period in male mule ducks (Anas platyrhynchos X Cairina moschata)
Flament, Aline; Delleur, Valéry ULiege; Poulipoulis, Anastassios ULiege et al

in British Poultry Science (2012), 53(4), 408-414

1 Corticosterone, cortisol, triglycerides, aspartate aminotransferase (AST) and uric acid (UA) plasma concentration were measured at 8 and 12 weeks of age, at slaughtering and 45 min after an ... [more ▼]

1 Corticosterone, cortisol, triglycerides, aspartate aminotransferase (AST) and uric acid (UA) plasma concentration were measured at 8 and 12 weeks of age, at slaughtering and 45 min after an adrenocorticotrophic hormone (ACTH) stimulation test (8 weeks of age) in twelve male mule ducks. 2 No significant increase of corticosterone was found during the force feeding period. 3 Comparison of corticosterone and cortisol levels indicates that cortisol can be considered as a reliable acute stress indicator in future routine examinations. 4 Triglycerides and AST plasma concentrations progressively increased from pre-force feeding period to slaughtering. 5 UA plasma concentrations also increased from the start at 8 weeks of age to mid-force feeding time but no difference was noticed between mid-force feeding period and slaughtering. [less ▲]

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See detailGastrointestinal diseases
Marlier, Didier ULiege

Scientific conference (2012, February 11)

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See detailContraintes techniques et sanitaires de la production traditionnelle de pintade en Afrique subsaharienne
Boko, C; Kpodekon, T; Dahouda, M et al

in Annales de Médecine Vétérinaire (2012), 156

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See detailPrevalence of Salmonella serotypes in male mule ducks in Belgium
Flament, Aline; Soubbotina, Alexandra; Mainil, Jacques ULiege et al

in Veterinary Record : Journal of the British Veterinary Association (2012)

Salmonella species infections of male mule ducks were studied for 32 months in 100 flocks on nine duck farms in Belgium. The prevalence of Salmonella species infections changed significantly over time (P ... [more ▼]

Salmonella species infections of male mule ducks were studied for 32 months in 100 flocks on nine duck farms in Belgium. The prevalence of Salmonella species infections changed significantly over time (P<0.001) with infection rates of 50, 13.4, 6.7, 2.6 and 2.9 per cent, respectively, at the time of arrival on the farm, at three, six and nine weeks of age, and when the ducks left the breeding unit to enter the force-feeding rooms (at 11 or 12 weeks of age). During the study period, 95 strains of Salmonella were isolated, belonging to 11 serotypes. S Indiana (42.1 per cent) and S Regent (36.8 per cent) were the two most common serotypes, whereas S Typhimurium and S Enteritidis were found only once (1.1 per cent). All isolated strains were resistant to at least two antimicrobials, but resistance to more than five antimicrobials was observed in 21.6 per cent of the strains. [less ▲]

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See detailPneumonia with Aeromonas sobria in a Carpet Python
Gandar, Frederic ULiege; Szalo, Ioan Mihai ULiege; Marlier, Didier ULiege

Conference (2011, August 11)

Aeromonas sobria was isolated and identified upon post-mortem examination from the respiratory tract and the blood of a carpet python (Morelia spilota variegata). The snake was referred to the Faculty of ... [more ▼]

Aeromonas sobria was isolated and identified upon post-mortem examination from the respiratory tract and the blood of a carpet python (Morelia spilota variegata). The snake was referred to the Faculty of Veterinary Medicine of Liège for necropsy, just the day after it suddenly died without previous clinical sign. Lung and liver biopsies were performed and fixed in neutral buffered 10% formalin and paraffin embedded. Blood samples were collected via cardiocentesis, and air sac abscesses were cultured. Bacterial strains were identified as Aeromonas sobria by 16S rDNA sequencing. Based on histological and bacterial examinations, the death of this snake was attributed to a septicemia, following an acute primary, or secondary exudative pneumonia. Aeromonas sp. is established as a potential pathogen in reptiles. Among this genus, Aeromonas hydrophila is the most frequently isolated. A. sobria has been reported as a primary pathogen in farmed perch (Perca fluviatilis) and humans. Conversely, few data are available concerning the pathogenicity of A. sobria in reptiles. Other non-bacterial agents (virus, fungus, endoparasites) or predisposing factors (such as obesity) can also be responsible for respiratory tract disease in snakes . Unfortunately, in the current case, virological investigations were not performed. [less ▲]

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See detailEpizootic rabbit enteropathy: Comparison of PCR-based RAPD fingerprints from virulent and non-virulent samples
Huybens, Nathalie ULiege; Houeix, Julien ULiege; Licois, Dominique et al

in Veterinary Journal (2011), 190(3), 416-417

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See detailEpizootic rabbit enteropathy inoculum (TEC4) antibiograms and antibiotic fractionation
Huybens, Nathalie ULiege; Houeix, Julien ULiege; Licois, Dominique et al

in Veterinary Research Communications (2011), 35

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See detailVaccination strategies against myxomavius infections: are we really doing the best?
Marlier, Didier ULiege

in Tijdschrift voor Diergeneeskunde (2010), 134

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See detailLes techniques de biologie moléculaire d'analyse des populations bactériennes complexes
Huybens, Nathalie ULiege; Mainil, Jacques ULiege; Marlier, Didier ULiege

in Annales de Médecine Vétérinaire (2009), 153(2), 112-128

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See detailINOCULATION AND BACTERIAL ANALYSES OF FRACTIONS OBTAINED FROM THE REFERENCE INOCULUM TEC4 WHICH EXPERIMENTALLY REPRODUCES EPIZOOTIC RABBIT ENTEROPATHY
Huybens, Nathalie ULiege; Houeix, Julien ULiege; Licois, D. et al

in World Rabbit Science (2009), 17(4), 185-193

The aetiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial aetiology is the basis of current research. The fractionation of the ... [more ▼]

The aetiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial aetiology is the basis of current research. The fractionation of the reference inoculum (TEC4) is a major step towards finding the potential bacterial agent(s). In this study, TEC4 was fractionated by different techniques: centrifugation on discontinuous sucrose gradient, cell adherence and chloroform/ethanol treatment. The different fractions were inoculated into SPF rabbits and analyzed with classical bacteriological techniques. ERE was reproduced with two of the six fractions obtained. Four species never previously cultured from TEC were identified in the process but, to date, none of them seems to be the aetiology of ERE. [less ▲]

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See detailIS EPIZOOTIC RABBIT ENTEROPATHY (ERE) A BACTERIAL DISEASE?
Huybens, Nathalie ULiege; Houeix, Julien ULiege; Szalo, Ioan Mihai ULiege et al

in Proceeding of the 9th World Rabbit Congress (2008, June 12)

The etiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial etiology is at the basis of current research. The fractionation of the ... [more ▼]

The etiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial etiology is at the basis of current research. The fractionation of the reference inoculum (TEC4) is a major step to find the potential bacterial agent(s). In this study, TEC4 was fractionated with two techniques: centrifugation on discontinuous sucrose gradient then cell adhesion. Two selected fractions were inoculated to SPF rabbits and analyzed with classical bacteriological techniques. ERE was reproduced with both fractions. The 16S rDNA gene was amplified in all fractions and in three negative controls and subsequently analyzed with Restriction Fragment Length Polymorphism (RFLP) and Denaturating Gradient Gel Electrophoresis (DGGE). A difference in bacterial DNA composition was found between virulent and non-virulent fractions which reinforce the potential role of bacteria in the etiology of ERE. [less ▲]

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See detailNouveautés sur l’étiologie de l’entéropathie épizootique du lapin
Huybens, Nathalie; HOUEIX, Julien ULiege; Mainil, Jacques ULiege et al

Conference (2008, March 04)

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