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See detailAdhesion and growth inhibitory effect of chicken egg yolk antibody (IgY) on Salmonella enterica serovars Enteritidis and Typhimurium in vitro.
Chalghoumi, Raja; Thewis, André ULg; Beckers, Yves ULg et al

in Foodborne Pathogens and Disease (2009), 6(5), 593-604

The protective effects of powder preparation of egg yolk immunoglobulin Y (IgY), specific to Salmonella Enteritidis and Salmonella Typhimurium outer membrane proteins (OMP), against these two Salmonella ... [more ▼]

The protective effects of powder preparation of egg yolk immunoglobulin Y (IgY), specific to Salmonella Enteritidis and Salmonella Typhimurium outer membrane proteins (OMP), against these two Salmonella sp. serovars were investigated in vitro in two different assays: adhesion-prevention and growth-inhibition. The adhesion-prevention assay was conducted using polarized monolayers of the human intestinal epithelial Caco-2 cell line. First, the conditions of Salmonella adherence to Caco-2 cells were optimized, and interferences of bacteria with the transepithelial electrical resistance (TER) of fully differentiated Caco-2 cell monolayers and the lactate dehydrogenase release upon exposure of the cells to Salmonella were evaluated. Both Salmonella sp. serovars were able to adhere to Caco-2 cells and decreased TER. Results from the adhesion-prevention assay demonstrated that specific IgY reduced the decrease in TER of the infected Caco-2 cell monolayers and blocked the Salmonella sp. adhesion in a concentration-dependent manner (p < 0.05). Nonspecific IgY also exhibited an inhibitory effect on these two parameters, but to a lesser extent than that of the specific IgY (p < 0.05). The protective effect of nonspecific IgY could be attributed to the low-density lipoprotein component of the water-soluble fraction of egg yolks that may not have been eliminated during ultrafiltration. The growth-inhibition assay revealed that specific IgY had an inhibitory effect on the bacterial growth, markedly during the late exponential phase, whereas nonspecific IgY failed to do so. Taken together, these results suggest that the in vitro growth inhibitory effect of specific IgY on Salmonella spp. resulted from the specific binding activity of these IgY to Salmonella sp. OMP. Passive immunization with Salmonella sp. OMP-specific IgY could thus be useful to prevent Salmonella colonization in broiler chickens and the subsequent carcass contamination during processing. [less ▲]

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See detailEffects of feed supplementation with specific hen egg yolk antibody (immunoglobin Y) on Salmonella species cecal colonization and growth performances of challenged broiler chickens
Chalghoumi, Raja; Marcq, Christopher ULg; Thewis, André ULg et al

in Poultry Science (2009), 88(10), 2081-2092

Anti-Salmonella spp. egg yolk antibodies (IgY) simultaneously directed against Salmonella Enteritidis and Salmonella Typhimurium were tested to determine if their inclusion in feed decreased Salmonella ... [more ▼]

Anti-Salmonella spp. egg yolk antibodies (IgY) simultaneously directed against Salmonella Enteritidis and Salmonella Typhimurium were tested to determine if their inclusion in feed decreased Salmonella spp. cecal colonization in experimentally infected broiler chickens. Egg yolk powder (EYP) was obtained by freeze-drying egg yolks containing anti-Salmonella spp. Immunoglobin Y was included in feed at 5 levels of concentration (0 to 5%). Feeds were formulated to similar nutrient levels and provided for ad libitum intake from d 1 to 28. Three days after initiation of feed treatments (d 4), chickens were co-challenged with equal numbers of Salmonella Enteritidis and Salmonella Typhimurium (2x10(6) cfu/bird). Cecal samples were recovered weekly over the experimental period (d 7 to 28) to enumerate Salmonella spp. The effect of anti-Salmonella spp. IgY feed supplementation on growth performance of infected chickens was also evaluated during the same period. In comparison with the positive control treatment (PC), treatments involving EYP (T1, T2, T3, T4, and T5), whether containing anti-Salmonella spp. IgY or not, significantly improved (P<0.05) the growth performance of challenged chickens, but without reaching the performance levels of nonchallenged chickens (NC1 and NC2). However, no link can be established between the enhancement in growth performance of challenged birds and their contamination levels by Salmonella because in-feed incorporation of EYP had no significant effect on cecal colonization by Salmonella. Furthermore, the comparison of the 5 anti-Salmonella spp. IgY concentration levels in feed did not reveal any anti-Salmonella spp. IgY concentration effect on growth performance and Salmonella cecal colonization. These results suggest that anti-Salmonella spp. IgY would undergo denaturation and degradation after their passage through the animal gastrointestinal tract and reveal that components of EYP other than specific antibodies have a beneficial effect on growth performance. [less ▲]

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See detailDéveloppement d’une stratégie d’immunisation passive du poulet de chair vis-à-vis de Salmonella Enteritidis et Typhimurium à l’aide d’anticorps du jaune d’œuf
Marcq, Christopher ULg; Chalghoumi, RAJA ULg; Beckers, Yves ULg et al

in 8èmes JRA : Synthèses et textes (2009)

The present study investigates the potential of oral immunotherapy using hen egg yolk immunoglobulins (IgY) as a strategy to reduce the impact of Salmonella contamination on broilers. In a first step ... [more ▼]

The present study investigates the potential of oral immunotherapy using hen egg yolk immunoglobulins (IgY) as a strategy to reduce the impact of Salmonella contamination on broilers. In a first step, hyperimmune eggs were obtained from laying hens immunized using Salmonella Enteritidis (SE) and Typhimurium (ST) outer membrane proteins in emulsion with Freund adjuvants. This led to the production of high levels of IgY antibodies directed against the two Salmonella serovars in egg yolks. Furthermore, no adverse affects were detected on mortality or laying rate in hens injected with this vaccine. Powders were obtained from these eggs by spray- or freeze-drying the whole yolk. In order to study the effect of these powders on broiler’s growth performances, a challenge trial was conducted. 384 Salmonella spp.-free day-of-hatch chickens received supplemented feed (5 % freeze-dried egg yolk powder) since arrival, and were infected at day four with 106 cfu of SE and ST per animal. We found a significant improvement in growth performances for the groups receiving the yolk-enriched diets. Nevertheless, none of the supplement concentrations allowed to raise the same body weight after 28 days of complementation than in uninfected broilers (p < 0,05). Interestingly, nonimmune egg yolk powder also exhibited a positive effect on performances of broilers experimentally infected with SE and ST. Our results suggest that passive immunization through egg yolk powders could be useful to reduce negative effects of Salmonella infection on broilers growth performances. [less ▲]

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See detailContamination of poultry flocks by the human pathogen Campylobacter spp. and strategies to reduce its prevalence at the farm level.
Vandeplas, Sabrina ULg; Marcq, Christopher ULg; Dubois Dauphin, Robin et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2008), 12(3), 317-334

Enteric Campylobacter spp. bacteria are human pathogens that frequently contaminate poultry flocks. Consumption of products from poultry origin may then lead to acute bacterial enteritis called ... [more ▼]

Enteric Campylobacter spp. bacteria are human pathogens that frequently contaminate poultry flocks. Consumption of products from poultry origin may then lead to acute bacterial enteritis called campylobacteriosis of which prevalence is increasing for about ten years in Europe. This review summarizes Campylobacter epidemiological data, risk factors for contamination in poultry flocks and conceivable strategies to control this pathogen. [less ▲]

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See detailSurvival of yolk’s immunoglobulins directed against Salmonella Enteritidis and Salmonella Typhimurium in the gastro-intestinal tract of the broiler chicken
Marcq, Christopher ULg; Graide, Virginie; Beckers, Yves ULg et al

in Proceedings of the Thirteenth Conference on Food Microbiology (2008)

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See detailSelection of bacteria to decrease in vitro growth of Campylobacter jejuni and Campylobacter coli and characterization of their antagonistic activities
Marcq, Christopher ULg; Didderen, Isabelle; Dubois Dauphin, Robin ULg et al

in Proceedings of the Eleventh Conference on Food Microbiology (2006)

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