In vivo assessment of the virulence of five Salmonella enterica serotypes isolated from helmeted guinea fowl (Numida meleagris) in Benin
; ; et al
in International Research Journal of Microbiology [=IRJM] (2011), 2Detailed reference viewed: 11 (1 ULg)
La maladie de l'oedème du porcelet : Qui? Comment? Pourquoi?
Scientific conference (2010, November)Detailed reference viewed: 20 (0 ULg)
Evaluation of a hydro-alcoholic solution as pre-surgical hand antisepsis in a veterinary setting.
Verwilghen, Denis ; ; Mainil, Jacques et al
Conference (2010, July 02)
Introduction: Despite the fact that presurgical antiseptic hand treatment of surgical staff has since become a worldwide accepted procedure, surgical site infection is still one of the most frequent types ... [more ▼]
Introduction: Despite the fact that presurgical antiseptic hand treatment of surgical staff has since become a worldwide accepted procedure, surgical site infection is still one of the most frequent types of nosocomial infections. Many products have been used for hand antisepsis, but the popularity of alcoholic rubs amongst human surgeons is increasing as they have shown to provide a rapid and immediate action, are considerably faster than disinfecting soap scrubs and cause less skin damage after repeated use. The purpose of this study was 1) to identify surgical hand antisepsis habits amongst veterinary surgery specialists in Europe (ECVS) and the United States (ACVS), 2) to compare povidone iodine and chlorhexidine gluconate scrubs with a hydro-alcoholic rub hand antisepsis protocol and 3) to evaluate the usefulness of a hydro-alcoholic rub solution in a veterinary surgical setting. Materials and Methods: Emails were sent to 1300 Diplomates to invite them to participate to an online survey in order to obtain an idea about pre-surgical hand disinfection techniques. In a preliminary trial the efficiency of 4% chlorhexidine gluconate, 7.5 % PVP-iodine and an alcoholic solution containing 45% 2-propanol, 30% 1-propanol, 0.2% mecetronium ethylsulphate (Sterillium®) in bacterial reduction on hands was compared. In a clinical trial, the suitability of Sterillium® was assessed in an equine and small animal set up during surgery procedures. Fingertips were pressed on blood agar plates and Gassner plates prior to hand antisepsis (PHA), after handantisepsis (AHA) and three hours after wearing sterile gloves (AG) in the preliminary trial or at the end of surgery (AS) in the clinical trial. Bacterial counts (colony forming units : CFU’s) were obtained after 24 h of incubation of the plates. The obtained values of CFU from PHA, AHA, AG and AS were expressed as log10 values. For each sample, a reduction factor (RF) was obtained from the difference of log10 pre-value and log10 post-value. An ANOVA comparison between the effects of the different antisepsis protocols on the mean log10 CFU values and RF’s in function of the different steps was established. Results: A 42.6% response rate was obtained for the survey. Most surgeons’ still use a disinfecting soap only (79.9%) for hand antisepsis prior to surgery, the majority based on chlorhexidine gluconate (81.4%). Significant differences were found between immediate and sustained activities of the different products tested. Sterillium® was shown to have significantly lower LSM log10 CFU at AG compared to both other products. At AHA, povidone iodine revealed to have significantly higher LSM log10 CFU than Sterillium® and chlorhexidine gluconate, with the last two products having comparable activities. Reduction factors for the Sterillium® were significantly greater than for the other products. Only RF1 was comparable between Sterillium® and chlorhexidine gluconate. In the clinical trial, no significant differences were found between surgeons regarding LSM log10 CFU after hand antisepsis, neither between RF from samples taken at the small animal versus the equine surgery theatre. Discussion: This study confirms that Sterillium® is more effective in reducing bacterial counts on hands prior to surgery in a veterinary setting as are chlorhexidine gluconate (CHX) and povidone iodine soap. Moreover, they provide better tolerance and compliance to surgical hygiene protocols. Despite this, veterinary surgeons from all over the world still prefer the use of CHX soap, which has far more disadvantages than commonly accepted. Apart from the better skin tolerance and the absence of known resitance to the product, the use of Sterillium® offers the advantage of a fast (1.5 minute) surgical handantisepsis. This study shows that, as previously reported for human medicine, Sterillium® can safely be used in a veterinary surgical setting. [less ▲]Detailed reference viewed: 232 (6 ULg)
Final year thesis at the Faculty of Veterinary Medicine of the University of Liège : A new requirement, a new challenge!
Duchesnes, Christiane ; ; Mainil, Jacques
Conference (2010)Detailed reference viewed: 12 (0 ULg)
Antibiorésistance: une maladie génétique des bactéries.
Mainil, Jacques ; Mainil-Duchesnes, Christiane ; Muylaert, Adeline
Conference (2010)Detailed reference viewed: 17 (0 ULg)
Mécanismes de résistance des bactéries aux antibiotiques
Conference (2010)Detailed reference viewed: 17 (0 ULg)
Principales maladies bactériennes zoonotiques des animaux de compagnie (chiens et chats).
Conference (2010)Detailed reference viewed: 17 (3 ULg)
Enterotoxaemia-like syndrome and Clostridium perfringens in veal calves
Muylaert, Adeline ; ; Duprez, Jean-Noël et al
in Veterinary Record : Journal of the British Veterinary Association (2010), 167Detailed reference viewed: 42 (3 ULg)
Initial adherence of EPEC, EHEC and VTEC to host cells.
Bardiau, Marjorie ; Szalo, Ioan Mihai ; Mainil, Jacques
in Veterinary Research (2010), 41(5), 57
Initial adherence to host cells is the first step of the infection of enteropathogenic Escherichia coli (EPEC), enterohaemorrhagic Escherichia coli (EHEC) and verotoxigenic Escherichia coli (VTEC) strains ... [more ▼]
Initial adherence to host cells is the first step of the infection of enteropathogenic Escherichia coli (EPEC), enterohaemorrhagic Escherichia coli (EHEC) and verotoxigenic Escherichia coli (VTEC) strains. The importance of this step in the infection resides in the fact that (1) adherence is the first contact between bacteria and intestinal cells without which the other steps cannot occur and (2) adherence is the basis of host specificity for a lot of pathogens. This review describes the initial adhesins of the EPEC, EHEC and VTEC strains. During the last few years, several new adhesins and putative colonisation factors have been described, especially in EHEC strains. Only a few adhesins (BfpA, AF/R1, AF/R2, Ral, F18 adhesins) appear to be host and pathotype specific. The others are found in more than one species and/or pathotype (EPEC, EHEC, VTEC). Initial adherence of EPEC, EHEC and VTEC strains to host cells is probably mediated by multiple mechanisms. [less ▲]Detailed reference viewed: 20 (4 ULg)
Prevalence, molecular typing, and antibiotic sensitivity of enteropathogenic, enterohaemorrhagic, and verotoxigenic Escherichia coli isolated from veal calves.
Bardiau, Marjorie ; Muylaert, Adeline ; Duprez, Jean-Noël et al
in Tijdschrift voor Diergeneeskunde (2010), 135(14-15), 554-8
Cattle are considered to be an important reservoir of enterohaemorrhagic Escherichia coli (EHEC) and verotoxigenic Escherichia coli (VTEC) strains that can cause disease in humans, and numerous studies of ... [more ▼]
Cattle are considered to be an important reservoir of enterohaemorrhagic Escherichia coli (EHEC) and verotoxigenic Escherichia coli (VTEC) strains that can cause disease in humans, and numerous studies of the prevalence of these strains in cattle (focusing mainly on dairy and beef cattle) have been carried out in different regions of Europe, Asia, and America. To date, only a few studies of veal calves have been published focusing on EHEC strains belonging to the O157 serogroup EHEC, whereas EHEC and VTEC can belong to hundreds of different serotypes (many of which are as dangerous to humans as the O157:H7 EHEC, such as strains of the O26, O91, O103, O111, O113 and O145 serogroups). The aim of this study was to investigate the presence of enteropathogenic Escherichia coli (EPEC), EHEC, and VTEC strains in veal calves in Belgium and to characterize the positive isolates (serogroups, virulence-associated factor-encoding genes and antibiotic resistance profiles). The prevalence of EPEC, EHEC, and VTEC strains in faecal samples from veal calves in Belgium was found to be 11.7% (6.5% of the calves were found to be positive for EPEC strains, 2.6% for EHEC, and 3.9% for VTEC strains). No O157:H7 EHEC Strain was identified, but three calves were found to carry strains belonging to the O26 and O111 serogroups. The results of antibiotic sensitivity tests showed a high level of resistance (83% of strains were resistant or intermediate resistant to five or more antibiotics of the 13 tested antibiotics), which might be caused by the frequent use of antibiotics in veterinary practice. [less ▲]Detailed reference viewed: 26 (1 ULg)
Enteropathogenic (EPEC), enterohaemorragic (EHEC) and verotoxigenic (VTEC) Escherichia coli in wild cervids
Bardiau, Marjorie ; Grégoire, Fabien ; Muylaert, Adeline et al
in Journal of Applied Microbiology (2010), 109(6), 2214-2222Detailed reference viewed: 40 (6 ULg)
Typing of Staphylococcus aureus strains isolated from milk of cows with subclinical mastitis in Dakar, Senegal
; ; et al
in Bulletin of Animal Health and Production in Africa = Bulletin des Santé et Production Animales en Afrique (2010), 58Detailed reference viewed: 31 (3 ULg)
Cattle enterotoxaemia and Clostridium perfringens: description, diagnosis and prophylaxis
; Mainil, Jacques ; Linden, Annick
in Veterinary Record : Journal of the British Veterinary Association (2010), 167(1), 13-22Detailed reference viewed: 68 (8 ULg)
Dialogues moléculaires entre bactéries pathogènes et cellules hôtes : l'exemple des souches pathogènes d'Escherichia coli
Scientific conference (2009, December)Detailed reference viewed: 1 (0 ULg)
Zoonoses for the Medicval Students by a Veterinarian
Scientific conference (2009, November)Detailed reference viewed: 2 (0 ULg)
Biomolecular comparison of bacteriological caecal flora with and without Epizootic Rabbit Enteropathy (ERE) contamination
Huybens, Nathalie ; Houeix, Julien ; et al
Poster (2009, April 01)Detailed reference viewed: 48 (18 ULg)
Infections from bites and scratches from dogs, cats and rats
Scientific conference (2009, January)
Leptospirosis of domestic animals and hazards for humans
Scientific conference (2009, January)Detailed reference viewed: 3 (3 ULg)