References of "Mainil, Jacques"
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See detailLes techniques de biologie moléculaire d'analyse des populations bactériennes complexes
Huybens, Nathalie ULg; Mainil, Jacques ULg; Marlier, Didier ULg

in Annales de Médecine Vétérinaire (2009), 153(2), 112-128

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See detailEnterohaemorrhagic Escherichia coli serogroup O111 inhibits NF-(kappa)B-dependent innate responses in a manner independent of a type III secreted OspG orthologue.
Nobe, Rika; Nougayrede, Jean*-Philippe; Taieb, Frederic et al

in Microbiology (2009), 155(Pt 10), 3214-25

Enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) inject a repertoire of effector proteins into host cells via a type III secretion system (T3SS) encoded by the locus of enterocyte ... [more ▼]

Enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) inject a repertoire of effector proteins into host cells via a type III secretion system (T3SS) encoded by the locus of enterocyte effacement (LEE). OspG is an effector protein initially identified in Shigella that was shown to inhibit the host innate immune response. In this study, we found ospG homologues in EHEC (mainly of serogroup O111) and in Yersinia enterocolitica. The T3SS encoded by the LEE was able to inject these different OspG homologues into host cells. Infection of HeLa cells with EHEC O111 inhibited the NF-kappaB-dependent innate immune response via a T3SS-dependent mechanism. However, an EHEC O111 ospG mutant was still able to inhibit NF-kappaB p65 transfer to the nucleus in infected cells stimulated by tumour necrosis factor alpha (TNF-alpha). In addition, no difference in the inflammatory response was observed between wild-type EHEC O111 and the isogenic ospG mutant in the rabbit ligated intestinal loop model. These results suggest that OspG is not the sole effector protein involved in the inactivation of the host innate immune system during EHEC O111 infection. [less ▲]

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See detailPreliminary characterization of jejunocyte and colonocyte cell lines isolated by enzymatic digestion from adult and young cattle.
Loret, Suzanne; Rusu, Dorina; Moualij, Benaissa El et al

in Research in Veterinary Science (2009), 87(1), 123-32

In the present study we developed an enzymatic approach (through the use of collagenase and dispase) to isolate bovine intestinal epithelial cells. Using this method, freshly isolated jejunocytes could be ... [more ▼]

In the present study we developed an enzymatic approach (through the use of collagenase and dispase) to isolate bovine intestinal epithelial cells. Using this method, freshly isolated jejunocytes could be distinguished from simultaneously isolated colonocytes, as the jejunocytes specifically exhibited the small intestinal peptidase gene transcript, as well as an active alkaline phosphatase. The transformation of both types of cell suspension was performed by retroviral infection, using reproduction-defective viruses bearing the gene coding for the large T antigen of the leukaemia simian virus (SV40). The success of the transfection was demonstrated by (1) a significant increase in cell passage numbers (52-53 vs. 7 passages for non-transfected cells), (2) the detection of both the large T transcript and the large T antigen in transformed cells. Possible contamination and progressive substitution of bovine primocultures by non-bovine lineages available in the laboratory was excluded, as the transformed cells presented a bovine typical karyotype. Most transfected cells kept an epithelial morphology after transformation. They also maintained the expression of FABP and enterocyte specific enzymes (brush-border associated maltase and IAP). However, levels of specific activity of these enzymes were low, suggesting that cell differentiation is not completely achieved under the applied culture conditions. [less ▲]

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See detailINOCULATION AND BACTERIAL ANALYSES OF FRACTIONS OBTAINED FROM THE REFERENCE INOCULUM TEC4 WHICH EXPERIMENTALLY REPRODUCES EPIZOOTIC RABBIT ENTEROPATHY
Huybens, Nathalie ULg; Houeix, Julien ULg; Licois, D. et al

in World Rabbit Science (2009), 17(4), 185-193

The aetiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial aetiology is the basis of current research. The fractionation of the ... [more ▼]

The aetiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial aetiology is the basis of current research. The fractionation of the reference inoculum (TEC4) is a major step towards finding the potential bacterial agent(s). In this study, TEC4 was fractionated by different techniques: centrifugation on discontinuous sucrose gradient, cell adherence and chloroform/ethanol treatment. The different fractions were inoculated into SPF rabbits and analyzed with classical bacteriological techniques. ERE was reproduced with two of the six fractions obtained. Four species never previously cultured from TEC were identified in the process but, to date, none of them seems to be the aetiology of ERE. [less ▲]

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See detailIS EPIZOOTIC RABBIT ENTEROPATHY (ERE) A BACTERIAL DISEASE?
Huybens, Nathalie ULg; Houeix, Julien ULg; Szalo, Ioan Mihai ULg et al

in Proceeding of the 9th World Rabbit Congress (2008, June 12)

The etiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial etiology is at the basis of current research. The fractionation of the ... [more ▼]

The etiology of epizootic rabbit enteropathy (ERE) is still unknown despite ten years of continuous research. A putative bacterial etiology is at the basis of current research. The fractionation of the reference inoculum (TEC4) is a major step to find the potential bacterial agent(s). In this study, TEC4 was fractionated with two techniques: centrifugation on discontinuous sucrose gradient then cell adhesion. Two selected fractions were inoculated to SPF rabbits and analyzed with classical bacteriological techniques. ERE was reproduced with both fractions. The 16S rDNA gene was amplified in all fractions and in three negative controls and subsequently analyzed with Restriction Fragment Length Polymorphism (RFLP) and Denaturating Gradient Gel Electrophoresis (DGGE). A difference in bacterial DNA composition was found between virulent and non-virulent fractions which reinforce the potential role of bacteria in the etiology of ERE. [less ▲]

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See detailComparison of bovine and human O26 EHEC strains by the Whole Genome PCR Scanning
Bardiau, Marjorie ULg; Ogura, Y.; Hayashi, T. et al

Conference (2008, June)

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See detailNouveautés sur l’étiologie de l’entéropathie épizootique du lapin
Huybens, Nathalie; HOUEIX, Julien ULg; Mainil, Jacques ULg et al

Conference (2008, March 04)

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See detailComparison of bovine and human O26 EHEC strains by the Whole Genome PCR Scanning
Bardiau, Marjorie ULg; Ogura, Y.; Hayashi, T. et al

Conference (2008, March)

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See detailCreating hybrid proteins by insertion of exogenous peptides into permissive sites of a class A beta-lactamase
Ruth, Nadia ULg; Quinting, Brigitte; Mainil, Jacques ULg et al

in FEBS Journal (2008), 275

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See detailPurification of the recombinant beta2 toxin (CPB2) from an enterotoxaemic bovine Clostridium perfringens strain and production of a specific immune serum
Lebrun, Maud; Filée, Patrice ULg; Galleni, Moreno ULg et al

in Protein Expression & Purification (2007), 55(1), 119-131

Overgrowth of Clostridium perfringens clones with production of one or more of its toxin(s) results in diverse digestive and systemic pathologies in human and animals, such as cattle enterotoxaemia. The ... [more ▼]

Overgrowth of Clostridium perfringens clones with production of one or more of its toxin(s) results in diverse digestive and systemic pathologies in human and animals, such as cattle enterotoxaemia. The so-called beta2 toxin (CPB2) is the most recently described major toxin produced by C perfringens. In this study, the cpb2 ORF (cpb2FM) from a cattle C perfringens-associated enterotoxaemia was cloned and sequenced. The cpb2FM and its deduced nucleotide sequence clearly corresponded to the epb2 allele considered as "consensus" and not to "atypical" allele, despite its "non-porcine" origin. Expression assays of the recombinant toxin CPB2FM were performed in Escherichia coli and Bacillus subtilis with the expression vector pBLTS72, and by genomic integration by double recombination in B. subtilis. Highest level of production was obtained with the expression vector in B. subtilis 168 strain. The recombinant CPB2FM protein was purified and a specific rabbit polyclonal antiserum was produced. Polyclonal antibodies could detect CPB2 production in supernatants of C. perfringens from enterotoxaemic cattle. (C) 2007 Elsevier Inc. All rights reserved. [less ▲]

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