References of "Mainil, Jacques"
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See detailContraintes techniques et sanitaires de la production traditionnelle de pintade en Afrique subsaharienne
Boko, C; Kpodekon, T; Dahouda, M et al

in Annales de Médecine Vétérinaire (2012), 156

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See detailO157:H7 and O104:H4 Vero/Shiga toxin-producing Escherichia coli outbreaks: respective role of cattle and humans
Piérard, D; De Greve, H; Haesebrouck, F et al

in Veterinary Research (2012), 43

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See detailDiagnostic strategy for identifying avian pathogenic Escherichia coli based on four patterns of virulence genes
Schouler, C; Schaeffer, B; Brée, A et al

in Journal of Clinical Microbiology (2012), 50(5), 1673-1678

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See detailPrevalence of Salmonella serotypes in male mule ducks in Belgium
Flament, Aline; Soubbotina, Alexandra; Mainil, Jacques ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2012)

Salmonella species infections of male mule ducks were studied for 32 months in 100 flocks on nine duck farms in Belgium. The prevalence of Salmonella species infections changed significantly over time (P ... [more ▼]

Salmonella species infections of male mule ducks were studied for 32 months in 100 flocks on nine duck farms in Belgium. The prevalence of Salmonella species infections changed significantly over time (P<0.001) with infection rates of 50, 13.4, 6.7, 2.6 and 2.9 per cent, respectively, at the time of arrival on the farm, at three, six and nine weeks of age, and when the ducks left the breeding unit to enter the force-feeding rooms (at 11 or 12 weeks of age). During the study period, 95 strains of Salmonella were isolated, belonging to 11 serotypes. S Indiana (42.1 per cent) and S Regent (36.8 per cent) were the two most common serotypes, whereas S Typhimurium and S Enteritidis were found only once (1.1 per cent). All isolated strains were resistant to at least two antimicrobials, but resistance to more than five antimicrobials was observed in 21.6 per cent of the strains. [less ▲]

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See detailComparison of bovine and human O26 EHEC strains by the Whole Genome PCR Scanning
Bardiau, Marjorie ULg; Ogura, Y.; Mainil, Jacques ULg et al

Conference (2011, December)

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See detailEpidemiology of Q fever in animals and humans in the 21st century
Mainil, Jacques ULg; Porter, S.; Czaplicky, G. et al

Conference (2011)

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See detailE.coli virulence factors
Mainil, Jacques ULg

Conference (2011)

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See detailTyping of O26 enterohaemorrhagic and enteropathogenic Escherichia coli isolated from humans and cattle with IS621 multiplex PCR-based fingerprinting.
Mainil, Jacques ULg; Bardiau, Marjorie ULg; Ooka, T. et al

in Journal of Applied Microbiology (2011), 111(3), 773-86

AIMS: This study evaluated a typing method of O26:H11 enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) based on the variation in genomic location and copy numbers of IS621. METHODS ... [more ▼]

AIMS: This study evaluated a typing method of O26:H11 enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) based on the variation in genomic location and copy numbers of IS621. METHODS AND RESULTS: Two multiplex PCRs, targeting either the left (5') or right (3') IS/chromosome junction of 12 IS621 insertion sites and one PCR specific of another truncated copy, were developed. Thirty-eight amplification profiles were observed amongst a collection of 69 human and bovine O26:H11 EHEC and EPEC. Seventy-one per cent of the 45 EHEC and EPEC with identical IS621 fingerprints within groups of two, three or four isolates had >85% pulsed field gel electrophoresis (PFGE) profile similarity, including four groups of epidemiologically related EHEC or EPEC, while most of the groups had <85% similarity between each others. Epidemiologically related EHEC from each of three independent outbreaks in Japan and Belgium also exhibited identical IS621 fingerprints and PFGE profiles. CONCLUSIONS: The IS621 fingerprinting and the PFGE are complementary typing assays of EHEC and EPEC; though, the former is less discriminatory. SIGNIFICANCE AND IMPACT OF THE STUDY: The IS621 printing method represents a rapid (24 h) first-line surveillance and typing assay, to compare and trace back O26:H11 EHEC and EPEC during surveys in farms, multiple human cases and outbreaks. [less ▲]

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See detailQ Fever: Current State of Knowledge and Perspectives of Research of a Neglected Zoonosis
Porter, Sarah ULg; Czaplicki, G.; Mainil, Jacques ULg et al

in International Journal of Microbiology (2011)

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See detailQ fever IN JApaN: an update REVIEW
Porter, Sarah ULg; Czaplicki, G.; Mainil, Jacques ULg et al

in Veterinary Microbiology (2011), 149

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See detailEpizootic rabbit enteropathy: Comparison of PCR-based RAPD fingerprints from virulent and non-virulent samples
Huybens, Nathalie ULg; Houeix, Julien ULg; Licois, Dominique et al

in Veterinary Journal (2011), 190(3), 416-417

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See detailStudy of polymorphisms in tir, eae and tccP2 genes in enterohaemorrhagic and enteropathogenic Escherichia coli of serogroup O26.
Bardiau, Marjorie ULg; Labrozzo, S.; Mainil, Jacques ULg

in BMC Microbiology (2011), 11

BACKGROUND: Enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli are responsible for food poisoning (enteritis and enterotoxaemia) in humans in developed countries. Cattle are considered ... [more ▼]

BACKGROUND: Enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli are responsible for food poisoning (enteritis and enterotoxaemia) in humans in developed countries. Cattle are considered to be an important reservoir of EHEC and EPEC strains for humans. Moreover, some of the strains, belonging to the O26, O111, O118 serogroups, for example, are also responsible for digestive disorders in calves. The Translocated intimin receptor (Tir), the intimin (Eae) and the Tir-cytoskeleton coupling protein (TccP) represent three virulence factors implicated in the intimate attachment of the bacteria to the eukaryotic cell. Major variants have already been described for these genes among the different serogroups but minor variations have not often been studied. In this study, we examined the polymorphisms of the tir, eae and tccP2 genes of O26 strains (EPEC and EHEC isolated from bovines and from humans) with the aim to determine whether these polymorphisms are host specific or not. RESULTS: Of the 70 tested strains, 10 strains (14% of the strains) presented one or several polymorphisms in the tir and eae genes, which have never previously been described. Concerning tccP2 detection, 47 of the 70 strains (67% of the strains) were found to be positive for this gene. Most of the strains were found to possess tccP2 variants described in strains of serogroup O26. Nevertheless, three strains had tccP2 genes respectively described in strains of serogroup O111, O103 and O55. Moreover, none of the polymorphisms was statistically specific to the bovine or the human isolates. Nevertheless, the two major variants of tccP2 were statistically associated with the pathotype (EPEC or EHEC). CONCLUSIONS: In conclusion, tir and eae gene polymorphisms were found not to be numerous and not to be predominantly synonymous. Moreover, no difference was observed between human and bovine strains regarding the presence of polymorphisms. Finally, some tccP2 variants appeared to be pathotype specific. Further investigations need to be performed on a larger number of strains in order to confirm this specificity. [less ▲]

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See detailSurgical hand antisepsis in veterinary practice: evaluation of soap scrubs and alcohol based rub techniques
Verwilghen, Denis ULg; Mainil, Jacques ULg; Mastrocicco, Emilie et al

in Veterinary Journal (2011), 190(3), 372-377

Recent evidence shows hydro-alcoholic solutions are more efficient than traditional medicinated soaps in the presurgical hand antisepsis of human surgeons. However, little veterinary literature is ... [more ▼]

Recent evidence shows hydro-alcoholic solutions are more efficient than traditional medicinated soaps in the presurgical hand antisepsis of human surgeons. However, little veterinary literature is available on the subject. The aims of this study were to compare the efficiency of medicinated soaps and a hydro-alcoholic solution prior to surgery using an in use testing method in a veterinary setting. A preliminary trial was performed that compared the mean Log10 number of bacterial colony forming units (CFU) and the reduction factors (RF) between 2 five-minute hand-scrubbing sessions using different soaps (povidone iodine (PVP) and chlorhexidine gluconate (CHX)) and the 1.5-min application of a hydro-alcoholic rub (Sterillium). A clinical in use trial comparing mean log10 number of bacterial CFU’s and RF for Sterillium and CHX soap was performed in a surgical set-up. Sampling was performed using finger printing on agar plates. Sterillium and CHX had a similar immediate effect, although the sustained effect was significantly better for Sterillium. PVP showed a significantly lower immediate and sustained effect. Sterillium showed good efficiency in the clinical trial. This study shows that, as previously reported for human medicine, Sterillium can safely be considered as an attractive alternative method for surgical hand antisepsis in a veterinary surgical setting. [less ▲]

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See detailEpizootic rabbit enteropathy inoculum (TEC4) antibiograms and antibiotic fractionation
Huybens, Nathalie ULg; Houeix, Julien ULg; Licois, Dominique et al

in Veterinary Research Communications (2011), 35

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See detailFarmer perceptions and pathological constraints in helmeted guinea fowl farming in the Borgou department in North-East Benin
Boko, C. K.; Kpodekon, M. T.; Farougou, S. et al

in African Journal of Agricultural Research (2011), 6

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