References of "Mainil, Jacques"
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See detailRésistance aux antibiotiques de souches d'Escherichia coli bovines et aviaires isolées en Algérie
Mellata, M.; Jacquemin, E.; Bakour, R. et al

in Annales de Médecine Vétérinaire (1998), 142

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See detailCaractérisation phénotypique et génotypique de la virulence potentielle de souches d'Escherichia coli bovines isolées en Algérie
Mellata, M.; Bakour, R.; Jacquemin, E. et al

in Annales de Médecine Vétérinaire (1998), 142

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See detailVirulence Plasmids of Enterotoxigenic Escherichia Coli Isolates from Piglets
Mainil, Jacques ULg; Daube, Georges ULg; Jacquemin, Etienne et al

in Veterinary Microbiology (1998), 62(4), 291-301

Virulence plasmids of 68 ETEC isolates from piglets belonging to different pathotypes and six ETEC isolates from calves with pathotypes typical of porcine ETEC were identified with seven virulence probes ... [more ▼]

Virulence plasmids of 68 ETEC isolates from piglets belonging to different pathotypes and six ETEC isolates from calves with pathotypes typical of porcine ETEC were identified with seven virulence probes for the heat-stable (STa and STb) and heat-labile (LT) enterotoxins, for the F4, F5, F6, and F41 fimbrial adhesion subunit, and also with five Rep probes for the RepFIA and RepFIB basic replicons, and the RepFIC family of basic replicons. With the exception of the F41 probe, the other virulence probes hybridized with at least one plasmid band of a size range from 65 to more than 100 Mda. Common associations of virulence factor-encoding genes on plasmid bands were: STb/LT, STa/F5, STa/F6, STa/STb. Other associations, STa/F4, STa/F4/F6, and STa/STb/LT/F6, were rarer. On the other hand the F4 adhesin-encoding genes were isolated on one plasmid band in all but three F4+ isolates. All but one of the 92 virulence plasmids which were studied have Rep probe hybridization profiles and replicon types typical of the uni- or multireplicon plasmids belonging to the various incompatibility groups of the F incompatibility complex. [less ▲]

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See detailThe prevalence of Clostridium perfringens in the horse
Griffiths, N. J.; Walton, J. R.; Edwards, G. B. et al

in Rev Med Microbiol (1998), 9

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See detailBovine Attaching and Effacing Escherichia Coli Possess a Pathogenesis Island Related to the LEE of the Human Enteropathogenic Escherichia Coli Strain E2348/69
Goffaux, F.; Mainil, Jacques ULg; Pirson, V. et al

in FEMS Microbiology Letters (1997), 154(2), 415-421

Attaching and effacing Escherichia coli (AEEC) has been described as a cause of diarrhea in calves. The molecular pathogenesis of AEEC was mainly studied in human enteropathogenic E. coli strain E2348/69 ... [more ▼]

Attaching and effacing Escherichia coli (AEEC) has been described as a cause of diarrhea in calves. The molecular pathogenesis of AEEC was mainly studied in human enteropathogenic E. coli strain E2348/69 in which the virulence correlated with the presence of a 35.4 kb pathogenesis island called LEE. We showed that several strains isolated from calves with diarrhea were able to produce attaching and effacing lesions in a rabbit ileal loop model and that they possess a pathogenesis island related to the LEE. Moreover, we showed that the LEE from bovine strains was inserted mainly at a different position in the chromosome compared to the human enteropathogenic E. coli strain E2348/69. [less ▲]

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See detailDifférences entre les pathotypes et les sérogroupes des Escherichia coli vérotoxinogènes isolées de veaux sains et celles isolées de veaux souffrant de diarrhée
Pohl, P.; Cleenwerk, I.; Imberechts, H. et al

in Annales de Médecine Vétérinaire (1997), 141

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See detailAntigens
Van Regenmortel, M; Tellam, R; Manteca, C et al

in Pastoret, PP; Blancou, J; Vannier, P (Eds.) et al Veterinary Vaccinology (1997)

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See detailPresence of pap-, sfa- and afa-related sequences in necrotoxigenic Escherichia coli isolates from cattle : evidence for new variants of the AFA family
Mainil, Jacques ULg; Jacquemin, E.; Hérault, F. et al

in Canadian Journal of Veterinary Research = Revue Canadienne de Recherche Vétérinaire (1997), 61

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See detailTyping of Bovine Attaching and Effacing Escherichia Coli by Multiplex in Vitro Amplification of Virulence-Associated Genes
China, B.; Pirson, V.; Mainil, Jacques ULg

in Applied and Environmental Microbiology (1996), 62(9), 3462-3465

Attaching and effacing Escherichia coli is a new causal agent of diarrhea in calves. Its major virulence factors are the intimin protein, encoded by the eaeA gene, and the Shiga-like toxins, encoded by ... [more ▼]

Attaching and effacing Escherichia coli is a new causal agent of diarrhea in calves. Its major virulence factors are the intimin protein, encoded by the eaeA gene, and the Shiga-like toxins, encoded by slt genes. Because the sequences of these genes are available, we selected specific primers to amplify each virulence gene so as to develop a new identification test based on multiplex amplification of virulence-associated genes. Of 30 tested strains, 14 were eaeA+, 15 were eaeA+ slt-I+, 1 was eaeA+ slt-I+ slt-II+, and 1 was eaeA+ slt-II+. The method proved in our hands to be fast and specific and in perfect correlation with the hybridization method. [less ▲]

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See detailL'entérotoxémie bovine: rôle de Clostridium perfringens
Daube, Georges ULg; Ginter, A; Manteca, C et al

Book published by Ministère de l'Agriculture et des Classes Moyennes (1996)

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See detailHybridization of 2,659 Clostridium Perfringens Isolates with Gene Probes for Seven Toxins (Alpha, Beta, Epsilon, Iota, Theta, Mu, and Enterotoxin) and for Sialidase
Daube, Georges ULg; Simon, Patricia ULg; Limbourg, Bernard et al

in American Journal of Veterinary Research (1996), 57(4), 496-501

OBJECTIVE--To genetically characterize Clostridium perfringens isolates for association of pathologic type with various diseases. DESIGN--Prospective study. SAMPLE POPULATION--2,659 C perfringens isolates ... [more ▼]

OBJECTIVE--To genetically characterize Clostridium perfringens isolates for association of pathologic type with various diseases. DESIGN--Prospective study. SAMPLE POPULATION--2,659 C perfringens isolates from various nonhuman animals species, human beings, and foods. PROCEDURE--Colony hybridization with DNA probes for 7 toxin (alpha, beta, epsilon, iota (subunits a and b), theta, mu, and enterotoxin) genes and 1 sialidase gene were performed to group the isolates by pathologic type. RESULTS--Enterotoxin-negative type-A isolates were the most common (2,575/2,659), were isolated from all sources, and were separated into 5 pathologic types. In cattle and horses with enterotoxemia, essentially only these pathologic types were identified. The enterotoxin-negative isolates of types C or D each had a single pathologic type. Type-C isolates were isolated only from swine with necrotic enteritis and type-D isolates from small ruminants with enterotoxemia, except that 1 type-D isolate was also found from a healthy fish. Type-B or -E isolates were not found. Among the 47 enterotoxin-positive isolates, 5 isolates from sheep or deer were type D and the other 42 were type A. These 42 isolates were grouped into 3 pathologic types: 1 type was isolated from samples of almost all origins, but the other 2 types were found in only 5 fish, 4 human beings, and 1 dog. CONCLUSIONS AND CLINICAL RELEVANCE--Genetic characterization of these isolates allowed identification of 11 different pathologic types. This approach may be useful in molecular diagnosis and prophylaxis of clostridial disease. [less ▲]

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See detailLa vaccination anticolibacillaire en pathologie digestive
Mainil, Jacques ULg

Conference (1995)

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See detailInfection respiratoire équine à Bordetella bronchiseptica
Vandevenne, S.; Caudron, I.; Serteyn, Didier ULg et al

in Annales de Médecine Vétérinaire (1995), 139

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See detailTyping of Clostridium Perfringens by in Vitro Amplification of Toxin Genes
Daube, Georges ULg; China, Bernard; Mainil, Jacques ULg et al

in Journal of Applied Bacteriology (1994), 77(6), 650-655

The strains of Clostridium perfringens are classified according to major toxins produced. Classically, this determination involves the seroneutralization of their lethal effect in mice. However, this ... [more ▼]

The strains of Clostridium perfringens are classified according to major toxins produced. Classically, this determination involves the seroneutralization of their lethal effect in mice. However, this method requires specific antisera and a large number of mice. In this work, a new typing method was developed based on the amplification of toxin genes by polymerase chain reaction (PCR). By combination of several pairs of primers, the toxinotype of a Cl. perfringens strain was determined by looking at the pattern of bands on an agarose gel electrophoresis. This mixture contained primers amplifying simultaneously a part of alpha-toxin, beta-toxin, epsilon-toxin and enterotoxin genes. In order to distinguish between toxinotype A and E, the l-toxin gene fragment must be amplified in a separate PCR reaction. Moreover, with the primers combination, in most cases, a PCR product corresponding to the alpha-toxin gene was obtained from direct enrichments of animal intestinal contents. [less ▲]

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See detailSpecific DNA probes to detect Escherichia coli strains producing cytotoxic necrotising factor type 1 or type 2
Oswald, E.; Pohl, P.; Jacquemin, E. et al

in Journal of Medical Microbiology (1994), 40

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See detailLa maladie de Lyme : une nouvelle maladie?
Mainil, Jacques ULg

in Annales de Médecine Vétérinaire (1994), 138

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See detailLes souches attachantes et effaçantes d'Escherichia coli d'origine bovine
Mainil, Jacques ULg; Pohl, P.

in Annales de Médecine Vétérinaire (1994), 138

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