References of "Mainil, Jacques"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailSerotypes and intimin types of intestinal and faecal strains of eae+ Escherichia coli from weaned pigs
Malik, A.; Toth, I.; Beutin, L. et al

in Veterinary Microbiology (2006), 114

Detailed reference viewed: 17 (4 ULg)
Full Text
Peer Reviewed
See detailImmunochemical, biomolecular and biochemical characterization of bovine epithelial intestinal primocultures
Rusu, D.; Loret, S.; Peulen, Olivier ULg et al

in BMC Cell Biology (2005), 6

Background: Cultures of enterocytes and colonocytes represent valuable tools to study growth and differentiation of epithelial cells. In vitro models may be used to evaluate passage or toxicity of drugs ... [more ▼]

Background: Cultures of enterocytes and colonocytes represent valuable tools to study growth and differentiation of epithelial cells. In vitro models may be used to evaluate passage or toxicity of drugs, interactions of enteropathogenes bacteria strains with intestinal epithelium and other physiologic or pathologic phenomenon involving the digestive tract. Results: Cultures of bovine colonocytes and jejunocytes were obtained from organoid-enriched preparations, using a combination of enzymatic and mechanical disruption of the intestine epithelium, followed by an isopicnic centrifugation discarding most single cells. Confluent cell monolayers arising from plated organoids exhibited epithelium typical features, such as the pavement-like structure, the presence of apical microvilli and tight junctions. Accordingly, cells expressed several markers of enterocyte brush border (i.e. maltase, alkaline phosphatase and fatty acid binding protein) as well as an epithelial cytoskeleton component (cytokeratin 18). However, enterocyte primocultures were also positive for the vimentin immunostaining (mesenchyme marker). Vimentin expression studies showed that this gene is constitutively expressed in bovine enterocytes. Comparison of the vimentin expression profile with the pattern of brush border enzymes activities, suggested that the decrease of cell differentiation level observed during the enterocyte isolation procedure and early passages of the primoculture could result from a post-transcriptional de-repression of vimentin synthesis. The low differentiation level of bovine enterocytes in vitro could partly be counteracted adding butyrate (1-2 mM) or using a glucose-deprived culture medium. Conclusion: The present study describes several complementary approaches to characterize bovine primary cultures of intestinal cells. Cultured cells kept their morphologic and functional characteristics during several generations. [less ▲]

Detailed reference viewed: 17 (1 ULg)
Full Text
Peer Reviewed
See detailDNA vaccination for the priming of neutralizing antibodies against non-immunogenic STa enterotoxin from enterotoxigenic Escherichia coli
Ruth, Nadia ULg; Mainil, Jacques ULg; Roupie, Virginie et al

in Vaccine (2005), 23(27), 3618-3627

In order to test the use of DNA vaccination for its capacity to induce antibodies against the non-immunogenic heat-stable enterotoxin STa from Escherichia coli, BALB/c mice were immunized with plasmid DNA ... [more ▼]

In order to test the use of DNA vaccination for its capacity to induce antibodies against the non-immunogenic heat-stable enterotoxin STa from Escherichia coli, BALB/c mice were immunized with plasmid DNA encoding hybrid proteins made by the insertion of wild type STa or insertion of the Cys6Ala, Cys17Ala and Cys6Ala-Cys17Ala STa mutants at positions 195 or 216 of the TEM-1 beta-lactamase. No STa specific antibodies could be detected after three plasmid injections, but a subsequent boost with native STa peptide was capable of inducing low levels of neutralizing antibodies, as tested in the suckling mouse assay. Highest STa specific responses were found in mice primed with the double mutated STa inserted in position 195. This plasmid induced highest T-cell responses to the TEM-1 protein, indicating that priming of helper T-cell responses to the carrier protein was essential. Mixed IgG1/IgG2a isotypes also reflected this T helper 1 type priming. Moreover, insertion into loop A of the TEM-1 carrier may be more suitable than insertion into loop B, because of reduced competition between carrier and hapten B cell responses. [less ▲]

Detailed reference viewed: 64 (10 ULg)
Full Text
Peer Reviewed
See detailMycoplasma bovis shares insertion sequences with Mycoplasma agalactiae and Mycoplasma mycoides subsp mycoides SC: Evolutionary and developmental aspects
Thomas, Anne; Linden, Annick ULg; Mainil, Jacques ULg et al

in FEMS Microbiology Letters (2005), 245(2), 249-255

Three new insertion elements, IS Mbov1, ISMbov2 and ISMbov3, which are closely related to ISMag1 (Mycoplasma agalactiae), ISMmy1 and IS1634 (both Mycoplasma in mycoides subsp. in mycoides SC ... [more ▼]

Three new insertion elements, IS Mbov1, ISMbov2 and ISMbov3, which are closely related to ISMag1 (Mycoplasma agalactiae), ISMmy1 and IS1634 (both Mycoplasma in mycoides subsp. in mycoides SC), respectively, have been discovered in Mycoplasma bovis, an important pathogen of cattle. Southern blotting showed that the genome of M. bovis harbours 6-12 copies of ISMbov1, 11-15 copies of ISMbov2 and 4-10 copies of ISMbov3, depending on the strain. A fourth insertion element, the IS30-like element, is present in 4-8 copies. This high number of IS elements in AI bovis, which represent a substantial part of its genome, and their relatedness with IS elements of both M. agalactiae and M. mycoides subsp. mycoides SC suggest the occurrence of two evolutionary events: (i) a divergent evolution into ill. agalactiae and M. bovis upon infection of different hosts; (ii) a horizontal transfer of IS elements during co-infection with M. mycoides subsp. mycoides SC and Ad. bovis of a same bovine host. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. [less ▲]

Detailed reference viewed: 22 (2 ULg)
See detailSpecific virulence properties of avian pathogenic Escherichia coli (APEC)
Mainil, Jacques ULg; Stordeur, P.; Moulin-Schouleur, M.

Conference (2005)

Detailed reference viewed: 1 (0 ULg)
Full Text
See detailRegulation of Virulence Gene Expression by “Quorum-sensing” - Science or Science-fiction ?
Mainil, Jacques ULg

in Annales de Médecine Vétérinaire (2005), 149C(Special issue), 33-40

Detailed reference viewed: 9 (0 ULg)
Full Text
See detailGenetics and Regulation of Bacterial Virulence - Towards the Molecular Version of Koch’s Postulates
Mainil, Jacques ULg

in Annales de Médecine Vétérinaire (2005), 149C(Special issue), 24-32

Detailed reference viewed: 4 (0 ULg)
Full Text
See detailDevelopment of Disease - Bacterial Toxins and their Interaction with Host Cells
Mainil, Jacques ULg

in Annales de Médecine Vétérinaire (2005), 149C(Special issue), 15-23

Detailed reference viewed: 4 (0 ULg)
Full Text
See detailColonisation of the Mucosae - Adherence Factors and their Interaction with Host Cells
Mainil, Jacques ULg

in Annales de Médecine Vétérinaire (2005), 149C(Special issue), 5-14

Detailed reference viewed: 5 (0 ULg)
Full Text
See detailMaladies infectieuses et micro-organismes : de la préhistoire aux postulats de Koch - grande et petite histoire
Mainil, Jacques ULg

in Annales de Médecine Vétérinaire (2005), 149C(Special issue), 3-4

Detailed reference viewed: 5 (0 ULg)
See detailDevelopment of disease : bacterial toxins and their interaction with host cells
Mainil, Jacques ULg

Scientific conference (2005)

Detailed reference viewed: 13 (2 ULg)
Full Text
See detailNecrotoxigenic Escherichia coli : study of the roles of CNF2 and CDT-III toxins in an experimental model of infection in calves
Mainil, Jacques ULg

in Annales de Médecine Vétérinaire (2005), 149(Sp. Iss. SI), 46-48

Detailed reference viewed: 11 (0 ULg)
Full Text
Peer Reviewed
See detailSynergistic action of E. coli endotoxin and Pasteurella multocida type A for the induction of bronchopneumonia in pigs
Halloy, David J.; Kirschvink, Nathalie A.; Mainil, Jacques ULg et al

in Veterinary Journal (2005), 169(3), 417-426

This study aimed to investigate whether Escherichia coli endotoxin (LPS) may predispose the lung to an infection with Pasteurella multocida type A (Pma) and to determine the LPS concentration needed to ... [more ▼]

This study aimed to investigate whether Escherichia coli endotoxin (LPS) may predispose the lung to an infection with Pasteurella multocida type A (Pma) and to determine the LPS concentration needed to reproduce clinical signs of bronchopneumonia. Twenty-four hours before inoculating Pma or sterile growth medium, piglets were tracheally instilled with 10, 100 or 400 microg/kg LPS. Cough, body temperature, daily weight gain (DWG) bronchoalveolar lavage fluid (BALF) cells and volume of pneumonic lung were measured. Changes in breathing pattern (Penh) were assessed by whole body barometric plethysmography. No significant changes were observed in Pma-treated or in control animals. Each LPS doses induced DWG reduction while the higher generated a severe subacute interstitial pneumonia causing hyperthermia and an increase in Penh. The combination of the lower LPS doses with Pma produced an asymptomatic bronchopneumonia leading to DWG reduction, rise in Penh and an increase in BALF macrophages and neutrophils. With 400 microg/kg LPS, Pma worsened the inflammatory process as illustrated by cough, hyperthermia, major DWG reduction and by a greater Penh response. Lung lesions consisted of severe exudative bronchopneumonia. We concluded that LPS may negatively influence growth, predispose to persisting lung inflammatory process and promote Pma infection depending on the dose previously administered. [less ▲]

Detailed reference viewed: 32 (15 ULg)