Magnetic solid-phase enzyme immunoassay for the detection of anti-glomerular basement membrane antibodies.
; ; Foidart, Jean-Michel et al
in Journal of Immunological Methods (1982), 48(2), 149-57
A new enzyme immunoassay has been developed for the demonstration of antiglomerular basement membrane antibodies. Magnetically responsive polyacrylamide-agarose beads (Magnogel) activated with ... [more ▼]
A new enzyme immunoassay has been developed for the demonstration of antiglomerular basement membrane antibodies. Magnetically responsive polyacrylamide-agarose beads (Magnogel) activated with glutaraldehyde were used to bind sonicated insoluble rat glomerular basement membranes. Both the collagenous and the non-collagenous moieties were demonstrated to be fixed on the beads. Sera from brown Norway rats with anti-glomerular basement membrane antibodies induced by HgCl2 injections were incubated with the beads. After washing, the fixed rat IgG were revealed using alkaline phosphatase labelled Fab fragments from anti-rat IgG sheep, IgGs. Comparison with a radioimmunoassay showed that results were reliable. This enzyme immunoassay has several advantages which may render this assay of considerable clinical usefulness. [less ▲]Detailed reference viewed: 20 (0 ULg)
Specificity of antibodies to heterologous glomerular and tubular basement membranes in various strains of mice with different H-2 types.
; ; et al
in Clinical & Experimental Immunology (1981), 46(1), 35-43
C3H, CBA (H-2k) and NZB (H-2d) mice were immunized with dog insoluble glomerular (GBM) or tubular basement membrane (TBM). The titre of circulating antibodies was sequentially determined and their ... [more ▼]
C3H, CBA (H-2k) and NZB (H-2d) mice were immunized with dog insoluble glomerular (GBM) or tubular basement membrane (TBM). The titre of circulating antibodies was sequentially determined and their specificity was analysed using various soluble antigenic fractions. Glomerular and tubular deposits were studied on serial biopsies by direct immunofluorescence. After elution from whole kidneys, IgG fixation on normal mouse kidney sections was analysed by indirect immunofluorescence. After immunization with insoluble GBM, animals from all three strains develop antibodies mainly directed against collagenous antigenic determinants shared by GBM and TBM. After immunization with insoluble TBM, the antibodies are directed in NZB mice against non-collagenous TBM-specific determinants, in C3H mice against collagenous determinants and in CBA mice against both types of antigenic determinants. Thus the ability to respond to the various antigens of GBM and TBM is genetically determined and does not depend only on the major histocompatibility complex. [less ▲]Detailed reference viewed: 9 (0 ULg)
Tissue culture of normal rat glomeruli. Basement membrane biosynthesis by homogeneous epithelial and mesangial cell lines.
; ; Foidart, Jean-Michel et al
in International Journal of Biochemistry (The) (1980), 12(1-2), 197-202Detailed reference viewed: 4 (0 ULg)