References of "Maghuin-Rogister, Guy"
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See detailReceptor-based screening assays: New perspectives in anti-doping control
Scippo, Marie-Louise ULg; Willemsen, Philippe; Danyi, Sophie ULg et al

in Chromatographia (2004), 59(Suppl. S), 23-27

The so-called "growth promoters", steroid hormones and beta-agonists, are currently controlled by using hyphenated analytical methods (chromatography coupled to mass spectrometry) or, sometimes for ... [more ▼]

The so-called "growth promoters", steroid hormones and beta-agonists, are currently controlled by using hyphenated analytical methods (chromatography coupled to mass spectrometry) or, sometimes for screening purposes, on immunoassays. These methods are often too specific to allow an effective multianalyte control. To develop more efficient assays, the use of hormonal receptors as detection tools (receptor-based binding assays and cell-based assays) is proposed. Receptor-based assays represent useful tools in screening of hormonal residues in food, but they could also be applied in doping control (to detect "new" hormonal substances). Furthermore, these assays could be used to monitor the human exposure to endocrine disruptors. [less ▲]

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See detailComparison of the rat and mouse cell lines commercially available for CALUX bioassays
Goeyens, Leo; Windal, Isabelle; Scippo, Marie-Louise ULg et al

in Organohalogen compounds (2004), 66

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See detailAssesment of the situation, three years later, on 'the Belgian Dioxin Crisis'. Probable impact on the health of the Belgian population
Focant, Jean-François ULg; Pirard, Catherine ULg; Douny, Caroline ULg et al

in Annales de Médecine Vétérinaire (2002), 146(6, DEC-JAN), 321-327

In January 1999, Belgium encounters an accidental contamination of the food chain, which was named "the Belgian Dioxin Crisis". The aim of the present review is to estimate the impact of this crisis on ... [more ▼]

In January 1999, Belgium encounters an accidental contamination of the food chain, which was named "the Belgian Dioxin Crisis". The aim of the present review is to estimate the impact of this crisis on public health. Currently, it does not really exist any exhaustive epidemiological study. Various scientific studies actually report somewhat conflicting results. As an example, concerning the population living in Wallonia, recorded dioxin levels are not higher than background levels reported for other industrialized countries, despite the 1999 "dioxin crisis". Conversely, based on levels measured in foodstuffs collected by the end of the crisis period, the dioxin body burden of potential consumers would have increased in the range of 40 to 75 %. The real impact of this crisis on consumers' health could thus only be estimated through well structured epidemiological campaigns. [less ▲]

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See detailUse of specific bioluminescent cell lines for the detection of steroid hormone (ant)agonists in meat producing animals
Willemsen, Philippe; Scippo, Marie-Louise ULg; Maghuin-Rogister, Guy ULg et al

in Analytica Chimica Acta (2002), 473(1-2), 119-126

We present stable transformed human mammary cell lines displaying highly inducible steroid receptor-mediated luciferase reporter gene expression. The utilization of a cell-based assay for detection of ... [more ▼]

We present stable transformed human mammary cell lines displaying highly inducible steroid receptor-mediated luciferase reporter gene expression. The utilization of a cell-based assay for detection of steroid agonists and antagonists down to 0.3 ng g(-1) is described and its application to the analysis of bovine samples is discussed. In particular, the use of the assays to detect illegal progesterone or androgen treatment has to take into account the presence of natural endogenous hormones. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailDetection of illegal growth promoters in biological samples using receptor binding assays
Scippo, Marie-Louise ULg; Van de Weerdt, Cécile ULg; Willemsen, Philippe et al

in Analytica Chimica Acta (2002), 473(1-2), 135-141

In the European Union (EU), the use of growth-promoting substances in meat production is banned. The control of growth promoters, especially steroid hormones, is presently based on expensive and time ... [more ▼]

In the European Union (EU), the use of growth-promoting substances in meat production is banned. The control of growth promoters, especially steroid hormones, is presently based on expensive and time-consuming chromatographic methods of analysis or, sometimes, for screening purposes, on radio- or enzyme-immunoassays, all of which are often too specific to allow effective multi-analyte control. In order to develop rapid and inexpensive multi-analyte detection tests, we proposed the use of hormonal receptors as detection tools. The system described here (radio-receptor assays) is based on a direct bindin g assay of steroid hormones to their respective receptors. Human receptors to estrogens (hERalpha), androgens (hAR), progestagens (hPR) and glucocorticoids (hGR) have been produced by genetic engineering in bacteria or in eucaryotic cells. Binding analyses revealed that the obtained receptor proteins retained a high affinity for their corresponding native ligand. In addition, competition studies continued that each of the four receptors displays a specificity profile for a series of analogs in agreement with the literature. Finally, the stability of these recombinant receptors is sufficient to allow their use in test kits. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailAdaptation to multiday ozone exposure is associated with a sustained increase of bronchoalveolar uric acid.
Kirschvink, Nathalie; Fievez, Laurence ULg; Bureau, Fabrice ULg et al

in Free Radical Research (2002), 36(1), 23-32

The phenomenon of ozone tolerance is described, but the underlying mechanisms remain unknown. We tested whether adaptation to multiday ozone exposure was related to an upregulated pulmonary antioxidant ... [more ▼]

The phenomenon of ozone tolerance is described, but the underlying mechanisms remain unknown. We tested whether adaptation to multiday ozone exposure was related to an upregulated pulmonary antioxidant defence. Six calves were exposed to 0.75 ppm ozone, 12 h day(-1) for seven consecutive days. Pulmonary function tests and bronchoalveolar lavage (BAL) were performed before, after the first (D1), third (D3) and seventh (D7) exposure. Differential cell count, total proteins, 8-epi-PGF2alpha, glutathione and uric acid were determined in BAL. Dynamic lung compliance and arterial oxygen tension were significantly decreased and lung oedema impaired pulmonary function on D1. By repeating ozone exposures, progressive functional adaptation occurred. Ozone induced a significant increase of BAL neutrophil percentage on D1. On D3 and D7, neutrophil percentage was progressively decreased, but remained significantly elevated. BAL total proteins were significantly increased on D1 and decreased progressively until D7. 8-Epi-PGF2alpha was significantly increased on D1 and was returned to baseline on D3 and D7, whilst glutathione significantly increased on D3 and returned to baseline on D7. Uric acid was increased ten-fold on D1. On D3, uric acid was increased six-fold and was persistently elevated at D7. This study suggests that ozone adaptation of functional and inflammatory variables is accompanied with sustained BAL uric acid elevation. [less ▲]

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See detailPrimary Extraction Technologies
Scippo, Marie-Louise ULg; Maghuin-Rogister, Guy ULg

in O'Keefe, Michael (Ed.) Residue Analysis in Food - Principles and Applications (2000)

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See detailReceptors as screening tools in the detections of hormones. Applications in the control of meat production
Maghuin-Rogister, Guy ULg; Baise, Etienne ULg; Carpeaux, Rudy et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (1999), 4(1), 21-22

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See detailLa pollution de la chaîne alimentaire par la dioxine
Maghuin-Rogister, Guy ULg; Delaunois, A.; De Pauwe, E. et al

in Annales de Médecine Vétérinaire (1999), 143

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See detailNew approach for the detection of growth promotors residues in animal products
Scippo, Marie-Louise ULg; Helbo, Vincent; Degand, Guy ULg et al

in Third meeting on animal productions. Biotechnologies: society stake. Proceedings of the study day held in Gembloux (Belgium) the 28 January 1998 (1998)

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See detailIdentification des vaches laitières traitées à la somatotropine bovine
Scippo, Marie-Louise ULg; Degand, Guy ULg; Duyckaerts, Anne et al

in Annales de Médecine Vétérinaire (1997), 141(5), 381-390

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See detailIdentification of the dairy cows treated with bovine somatotropine (BST): criteria to discriminate the treated animals from the non-treated ones
Scippo, Marie-Louise ULg; Degand, Guy ULg; Maghuin-Rogister, Guy ULg

in Second meeting on animal productions. The milk channel], Centre de Recherches Agronomiques, Gembloux (Belgium); Faculte Universitaire des Sciences Agronomiques de Gembloux (Belgium).- Gembloux (Belgium), 1997. p. Q3 (1997)

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See detailAntipeptide antibody against the bovine IGF-BP-2 : application to the detection of BST-treated cows
Scippo, Marie-Louise ULg; Degand, Guy ULg; Duyckaerts, Anne et al

in Food & Agricultural Immunology (1996), 8

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See detailAnalysis of the growth promoters residues in meat and offals and in the animals
Scippo, Marie-Louise ULg; Helbo, Vincent; Van Vyncht, Gery et al

in First meeting on animal productions. Beef production], Centre de Recherches Agronomiques, Gembloux (Belgium); Faculte Universitaire des Sciences Agronomiques de Gembloux (Belgium) (1996)

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See detailMethod of detection and surveillance of natural sex steroid hormones
Scippo, Marie-Louise ULg; Maghuin-Rogister, Guy ULg

in Proceedings of the Scientific Conference on Growth Promotion in Meat Production (1995)

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