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See detailReconstructed interfollicular feline epidermis as a model for Microsporum canis dermatophytosis
Tabart, Jérémy; Baldo, Aline ULg; Vermout, Sandy et al

in Journal of Medical Microbiology (2007), 56(7), 971-975

Microsporum canis is a pathogenic fungus that causes a superficial cutaneous infection called dermatophytosis. The complexity of mechanisms involved in dermatophytic infections makes relevant in vivo ... [more ▼]

Microsporum canis is a pathogenic fungus that causes a superficial cutaneous infection called dermatophytosis. The complexity of mechanisms involved in dermatophytic infections makes relevant in vivo studies particularly difficult to perform. The aim of this study was to develop a new in vitro model of M. canis dermatophytosis using feline fetal keratinocytes in reconstructed interfollicular epidermis, and to investigate its relevance in studying the host-pathogen relationship. Histological analysis of reconstructed interfollicular feline epidermis (RFE) revealed a fully differentiated epidermis. A proliferation assay showed replicating cells only in the basal layer, indicating that RFE is a well-stratified living tissue, leading to the formation of a horny layer. Histopathological analysis of RFE infected by M. canis arthroconidia revealed that the fungus invades the stratum corneum and produces SUB3, a keratinase implicated in the infectious process. In view of these results, an M. canis dermatophytosis model on RFE seems to be a useful tool to investigate mechanisms involved in natural M. canis feline infections. [less ▲]

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See detailEchinococcus multilocularis and Toxocara canis in urban red foxes (Vulpes vulpes) in Brussels, Belgium
Brochier, B.; De Blander, H.; Hanosset, R. et al

in Preventive Veterinary Medicine (2007), 80(1), 65-73

During the last decades, European red foxes (Vulpes vulpes) have been implicated in the transmission of several viral or parasitic pathogenic agents to domestic animals and humans. In urban areas, risks ... [more ▼]

During the last decades, European red foxes (Vulpes vulpes) have been implicated in the transmission of several viral or parasitic pathogenic agents to domestic animals and humans. In urban areas, risks of zoonoses transmission are likely to increase as a result of a higher rate of intra and inter-species contacts. Foxes occur on 35% of the Brussels-Capital Region area and local densities reach up to 4 family groups per square kilometre. According to the directive 2003/99/ECC, a first survey for the presence in foxes of Echinococcus multilocularis and Toxocara canis was conducted in Brussels from 2001 to 2004. None of 160 foxes were found to be infected with E. multilocularis and 24 of 134 foxes were found to be infected with T canis. Considering numbers of examined foxes, the sensitivity and the specificity of tests used for diagnosis, the 95% credibility intervals for the true prevalence of E. multilocularis and T canis were estimated in a Bayesian framework to be 0 to 1.87% (median value of 0%) and 12.7 to 26% (median value of 18.7%), respectively. For T canis, a significantly higher risk to be a carrier occurs in cubs and a significantly lower risk in adults. (c) 2007 Elsevier B.V. All rights reserved. [less ▲]

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See detailLes mécanismes d'adhérence des champignons responsables de mycoses superficielles
Baldo, Aline ULg; Mathy, Anne ULg; Vermout, S. et al

in Annales de Médecine Vétérinaire (2007), 151

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See detailBovine babesiosis in Belgium: preliminary results of a postal survey of veterinarians in 2006
Saegerman, Claude ULg; Claerebout, E.; Kalume, M. et al

in Rencontres autour des Recherches sur les Ruminants (2007), 14

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See detailDescription clinique des cas de FCO survenus au Nord de l'Europe durant l'été et l'automne 2006
Guyot, Hugues ULg; Mauroy, Axel ULg; Thiry, Etienne ULg et al

in Bulletin des Groupements Techniques Vétérinaires (2007), 39(avril), 89-96

La fièvre catarrhale ovine (FCO) a été identifiée au Nord de l’Europe <br />le 14 août 2006. L’Allemagne, la Belgique, les Pays-Bas et, dans une <br />moindre mesure, le Grand-Duché de Luxembourg et la ... [more ▼]

La fièvre catarrhale ovine (FCO) a été identifiée au Nord de l’Europe <br />le 14 août 2006. L’Allemagne, la Belgique, les Pays-Bas et, dans une <br />moindre mesure, le Grand-Duché de Luxembourg et la France ont été <br />affectés. Le sérotype 8 du virus de la FCO (exotique) a rapidement été <br />identifié ainsi qu’un vecteur, Culicoides dewulfi, une espèce indigène du <br />Nord de l’Europe. La maladie s’est rapidement développée et au 1er <br />février 2007, on recensait 2137 foyers de FCO, toutes espèces de <br />ruminants confondues. Globalement, la maladie a affecté davantage les <br />bovins (54 %) que les ovins (46 %). Les signes cliniques les plus <br />fréquemment observés chez les bovins étaient des lésions sur le museau <br />et la cavité buccale (ulcérations/croûtes), de la salivation et des boiteries <br />chez des animaux adultes. La morbidité et la mortalité animales étaient <br />respectivement de maximum 5 % et 1 %. Les principaux signes cliniques <br />rencontrés chez le mouton étaient également des lésions sur le museau <br />et la cavité buccale (ulcérations/croûtes), de l’amaigrissement et des <br />boiteries. La morbidité et la mortalité animales étaient respectivement <br />de maximum 12 % et 6 %. Les signes cliniques n’étant pas <br />pathognomoniques et le diagnostic différentiel relativement vaste, seul <br />un examen de laboratoire permet d’établir un diagnostic de certitude. [less ▲]

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See detailBiting Midges Overwintering In Belgium
Losson, Bertrand ULg; Mignon, Bernard ULg; Paternostre, Julien ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2007), 160(13), 451-452

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See detailReconstructed interfollicular feline epidermis as a model for the screening of drugs against Microsporum canis
Mignon, Bernard ULg; Tabart, J.; Baldo, Aline ULg et al

in Veterinary Dermatology (2007), 18

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See detailDiagnostic et surveillance épidémiologique de Neospora caninum
Ghalmi, F.; China, B.; Losson, Bertrand ULg

in Annales de Médecine Vétérinaire (2007), 151

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See detailFièvre Catarrhale Ovine chez les ruminants. Description clinique des cas vécus dans le Nord de l’Europe durant l’été-automne 2006
Guyot, Hugues ULg; Mauroy, Axel ULg; Thiry, Etienne ULg et al

in Bulletin des GTV (2007)

La fièvre catarrhale ovine (FCO) a été identifiée au Nord de l’Europe le 14 août 2006. L’Allemagne, la Belgique, les Pays-Bas et, dans une moindre mesure, le Grand-Duché de Luxembourg et la France ont été ... [more ▼]

La fièvre catarrhale ovine (FCO) a été identifiée au Nord de l’Europe le 14 août 2006. L’Allemagne, la Belgique, les Pays-Bas et, dans une moindre mesure, le Grand-Duché de Luxembourg et la France ont été affectés. Le sérotype 8 du virus de la FCO (exotique) a rapidement été identifié ainsi qu’un vecteur, Culicoides dewulfi, une espèce indigène du Nord de l’Europe. La maladie s’est rapidement développée et au 1er février 2007, on recensait 2137 foyers de FCO, toutes espèces de ruminants confondues. Globalement, la maladie a affecté davantage les bovins (54 %) que les ovins (46 %). Les signes cliniques le plus fréquemment observés chez les bovins étaient des lésions sur le museau et la cavité buccale (ulcérations/croûtes), de la salivation et des boiteries chez des animaux adultes. La morbidité et la mortalité animales étaient respectivement de maximum 5 % et 1 %. Les principaux signes cliniques rencontrés chez le mouton étaient également des lésions sur le museau et la cavité buccale (ulcérations/croûtes), de l’amaigrissement et des boiteries. La morbidité et la mortalité animales étaient respectivement de maximum 12 % et 6 %. Les signes cliniques n’étant pas pathognomoniques et le diagnostic différentiel relativement vaste, seul un examen de laboratoire permet de poser un diagnostic de certitude. [less ▲]

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See detailSpatial spreading of Echinococcus multilocularis in Red foxes (Vulpes vulpes) across nation borders in Western Europe
Vervaeke, M.; van der Giessen, J.; Brochier, B. et al

in Preventive Veterinary Medicine (2006), 76(3-4), 137-150

The occurrence of the fox tapeworm Echinococcus multilocularis in Red foxes was studied in Belgium and a neighbouring region in The Netherlands. A total number of 1202 foxes were analysed (1018 in Belgium ... [more ▼]

The occurrence of the fox tapeworm Echinococcus multilocularis in Red foxes was studied in Belgium and a neighbouring region in The Netherlands. A total number of 1202 foxes were analysed (1018 in Belgium and 184 in The Netherlands) of which 179 were infected with E. multilocularis (164 in Belgium and 15 in The Netherlands). Further, the spatial distribution of infection among sampled foxes was analysed with an ellipsoidal gradient, demonstrating a decreasing prevalence in northwestern direction. Using this gradient, we showed that the spatial patterns of infection in Belgium and the neighbouring region in The Netherlands correspond, indicating a continuous distribution of E. multilocularis across the nation borders. Part of the Belgian data allowed investigating temporal changes in the spatial distribution of E. multilocularis. This revealed a northwestern spread of E. multilocularis. (c) 2006 Elsevier B.V. All rights reserved. [less ▲]

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See detailEcological characteristic's of small farmland ponds: Associations with land use practices at multiple spatial scales
Declerck, Steven; De Bie, Tom; Ercken, Dirk et al

in Biological Conservation (2006), 131(4), 523-532

Despite their restricted surface area, small farmland ponds often have a high conservation value because they contribute significantly to regional biodiversity and contain rare or unique species. For this ... [more ▼]

Despite their restricted surface area, small farmland ponds often have a high conservation value because they contribute significantly to regional biodiversity and contain rare or unique species. For this reason, the creation of new ponds has become a widely applied practice in many countries. information on the effects of land use on farmland ponds is very scarce. Farmland ponds differ from larger ponds, lakes and livers in many aspects and can therefore be expected to be affected by land use via other mechanisms operating at different spatial scales. We here present a study on 126 ponds distributed over the entire territory of Belgium (surface area: 30.500 km(2)). We assessed variables related to turbidity state and vegetation complexity and related them to land use variables assessed at several spatial scales ranging from the pond edge up to 32 km(2) circular areas. According to redundancy analysis, trampling by cattle and percentage cover of nearby crop land were positively associated with turbid state related variables. Conversely, ponds with high coverage by forest in the immediate neighbourhood tended to be more associated with the clear water state. Multiple regression analysis demonstrated a negative effect of trampling and coverage by crop land on vegetation complexity. Effects of crop lands and forest were strongest at the local scale (< 200 m radius) which indicates that adverse external influences can most efficiently be mitigated at a small scale. Based on these results we suggest several recommendations for pond construction and conservation. (c) 2006 Elsevier Ltd. All rights reserved. [less ▲]

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See detailIn vitro entomopathogenic activity of Beauveria bassiana against Psoroptes spp. (Acari : Psoroptidae)
Lekimme, Mireille ULg; Mignon, Bernard ULg; Tombeux, S. et al

in Veterinary Parasitology (2006), 139(1-3), 196-202

An indigenous strain (IHEM 18747) of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycetes) was evaluated for its in vitro entomopathogenic activity against the parasitic mite Psoroptes ovis Hering ... [more ▼]

An indigenous strain (IHEM 18747) of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycetes) was evaluated for its in vitro entomopathogenic activity against the parasitic mite Psoroptes ovis Hering (Acari: Psoroptidae) from rabbits. The following aspects were evaluated: (1) effects of conidial concentration on the viability of adult females; (2) influence of the infection on the fertility, and on the hatchability of eggs; (3) and transmission of infection between mites, and from contaminated surface. Adult females immersed into increasing concentrations of conidia (10(4)-10(9) conidia ml(-1)) showed a dose-related susceptibility. At the highest concentration of conidia, LT50 was 1.6 days while LT50 of the controls reached 5.8 days. The fungus was able to sporulate on the body surface and 100% of the mites were covered with mycelium after immersion in solutions containing 10(7)-10(9) conidia ml(-1). One hundred percent of healthy mites exposed to infected cadavers or surfaces acquired the infection (LT50 reached 1.9 and 1.73 days, respectively, versus 6.1 and 5.1 days in controls, respectively). Egg laying was not reduced by the fungal infection but both the hatchability of the eggs and the life span of the emerging larvae were significantly reduced. Eggs directly infected with the fungus did not show reduced hatchability but the life span of the larvae was shortened. It is concluded that B. bassiana has a high entomopathogenic activity against Psoroptes spp. The in vivo use of this biocontrol agent against Psoroptes spp. in rabbit, sheep and cattle deserves further attention. (c) 2006 Elsevier B.V. All rights reserved. [less ▲]

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See detailIn vitro tests for evaluation of the hatchability of the eggs of Psoroptes mites following exposure to acaricidal compounds.
Lekimme, Mireille ULg; Mignon, Bernard ULg; Leclipteux, Thierry ULg et al

in Medical and Veterinary Entomology (2006), 20(1), 102-105

Three in vitro assays for the evaluation of the hatchability of the eggs of the mite Psoroptes ovis (Hering) (Acari: Psoroptidae) are described. Preliminary trials showed that hatching occurs at very high ... [more ▼]

Three in vitro assays for the evaluation of the hatchability of the eggs of the mite Psoroptes ovis (Hering) (Acari: Psoroptidae) are described. Preliminary trials showed that hatching occurs at very high rates when eggs are incubated under conditions of high humidity, on a liquid medium and in agarose dishes. These three protocols were compared, taking into account the ease of preparation, follow-up and accuracy of counting. The best protocol was found to be the use of agarose dishes. It was accurate, easy to carry out and reproducible for further evaluation of existing or potentially new compounds against both adults and eggs of Psoroptes spp. The acaricidal properties of phoxim and amitraz were then evaluated against eggs and adults using the three protocols. Results showed that for both drugs, in vitro adulticidal activity was complete, whereas the in vitro ovicidal activity was only partial. Nevertheless, efficacy of amitraz against both adults and eggs was shown to be higher than that of phoxim. [less ▲]

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See detailEvaluation des risques liés à la présence d’Echinococcus multilocularis et de Toxocara canis dans la population vulpine en région bruxelloise
Saegerman, Claude ULg; De Blander, H.; Hanosset, R. et al

in Epidémiologie et Santé Animale (2006), 50

During the last decades, European red foxes (Vulpes vulpes) have been implicated in the transmission of several viral or parasitic pathogenic agents to domestic animals and humans. In urban areas, risks ... [more ▼]

During the last decades, European red foxes (Vulpes vulpes) have been implicated in the transmission of several viral or parasitic pathogenic agents to domestic animals and humans. In urban areas, risks of zoonoses transmission are likely to increase as a result of a higher rate of intra- and inter-species contacts. Foxes occur on 35% of the Brussels-Capital Region area and local densities reach up to 4 family groups per km². According to the directive 2003/99/ECC, a first survey for thepresence in foxes of Echinococcus multilocularis and Toxocara canis was conducted in Brussels from 2001 to 2004. None of 160 foxes were found to be infected with Echinococcus multilocularis and 24 of 134 foxes were found to be infected with Toxocara canis. Considering numbers of examined foxes, the sensitivity and the specificity of tests used for diagnosis, the 95% credibility intervals for the true prevalence of carriage of Echinococcus multilocularis and Toxocara canis were estimated in a Bayesian framework to be 0% to 1.87% (median value of 0%) and 12.7% to 26% (median value of 18.7.%) respectively. For Toxocara canis, a significantly higher risk to be a carrier occurs in cubs and a significantly lower risk in adults. [less ▲]

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See detailKinetics of Microsporum canis adherence using an in vitro model of reconstituted feline epidermis
Baldo, Aline ULg; Tabart, J.; Vermout, S. et al

Conference (2006)

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See detailRecent findings on the pathogenesis of dermatophytoses in dogs and cats
Mignon, Bernard ULg; Vermout, S.; Tabart, J. et al

in Revista Scientia Parasitologica (2006), 7(3/4), 7-15

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See detailTwo Microsporum canis dipeptidyl peptidase genes with possible involvement in fungal virulence
Vermout, S.; Baldo, Aline ULg; Tabart, J. et al

Conference (2006)

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See detailA new in vitro model of Microsporum canis dermatophytosis in reconstituted feline skin
Tabart, J.; Baldo, Aline ULg; Vermout, S. et al

Conference (2006)

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