References of "Losson, Bertrand"
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See detailThree cases of Parafilaria bovicola infection in Belgium, and a few recent epidemiological observations on this emergent disease
Caron, Yannick ULg; Groignet, Stéphanie; Saegerman, Claude ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2013), 175

Parafilariosis is a vector borne parasitic disease caused by the development of the nematode Parafilaria bovicola in the subcutaneous and intermuscular connective tissues of cattle. On February 28th 2012 ... [more ▼]

Parafilariosis is a vector borne parasitic disease caused by the development of the nematode Parafilaria bovicola in the subcutaneous and intermuscular connective tissues of cattle. On February 28th 2012, the so-called bleeding spots were observed in two heifers and one bull in a cattle herd close to Namur (Belgium). The animals had been treated in December with an injectable ivermectin/closantel solution (Closamectin pour on®, Norbrook Lab) at the recommended dosage. Samples of serohaemorrhagic exudate and blood as well as skin biopsies were collected. Embryonated eggs of Parafilaria bovicola in the serohaemorrhagic exudate and high levels of creatine phosphokinase (CPK) were detected. Clinically affected animals were treated with injectable ivermectin (Ivomec®, Merial) at 200 µg/kg. Two epidemiological phone surveys were carried out in the south of Belgium (Wallonia) in order to estimate the geographical distribution of this condition since it was first described and published in 2009. A standardized questionnaire was used and the results were analysed. Most outbreaks were recorded in the provinces of Liege and Luxembourg. The initial source of infection is still unknown but this parasitic infection is clearly spreading from the initial Belgian outbreak site. [less ▲]

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See detailThelazia callipaeda ocular infection in two dogs in Belgium
Caron, Yannick ULg; Prémont, Johanna ULg; Losson, Bertrand ULg et al

in Journal of Small Animal Practice (2013), 54(3),

Eyeworms were retrieved from the left eyes of two dogs presented for unilateral ocular discharge in Belgium. Morphological and molecular identification were performed and the parasites were identified as ... [more ▼]

Eyeworms were retrieved from the left eyes of two dogs presented for unilateral ocular discharge in Belgium. Morphological and molecular identification were performed and the parasites were identified as Thelazia callipaeda. The history suggested that the infection had been acquired in South-Western France and Southern Italy where the disease has been observed regularly for the last 6 and 12 years, respectively. In these two regions, the disease is considered endemic and spreading. To the best of our knowledge, this is the first complete case report of canine thelaziosis in Belgium. The risk of introduction of the parasite in Northern Europe and particularly in Belgium is discussed. [less ▲]

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See detailZoonoses in Pet 1 birds: review and perspectives
Boseret, Géraldine ULg; Losson, Bertrand ULg; Mainil, Jacques ULg et al

in Veterinary Research (2013)

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See detailChemical composition of silage residues sustaining the larval development of the Culicoides obsoletus/Culicoides scoticus species (Diptera: Ceratopogonidae)
Zimmer, Jean-Yves ULg; Saegerman, Claude ULg; Losson, Bertrand ULg et al

in Veterinary Parasitology (2013), 191(1-2), 197-201

Culicoides (Diptera: Ceratopogonidae) are biological vectors of bluetongue virus (BTV). Bluetongue is a viral disease that affects domestic and wild ruminants. Since its recent emergence in northern ... [more ▼]

Culicoides (Diptera: Ceratopogonidae) are biological vectors of bluetongue virus (BTV). Bluetongue is a viral disease that affects domestic and wild ruminants. Since its recent emergence in northern Europe, this disease has caused considerable economic losses to the sheep and cattle industry. The biotopes, and more particularly the chemical characteristics which are suitable for larval development of the main vector species, are still relatively unknown. This study shows that the larvae of biting midges belonging to the species Culicoides obsoletus and Culicoides scoticus are able to breed in different types of silage residue (maize, grass, sugar beet pulp and their combinations). The chemical composition of substrates strongly influences the presence of the immature stages of these biting midges. Higher lignin and insoluble fibre contents seem to favour their presence and could play the role of a physical support for semi-aquatic larvae. In contrast, higher concentrations of magnesium and calcium are negatively correlated with the presence of these two species. These data will help to locate and monitor the breeding sites of these species and could contribute to the control of these insects on farms. [less ▲]

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See detailSeasonal change of Lymnaeid snails intermediate host of Fasciola gigantica in North and Central Vietnam
Bui Thi, Dung ULg; Dang Tat, The; Pham Ngoc, Doanh et al

Conference (2012, December)

Objective: The present study was undertaken to investigate the population dynamics of the intermediate hosts of Fasciola gigantica and the levels of infection in the snails. This would allow identifying ... [more ▼]

Objective: The present study was undertaken to investigate the population dynamics of the intermediate hosts of Fasciola gigantica and the levels of infection in the snails. This would allow identifying the most important transmission periods and suggesting optimal snail control for the area. Methods: Lymnaeid snails were monthly collected by hand picking in Ha Noi (Northern Vietnam) during the period from March 2010 to December 2011 and in Binh Dinh province (Central Vietnam) during the period May and September 2012. Collected snails from different sites were identified based on morphology and the partial 16S rDNA sequence and ITS-2 sequence. Snails were examined for the presence of Fasciola larval by the crushing method and confirmation by multiplex PCR analyses with PCR primer for Fasciola gigantica cathepsin L in parallel with the snail rDNA species specific primer. Result: The density of Lymnaeid populations underwent great changes in relation to the geographical locations and seasons. In Central Vietnam, the Lymnaeid populations reached the peak in the dry/rainy season (May) and decreased sharply in rainy/dry season (September). In contrast, in Hanoi, it reached the peaks in 2 periods (February to April and August to November) of rice cultivation or early stage of growing of rice, and greatly deceased when the rice becomes fully developed. Transmission of fascioliasis in Ha Noi were high when rice cultivation is performed, while the permanent transmission can takes place through the year in Binh Dinh with peak transmission during the dry season. [less ▲]

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See detailCulicoides (Diptera : Ceratopogonidae) : important vectors of cattle diseases. Control assays in Belgium
Smeets, François ULg; Robert, Nancy; Simonon, Grégory et al

Poster (2012, October 19)

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See detailBreeding sites of main Bluetongue virus vectors in Belgian cowshed
Zimmer, Jean-Yves ULg; Saegerman, Claude ULg; Losson, Bertrand ULg et al

Poster (2012, August)

Bluetongue (BT) is an emerging vectorborne disease of ruminants that was reported in August 2006 in northern Europe. Since 2007, BT virus (BTV) serotype 8 continued its spread across Europe and caused ... [more ▼]

Bluetongue (BT) is an emerging vectorborne disease of ruminants that was reported in August 2006 in northern Europe. Since 2007, BT virus (BTV) serotype 8 continued its spread across Europe and caused considerable economic losses. This observation indicates possible overwintering of the vector from year to year. The biological vectors of BTV are biting midges of the genus Culicoides. Breeding sites of bluetongue vector species have been found near farms (e.g. silage residues) and in neighboring meadows (e.g. cattle dung) but never inside sheds. We conducted a study in five cattle farms in Belgium during February–October 2008. Three samplings were performed and each soil sample collected inside cowsheds was incubated to enable adult midges to emerge. Among 15 soil biotopes sampled, only one showed the emergence of adult Culicoides biting midges: dried dung adhering to walls inside animal enclosures and resulting to the partial removal of used animal litter. It was a breeding site for the C. obsoletus/C. scoticus complex. Physico-chemical characteristics showed that midges of this complex are more prevalent in soil samples with a high carbon:nitrogen (C:N) index. So Culicoides biting midges are able to complete their life cycle in animal enclosures. We identified a breeding site for the primary BTV vector in a cowshed in northern Europe. These observations could explain the persistence of BTV from year to year despite fairly harsh winters. Hygienic measures on farms could reduce biting midges populations and so improve efficacy of vaccination campaigns against BT in Europe. [less ▲]

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See detailParásitos en primates de la Amazonía Ecuatoriana: una herramienta para la salud pública y la conservación
Martin, Sarah ULg; Carrillo-Bilbao, Gabriel Alberto; Celi, Maritza et al

Conference (2012, June 22)

Los primates son reservorios de patógenos humanos ya sea que estén en libertad o en cautiverio. Si identificamos los parásitos y las enfermedades que estos ocasionan en primates, sería una herramienta ... [more ▼]

Los primates son reservorios de patógenos humanos ya sea que estén en libertad o en cautiverio. Si identificamos los parásitos y las enfermedades que estos ocasionan en primates, sería una herramienta para su conservación y un aporte significativo para la salud pública. Colectamos e identificamos los parásitos gastrointestinales de 10 especies de primates de la Amazonía ecuatoriana. Se utilizo la técnica de flotación con solución de azúcar sobre saturada (d≈1.28 - 1.33) y la técnica de Ritchie. Las muestras fueron analizadas según factores extrínsecos e intrínsecos de los primates. Los valores generales de prevalencia para protozoarios y helmintos fueron de 17.6% y 55.4% respectivamente. Infecciones con un solo parásito fueron observadas en 46% de las muestras e infecciones con dos o más parásitos fueron observadas en tan solo 17.6% de las muestras. Las hembras tuvieron mayor prevalencia (93.33%) que los machos (68.75%). Más de la mitad de los parásitos encontrados en este estudio (Necator/Ancylostoma, Capillaria sp., Strongylus sp., Entamoeba histolytica, Hymenolepis sp., Oesophagostomum sp.) son una amenaza potencial de transmisión zoonótica. Lagothrix lagotricha tiene la mayor diversidad de parásitos (7) en comparación con las otras especies de primates estudiadas. Este estudio muestra una diversidad de parásitos de importancia zoonótica, lo cual demuestra el interés que deber presentarse tanto para el manejo de fauna silvestre como para programas de salud pública. [less ▲]

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See detailLongitudinal field study on bovine Babesia spp. and Anaplasma phagocytophilum infections during a grazing season in Belgium
Lempereur, Laetitia ULg; Lebrun, Maude; Cuvelier, Pascale et al

in Parasitology Research (2012), 110(4), 1525-1530

Anaplasmosis and babesiosis are major tick-borne diseases with a high economic impact but are also a public health concern. Blood samples collected in the spring, summer, and autumn of 2010 from 65 cows ... [more ▼]

Anaplasmosis and babesiosis are major tick-borne diseases with a high economic impact but are also a public health concern. Blood samples collected in the spring, summer, and autumn of 2010 from 65 cows in seven different farms in Belgium were monitored with an indirect immunofluorescence antibody test to assess seroprevalence against these pathogens. Seroprevalences to Babesia spp. were measured as 10.7%, 20%, and 12.3% in spring, summer, and autumn, respectively, whereas seroprevalences to Anaplasma phagocytophilum were 30.8%, 77%, and 56.9%, respectively. A total of 805 Ixodes ricinus ticks were collected at the same time from both cattle (feeding ticks) and grazed pastures (questing ticks). The infection level of ticks, assessed by PCR assay, for Babesia spp. DNA was 14.6% and 7.9% in feeding and questing ticks, respectively, whereas 21.7% and 3% of feeding and questing ticks were found be positive for A. phagocytophilum cDNA. Fifty-five PCR-positive samples were identified by sequencing as Babesia sp. EU1, of which five from feeding ticks were positive for both A. phagocytophilum and Babesia sp. EU1. The high density of wild cervids in the study area could explain these observations, as deer are considered to be the main hosts for adults of I. ricinus. However, the absence of Babesia divergens both in feeding and questing ticks is surprising, as the study area is known to be endemic for cattle babesiosis. Increasing cervid populations and comorbidity could play an import role in the epidemiology of these tick-borne diseases. [less ▲]

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See detailLes Culicoides, importants vecteurs de maladies du bétail
Smeets, François ULg; Robert, Nancy; Simonon, Grégory et al

Diverse speeche and writing (2012)

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See detailRotenoid content and in vitro acaricidal activity of Tephrosia vogelii leaf extract on the tick Rhipicephalus appendiculatus
Kalume; Losson, Bertrand ULg; Angenot, Luc ULg et al

in Veterinary Parasitology (2012), 190(1-2), 204-209

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See detailFoci report on indigenous Dermacentor reticulatus populations in Belgium and a preliminary study of associated babesiosis pathogens.
Cochez, C.; Lempereur, Laetitia ULg; Madder, Maxime et al

in Medical and Veterinary Entomology (2012), 26(3), 355-358

The occurrence of autochthonous clinical cases of canine and equine babesiosis in Belgium during the last two decades suggests that the vector of the pathogens responsible for these diseases, Dermacentor ... [more ▼]

The occurrence of autochthonous clinical cases of canine and equine babesiosis in Belgium during the last two decades suggests that the vector of the pathogens responsible for these diseases, Dermacentor reticulatus (Ixodida: Ixodidae), may be present in this country. Consequently, evidence for the presence of this tick species in different locations within Belgium was investigated. Four different locations were monitored by flagging in 2010; these included the locations at which D. reticulatus was previously found on a dog in 2009 and on two red deer in 2007. Two different species of tick were identified, Ixodes ricinus (Ixodida: Ixodidae) and D. reticulatus. A total of 282 D. reticulatus adult ticks (98 males, 184 females) were collected from the four sites. Ticks were found mainly from early March until the end of May and a peak in activity was apparent in March. A Babesia spp. (Piroplasmida: Babesiidae) genus-specific polymerase chain reaction test based on the amplification of a fragment of the 18S rRNA gene was used to investigate the potential presence of Babesia spp. All DNA extracts isolated from the total tick samples yielded negative results. Additional studies to accurately determine the distribution and vectorial capacity of this important tick species in Belgium are warranted. [less ▲]

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See detailWild Cervids Are Host for Tick Vectors of Babesia Species with Zoonotic Capability in Belgium
Lempereur, Laetitia ULg; Wirtgen, Marc ULg; Nahayo, Adrien ULg et al

in Vector Borne & Zoonotic Diseases (2012), 12(4), 275-280

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See detailAn optimized DNA extraction and multiplex PCR for the detection of Fasciola sp. in lymnaeid snails
Caron, Yannick ULg; Righi, Souad; Lempereur, Laetitia ULg et al

in Veterinary Parasitology (2011), 178((1-2)), 93-9

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See detailTreatment of a case of trichurosis (Trichuris suis) in pigs
Caron, Yannick ULg; Delleur, Valery; De Bock, Bart et al

Poster (2011, May)

Trichuris suis infection was identified in a pig herd during an investigation into persistent diarrhea and weight loss in 10 recently introduced gilts. Eggs of the parasite were identified in faeces of ... [more ▼]

Trichuris suis infection was identified in a pig herd during an investigation into persistent diarrhea and weight loss in 10 recently introduced gilts. Eggs of the parasite were identified in faeces of gilts. Further investigations failed to detect other causes of diarrhea but, in all production stages, they allowed the detection of eggs of Trichuris suis that could explain pasty faeces sometimes observed in weaned sows and frequent diarrhea found in the early fattening period. The administration of levamisole (intramuscular injection) to gilts allowed the excretion of adult parasites, the treatment of diarrhea and the reduction of the number of eggs per gram of faeces. Taking into account the success obtained with levamisole in gilts, the equipment of the farm and the wish of the farmer no to inject finishing pigs, an unsuccessful treatment was tried based on levamisole in drinking water every 8 and then 5 weeks. Flubendazole was finally administrated in drinking water every 3 and then 5 weeks, reducing significantly the number of eggs per gram of faeces. [less ▲]

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