References of "Losson, Bertrand"
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See detailSpatial spreading of Echinococcus multilocularis in Red foxes (Vulpes vulpes) across nation borders in Western Europe
Vervaeke, M.; van der Giessen, J.; Brochier, B. et al

in Preventive Veterinary Medicine (2006), 76(3-4), 137-150

The occurrence of the fox tapeworm Echinococcus multilocularis in Red foxes was studied in Belgium and a neighbouring region in The Netherlands. A total number of 1202 foxes were analysed (1018 in Belgium ... [more ▼]

The occurrence of the fox tapeworm Echinococcus multilocularis in Red foxes was studied in Belgium and a neighbouring region in The Netherlands. A total number of 1202 foxes were analysed (1018 in Belgium and 184 in The Netherlands) of which 179 were infected with E. multilocularis (164 in Belgium and 15 in The Netherlands). Further, the spatial distribution of infection among sampled foxes was analysed with an ellipsoidal gradient, demonstrating a decreasing prevalence in northwestern direction. Using this gradient, we showed that the spatial patterns of infection in Belgium and the neighbouring region in The Netherlands correspond, indicating a continuous distribution of E. multilocularis across the nation borders. Part of the Belgian data allowed investigating temporal changes in the spatial distribution of E. multilocularis. This revealed a northwestern spread of E. multilocularis. (c) 2006 Elsevier B.V. All rights reserved. [less ▲]

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See detailEcological characteristic's of small farmland ponds: Associations with land use practices at multiple spatial scales
Declerck, Steven; De Bie, Tom; Ercken, Dirk et al

in Biological Conservation (2006), 131(4), 523-532

Despite their restricted surface area, small farmland ponds often have a high conservation value because they contribute significantly to regional biodiversity and contain rare or unique species. For this ... [more ▼]

Despite their restricted surface area, small farmland ponds often have a high conservation value because they contribute significantly to regional biodiversity and contain rare or unique species. For this reason, the creation of new ponds has become a widely applied practice in many countries. information on the effects of land use on farmland ponds is very scarce. Farmland ponds differ from larger ponds, lakes and livers in many aspects and can therefore be expected to be affected by land use via other mechanisms operating at different spatial scales. We here present a study on 126 ponds distributed over the entire territory of Belgium (surface area: 30.500 km(2)). We assessed variables related to turbidity state and vegetation complexity and related them to land use variables assessed at several spatial scales ranging from the pond edge up to 32 km(2) circular areas. According to redundancy analysis, trampling by cattle and percentage cover of nearby crop land were positively associated with turbid state related variables. Conversely, ponds with high coverage by forest in the immediate neighbourhood tended to be more associated with the clear water state. Multiple regression analysis demonstrated a negative effect of trampling and coverage by crop land on vegetation complexity. Effects of crop lands and forest were strongest at the local scale (< 200 m radius) which indicates that adverse external influences can most efficiently be mitigated at a small scale. Based on these results we suggest several recommendations for pond construction and conservation. (c) 2006 Elsevier Ltd. All rights reserved. [less ▲]

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See detailIn vitro entomopathogenic activity of Beauveria bassiana against Psoroptes spp. (Acari : Psoroptidae)
Lekimme, Mireille ULg; Mignon, Bernard ULg; Tombeux, S. et al

in Veterinary Parasitology (2006), 139(1-3), 196-202

An indigenous strain (IHEM 18747) of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycetes) was evaluated for its in vitro entomopathogenic activity against the parasitic mite Psoroptes ovis Hering ... [more ▼]

An indigenous strain (IHEM 18747) of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycetes) was evaluated for its in vitro entomopathogenic activity against the parasitic mite Psoroptes ovis Hering (Acari: Psoroptidae) from rabbits. The following aspects were evaluated: (1) effects of conidial concentration on the viability of adult females; (2) influence of the infection on the fertility, and on the hatchability of eggs; (3) and transmission of infection between mites, and from contaminated surface. Adult females immersed into increasing concentrations of conidia (10(4)-10(9) conidia ml(-1)) showed a dose-related susceptibility. At the highest concentration of conidia, LT50 was 1.6 days while LT50 of the controls reached 5.8 days. The fungus was able to sporulate on the body surface and 100% of the mites were covered with mycelium after immersion in solutions containing 10(7)-10(9) conidia ml(-1). One hundred percent of healthy mites exposed to infected cadavers or surfaces acquired the infection (LT50 reached 1.9 and 1.73 days, respectively, versus 6.1 and 5.1 days in controls, respectively). Egg laying was not reduced by the fungal infection but both the hatchability of the eggs and the life span of the emerging larvae were significantly reduced. Eggs directly infected with the fungus did not show reduced hatchability but the life span of the larvae was shortened. It is concluded that B. bassiana has a high entomopathogenic activity against Psoroptes spp. The in vivo use of this biocontrol agent against Psoroptes spp. in rabbit, sheep and cattle deserves further attention. (c) 2006 Elsevier B.V. All rights reserved. [less ▲]

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See detailIn vitro tests for evaluation of the hatchability of the eggs of Psoroptes mites following exposure to acaricidal compounds.
Lekimme, Mireille ULg; Mignon, Bernard ULg; Leclipteux, Thierry ULg et al

in Medical and Veterinary Entomology (2006), 20(1), 102-105

Three in vitro assays for the evaluation of the hatchability of the eggs of the mite Psoroptes ovis (Hering) (Acari: Psoroptidae) are described. Preliminary trials showed that hatching occurs at very high ... [more ▼]

Three in vitro assays for the evaluation of the hatchability of the eggs of the mite Psoroptes ovis (Hering) (Acari: Psoroptidae) are described. Preliminary trials showed that hatching occurs at very high rates when eggs are incubated under conditions of high humidity, on a liquid medium and in agarose dishes. These three protocols were compared, taking into account the ease of preparation, follow-up and accuracy of counting. The best protocol was found to be the use of agarose dishes. It was accurate, easy to carry out and reproducible for further evaluation of existing or potentially new compounds against both adults and eggs of Psoroptes spp. The acaricidal properties of phoxim and amitraz were then evaluated against eggs and adults using the three protocols. Results showed that for both drugs, in vitro adulticidal activity was complete, whereas the in vitro ovicidal activity was only partial. Nevertheless, efficacy of amitraz against both adults and eggs was shown to be higher than that of phoxim. [less ▲]

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See detailEvaluation des risques liés à la présence d’Echinococcus multilocularis et de Toxocara canis dans la population vulpine en région bruxelloise
Saegerman, Claude ULg; De Blander, H.; Hanosset, R. et al

in Epidémiologie et Santé Animale (2006), 50

During the last decades, European red foxes (Vulpes vulpes) have been implicated in the transmission of several viral or parasitic pathogenic agents to domestic animals and humans. In urban areas, risks ... [more ▼]

During the last decades, European red foxes (Vulpes vulpes) have been implicated in the transmission of several viral or parasitic pathogenic agents to domestic animals and humans. In urban areas, risks of zoonoses transmission are likely to increase as a result of a higher rate of intra- and inter-species contacts. Foxes occur on 35% of the Brussels-Capital Region area and local densities reach up to 4 family groups per km². According to the directive 2003/99/ECC, a first survey for thepresence in foxes of Echinococcus multilocularis and Toxocara canis was conducted in Brussels from 2001 to 2004. None of 160 foxes were found to be infected with Echinococcus multilocularis and 24 of 134 foxes were found to be infected with Toxocara canis. Considering numbers of examined foxes, the sensitivity and the specificity of tests used for diagnosis, the 95% credibility intervals for the true prevalence of carriage of Echinococcus multilocularis and Toxocara canis were estimated in a Bayesian framework to be 0% to 1.87% (median value of 0%) and 12.7% to 26% (median value of 18.7.%) respectively. For Toxocara canis, a significantly higher risk to be a carrier occurs in cubs and a significantly lower risk in adults. [less ▲]

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See detailKinetics of Microsporum canis adherence using an in vitro model of reconstituted feline epidermis
Baldo, Aline ULg; Tabart, J.; Vermout, S. et al

Conference (2006)

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See detailRecent findings on the pathogenesis of dermatophytoses in dogs and cats
Mignon, Bernard ULg; Vermout, S.; Tabart, J. et al

in Revista Scientia Parasitologica (2006), 7(3/4), 7-15

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See detailTwo Microsporum canis dipeptidyl peptidase genes with possible involvement in fungal virulence
Vermout, S.; Baldo, Aline ULg; Tabart, J. et al

Conference (2006)

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See detailA new in vitro model of Microsporum canis dermatophytosis in reconstituted feline skin
Tabart, J.; Baldo, Aline ULg; Vermout, S. et al

Conference (2006)

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See detailVaccination against dermatophytosis in domestic animals : Past, present and future
Mignon, Bernard ULg; Vermout, S.; Tabart, J. et al

Conference (2006)

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See detailBluetongue in northern Europe.
Thiry, Etienne ULg; Saegerman, Claude ULg; Guyot, Hugues ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2006), 159(10), 327

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See detailUltrastructural morphology of the male and female genital tracts of Psoroptes spp. (Acari : Astigmata : Psoroptidae)
Lekimme, Mireille ULg; Leclercq-Smekens, M.; Devignon, C. et al

in Experimental & Applied Acarology (2005), 36(4), 305-324

The structure of the male and female genital systems of the astigmatid mite Psoroptes ovis (Hering) is described. The male genital system is composed of a paired testis, fused at its proximal part, two ... [more ▼]

The structure of the male and female genital systems of the astigmatid mite Psoroptes ovis (Hering) is described. The male genital system is composed of a paired testis, fused at its proximal part, two vasa deferentia, an ejaculatory duct, into which a single accessory gland opens, and a copulatory organ. The testis is characterized by a peripheric syncytial cell surrounding spermatogonia, spermatocytes, spermatids and spermatozoa which are distributed regularly in the gonad according to the sequence of spermatogenesis. The female genital system consists of a copulatory pore (the bursa copulatrix), a seminal receptacle, paired ovaries and oviducts, a glandular uterus and an ovipositor which leads to the oviporus. Ovaries are composed of somatic cells, germ cells and a central cell, with a multilobular nucleus, connected to oocytes by a stalk. Similarities with other astigmatic mites belonging to Psoroptidia and Acaridia are also discussed. [less ▲]

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See detailClinical, pathological and diagnostic aspects of congenital neosporosis in a series of naturally infected calves
de Meerschman, F.; Focant, Charles ULg; Detry, Jacques et al

in Veterinary Record : Journal of the British Veterinary Association (2005), 157(4), 115-118

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See detailDetection of Echinococcus multilocularis in wild boars in France using PCR techniques against larval form
Boucher, J. M.; Hanosset, R.; Augot, D. et al

in Veterinary Parasitology (2005), 129(3-4), 259-266

Recently, new data have been collected on the distribution and ecology of Echinococcus multilocularis in European countries. Different ungulates species such as pig, goat, sheep, cattle and horse are ... [more ▼]

Recently, new data have been collected on the distribution and ecology of Echinococcus multilocularis in European countries. Different ungulates species such as pig, goat, sheep, cattle and horse are known to host incomplete development of larval E. multilocularis. We report a case of E. multilocularis portage in two wild boars from a high endemic area in France (Department of Jura). Histological examination was performed and the DNA was isolated from hepatic lesions then amplified by using three PCR methods in two distinct institutes. Molecular characterisation of PCR products revealed 99% nucleotide sequence homology with the specific sequence of the U1 sn RNA gene of E. multilocularis, 99 and 99.9% nucleotide sequence homology with the specific sequence of the cytochrome oxydase gene of Echinococcus genus and 99.9% nucleotide sequence homology with a genomic DNA sequence of Echinococcus genus for the first and the second wild boar, respectively. (c) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailDes champignons pour lutter contre les insectes et les acariens
Lekimme, Mireille ULg; Mignon, Bernard ULg; Losson, Bertrand ULg

in Point Vétérinaire (2005), 36(254), 12-13

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See detailA longitudinal epidemiological survey of bovine trypanosomosis and its vectors in the White Volta river basin of Northern Ghana
Mahama, C. I.; Desquesnes, M.; Dia, M. L. et al

in Veterinary Parasitology (2005), 128(3-4), 201-208

A longitudinal epidemiological survey of bovine trypanosomosis and its vectors was carried out in the Volta river basin of Northern Ghana to determine the relationship between cattle management and the ... [more ▼]

A longitudinal epidemiological survey of bovine trypanosomosis and its vectors was carried out in the Volta river basin of Northern Ghana to determine the relationship between cattle management and the incidence of bovine trypanosomosis. Two groups of sentinel cattle under different systems of management, classified as "fully-sedentary" and "partially-sedentary" (depending on the type of management) were followed over a 1-year period starting from March 2003 onwards. Cattle were screened at intervals of 3 months using the huffy coat technique (BCT). Buffy coat specimen from animals that were positive for the BCT and those that were negative, but with a packed cell volume (PCV) of less than 21% were further tested using the polymerase chain reaction (PCR). Plasma from all animals were tested for antibody using the indirect antibody enzyme-linked immunosorbent assay (ELISA). Trypanosomosis challenge was determined in tandem with the epidemiological survey with watering sites of sentinel cattle being the foci of interest. The parasitological prevalence at the start of the survey was higher in the fully-sedentary group (9%) than in the partially-sedentary group (3%). In subsequent visits, however, the parasitological incidence was consistently higher in the partially-sedentary group than in the fully-sedentary group. The mean seroprevalence (ELISA) of both groups increased from 3% in March to 54% in December. Statistical analysis of the serological results using a random effect logistic regression, showed a significant difference in incidence of bovine trypanosomosis between the two groups. There was also a significant effect of time. The influence of cattle herding on host-vector-parasite interface and its consequence on the incidence of trypanosomosis are discussed. (c) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailInfluence of an experimental Trypanosoma congolense infection and plane of nutrition on milk production and some biochemical parameters in West African Dwarf goats
Faye, D.; Fall, A.; Leak, S. et al

in Acta Tropica (2005), 93(3), 247-257

The interactions of trypanosomosis and plane of nutrition on health and productivity of multiparous and primiparous West African Dwarf (WAD) does were studied in a multi-factorial experiment including ... [more ▼]

The interactions of trypanosomosis and plane of nutrition on health and productivity of multiparous and primiparous West African Dwarf (WAD) does were studied in a multi-factorial experiment including diet (supplementation or basal diet) and infection (infected or control). Experimental does were infected with Trypanosoma congolense at the beginning of the second week post-kidding and monitored for 16 weeks after infection. Trypanosome infection significantly reduced packed cell volume (PCV) (control: 30.1 +/- 0.3% versus infected: 22.2 +/- 0.3%; P < 0.0001). Regardless of infection, the drop in PCV from the pre-infection period to the end of the experiment was more severe in animals under restricted diet (interaction diet x period, P < 0.001). Trypanosome parasitaemia tended to be higher in the supplemented group than in the basal diet group (P > 0.05) and multiparous animals had a higher parasitaemia (score: 2.6 +/- 0.1) than primiparous animals (score: 2.2 +/- 0.1) (P < 0.05). Trypanosome infection as well as dietary supplement had a significant effect on lactation length. Milk off-take from trypanosome-infected does was significantly lower than that from the uninfected control group (17.5 +/- 3.21 versus 35.5 +/- 3.21, P < 0.001) and there was a positive effect of plane of nutrition (supplemented: 32.8 +/- 3.21 and basal diet: 20.2 +/- 3.51, P = 0.01). The drop in milk off-take due to trypanosome infection was more severe in the supplemented group (control: 46.7 +/- 4.71 versus infected: 18.9 +/- 4.21) than in the group receiving a basal diet (control: 24.2 +/- 5.01 versus infected: 16.1 +/- 4.71) (interaction infection x diet, P = 0.04) due to the number of does from the supplemented group that were withdrawn from the experiment. The effect of trypanosome infection on doe's live-weight was only noticeable during the first 8 weeks of lactation and there was no significant effect on offspring growth rate unless the mother died. Plasma total protein (TP), albumin and cholesterol concentrations were significantly reduced by the infection but were significantly increased by supplementation. Supplemented does had a higher level of cholesterol and a tendency for a higher parasitaemia. Does of high parity also had a higher cholesterol level than primiparous does and, based on the number of animals that were withdrawn from the experiment, they showed a lower resistance to the infection. (c) 2005 Elsevier B.V. All rights reserved. [less ▲]

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See detailIn vivo m-RNA expression analysis of Microsporum canis secreted subtilisin-like serine proteases in feline dermatophytosis
Mignon, Bernard ULg; Vermout, Sandy; Brouta, F. et al

in Advances in Veterinary Dermatology (2005)

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