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See detailHow to get rid of the two spotted spider mites?
Attia, Sabrine; Grissa, Kaouthar Lebdi; Heuskin, Stéphanie ULiege et al

in Revue des Régions Arides (2014), 35(3), 1739-1748

Two essential oils were tested for their toxicity against eggs and adults of Tetranychus urticae Koch as well as adults of Phytoseiulus persimilis Athias-Henriot, by using a filter paper diffusion ... [more ▼]

Two essential oils were tested for their toxicity against eggs and adults of Tetranychus urticae Koch as well as adults of Phytoseiulus persimilis Athias-Henriot, by using a filter paper diffusion bioassay without allowing direct contact. Responses varied according to oil type and dose, and mite species. The chemical analyses with GC-MS and GC-FID revealed that the two oils differed in their most abundant components. The most abundant components in the Citrus oil were linalyl acetate (41.95%), sabinene (18.60%) and linalool (18.14%) whereas pulegone (41.86%) and menthone (28.33%) were most prevalent in the Mentha oil. Mortality and fecundity were measured with 15 oils concentrations ranged from 0.01 to 8μl/l of air. Tetranychid mortality increased with increasing concentrations with LC50 value of 5.39 and 4.09 μl/l for C. aurantium and M. pulegium, respectively. However few mortality was observed in the case of P. persimilis with LC50 value of 0.46 and 0.26 μl/l for C. aurantium and M. pulegium respectively. For both oils a reduction of fecundity was observed at 0.01 μ/l in the case of T. urticae. The essential oils described herein have potential interest as fumigants for the bio-control of T. urticae. [less ▲]

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See detailWhole-genome sequence of Serratia symbiotica strain CWBI-2.3T, a free-living symbiont of the Black Bean Aphid Aphis fabae
Foray, Vincent; Grigorescu, Alina ULiege; Sabri, Ahmed et al

in Genome Announcements (2014), 2(4),

The gammaproteobacterium Serratia symbiotica is one of the major secondary symbionts found in aphids. Here, we report the draft genome sequence of S. symbiotica strain CWBI-2.3T, previously isolated from ... [more ▼]

The gammaproteobacterium Serratia symbiotica is one of the major secondary symbionts found in aphids. Here, we report the draft genome sequence of S. symbiotica strain CWBI-2.3T, previously isolated from the black bean aphid Aphis fabae. The 3.58-Mb genome sequence might provide new insights to understand the evolution of insect-microbe symbiosis. [less ▲]

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See detailComposition chimique et activité antimicrobienne de l’huile essentielle de Thymus vulgaris du Nord d’Algérie
Sidali, L.; Brada, M.; Fauconnier, Marie-Laure ULiege et al

in PhytoChem & BioSub Journal (2014), 8(3), 156-161

Twenty five constituents were identified by GC MS analysis of essential loils from. Thymus vulgaris. The main compounds identified were carvacrol (55,2%),γ-terpinene (12,6%), p-cymene (9,3%), linalol (3,9 ... [more ▼]

Twenty five constituents were identified by GC MS analysis of essential loils from. Thymus vulgaris. The main compounds identified were carvacrol (55,2%),γ-terpinene (12,6%), p-cymene (9,3%), linalol (3,9%) and α-terpinene (2,8%). The obtained Essential oils exhibited antibacterial and antifungal activities against S.P. Sterptococcus, staphylococcus aureus, E.coli, Klebscila and condidat albicans. [less ▲]

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See detailRole of larval host plant experience and solanaceous plant volatile emissions in Tuta absoluta (Lepidoptera: Gelechiidae) host finding behavior
Caparros Megido, Rudy ULiege; De Backer, Lara; Ettaïb, Refki et al

in Arthropod-Plant Interactions (2014)

The tomato leafminer, Tuta absoluta (Lepi- doptera: Gelechiidae), is considered to be a major pest that damages tomato (Solanum lycopersicum L; Solanaceae) crops in South American, European, and ... [more ▼]

The tomato leafminer, Tuta absoluta (Lepi- doptera: Gelechiidae), is considered to be a major pest that damages tomato (Solanum lycopersicum L; Solanaceae) crops in South American, European, and Mediterranean countries. This insect species is polyphagous (i.e., feeds on many types of food); hence, it could also develop on other cultivated host plants, principally solanaceous plants, such as potato (S. tuberosum L.; Solanaceae) and eggplant (S. melongena L.; Solanaceae). Therefore, we tested the hypothesis that host plant choice by adult T. absoluta is influenced by plant volatile organic compounds and larval host plant experience. One tomato cultivar (cv.) ‘Money- maker’ and three potato cv. ‘Charlotte’ ‘Bintje,’ and ‘Nicola’ were tested. Using a flying tunnel, we observed that females reared on tomato preferred flying toward tomato and, to a lesser extent, potato cv. ‘Charlotte.’ These preferences might be explained by the high release of terpenes by these two plants. When conducting oviposition choice assays, we found no preference between tomato and potato in the number of eggs laid by females that had been previously reared on either host plant. This study indicates that the host finding behavior of T. absoluta is mediated by solanaceous volatiles, while oviposition behavior appears to depend on additional stimuli. These results provide baseline information for use in the development of new control strategies against T. absoluta using semiochemicals and plant breeding. [less ▲]

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See detailThe facultative bacterial symbiont Serratia symbiotica in Acyrtosiphon pisum confer resistance to Aphidius ervi
Attia, Sabrine; Foray, Vincent; Louâpres, Philippe et al

Poster (2014)

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See detailDoes the facultative bacteria Serratia symbiotica influence the foraging strategies of aphid parasitoids?
Attia, Sabrine; Louâpre, Philippe; Foray, Vincent et al

Poster (2014)

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See detailA volatile sex pheromone in the invasive ladybeetle Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae)
Fassotte, Bérénice ULiege; Fischer, Christophe ULiege; Durieux, Delphine ULiege et al

in Communications in Agricultural and Applied Biological Sciences (2014), 79(1), 79-81

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See detailDétermination de la teneur en équol dans les laits commercialisés en Wallonie (Belgique)
Daems, Frédéric ULiege; Jasselette, Christophe; Franckson, Delphine et al

Poster (2013, December 04)

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See detailTechnologie de production de masse d’insectes - INSECTECH
Richard, Gaetan ULiege; Hance, Thierry; Fauconnier, Marie-Laure ULiege et al

Report (2013)

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See detailPhysicochemical properties of lipids extracted from Tenebrio molitor larvae
Danthine, Sabine ULiege; Blecker, Christophe ULiege; Paul, Aman ULiege et al

Poster (2013, December)

Objectives: To determine the physicochemical properties of the oil obtained from Tenebrio molitor Larvae and explore its potential as edible oil. Methodology: Oils obtained from five batches of Tenebrio ... [more ▼]

Objectives: To determine the physicochemical properties of the oil obtained from Tenebrio molitor Larvae and explore its potential as edible oil. Methodology: Oils obtained from five batches of Tenebrio molitor Larvae were investigated. Among the samples, three were produced directly in the lab (3 different productions) and 2 were purchased from a local supplier. In addition to the total lipid content (solvent extraction), both FA (GC) and TAG (HPLC) profiles were determined. Thermal properties by DSC were also estimated. Results and conclusion: The fresh Larvae from the lab contained 52% of total proteins (% dry matter). Their total fat content was around 36% (% dry matter). The commercial samples contained more proteins, but less fat: around 58% of total proteins and 30% of total fat (% dry matter). All the extracted oils contained high amount of unsaturated fatty acids. However, the chemical composition and the thermal properties of the samples varied according to their origin. The level and quality of lipid content offer potential as a substitute of oilseeds. [less ▲]

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See detailLes Ressources Sauvages des Bois de Tapia (Uapaca bojeri) à Madagascar
Barsics, Fanny ULiege; Malaisse, François ULiege; Razafimanantsoa, Tsiresy et al

Poster (2013, October 19)

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See detailFood intake of Tibetan children living in Kashin Beck disease endemic areas in Central Tibet
Rooze, Shancy; de Voghel, Pauline; Mathieu, Françoise et al

Conference (2013, October 14)

Introduction: Kashin-Beck disease (KBD) is a bone and joint pathology. KBD begins during childhood, causes deformities, pain, lack of mobility, and short stature. The pathophysiology of the disease is ... [more ▼]

Introduction: Kashin-Beck disease (KBD) is a bone and joint pathology. KBD begins during childhood, causes deformities, pain, lack of mobility, and short stature. The pathophysiology of the disease is unknown. Nutrition possibly plays a role. Children living in KBD endemic areas in Central Tibet present severe growth retardation, signs of chronic malnutrition and a high prevalence of rickets. A food consumption study was conducted. We have focused our attention on total energy intake, calcium, phosphorus and vitamin D. Material and methods: Prospective food intake measurements have been performed. The investigation concerned children from 9 to 16 years old living in KBD endemic areas. All food and drinks have been measured during several days at the 4 seasons of the year. Anthropometric data were obtained. Results: 30,5% of the children presented with stunting (height for age ratio below -2 z score); 13,8% of the cohort suffered from acute malnutrition (body mass index below -2 z score). Mean total energy intake was 87% of the recommended value. Calcium and vitamin D intakes never covered the needs (44 % and 14 %, respectively). Phosphorus intake was high. Mean Ca / P ratio was 0.54. Conclusion: Calcium and vitamin D deficiency may play a role in the etiology of bone and joint diseases in this population. To improve the nutritional status of the children, a large program of calcium and vitamin D supplementation among 0 - 5 years old children living in KDB endemic areas was started. Besides, chemical analyses of locally grown foods are performed, in order to have a more precise evaluation of nutrient intake. In the future, the effect of increased energy intakes will be evaluated. [less ▲]

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See detailFirst Evidence of a Volatile Sex Pheromone in Harmonia axyridis (Pallas)
Fassotte, Bérénice ULiege; Fischer, Christophe ULiege; Durieux, Delphine ULiege et al

Poster (2013, October)

Until now, no volatile sex pheromone has been highlighted in Coccinellidae but various studies have suggested the existence of such molecules. In the present work, we have sampled volatile organic ... [more ▼]

Until now, no volatile sex pheromone has been highlighted in Coccinellidae but various studies have suggested the existence of such molecules. In the present work, we have sampled volatile organic compounds released in the headspace of virgin females in the multicolored Asian ladybeetle, Harmonia axyridis (Pallas), that were either allowed or not to feed on pea aphids (Acyrthosiphon pisum Harris). When fed on aphids, virgin females showed a stereotypical “calling behavior”, commonly associated with the emission of a sex pheromone in several Coleoptera species. Behavioral assays conducted with calling females in a four-arm olfactometer demonstrated that the blend of released volatile compounds was attractive at a distance for males, but not for other females. The headspace of virgin females that were not previously fed with aphids was not attractive for either sex. GC-MS analyses revealed the presence of five compounds in the volatile blend: (–)-β-caryophyllene, β-elemene, methyl-eugenol, α-humulene and α-bulnesene. Subsequently, we have collected and quantified the constituents from the blend over a period of 9 days after exposure to aphids. All five compounds were produced exclusively after feeding virgin females with aphids, and their quantity significantly increased during the whole period of collection. (–)-β-caryophyllene was found to be the major constituent, representing between 80 and 86 % of the total blend. All these results could promote the development of more specific and efficient management methods to manipulate the movements of this invasive ladybeetle and to reduce its negative impacts on biodiversity. [less ▲]

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See detailSerratia symbiotica from Aphis fabae: a step forward in understanding the genomic evolution of a lifestyle transition from free-living to aphid-dependent
Grigorescu, Alina ULiege; Foray, Vincent; Sabri, Ahmed et al

Scientific conference (2013, October)

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See detailFirst evidence of a volatile sex pheromone in the invasive Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae)
Fassotte, Bérénice ULiege; Fischer, Christophe ULiege; Durieux, Delphine ULiege et al

Conference (2013, September 10)

Until now, volatile sex pheromone have not been identified in the Coccinellidae, although various studies have suggested the existence of such molecules. In this work, we have sampled volatile organic ... [more ▼]

Until now, volatile sex pheromone have not been identified in the Coccinellidae, although various studies have suggested the existence of such molecules. In this work, we have sampled volatile organic compounds released in the headspace of virgin females of the multicolored Asian ladybeetle, Harmonia axyridis (Pallas), that were either allowed or not to feed on pea aphids (Acyrthosiphon pisum Harris). When fed on aphids, virgin females showed a stereotypical “calling behavior”, commonly associated with the emission of a sex pheromone in several Coleoptera species. Behavioral assays conducted with calling females in a four-arm olfactometer demonstrated that the blend of released volatile compounds was attractive for males, but not for other females. The headspace of virgin females that were not previously fed with aphids was not attractive for either sex. GC-MS analyses revealed the presence of five compounds in the volatile blend: (–)-β-caryophyllene, β elemene, methyl-eugenol, α-humulene and α-bulnesene. Subsequently, we have collected and quantified the constituents from the blend over a period of 9 days after exposure to aphids. All five compounds were produced exclusively after feeding virgin females with aphids, and their quantity significantly increased during the whole period of collection. (–)-β-caryophyllene was found to be the major constituent, representing between 80 and 89 % of the total blend. These results could promote the development of more specific and efficient management methods to manipulate the movements of this invasive ladybeetle and to reduce its negative impacts on biodiversity. [less ▲]

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See detailEmission of sulfur-containing volatiles from Arabidopsis thaliana (L.) Heynh Col-0 related to diamondback moth (Plutella xylostella (L.)) infestation
Hien, Truong Thi Dieu ULiege; Delaplace, Pierre ULiege; Francis, Frédéric ULiege et al

Poster (2013, June 10)

Herbivore-infested plants often release a variety of volatile organic compounds (VOCs). Here, we studied the effects of feeding Plutella xylostella (L.) (0, 3, 9, 20 pest larvae within 0-4 h and 4-8 h ... [more ▼]

Herbivore-infested plants often release a variety of volatile organic compounds (VOCs). Here, we studied the effects of feeding Plutella xylostella (L.) (0, 3, 9, 20 pest larvae within 0-4 h and 4-8 h infestation, respectively) on the emission of sulfur-containing VOCs in Arabidopsis thaliana (L.) Heynh Col-0 (A.t. Col-0) by headspace solid-phase micro-extraction coupled to gas chromatography - mass spectrometry (HS-SPME-GC/MS). The analytical results showed that the relative emission of sulfur-containing metabolites increased significantly in Arabidopsis plants subjected to P. xylostella infestation according to the density and residence duration of pest larvae on shoot organs. The main compound from infested plants was dimethyl disulfide. We suggest that the correlations between the stress level (density and time infestation) and the sulfides observed in this study provide a means to understand the changes of VOCs profile of plant under chewer infestation. [less ▲]

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