References of "Leprince, Pierre"
     in
Bookmark and Share    
Full Text
See detailReproductive impact and proteomic analysis of androgenic and anti-androgenic disruptors on the hermaphroditic freshwater gastropod Lymnaea stagnalis
Giusti, Arnaud ULg; Ducrot, Virginie; Joaquim-Justo, Célia ULg et al

Poster (2011, November)

In this study, e ects of two androgens (tributyltin and testosterone), one antiandrogen (cyproterone acetate) and one estrogen (chlordecone) on growth and reproduction were investigated in the ... [more ▼]

In this study, e ects of two androgens (tributyltin and testosterone), one antiandrogen (cyproterone acetate) and one estrogen (chlordecone) on growth and reproduction were investigated in the hermaphrodite gastropod Lymnaea stagnalis. In this study, exposure to a range of concentrations (ng/l to μg/l) of each chemical was performed during 21 days. e number of clutches and the number of eggs per clutch were monitored. A decrease in clutches laid per snail was observed after exposure to tributyltin (540, 1180 and 2600 ng/l) and chlordecone (10, 22, 50 and 110 μg/l). A signi cant decrease in egg laid per snail was observed after exposure to tributyltin (540, 1180 and 2600 ng/l) and chlordecone (50 and 110 μg/l). An increase of egg abnormalities ratio in exposed snails (atrophied albumen, polyembryonicity,...) was observed in L. stagnalis following exposure to testosterone (2, 22, 50 and 110 ng/l), cyproterone acetate (4,5 and 50 μg/l), tributyltin (110 and 244 ng/l) and chlordecone (4,5 and 10 μg/l). Investigation of alteration in protein expression in exposed snails was performed using proteomic analysis such as 2D-DIGE. Mass spectrometry identi cation was performed on proteins with altered expression. We could establish correlation between reproductive endpoints and changes in proteins involved in egg formation and in egg laying were underlined. Egg yolk ferritin, the main protein of egg yolk, was shown to be reduced signi cantly in relationship with a decrease of egg yolk quality after exposure to tributyltin 540 ng/l and cyproterone acetate 4,5 μg/l. Ovipostatin, a protein proved to reduce egg masses, was signi cantly over expressed in snails exposed to 50 μg/l of chlordecone and were in relationship with a reduction of clutches laid by individuals. Further western blot analysis on those proteins involved in the reproduction are underway. ese analysis will enable us to con rm and re ne with more speci city the 2D-DIGE results for the selected proteins. e results of this study can help to establish new biomarkers of exposure of endocrine disruptors in freshwater environment and can provide new insight on mode of action of endocrine disruptors in L. stagnalis. [less ▲]

Detailed reference viewed: 42 (5 ULg)
Full Text
Peer Reviewed
See detailWnt1 and BMP2: two factors recruiting multipotent neural crest progenitors isolated from adult bone marrow
Glejzer, Aneta ULg; Laudet, Emerence ULg; Leprince, Pierre ULg et al

in Cellular and Molecular Life Sciences : CMLS (2011), 68/12

Recent studies have shown that neural crestderived progenitor cells can be found in diverse mammalian tissues including tissues that were not previously shown to contain neural crest derivatives, such as ... [more ▼]

Recent studies have shown that neural crestderived progenitor cells can be found in diverse mammalian tissues including tissues that were not previously shown to contain neural crest derivatives, such as bone marrow. The identification of those ‘‘new’’ neural crest-derived progenitor cells opens new strategies for developing autologous cell replacement therapies in regenerative medicine. However, their potential use is still a challenge as only few neural crest-derived progenitor cells were found in those new accessible locations. In this study, we developed a protocol, based on wnt1 and BMP2 effects, to enrich neural crest-derived cells from adult bone marrow. Those two factors are known to maintain and stimulate the proliferation of embryonic neural crest stem cells, however, their effects have never been characterized on neural crest cells isolated from adult tissues. Using multiple strategies from microarray to 2D-DIGE proteomic analyses, we characterized those recruited neural crest-derived cells, defining their identity and their differentiating abilities. [less ▲]

Detailed reference viewed: 104 (34 ULg)
Full Text
Peer Reviewed
See detailUpdate in the methodology of the chronic stress paradigm: internal control matters
Strekalova, Tatyana; Couch; Kholod, Natalia et al

in Behavioral and Brain Functions : BBF (2011), 7

To date, the reliability of induction of a depressive-like state using chronic stress models is confronted by many methodological limitations. We believe that the modifications to the stress paradigm in ... [more ▼]

To date, the reliability of induction of a depressive-like state using chronic stress models is confronted by many methodological limitations. We believe that the modifications to the stress paradigm in mice proposed herein allow some of these limitations to be overcome. Here, we discuss a variant of the standard stress paradigm, which results in anhedonia. This anhedonic state was defined by a decrease in sucrose preference that was not exhibited by all animals. As such, we propose the use of non-anhedonic, stressed mice as an internal control in experimental mouse models of depression. The application of an internal control for the effects of stress, along with optimized behavioural testing, can enable the analysis of biological correlates of stressinduced anhedonia versus the consequences of stress alone in a chronic-stress depression model. This is illustrated, for instance, by distinct physiological and molecular profiles in anhedonic and non-anhedonic groups subjected to stress. These results argue for the use of a subgroup of individuals who are negative for the induction of a depressive phenotype during experimental paradigms of depression as an internal control, for more refined modeling of this disorder in animals [less ▲]

Detailed reference viewed: 31 (6 ULg)
Full Text
Peer Reviewed
See detailPotential Therapeutic Target Discovery by 2D-DIGE Proteomic Analysis in Mouse Models of Asthma
QUESADA CALVO, Florence ULg; Fillet, Marianne ULg; Renaut, Jenny et al

in Journal of Proteome Research (2011), 10(9), 4291-4301

As asthma physiopathology is complex and not fully understood to date; it is expected that new key mediators are still to be unveiled in this disease. The main objective of this study was to discover ... [more ▼]

As asthma physiopathology is complex and not fully understood to date; it is expected that new key mediators are still to be unveiled in this disease. The main objective of this study was to discover potential new target proteins with a molecular weight >20 kDa by using two-dimensional differential in-gel electrophoresis (2D-DIGE) on lung parenchyma extracts from control or allergen-exposed mice (ovalbumin). Two different mouse models leading to the development of acute airway inflammation (5 days allergen exposure) and airway remodeling (10 weeks allergen exposure) were used. This experimental setting allowed the discrimination of 33 protein spots in the acute inflammation model and 31 spots in the remodeling model displaying a differential expression. Several proteins were then identified by MALDI-TOF/TOF MS. Among those differentially expressed proteins, PDIA6, GRP78, Annexin A6, hnRPA3, and Enolase display an increased expression in lung parenchyma from mice exposed to allergen for 5 days. Conversely, Apolipoprotein A1 was shown to be decreased after allergen exposure in the same model. Analysis on lung parenchyma of mice exposed to allergens for 10 weeks showed decreased calreticulin levels. Changes in the levels of those different mediators were confirmed by Western blot and immunohistochemical analysis. Interestingly, alveolar macrophages isolated from lungs in the acute inflammation model displayed enhanced levels of GRP78. Moreover, intratracheal instillation of anti-GRP78 siRNA in allergen-exposed animals led to a decrease in eosinophilic inflammation and bronchial hyperresponsiveness. This study unveils new mediators of potential importance that are up- and down-regulated in asthma. Among up-regulated mediators, GRP-78 appears as a potential new therapeutic target worthy of further investigations. [less ▲]

Detailed reference viewed: 58 (18 ULg)
Full Text
Peer Reviewed
See detailDynamics of the Dictyostelium discoideum mitochondrial proteome during vegetative growth, starvation and early stages of development
Czarna, Malgorzata; Mathy, Grégory ULg; Mac Cord, Allan ULg et al

in Proteomics (2010), 9

In this study a quantitative comparative proteomics approach has been used to analyze the D. discoideum mitochondrial proteome variations during vegetative growth, starvation and the early stages of ... [more ▼]

In this study a quantitative comparative proteomics approach has been used to analyze the D. discoideum mitochondrial proteome variations during vegetative growth, starvation and the early stages of development. Application of 2D-DIGE technology allowed the detection of around 2000 protein spots on each two-dimensional gel with 180 proteins exhibiting significant changes in their expression level. In total, 96 proteins (51 unique and 45 redundant) were unambiguously identified. We show that the D. discoideum mitochondrial proteome adaptations mainly affect energy metabolism enzymes (the Krebs cycle, anaplerotic pathways, the oxidative phosphorylation system and energy dissipation), proteins involved in developmental and signalling processes as well as in protein biosynthesis and fate. The most striking observations were the opposite regulation of expression of citrate synthase and aconitase and the very large variation in the expression of the alternative oxidase (AOX) that highlighted the importance of citrate and AOX in the physiology of the development of D. discoideum. Mitochondrial energy states measured in vivo with MitoTracker Orange CMTMRos showed an increase in mitochondrial membrane polarisation during D. discoideum starvation and starvation-induced development. [less ▲]

Detailed reference viewed: 154 (39 ULg)
Peer Reviewed
See detailProteomic study of cold-repressed proteins in the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125
Piette, Florence; D'Amico, Salvino; Leprince, Pierre ULg et al

Poster (2010)

Detailed reference viewed: 11 (1 ULg)
Full Text
Peer Reviewed
See detailProteomic and functional characterization of a Chlamydomonas reinhardtii mutant lacking the mitochondrial alternative oxidase 1
Mathy, Grégory ULg; Cardol, Pierre ULg; Dinant, Monique et al

in Journal of Proteome Research (2010), 9

In the present work we have isolated by RNA interference and characterized at the functional and the proteomic levels a Chlamydomonas reinhardtii strain devoid of the mitochondrial alternative oxidase ... [more ▼]

In the present work we have isolated by RNA interference and characterized at the functional and the proteomic levels a Chlamydomonas reinhardtii strain devoid of the mitochondrial alternative oxidase (AOX). The AOX-deficient strain displays a doubling of the cell volume and biomass without any alteration of the generation time, a significantly higher ROS production, no change in total respiration rate, and a slight decrease of the photosynthesis efficiency. In order to identify the molecular adaptation underlying these phenotypical effects, we carried out a comparative proteomic study at the level of the mitochondrial and cellular soluble proteomes. Our results indicate a strong up-regulation of the ROS scavenging systems and important modifications of proteins involved in the primary metabolism, namely an increase of enzymes involved in anabolic pathways and a concomitant general down-regulation of enzymes of the main catabolic pathways. [less ▲]

Detailed reference viewed: 200 (97 ULg)
Full Text
Peer Reviewed
See detailTritrophic interactions among Macrosiphum euphorbiae aphids, their host plants and endosymbionts: investigation by a proteomic approach.
Francis, Frédéric ULg; Guillonneau, F.; Leprince, Pierre ULg et al

in Journal of Insect Physiology (2010), 56(6), 575-85

The Mi-1.2 gene in tomato confers resistance against certain clones of the potato aphid (Macrosiphum euphorbiae). This study used 2D-DIGE coupled with protein identification by MALDI-TOF-MS to compare the ... [more ▼]

The Mi-1.2 gene in tomato confers resistance against certain clones of the potato aphid (Macrosiphum euphorbiae). This study used 2D-DIGE coupled with protein identification by MALDI-TOF-MS to compare the proteome patterns of avirulent and semivirulent potato aphids and their bacterial endosymbionts on resistant (Mi-1.2+) and susceptible (Mi-1.2-) tomato lines. Avirulent aphids had low survival on resistant plants, whereas the semivirulent clone could colonize these plants. Eighty-two protein spots showed significant quantitative differences among the four treatment groups, and of these, 48 could be assigned putative identities. Numerous structural proteins and enzymes associated with primary metabolism were more abundant in the semivirulent than in the avirulent aphid clone. Several proteins were also up-regulated in semivirulent aphids when they were transferred from susceptible to resistant plants. Nearly 25% of the differentially regulated proteins originated from aphid endosymbionts and not the aphid itself. Six were assigned to the primary endosymbiont Buchnera aphidicola, and 5 appeared to be derived from a Rickettsia-like secondary symbiont. These results indicate that symbiont expression patterns differ between aphid clones with differing levels of virulence, and are influenced by the aphids' host plant. Potentially, symbionts may contribute to differential adaptation of aphids to host plant resistance. [less ▲]

Detailed reference viewed: 73 (19 ULg)
See detailProteomic comparison of High and Low responders to eccentric exercise
Hody, Stéphanie ULg; Croisier, Jean-Louis ULg; Leprince, Pierre ULg et al

in Abstract book du Colloque de la Société Belge des Neurosciences (2009, May)

Detailed reference viewed: 29 (10 ULg)
Full Text
Peer Reviewed
See detailCourbatures après exercice excentrique : High et Low responders ?
Hody, Stéphanie ULg; Rogister, Bernard ULg; Croisier, Jean-Louis ULg et al

in Croisier, Jean-Louis; Codine, Philippe (Eds.) Exercice musculaire excentrique (2009)

It is well known that novel and/or unusual exercise consisting of lengthening muscle contractions (eccentric exercise) results in muscle damage. Muscle damage can be assessed via measures of indirect ... [more ▼]

It is well known that novel and/or unusual exercise consisting of lengthening muscle contractions (eccentric exercise) results in muscle damage. Muscle damage can be assessed via measures of indirect markers such as isometric strength, range of motion, blood levels of muscle-specific proteins and muscle soreness. It has been shown that changes in these markers present a large inter-subject variability. Especially, the seric CK response to exercise is largely documented and some authors introduced the notion of High and Low responders to define subjects with exaggerated and light or moderate response to eccentric protocol, respectively. The reasons for the various susceptibility to eccentric exercise are not understood but it seems to be influenced by several factors including genetical, morphological, physiological or training elements. Further studies are necessary to elucidate the underlying mechanisms of the large inter-subject variability in responses to eccentric exercise and in this context, a proteomic approach could be interesting to highlight protein expression differences between High and Low responders. [less ▲]

Detailed reference viewed: 149 (37 ULg)
Full Text
Peer Reviewed
See detailDOMS : traiter ou prévenir ?
Hody, Stéphanie ULg; Rogister, Bernard ULg; Leprince, Pierre ULg et al

in Croisier, Jean-Louis; Codine, Philippe (Eds.) Exercice musculaire excentrique (2009)

The evidence of benefits from eccentric contraction provides compelling rationale for their inclusion in force training and in the reeducation of various locomotor pathologies (i.e., tendinopathies or ... [more ▼]

The evidence of benefits from eccentric contraction provides compelling rationale for their inclusion in force training and in the reeducation of various locomotor pathologies (i.e., tendinopathies or muscular imbalance). However, intense or unusual eccentric exercise frequently leads to muscle damage associated with prolonged loss in muscle strength and range of motion, a dramatic increase in muscle proteins circulating in blood and the development of muscle soreness. These negative functional consequences, referred to as Delayed Onset Muscle Soreness (DOMS), often disturb the progress of training or reeducation programmes. In an attempt to attenuate signs and symptoms of DOMS, several approaches have been used prophylactically and/or therapeutically. This article focuses on some of the most commonly used modalities, including the nutritionnal and pharmacological strategies, clinical therapies and exercise. Despite a large number of studies, there is little evidence indicating any benefit at the perfomance level of these various approaches. Conversely, there is unequivocal evidence that a bout of eccentric exercise is followed by protection against skeletal muscle damage associated with a subsequent bout of potentially damaging exercise for several weeks to several months. To date, specific submaximal training appears the most efficient prophylactic measure and is recommended to alleviate the prejudiciable consequences associated with DOMS in the athletic or therapeutic programs. Despite the volume of data concerning this protective effect, often called the “repeated bout effect”, the underlying mechanisms of such an adaptation are not fully understood and a better knowledge of these adaptative mechanisms could provide better guidelines for prevention or treatment practice. [less ▲]

Detailed reference viewed: 153 (38 ULg)
Full Text
Peer Reviewed
See detailThe proapoptotic C16-ceramide-dependent pathway requires the death-promoting factor Btf in colon adenocarcinoma cells.
Renert, Anne-Francoise; Leprince, Pierre ULg; Dieu, Marc et al

in Journal of Proteome Research (2009), 8(10), 4810-22

Ceramides are central molecules in sphingolipid metabolism. They are involved in the regulation of cancer-cell growth, differentiation, senescence and apoptosis. To better understand how these secondary ... [more ▼]

Ceramides are central molecules in sphingolipid metabolism. They are involved in the regulation of cancer-cell growth, differentiation, senescence and apoptosis. To better understand how these secondary messengers induce their biological effects, adenocarcinoma cells (HCT116) were treated with exogenous long-chain ceramides (C16-ceramide) in order to mimic endogenous sphingolipids. This treatment induced a decrease of cell viability partly due to apoptosis as shown by PARP cleavage and a decrease of pro-caspase 3. Two-dimensional differential in-gel electrophoresis (2D-DIGE) revealed the differential expression of 51 proteins in response to C16-ceramide. These proteins are notably involved in cell proliferation, apoptosis, protein transport and transcriptional regulation. Among them, the cell death-promoting factor Btf was found to be implicated in the apoptotic signal triggered by ceramide. In adenocarcinoma cells, Btf regulates apoptosis related proteins such as Mdm2, p53, BAX and pBcl-2 and thus plays an important role in the ceramide mediated cell death. These findings bring new insight into the proapoptotic ceramide-dependent signaling pathway. [less ▲]

Detailed reference viewed: 78 (23 ULg)
See detailProtein synthesis and folding is a rate limiting step for bacterial growth at low temperatures.
Piette, Florence; D'Amico, Salvino; Leprince, Pierre ULg et al

Poster (2008)

Detailed reference viewed: 9 (1 ULg)
Peer Reviewed
See detailProtein synthesis and folding is a rate limiting step for bacterial growth at low temperatures.
Piette, Florence; D'Amico, Salvino; Leprince, Pierre ULg et al

Poster (2008)

Detailed reference viewed: 5 (1 ULg)
Peer Reviewed
See detailThe cold-adapted trigger factor from the Antarctic bacterium Pseudoalteromonas haloplanktis.
D'Amico, Salvino; Piette, Florence; Leprince, Pierre ULg et al

Poster (2008)

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailInterest of a comparative proteomic approach to unravel the aetiology of DOMS in humans
Hody, Stéphanie ULg; Croisier, Jean-Louis ULg; Wang, François-Charles ULg et al

in Acta Physiologica Scandinavica. Supplementum (2008), 194(suppl 666), -09

Detailed reference viewed: 42 (16 ULg)