References of "Lepoivre, Philippe"
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See detailEvaluation of two protein extraction protocols for Pichia anomala proteome analysis.
Bajji, Mohammed; Delaplace, Pierre ULg; Mauro, Sergio et al

Poster (2009, May 19)

Pichia anomala (strain Kh6) was isolated from the surface of apple fruits and selected for its high and reliable biocontrol activity against Botrytis cinerea and Penicillium expansum. Its main modes of ... [more ▼]

Pichia anomala (strain Kh6) was isolated from the surface of apple fruits and selected for its high and reliable biocontrol activity against Botrytis cinerea and Penicillium expansum. Its main modes of action have until now been studied using essentially microbiological and molecular approaches. The study continues now using the proteomic approach and considering the in situ P. anomala/B. cinerea/apple interaction. Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) is one of the most powerful tools used for proteomic analysis. It combines two sequential separation steps, the first dimension via isoelectric focusing (IEF) and the second one by SDS PAGE. Although recent advances in 2-D PAGE, the extraction of the whole proteome and the removal of interfering contaminants still limit its application. Sample preparation constitutes indeed a critical influential step for IEF which in turn affects 2-D gel quality. The objective of the present work was thus to develop an effective protein extraction protocol designed for 2-D PAGE analysis of the proteome of P. anomala strain Kh6. As a starting point, two contrasting protein extraction protocols were chosen to be evaluated in terms of protein yield and one-dimensional (1-D) SDS PAGE and 2-D PAGE gel patterns. The first protocol uses a urea/thiourea-based lysis buffer whereas the second protocol utilizes a hot SDS-based lysis buffer with an additional precipitation step. The comparison model used consisted of apples treated with strain Kh6 alone (K) and apples first treated with Kh6 and then inoculated with B. cinerea conidia (KB). Growth kinetics of strain Kh6 on wounded apples was determined and found to be not affected by the presence of B. cinerea conidia. Proteins were extracted from yeast pellets collected at both the exponential and stationary phases of strain Kh6 growth on apples. The evaluation of both extraction protocols indicates that more proteins were extracted with the SDS protocol and, according to 1-D assays, higher molecular weight proteins were obtained with the ‘urea/thiourea’ protocol and, regardless of the protocol used, more bands were obtained during the exponential phase. 2-D assays are currently underway and the corresponding results will be presented. [less ▲]

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See detailA Simple And Rapid Protocol Of Crude Dna Extraction From Apple Trees For Pcr And Real-Time Pcr Detection Of 'Candidatus Phytoplasma Mali'
Aldaghi, M.; Massart, Sébastien ULg; Dutrecq, O. et al

in Journal of Virological Methods (2009), 156(1-2), 96-101

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See detailevaluation of two protein extractions protocols for pichia anomala proteome analysis
Bajji, M.; Delaplace, Pierre ULg; Mauro, S. et al

Poster (2009)

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See detailThe post-harvest quality of bananas is determined by pre-harvest factors
de Lapeyre de Bellaire, Luc; Chillet, Marc; Lassois, Ludivine ULg et al

Poster (2009)

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See detailPreliminary evaluation of antimicrobial activity of some chemicals on in vitro apple shoots infected by 'Candidatus Phytoplasma mali'.
Aldaghi, M.; Massart, Sébastien ULg; Druart, Ph et al

in Communications in Agricultural and Applied Biological Sciences (2008), 73(2),

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See detailApplication Of A Simplified Molecular Protocol To Reveal Mixed Infections With Begomoviruses In Cassava
Busogoro, Jp.; Masquellier, L.; Kummert, J. et al

in Journal of Phytopathology (2008), 156(7-8), 452-457

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See detailImmunogold Silver Staining Associated With Epi-Fluorescence For Cucumber Mosaic Virus Localisation On Semi-Thin Sections Of Banana Tissues
Helliot, B.; Panis, B.; Busogoro, Jp. et al

in European Journal of Histochemistry (2007), 51(2),

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See detailAnalysis of the mechanisms of action of Mycosphaerella fijiensis toxins during the development of Black Leaf Streak disease.
Busogoro, J. P.; Etame, J. J.; Lognay, Georges ULg et al

in Mohan Jain, S.; Swennen, Rony (Eds.) Banana improvement: cellular, molecular biology, and induced mutations.Proceedings of a meeting held in Leuven, Belgium, 24-28 September 2001 (2004)

The goal of our programme is to understand the role of M. fijiensis toxins in Black Leaf Streak disease to facilitate early selection of banana genotypes resistant to the disease. Toxic effects induced by ... [more ▼]

The goal of our programme is to understand the role of M. fijiensis toxins in Black Leaf Streak disease to facilitate early selection of banana genotypes resistant to the disease. Toxic effects induced by crude extracts, as well as by purified juglone released by the pathogen in culture filtrates, were analysed on two reference cultivars of banana. Injection of the pathogen metabolites into banana leaves showed a light-dependent toxicity revealed by the development of necrosis and a rapid decrease of the relative vitality index determined from chlorophyll fluorescence measurements. We demonstrated that juglone, a purified metabolite of M. fijiensis, has a direct inhibitory effect on the electron transfer properties of purified banana chloroplasts. Regardless of the bioassay performed, the susceptibility of the two reference cultivars to this metabolite was correlated with their respective sensitivity to the pathogen infection. On the other hand, some preliminary data showed that juglone initiates oxidation of ascorbic acid, the most abundant antioxidant system in plants. On this base, we hypothesized that oxidative damage could be involved in the action of M. fijiensis toxins. [less ▲]

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See detailStatistically reliable sampling becomes realistic and economically workable with molecular tests
Lepoivre, Philippe ULg; Kummert, J.; Dutrecq, O. et al

Conference (2004)

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See detailRecombinant antibody an its application in plant pathogen diagnosis
Tassus, X.; Duterme, O.; Roussel, S. et al

Poster (2004)

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See detailDevelopment of RT-PCR assays using fluorogenic 3' minor groove binder DNA probes for detection of fruit trees viruses
Roussel, S.; Kummert, J.; Salmon, M. et al

Conference (2004)

Detailed reference viewed: 11 (1 ULg)