IRF3 is essential for house dust mite-induced airway allergy in mice

in Proceedings GIGA-Day 2011 (2011, May)

IRF3-dependent activation of inflammatory dendritic cells by extracellular host DNA mediates the adjuvant activity of alum on TH2 responses

Conference (2011)

Release and innate immune detection of host cell DNA mediate the adjuvant activity of aluminum salts

Conference (2011)

Le microdamier d’expression : un outil performant dans la compréhension de la réponse

in Annales de Médecine Vétérinaire (2011), 154

Malgré de multiples études, les interrelations entre l’exercice, le stress et la réponse immune sont peu définies. Or, la compréhension de ces interrelations pourrait jouer un rôle très important dans ... [more ▼]

Malgré de multiples études, les interrelations entre l’exercice, le stress et la réponse immune sont peu définies. Or, la compréhension de ces interrelations pourrait jouer un rôle très important dans l’amélioration de la santé et des résultats sportifs des athlètes. En effet, l’exercice peut être reconnu comme un stress. Il induit des modifications de l’équilibre homéostatique qui peuvent à leur tour altérer la réponse immunitaire de l’hôte et donc sa susceptibilité aux maladies. L’adrénaline est la molécule essentielle de tout processus de stress. La technologie des microdamiers, outil majeur d’investigation transcriptomique, permet l’étude de l’expression génique de l’ensemble du génome. Son utilisation devrait donc permettre de mieux caractériser et définir les interrelations entre l’exercice, le stress et la réponse immune. Cette revue recense les interrelations connues entre la réponse immune à un stress adrénergique d’une part et la réponse immune à l’exercice d’autre part. Elle considère en outre la contribution potentielle des microdamiers à une meilleure compréhension des effets d’un stress, et plus particulièrement celui lié à l’exercice, sur l’immunité. [less ▲]

Investigation of the innate immunity in the lower respiratory tract in exercising horses

in Proceedings of the 1st Scientific Meeting of the Faculty of Veterinary Medicine (2011)

Results of a haplotype-based GWAS for recurrent laryngeal neuropathy in the horse.

in Mammalian Genome : Official Journal of the International Mammalian Genome Society (2011)

Recurrent laryngeal neuropathy (RLN) is a major upper-airway disease of horses that causes abnormal respiratory noise during exercise and can impair performance. Etiopathogenesis remains unclear but ... [more ▼]

Recurrent laryngeal neuropathy (RLN) is a major upper-airway disease of horses that causes abnormal respiratory noise during exercise and can impair performance. Etiopathogenesis remains unclear but genetic factors have been suspected for many decades. The objective of this study was to identify risk loci associated with RLN. To that end we genotyped 234 cases (196 Warmbloods, 20 Trotters, 14 Thoroughbreds, and 4 Draft horses), 228 breed-matched controls, and 69 parents with the Illumina Equine SNP50 BeadChip. Using these data, we quantified population structure and performed single-marker and haplotype-based association studies, as well as family-based linkage analyses. We accounted for population stratification by modeling a random polygenic background effect with covariance structure estimated from genome-wide SNP data. Using the haplotype-based approach, we identified two genome-wide suggestive loci in Warmbloods, respectively on chromosomes 21 (p = 1.62 x 10(-6)) and 31 (p = 1.69 x 10(-5)). The two signals were driven by the enrichment of a "protective" haplotype in controls compared to cases. [less ▲]

IRF3-dependent activation of inflammatory dendritic cells by extracellular host DNA mediates the adjuvant activity of alum on TH2 responses

Poster (2011)

Genomic expression evaluation: introduction and perspectives

Conference (2010, July)

In a way, disease could be considered as the result of gene expression deregulation. Knowing what genes are expressed in a particular cell or tissue and how they are regulated allow to better understand ... [more ▼]

In a way, disease could be considered as the result of gene expression deregulation. Knowing what genes are expressed in a particular cell or tissue and how they are regulated allow to better understand disease mechanisms. Transcriptomic is the study of the expression of the entire genome. This technical challenge has been rendered possible as different species’ genomes began to be sequenced. Several techniques have rendered efficient this process of measuring simultaneously the expression level of a large number of genes. To date, micro-array technology is probably the best known. However, with the development of more affordable techniques, sequencing/ resequencing could compete with microarrays in the future. All together, these techniques open new ways for early diagnosis and prognosis for the practioner ‘in the field’. [less ▲]

Interferon Response Factor 3 is essential for house dust mite-induced airway allergy

in Journal of Allergy and Clinical Immunology (The) (2010), 126(4), 836-844

IRF3, mainly known as a central orchestrator of antiviral responses, is required for proallergic functions of dendritic cells in response to aeroallergens. Thus, this study demonstratively identifies a ... [more ▼]

IRF3, mainly known as a central orchestrator of antiviral responses, is required for proallergic functions of dendritic cells in response to aeroallergens. Thus, this study demonstratively identifies a new pathway potentially implicated in the etiology of airway allergy [less ▲]

Pulmonary function, airway cytology and bronchoalveolar lavage fluid drug concentration after aerosol administration of cefquinome to horses

in Equine Veterinary Education (2010), 22(9), 473-479

The administration of antibiotics by aerosol to horses suffering from respiratory infections may partially circumvent the limitations of antimicrobial therapy, e.g. large injection volumes, low ... [more ▼]

The administration of antibiotics by aerosol to horses suffering from respiratory infections may partially circumvent the limitations of antimicrobial therapy, e.g. large injection volumes, low bioavailability and risk of diarrhea. Only injectable formulations are available currently and usually contain other substances that could irritate the mucosa and induce coughing and bronchospasm. In addition, the quality of the aerosol, particularly in terms of the delivery of antibiotics to the deep parts of the lung, is unknown. Although used under field conditions, cefquinome delivered by aerosol has never been studied in horses. This study examined the safety of cefquinome injectable solution, administered by aerosol at a dose of 225 mg/inhalation to 7 healthy horses, by assessing (1) pulmonary function before and 15 min after a single inhalation, at the first day (Day 1) and the fifth day (Day 5) of a 5 day period treatment; and (2) the inflammatory status of the lung, i.e. percentage neutrophils and myeloperoxidase concentration, based on bronchoalveolar lavage (BAL) at D1 and D5. In addition, cefquinome concentrations were measured in bronchoalveolar lavage fluid after aerosol, intravenous (i.v.) and intramuscular (i.m.) administrations. A single aerosol of cefquinome injectable solution did not induce any immediate nor delayed pulmonary side effects in healthy horses and produced cefquinome concentrations in bronchoalveolar lavage (BAL) within 30 min that were higher than the minimal inhibitory concentration of the main equine respiratory pathogens. These results should stimulate further studies, especially in horses suffering from bronchial hyper-reactivity. Aerosol delivery of antibiotics may well have a role in equine therapeutics. [less ▲]