Showing results 21 to 40 of 56
  A LC-chip nanoelectrospray MS/MS method for hepcidin quantitation in human plasmaHoubart, Virginie  ; Lecomte, Frédéric ; Cobraiville, Gaël et alPoster (2010, September) Detailed reference viewed: 80 (23 ULg)Penetration mechanism of classical and deformable liposomes through pig ear skinGillet, Aline ; Lecomte, Frédéric ; Hubert, Pascale et alPoster (2010, July 06) Detailed reference viewed: 34 (9 ULg)The experimental design as practical approach to develop and optimize a formulation of peptide-loaded liposomesDucat, Emilie ; ; Lecomte, Frédéric et alin AAPS PharmSciTech (2010), 11(2), 966-975 To investigate the encapsulation of Print 3G, a peptidic agent that could reduce the angiogenic development of breast tumors, pegylated liposomes used as intravenous vectors were studied and characterized ... [more ▼] To investigate the encapsulation of Print 3G, a peptidic agent that could reduce the angiogenic development of breast tumors, pegylated liposomes used as intravenous vectors were studied and characterized. Recently, the path of liposomes has been explored with success to improve the pharmacological properties of peptidic drugs and to stabilize them. In this study, loaded unilamellar vesicles composed of SPC:CHOL:mPEG2000-DSPE (47:47:6) were prepared by the hydration of lipid film technique. An HPLC method was developed and validated for the determination of Print 3G to calculate its encapsulation efficiency. Observed Print 3G adsorption on different materials employed during liposome preparation (such as glass beads, tubing, and connections for extrusion) led to the modification of the manufacturing method. The freeze-thawing technique was used to enhance the amount of Print 3G encapsulated into blank liposomes prepared using the hydration of lipid film procedure. Many factors may influence peptide entrapment, namely the number of freeze-thawing cycles, the lipid concentration, the peptide concentration, and the mixing time. Consequently, a design of experiments was performed to obtain the best encapsulation efficiency while minimizing the number of experiments. The lipid concentration and the number of freeze-thawing cycles were identified as the positive factors influencing the encapsulation. As a result of the optimization, an optimum was found and encapsulation efficiencies were improved from around 30% to 63%. Liposome integrity was evaluated by photon correlation spectroscopy and freeze-fracture electron microscopy to ensure that the selected formulation possesses the required properties to be a potential candidate for further in vitro and in vivo experiments. [less ▲] Detailed reference viewed: 42 (25 ULg) Nouveaux agents anti-inflammatoires en série N-(4-pyridyl)alcanesulfonamideRenard, Jean-François ; Lecomte, Frédéric ; et alConference (2010, May 21) Detailed reference viewed: 64 (19 ULg)Suggested mechanism of action of classical and deformable liposomes through pig ear skinGillet, Aline ; Lecomte, Frédéric ; Rozet, Eric et alConference (2010, April 09) Detailed reference viewed: 48 (16 ULg)Classical and deformable liposomes: possible mechanism of penetration through pig ear skinGillet, Aline ; Lecomte, Frédéric ; Rozet, Eric et alPoster (2010, March 29) Detailed reference viewed: 38 (7 ULg) Optimisation de la séparation des alcaloïdes extraits des feuilles de Strychnos usambarensis au moyen du design spaceNistor, Iolanda ; Debrus, Benjamin ; Lebrun, Pierre et alScientific conference (2010, January 26) Detailed reference viewed: 31 (4 ULg)Développement et validation d'une méthode de dosage de la glucosamine par SPE/LC/MS dans le plamsa caninHubert, Cédric ; Houari, Sabah ; Lecomte, Frédéric et alScientific conference (2010, January 26) Detailed reference viewed: 98 (25 ULg)Intérêt de la chromatographie en mode HILIC pour le dosage du cidofovir dans des fluides biologiquesLecomte, Frédéric ; Hubert, Cédric ; Rozet, Eric et alScientific conference (2010, January 26) Detailed reference viewed: 95 (20 ULg)Development and validation of a sensitive solid phase extraction/hydrophilic interaction liquid chromatography/mass spectrometry method for the accurate determination of glucosamine in dog plasma.Hubert, Cédric ; Houari, Sabah ; Lecomte, Frédéric et alin Journal of Chromatography. A (2010), 1217 A sensitive and accurate LC/MS method was developed for the monitoring of glucosamine (GLcN) dog plasmatic concentration. In this scope, relatively low plasmatic concentrations of GLcN were expected ... [more ▼] A sensitive and accurate LC/MS method was developed for the monitoring of glucosamine (GLcN) dog plasmatic concentration. In this scope, relatively low plasmatic concentrations of GLcN were expected, ranging from 50 to 1000ng/mL. Liquid chromatography coupled to simple quadrupole mass spectrometry detection (LC/MS) was selected bringing the selectivity and the sensitivity needed for this application. Additionally, a solid phase extraction (SPE) step was performed to reduce matrix and ion suppression effects. Due to the ionisable character of the compound of interest, a mixed-mode strong cation exchange (Plexa PCX) disposable extraction cartridge (DEC) was selected. The separation was carried out on a Zorbax SB-CN column (5mum, 4.6mm i.d.x250mm), considering hydrophilic interaction liquid chromatography (HILIC). Indeed, the mobile phase was made of methanol and 5mM ammonium hydrogen carbonate buffer at pH 7.5 (95/5, v/v). The detection was led at m/z ratios of 180.0 and 417.0, for GLcN and IS, respectively. Reliability of the results was demonstrated through the validation of the method using an approach based on the accuracy profile allowing managing the risk associated to the use of these methods in routine analysis: it is thus guaranteed that each future result will fall in the +/-30% acceptance limits with a probability of at least 90%. Successful application of the method to a preliminary pharmacokinetic study illustrated the usefulness of the method for pre-clinical studies. [less ▲] Detailed reference viewed: 149 (56 ULg)Suggested mechanism of action of classical and deformable liposomes through pig ear skinGillet, Aline ; Lecomte, Frédéric ; Rozet, Eric et alin Journal of Pharmacy & Pharmacology (2010), 62(6), 788-807 Detailed reference viewed: 52 (12 ULg)Développement et validation d'une méthode de dosage de la glucosamine par SPE/HILIC/MS dans le plamsa de chienHubert, Cédric ; Houari, Sabah ; Lecomte, Frédéric et alPoster (2009, December 01) Detailed reference viewed: 79 (21 ULg)Intérêt de la chromatographie liquide d'interactions hydrophiles pour l'analyse de composés polaires dans des matrices complexesLecomte, Frédéric ; Demarche, Sophie ; Evrard, Séverine et alConference (2009, December 01) Detailed reference viewed: 157 (29 ULg)Application de la planification expérimentale et du design space pour la séparation des composés extraits des feuilles de Strychnos usambarensisNistor, Iolanda ; Debrus, Benjamin ; Lebrun, Pierre et alPoster (2009, December) Detailed reference viewed: 60 (22 ULg)Développement d’une méthode pour la séparation de 19 antipaludéens par HPLC au moyen de la planification expérimentale et du Design Space; Marini Djang'Eing'A, Roland ; Debrus, Benjamin et alPoster (2009, December) Detailed reference viewed: 77 (21 ULg)Betamethasone in cyclodextrin in deformable liposomes for dermal applications: ex vivo diffusion studiesGillet, Aline ; Lecomte, Frédéric ; Rozet, Eric et alConference (2009, June 09) Detailed reference viewed: 23 (6 ULg)Betamethasone in cyclodextrin in deformable liposomes for dermal applications: ex vivo diffusion studiesGillet, Aline ; Lecomte, Frédéric ; Rozet, Eric et alPoster (2009, June 07) Detailed reference viewed: 16 (2 ULg)Betamethasone in cyclodextrin in deformable liposomes for cutaneous applications: development and diffusion studiesGillet, Aline ; Grammenos, Angeliki ; Lecomte, Frédéric et alConference (2009, May 14) Detailed reference viewed: 20 (2 ULg)Betamethasone in cyclodextrin in deformable liposomes for topical applications: absorption studies using pig skinGillet, Aline ; Lecomte, Frédéric ; Rozet, Eric et alPoster (2009, April 01) Detailed reference viewed: 16 (3 ULg)development and validation of a SPE-LC-UV method for the determination of betamethasone in pig skinGillet, Aline ; Lecomte, Frédéric ; Rozet, Eric et alPoster (2009, March 09) Detailed reference viewed: 28 (6 ULg) |
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