References of "Lassois, Ludivine"
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See detailApple core collection based on SSR data for genome-wide association
Lassois, Ludivine ULg; Durel, Charles-Eric

Scientific conference (2011)

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See detailIdentification of genes involved in the response of banana to crown rot disease
Lassois, Ludivine ULg; Frettinger, Patrick; de Lapeyre de Bellaire, Luc et al

in Molecular Plant-Microbe Interactions [=MPMI] (2011), 24(1), 143-153

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See detailCrown rot of banana: Preharvest factors involved in postharvest disease development and integrated control methods
Lassois, Ludivine ULg; Jijakli, Haissam ULg; Chillet, M. et al

in Plant Disease (2010), 94(6), 648-658

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See detailHand position on the bunch and source-sink ratio influence the banana fruit susceptibility to crown rot disease
Lassois, Ludivine ULg; Bastiaanse, H.; Chillet, M. et al

in Annals of Applied Biology (2010), 156

The postharvest development of crown rot of bananas depends notably on the fruit susceptibility to this disease at harvest. It has been shown that fruit susceptibility to crown rot is variable and it was ... [more ▼]

The postharvest development of crown rot of bananas depends notably on the fruit susceptibility to this disease at harvest. It has been shown that fruit susceptibility to crown rot is variable and it was suggested that this depends on environmental preharvest factors. However, little is known about the preharvest factors influencing this susceptibility. The aim of this work was to evaluate the extent to which fruit filling characteristics during growth and the fruit development stage influence the banana susceptibility to crown rot. This involved evaluating the influence of (a) the fruit position at different levels of the banana bunch (hands) and (b) changing the source–sink ratio (So–Si ratio), on the fruit susceptibility to crown rot. The fruit susceptibility was determined by measuring the internal necrotic surface (INS) after artificial inoculation of Colletotrichum musae. A linear correlation (r = −0.95) was found between the hand position on the bunch and the INS. The So–Si ratio was found to influence the pomological characteristics of the fruits and their susceptibility to crown rot. Fruits of bunches from which six hands were removed (two hands remaining on the bunch) proved to be significantly less susceptible to crown rot (INS = 138.3 mm2) than those from bunches with eight hands (INS = 237.9 mm2). The banana susceptibility to crown rot is thus likely to be influenced by the fruit development stage and filling characteristics. The present results highlight the importance of standardising hand sampling on a bunch when testing fruit susceptibility to crown rot. They also show that hand removal in the field has advantages in the context of integrated pest management, making it possible to reduce fruit susceptibility to crown rot while increasing fruit size. [less ▲]

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See detailIntegrated control of crown rot of banana with Candida oleophila strain O, calcium chloride and modified atmosphere packaging
Bastiaanse, H.; de Lapeyre de Bellaire, Luc; Lassois, Ludivine ULg et al

in Biological Control (2010), 53

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See detailNourrir durablement la planète: Des bananes pour la vie
Lassois, Ludivine ULg

E-print/Working paper (2010)

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See detaille bananier: de la plantation à la récolte
De Clerck, Caroline ULg; Lassois, Ludivine ULg

Diverse speeche and writing (2010)

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See detailLa banane: de la récolte à la consommation
De Clerck, Caroline ULg; Lassois, Ludivine ULg

Diverse speeche and writing (2010)

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See detailL'assainissement viral des bananiers: une étape primordiale dans la préservation de la biodiveristé
Lassois, Ludivine ULg; Castaigne, Michel; D'haes, François

Diverse speeche and writing (2010)

Le laboratoire de Phytopathologie de Gembloux Agro-Bio Tech a développé les techniques permettant la détection et l’assainissement des plantules de bananiers virosés. Ce laboratoire travaille en étroite ... [more ▼]

Le laboratoire de Phytopathologie de Gembloux Agro-Bio Tech a développé les techniques permettant la détection et l’assainissement des plantules de bananiers virosés. Ce laboratoire travaille en étroite collaboration avec Bioversity International afin de fournir au Centre de Transit International, et à fortiori à la communauté internationale, des bananiers indemnes de virus. Les bananiers ainsi obtenus peuvent donc être diffusés largement sans craintes d’une épidémie quelconque. Ce travail d’assainissement contribue non seulement à la sauvegarde de la biodiversité mais également à sa valorisation en rendant saines des variétés qui étaient virosées et non exploitables. Jusqu’à présent l’ITC a fourni 15 000 plantules saines de bananiers dans 335 régions réparties dans 100 pays. Ce travail conjoint de conservation et d’assainissement permet de valoriser la grande diversité génétique du bananier au service de la communauté internationale. [less ▲]

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See detailDes bananes pour la vie
Bay, Daniel ULg; Lassois, Ludivine ULg

Diverse speeche and writing (2010)

Partout à travers le monde, on connait et on aime les bananes. Bien qu’il s’agisse du fruit le plus exporté au monde sa diversité et son importance économique et sociale restent méconnues dans nos pays où ... [more ▼]

Partout à travers le monde, on connait et on aime les bananes. Bien qu’il s’agisse du fruit le plus exporté au monde sa diversité et son importance économique et sociale restent méconnues dans nos pays où elle n’est pas produite. Les bananes constituent la base de la sécurité alimentaire de nombreuses populations. Elles sont consommées principalement sous forme de fruit frais ou comme légume cuit ou frit mais font également l’objet de nombreuses transformations : chips, frites, beignets, purée, confiture, ketchup, alcool, vin, bière,etc. D’autres parties de la plante sont utilisées comme fibre textile, pour la construction d’abris, la fabrication de couvertures, comme combustible ou comme emballages de cuisson. Près de 90% des bananes produites de part le monde sont cultivées par de petits exploitants pour être consommées sur place ou vendues sur les marchés locaux. Le nombre de variétés comestibles est estimé à 1200 et représente une diversité génétique non négligeable. La conservation de cette diversité génétique est primordiale afin de pouvoir répondre aux besoins phytosanitaires, agro-techniques et organoleptiques des différents acteurs de la filière. Que ce soit pour aujourd’hui ou pour demain, pour des bananes d’exportations ou de consommation locale et ce partout à travers le monde. La Belgique : Gardien de la diversité Les bananiers cultivés étant stériles la conservation par graines n’est pas possible. Il est obligatoire de conserver les différentes variétés de bananiers sous forme de plante entière. Cela peut se faire au champ en entretenant les différentes variétés et en sélectionnant les bananiers fils issus de la multiplication végétative, et cela de génération en génération. La conservation peut aussi s’effectuer dans des laboratoires. Dans ce cas les bananiers sont stockés dans des éprouvettes, à l’état de plantules, dans des conditions permettant le ralentissement de leur croissance. La plus grande collection de bananiers au monde se trouve à l’Université Catholique de Leuven (KUL- Laboratory of tropical crop improvement) dans le Centre de Transit International (ITC) de Bioversity International sous les auspices de la FAO (Food and Agriculture Organization) l’organisation des Nations Unies pour l’alimentation et l’agriculture. Cette collection, inaugurée en 1985, est toujours en expansion et compte aujourd’hui plus de 1000 accessions différentes de bananiers. Dès qu’une nouvelle variété est découverte, elle est envoyée dans ce centre afin de garantir sa conservation. Les plantules conservées à l’ITC peuvent être multipliées et expédiées dans différents pays. Cependant, avant de distribuer ces plantes à l’extérieur du centre il est nécessaire de vérifier que celles-ci sont indemnes de virus et de tout autre organisme pathogène. Le laboratoire de Phytopathologie de Gembloux Agro-Bio Tech a développé les techniques permettant la détection et l’assainissement des plantules de bananiers virosés. Ce laboratoire travaille en étroite collaboration avec Bioversity International afin de fournir au Centre de Transit International, et à fortiori à la communauté internationale, des bananiers indemnes de virus. Les bananiers ainsi obtenus peuvent donc être diffusés largement sans craintes d’une épidémie quelconque. Ce travail d’assainissement contribue non seulement à la sauvegarde de la biodiversité mais également à sa valorisation en rendant saines des variétés qui étaient virosées et non exploitables. Jusqu’à présent l’ITC a fourni 15 000 plantules saines de bananiers dans 335 régions réparties dans 100 pays. Ce travail conjoint de conservation et d’assainissement permet de valoriser la grande diversité génétique du bananier au service de la communauté internationale. [less ▲]

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See detailLe bananier: comment obtenir du matériel de plantation indemne de virus
De Clerck, Caroline ULg; Lassois, Ludivine ULg

Diverse speeche and writing (2010)

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See detailLa banane: de son origine à sa commercialisation
Lassois, Ludivine ULg; Busogoro, J. P.; Jijakli, Haissam ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2009), 13(4), 575-586

Cultivated bananas are giant herbaceous plants within the genus Musa. They are both sterile and parthenocarpic. There are well over a thousand domesticated Musa cultivars, they are mostly triploid (a few ... [more ▼]

Cultivated bananas are giant herbaceous plants within the genus Musa. They are both sterile and parthenocarpic. There are well over a thousand domesticated Musa cultivars, they are mostly triploid (a few are diploid or tetraploid) and are derived from crosses between two wild species, Musa acuminata and Musa balbisiana. In terms of production, bananas are the fourth agricultural product after rice, wheat, and maize. They constitute the basis of food security for many people. Cropping systems vary widely around the world and contrasting objectives are encountered: consumption by the producer, sale on local or national markets, export, etc. Cooking bananas, including plantains, must be distinguished from dessert bananas, which constitute a major international trade. This international trade started only in the early 1900s but it has since grown continuously. Banana is currently the most exported fruit, in terms of both value and quantity. Despite the high genetic diversity found within the genus Musa, the export market is mainly based on single Cavendish. There are major challenges to banana production from biotic or abiotic stresses to continue to meet the criteria of sustainability, quality and yield that are imposed. [less ▲]

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See detailCombining an original method for preserving RNA expression in situ with an effetive RNA method makes it possible to study gene expression in any banana fruit tissue.
Lassois, Ludivine ULg; de Lapeyre de Bellaire, Luc; Jijakli, Haissam ULg

in Fruits (2009), 64(3), 127-137

Introduction. RNA isolation is a prerequisite to studying gene expression in banana and to understanding changes occurring in response to the environment. Standard extraction methods do not efficiently ... [more ▼]

Introduction. RNA isolation is a prerequisite to studying gene expression in banana and to understanding changes occurring in response to the environment. Standard extraction methods do not efficiently extract RNA from plants such as banana, with high levels of phenolics, carbohydrates, or other compounds that bind to and/or coprecipitate with RNA. Materials and methods. Five to seven RNA extraction methods were compared. Four crowntissue storage methods were also compared. cDNA-AFLP was used to ensure that the obtained RNA was of sufficient quality for molecular applications and that RNA expression was unaltered by in situ storage. Results and discussion. The modified hot-borate method proved to be the best RNA extraction method, allowing high yields of good quality, undegraded RNA from the crown, fruit peel and pulp at all stages of ripening. The RNA obtained by this method was of sufficient quality for molecular applications such as cDNA-AFLP that give highly reproducible results. Freeze-drying of fresh tissues and tissue conservation in hot-borate buffer, two original storage methods, appear appropriate for preserving RNA in situ without ultra-low temperature. The RNA obtained was of high quality, undegraded, and useful for all downstream applications. The genome expression profile obtained by cDNA-AFLP analysis was unaltered by these methods for storing collected tissues. Conclusion. By applying all the suggested procedures in this work, it is possible to store and study gene expression in any banana fruit tissue, whatever the maturity stage, without affecting the RNA expression level. [less ▲]

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See detailThe post-harvest quality of bananas is determined by pre-harvest factors
de Lapeyre de Bellaire, Luc; Chillet, Marc; Lassois, Ludivine ULg et al

Poster (2009)

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See detailAgronomical and molecular factors influencing bananas (Musa acuminata, AAA, cv ‘Grande-Naine’) susceptibility to crown rot disease
Lassois, Ludivine ULg

Doctoral thesis (2009)

Crown rot affects export bananas in all producing countries and is considered to be one of the main export banana post-harvest disease. Variations are observed in the expression of crown rot symptoms. An ... [more ▼]

Crown rot affects export bananas in all producing countries and is considered to be one of the main export banana post-harvest disease. Variations are observed in the expression of crown rot symptoms. An original approach of the disease is proposed and consists on presenting the fruit quality potential at harvest as a key factor in crown rot development. This potential develops during growth of bananas in the field and depends on a physiological and a parasitical component. The physiological component refers here to the level of fruit susceptibility to crown rot and reflects the physiological state of the fruit. The aim of this study was to clarify the role of the fruit physiological component at harvest in the post-harvest crown rot development. It appears that the fruit physiological component at harvest greatly influence the postharvest disease development and thus the fruit susceptibility. Seasonal variations in disease severity were shown in two production area and are related to a variation of the fruit physiological component. In Guadeloupian conditions, the internal necrotic surface of the crown was nearly multiplied by 4 during 11 successive weeks. Two pre-harvest factors that could influence the fruit physiological component by modifying their susceptibility to crown rot, were identified: (i) hand position on the bunch and (ii) source-sink ratio of the banana plant (hand considered as sink and leaves as source). It was shown that within a bunch, there is a gradient of susceptibility to crown rot (r= -0.95), the hands initiated first (the upper ones) being more susceptible than those initiated last (the lower ones). These results also confirmed that source-sink ratio changes have a significant effect on fruit morphology and demonstrated that there is also an effect on fruit susceptibility to crown rot disease. When the sink is decreased by artificial removal of many hands, the level of fruit susceptibility to crown rot decreases. However, the molecular mechanisms underlying these quantitative host-pathogen relationships were still unknown. A study was designed to compare gene expression, by cDNA-AFLP, between crowns of bananas showing a high susceptibility (S+) and crowns of bananas showing a low susceptibility (S-) to Colletotrichum musae responsible for crown rot disease. This comparison was performed at two situation time: (i) between crowns (S+ and S-) collected one hour before infection and (ii) between crowns (S+ and S-) collected 13 days after infection. Genes implied in signaling pathway and proteolytic machinery were identified. It also appears that a cellulose synthase, a CAF1 gene, 2 glycolipid transfer protein and a dopamine-β-monooxygenase were differently expressed between bananas showing different levels of susceptibility. This is the first study of the characterization of the banana physiological component at harvest which influences the crown rot post-harvest disease development. In addition, to our knowledge, this work is the first to address both pre- and post-infection gene expression with the same host-pathogen combination and different susceptibility levels. [less ▲]

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