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See detailLes peptides natriurétiques: le point de vue du biologiste
LE GOFF, Caroline ULiege

Conference (2015, February 26)

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See detailLe dosage de la vitamine D: considérations pré-analytiques et analytiques
LE GOFF, Caroline ULiege; Souberbielle, Jean-Claude; Delvin, Edgard et al

in Annales de Biologie Clinique (2015), 73(1), 79-92

The constantly increasing requests for the measurement of serum 25-hydroxyvitamin D over the last years has led reagent manufacturers to market different automated and semi-automated methods, that being ... [more ▼]

The constantly increasing requests for the measurement of serum 25-hydroxyvitamin D over the last years has led reagent manufacturers to market different automated and semi-automated methods, that being unfortunately not fully harmonized, yield different results. Liquid chromatography coupled to tandem mass spectrometry has more recently been introduced. This approach allows the distinction between the two forms of 25-hydroxyvitamin D and to measure other metabolites. This approach also requires harmonization to curtail the differences between the different analytical methods. To meet this requirement, the American national institutes of health (NIH), the CDC (Center for disease control and prevention) in Atlanta, the NIST (National institute of standards and technology) and the vitamin D Reference laboratory of Ghent University have pooled their expertise to develop a standardization program. This article reviews the main elements and the difficulties of the automated and semi-automated methods for 25-hydroxyvitamin D, from sample preparation to the analytical phase, as well as those related to mass spectrometry. It also addresses the issues related to the clinical decision thresholds and the possibility of measurements in different biological liquids. [less ▲]

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See detailEpidemiological Review of Injuries in Rugby Union
Kaux, Jean-François ULiege; Julia, Marc; Delvaux, François ULiege et al

in Sports (2015), 3(1), 21-29

Rugby is a sport that is growing in popularity. A contact sport par excellence, it causes a significant number of injuries. In Rugby Union, there are 30 to 91 injuries per 1000 match hours. This ... [more ▼]

Rugby is a sport that is growing in popularity. A contact sport par excellence, it causes a significant number of injuries. In Rugby Union, there are 30 to 91 injuries per 1000 match hours. This epidemiological review of injuries incurred by rugby players mentions the position and type of injuries, the causes, time during the match and season in which they occur and the players’ positions as well as the length of players’ absences following the injury. [less ▲]

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See detailElevated Plasma Soluble ST2 Is Associated with Heart Failure Symptoms and Outcome in Aortic Stenosis.
LANCELLOTTI, Patrizio ULiege; DULGHERU, Raluca Elena ULiege; Magne, Julien et al

in PloS one (2015), 10(9), 0138940

B-type natriuretic peptide (BNP) is often used as a complementary finding in the diagnostic work-up of patients with aortic stenosis (AS). Whether soluble ST2, a new biomarker of cardiac stretch, is ... [more ▼]

B-type natriuretic peptide (BNP) is often used as a complementary finding in the diagnostic work-up of patients with aortic stenosis (AS). Whether soluble ST2, a new biomarker of cardiac stretch, is associated with symptomatic status and outcome in asymptomatic AS is unknown. sST2 and BNP levels were measured in 86 patients (74+/-13 years; 59 asymptomatic, 69%) with AS (<1.5 cm2) and preserved left ventricular ejection fraction who were followed-up for 26+/-16 months. Both BNP and sST2 were associated with NYHA class but sST2 (>23 ng/mL, AUC = 0.68, p<0.01) was more accurate to identify asymptomatic patients or those who developed symptoms during follow-up. sST2 was independently related to left atrial index (p<0.0001) and aortic valve area (p = 0.004; model R2 = 0.32). A modest correlation was found with BNP (r = 0.4, p<0.01). During follow-up, 29 asymptomatic patients (34%) developed heart failure symptoms. With multivariable analysis, peak aortic jet velocity (HR = 2.7, p = 0.007) and sST2 level (HR = 1.04, p = 0.03) were independent predictors of cardiovascular events. In AS, sST2 levels could provide complementary information regarding symptomatic status, new onset heart failure symptoms and outcome. It might become a promising biomarker in these patients. [less ▲]

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See detailHTA d’origine endocrinienne : l’aide du laboratoire
LE GOFF, Caroline ULiege

Conference (2014, October 01)

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See detailIntérêt d’une seconde infiltration de plasma riche en plaquettes dans le traitement des tendinopathies patellaires ?
Kaux, Jean-François ULiege; Croisier, Jean-Louis ULiege; Buhler, Frédéric et al

in 7ème Congrès National SFMES & SFTS 2014 (2014, September)

Introduction : Des études cliniques ont évalué l’effet du plasma riche en plaquettes (PRP) dans le traitement de la tendinopathie patellaire. Même si une seule infiltration peut améliorer les symptômes du ... [more ▼]

Introduction : Des études cliniques ont évalué l’effet du plasma riche en plaquettes (PRP) dans le traitement de la tendinopathie patellaire. Même si une seule infiltration peut améliorer les symptômes du sportif dans cette indication, la majorité des études évaluent les effets de 3 infiltrations successives. Le but de notre étude était donc de comparer l’efficacité d’une et de 2 infiltrations (rapprochées) de PRP dans le cade de tendinopathies patellaires chroniques, rebelles aux traitements conservateurs. Matériel et méthode : Vingt sportifs souffrant de tendinopathie patellaire depuis plus de 3 mois ont été inclus et randomisés en 2 groupes, respectivement 1 ou 2 infiltrations de PRP (15 jours d’intervalle). Le PRP a été obtenu à l’aide d’une machine d’aphérèse permettant d’obtenir une concentration plaquettaire identique pour tous les sujets. Ils ont bénéficié ensuite d’une rééducation excentrique sous-maximale standardisée. Le suivi a été réalisé à l’aide d’une échelle visuelle analogique de la douleur, de scores algo-fonctionnels (IKDC et VISA-P), ainsi qu’un questionnaire « mode de vie » avant l’infiltration, 3 et 12 mois post-infiltration. Résultats: La concentration de PRP employée était la même au sein des 2 groupes (9x106/µL), et ne contenait pas d’érythrocyte ni de leucocyte. Au terme du suivi (1 an), les résultats montrent une amélioration des scores EVA dans les deux groupes et significative pour le groupe 1 (p=0,005). Les scores VISA-P et IKDC ont tous deux augmentés, significativement dans les deux groupes. Un sujet par groupe n’a pas repris d’activité physique tandis 67% ont repris le sport au niveau antérieur dans le groupe 1 et 78% dans le groupe 2. Conclusion: La comparaison entre 1 et 2 infiltrations rapprochées de PRP n’a pas permis de mettre en évidence de différence entre les 2 groupes sur un suivi d’un an. Une seconde infiltration rapprochée ne semble donc pas indiquée pour améliorer l’efficacité de ce traitement. Cependant, un suivi à plus long terme serait nécessaire. De même l’évaluation d’une seconde infiltration plus à distance (3 mois) de la première mériterait d’être explorée. [less ▲]

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See detailComparaison du VeinoPlus Sport et du TENS sur la récupération de jeunes footballeurs
God, Maxime; LE GOFF, Caroline ULiege; Evrard, Frédéric et al

in 7ème Congrès National SFMES & SFTS 2014 (2014, September)

Introduction : Toute activité physique fatigue l’organisme ; les muscles produisent des métabolites et de d’acide lactique, limitant leur fonctionnement. Il est donc nécessaire de pouvoir récupérer de ... [more ▼]

Introduction : Toute activité physique fatigue l’organisme ; les muscles produisent des métabolites et de d’acide lactique, limitant leur fonctionnement. Il est donc nécessaire de pouvoir récupérer de manière efficace au plus vite. En effet, une mauvaise récupération porte préjudice à la performance et à l’état physique du sportif. Aux côtés de la récupération active et de la balnéothérapie de nouvelles techniques comme de la cryothérapie et électrostimulation ont fait leur apparition pour une meilleure récupération. Le but de cette étude était de comparer l’impact de 2 types de stimulateurs : « VeinoPlus Sport » (VPS) et « TENS » sur la récupération des footballeurs amateurs après un effort de type Yo-Yo. Matériel et méthode : Dans un contexte identique, chaque sportif (20 footballeurs; âge moyen = 17,1 ans ± 0,79) a bénéficié du VPS ou du TENS lors de 2 séances différentes après 2 efforts de type Yo-Yo, séparés d’une période de récupération. Quatre prélèvements sanguins (lactate, gaz sanguins, ions, marqueurs cardiaques…) ainsi qu’un évaluation de l’explosivité par Myotest ont été réalisés respectivement avant l’épreuve, juste après, après la récupération et après la seconde épreuve. Un questionnaire relatif à l’état de fatigue a également été rempli par les sujets. Pour l’analyse statistique, nous avons utilisé le logiciel « StatPlus » et réalisé une Anova à mesures répétées ainsi qu’un test de Scheffé. Résultats : Les sportifs ont tous parcouru une plus grande distance lors des 2èmes efforts, surtout au sein du groupe VPS, mais sans différence significative. L’épreuve a déclenché une variation significative de lactatémie, du HCO3- ainsi que de la myoglobine. L’étude des autres paramètres sanguins ainsi que des paramètres de l’explosivité n’a révélé aucune évolution significative tout au long de l’étude. Par contre en ce qui concerne les critères subjectifs (« ressenti »), les footballeurs du groupe VPS ont noté, à 12h et 24h, une moindre douleur aux jambes, une moindre sensation de fatigue, une moindre diminution de la force, une plus grande légèreté ainsi qu’un meilleur état de forme par rapport au groupe TENS. Enfin, en général, et sans noter de préférence particulière pour l’un ou l’autre des appareils, les athlètes ont préféré l’utilisation de l’électrostimulation à leur récupération habituelle. Conclusion : Cette étude ne montre que des différences mineures, non significatives, entre les deux appareils. Les 2 groupes ont parcouru une distance plus grande lors du 2ème effort. Ceci suggère que le test n’était pas épuisant, malgré les signes biologiques de « fatigue ». Cependant, sur des critères subjectifs de récupération et de bien–être, une tendance était en faveur du VPS. Ces résultats seront à prendre en compte pour de nouvelles études avec cette fois des tests plus fatigants. [less ▲]

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See detailGalectin-3: a new promising cardiac biomarker in sports endurance?
LE GOFF, Caroline ULiege; Devaux, Séverine; BREVERS, Eric ULiege et al

in Cardiovascular Research (2014, July), 103(Supplement 1), 255

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See detailURINARY AND SALIVARY CORTISOL IN LIQUID CHROMATOGRAPHY–TANDEM MASS SPECTROMETRY: METHOD VALIDATION AND EXPECTED VALUES DETERMINATION
LE GOFF, Caroline ULiege; DELCOUR, Sandra ULiege; PEETERS, Stéphanie ULiege et al

in Clinical Chemistry & Laboratory Medicine (2014, June), 52(Special Suppl), 1241

BACKGROUND: Cortisol measurement is useful in evaluation of Cushing syndrome, adrenal insufficiency, mineralocorticoid excess and congenital adrenal hyperplasia. We developed a liquid ... [more ▼]

BACKGROUND: Cortisol measurement is useful in evaluation of Cushing syndrome, adrenal insufficiency, mineralocorticoid excess and congenital adrenal hyperplasia. We developed a liquid chromatography–tandem mass spectrometry (LCMS/MS) method for salivary and urinary cortisol and we determined the 95th percentile (p95) for the urinary and salivary cortisol. We compared them to the Mayo Clinic expected values. METHODS: Saliva at 8 am and 11 pm and 24h urine were obtained from 32 healthy (22 female, 34.3±9.3 yo) volunteers. We performed validation with the enoval software (Arlenda, Belgium). For the validation, we used water or urine with spiked known amounts of cortisol for the CORS and CTU respectively. For the CORS, samples were centrifuged, deuterium labelled cortisol was added as internal standard and the protein precipated by acetonitril. The supernatant was evaporated, dissolved in methanol acidified with acetic acid and analyzed by LCMS/MS. For CTU, samples were centrifuged, deuterium labelled cortisol was added as internal standard and diluted by the ammonium acetate and analyzed by LCMS/MS. At the Mayo Clinic, the expected values were 1-7.5 μg/L (7 a.m-9 a.m) and <1 μg/L (11-12 p.m) for CORS and 3.5-45 μg/24h (<18yo) for CTU. RESULTS: For the CTU, the with-in run did not exceed 3% (0.4-3%) and the between-run did not exceed 3.1% (0.4-3.1%) for 1.5-750 μg/L. The limit of quantification was 1.5 μg/L. The linearity was good between 1.5 and 750 μg/L. The recovery is 97.9±2.2% (95%CI for the mean: 92.4-101.1%). For the CORS, the with-in run and between run did not exceed 8% (1.9-8%) for 1.15-8.65 μg/L. The limit of quantification was 1.15 μg/L. The analyse presents a good linearity between 1.15 and 8.65 μg/L. The recovery is 99.9±2.9% (95%CI for the mean: 94.2-108.7%). The p95 for the CTU according to the CLSI C28-A3 was 33 μg/24h, and for the CORS was 5.42 μg/L at 8 am and 0.7 μg/L at 12 pm. CONCLUSIONS: Our developed method in liquid chromatography tandem mass spectrometry was validated for the measurement of urinary and salivary cortisol. Our findings indicate that the proposed analytical methods were suitable for routine purposes and useful in many pathological conditions.The expected values confirm these defined by the Mayo Clinic. [less ▲]

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See detailEvaluation of troponin T on AQT90 Flex and COBAS 8000 as a rule in/out tool in an emergency ward
LE GOFF, Caroline ULiege; EVRARD, Séverine ULiege; BREVERS, Eric ULiege et al

in Clinical Chemistry & Laboratory Medicine (2014, June), 52(Special Suppl), 510

BACKGROUND: Troponin measurement is the gold standard for diagnosis of Acute Myocardial Infarction (AMI). Troponin (highly sensitive (hs), T or I) is measured by immunochemistry instrument or by Point of ... [more ▼]

BACKGROUND: Troponin measurement is the gold standard for diagnosis of Acute Myocardial Infarction (AMI). Troponin (highly sensitive (hs), T or I) is measured by immunochemistry instrument or by Point of Care (POCT). POCT can be useful in emergency lab or ward for a faster diagnosis of patients with chest pain. Our study compared analytical performance of a POCT AQT90 Flex (Radiometer Medical) (AQT) and TnThs Cobas 8000 (Roche Diagnostics) (Cobas). We also compared the clinical performance of both methods at recommended cut-off (14 ng/L for Cobas and 30 ng/ L for AQT). METHODS: We selected 104 patients (296 samples) (range: 6-13822 ng/L) admitted in the Emergency ward for which at least 1 troponin determination (Cobas 8000) had been re-quested in the past 24 hours according to rule in/out procedure applied by this ward. Samples were then measured with the AQT. Inter-assay CV was maximum 8.6% and 9.6% for Cobas and AQT respectively. The cut-off defined as the 99th percentile for Roche was 14 ng/L and the recommended decision threshold value was 30 ng/L for Radiometer. Retrospective analysis of final diagnostic was obtained for all participants: we considered as “true positive” patients for whom a final diagnostic was ST segment-Elevation Myocardial Infarction (STEMI) or non STEMI (NSTEMI). RESULTS: On the whole range of measure, the 2 methods showed a good correlation (r2=0.98). Regression equation was Cobas = 0.98 AQT + 31 ng/L (95%CI of the intercept: (26.7;37.7) and 95% CI of the slope (0.96;1)). When we stratified, for the values <54 ng/L, the equation became Cobas = 0.52 AQT +1.1 ng/L (95%CI of the intercept: (-4.8;5.5) and 95% CI of the slope (0.39;0.69)). Bland and Altman plot did not show any bias. At admission [2-7 hours], 78 (81%) of admitted patients were finally considered as AMI, sensitivity was 92 % [96%] for Cobas and 78% [91%] for AQT. Specificity was 15% for Cobas (cut-off 14ng/L) or 73% (cut-off 54 ng/L) and 76% for AQT. CONCLUSIONS: Overall, there was a good correlation between the 2 methods. However, using a cut-off of 14 ng/L for Cobas is questionable for a rule in/out procedure in an emergency ward. Using 54 ng/L for Roche and 30 ng/L for AQT would have led to the best discrimination between patients presenting AMI or not. [less ▲]

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See detailPlatelet-rich plasma to treat patellar tendinopathies: a 1 year follow-up.
Kaux, Jean-François ULiege; Croisier, Jean-Louis ULiege; Bruyère, Olivier ULiege et al

in Clinical Chemistry & Laboratory Medicine (2014, June), 52(Special Suppl), 1285

Background: Jumper’s knee is a frequent chronic overuse syndrome of the upper part of the patellar tendon. Platelets contain lots of growth factors which could enhance the healing process of tendons ... [more ▼]

Background: Jumper’s knee is a frequent chronic overuse syndrome of the upper part of the patellar tendon. Platelets contain lots of growth factors which could enhance the healing process of tendons. Infiltration of Platelet Rich Plasma (PRP) may be considered as a recent therapeutic option for chronic tendinopathies. The aim of the current study is to evaluate the clinical status and the return to sports activities in patients with chronic upper patellar tendinopathies up to 1 year after 1 infiltration of PRP. Methods: Twenty patients with chronic upper patellar tendinopathy were enrolled. Assessments were made before infiltration of PRP, and 6 weeks and 3 months and 1 year after the infiltration, using a 10-point Visual Analogic Scale and algofunctional scores (IKDC and VISA-P). Moreover, they had to answer an information questionnaire concerning their life and sports activities. The PRP was obtained with an apheresis system (COM.TEC, Fresenius). Six millilitres of PRP were injected without local anaesthetic. One week after infiltration, patients started a standardised sub-maximal eccentric reeducation. Results: Pain during daily activities significantly decreased with time. VAS has significantly dropped, IKDC and VISA-P significantly improved over the follow-up of 1 year. Seventy percents of patients reported a favourable evolution with decrease of pain, 15% did never report any improvement and 15% were treated surgically. Seventy percents returned to sports activities, 64,3% without any pain, and 50% of them recovered the same sport level. Younger patients seemed to be more susceptible to have an improvement of pain by the PRP infiltration. Conclusions: This study demonstrates that a local infiltration of PRP associated with a submaximal eccentric protocol can improve, at 1 year, symptoms of chronic jumper’s knee in patients non-responsive to classical conservative treatments. [less ▲]

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See detailSUITABILITY OF 24,25(OH)2 VITAMIN D3 DETERMINATION WITH AN ADAPTED VERSION OF THE CHROMSYSTEMS® MASSCHROM® 25-OH-VITAMIN D3/D2– LC-MS/MS KIT
SCHLECK, Marie-Louise ULiege; NETCHACOVITCH, Matthieu ULiege; CRINE, Yannick ULiege et al

in Clinical Chemistry & Laboratory Medicine (2014, June), 52(Special Suppl), 1235

BACKGROUND: The enzym CYP24A1 catalyses the conversion of 25(OH)D3 in 24,25(OH)2D3. Recently, loss-of-function mutation of CYP24A1 has been identified in idiopathic infantile hypercalcemia (IIH). This ... [more ▼]

BACKGROUND: The enzym CYP24A1 catalyses the conversion of 25(OH)D3 in 24,25(OH)2D3. Recently, loss-of-function mutation of CYP24A1 has been identified in idiopathic infantile hypercalcemia (IIH). This genetic defect can be highlighted by high 1,25(OH)2D3 and undetectable 24,25(OH)2D3 levels. 24,25(OH)2D3 is also known to interfere with 25(OH)D3 determinations with immunoassays, leading to an overestimation of the 25(OH)D3 concentrations. We adapted the MassChrom kit on the AB SCIEX TQ 5500 in order to systematically provide, next to 25(OH)D3, 25(OH)D2 and C3 epimer, the concentrations of 24,25(OH)2D3. The aim of this study was to evaluate the performance of 24,25(OH)2D3 determination with this modified method. We also wanted to establish the reference value of 24,25(OH)2D3. METHODS: We modified the Chromsystems MassChrom method by adding the 24,25(OH)D3 correspondent transitions and performed a calibration by spiking known amounts of 24.25(OH)2D3. The LOQ was determined with 10 concentration levels of 24,25(OH)2D3. We selected 92 healthy children (40 girls; 2.4±1.51 years) presenting normal calcium levels (2.49±0.13mmol/l) to determine the 95th percentile (p95). RESULTS: The 24,25(OH)2D3 LOQ was 4.7 ng/ml. 85.9% of our subjects were below this LOQ. The p 95 for the 24,25(OH)2D3, according to the CLSI C28-A3, was <6.2 ng/ml. The average serum concentrations (mean±SD) of 25(OH)D3 and 3-epi-25(OH)D3 were 24.48±10.22ng/ml and 2.07±1.86 ng/ml respectively. The 24,25(OH)2D3 levels (r2=0.64) correlated with the 25(OH)D3 levels. CONCLUSIONS: Our adapted method from Chromsystems Vitamin D determination is available to quantify 24,25(OH)2D3. In this context, this method is able to determine high levels of 24,25(OH)2D3 that can possibly cross react with immunoassays. However, as the LOQ was not low enough, we couldn’t establish correct reference value for 24,25(OH)2D3. A derivatization step in the sample preparation would be interesting to improve the sensibility of the method. [less ▲]

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See detailVascular Endothelial Growth Factor-111 (VEGF-111) and tendon healing: preliminary results in a rat model of tendon injury
Kaux, Jean-François ULiege; Janssen, Lauriane ULiege; Drion, Pierre ULiege et al

in Muscles, Ligaments and Tendons Journal (2014), 4(1 (eCollection 2014 Jan)), 25-28

Tendon lesions are among the most frequent musculoskeletal pathologies. Vascular endothelial growth factor (VEGF) is known to regulate angiogenesis. VEGF-111, a biologically active and proteolysis ... [more ▼]

Tendon lesions are among the most frequent musculoskeletal pathologies. Vascular endothelial growth factor (VEGF) is known to regulate angiogenesis. VEGF-111, a biologically active and proteolysis-resistant splice variant of this family, was recently identified. This study aimed at evaluating whether VEGF-111 could have a therapeutic interest in tendon pathologies. Surgical section of one Achilles tendon of rats was performed before a local injection of either saline or VEGF-111. After 5, 15 and 30 days, the Achilles tendons of 10 rats of both groups were sampled and submitted to a biomechanical tensile test. The force necessary to induce tendon rupture was greater for tendons of the VEGF-111 group (p<0.05) while the section areas of the tendons were similar. The mechanical stress was similar at 5 and 15 days in the both groups but was improved for the VEGF-111 group at day 30 (p <0.001). No difference was observed in the mRNA expression of collagen III, tenomodulin and MMP-9. In conclusion, we observed that a local injection of VEGF-111 improves the early phases of the healing process of rat tendons after a surgical section. Further confirmatory experimentations are needed to consolidate our results. [less ▲]

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See detailIs isokinetic eccentric exercise dangerous for the heart?
LE GOFF, Caroline ULiege; Kaux, Jean-François ULiege; LAURENT, Terry ULiege et al

in Isokinetics & Exercise Science (2014), 22(2), 131-136

BACKGROUND: Very strenuous exercises can be performed on an isokinetic dynamometer in order to evaluate the resistance to fatigue of different muscular groups. Good cardiac function is necessary in order ... [more ▼]

BACKGROUND: Very strenuous exercises can be performed on an isokinetic dynamometer in order to evaluate the resistance to fatigue of different muscular groups. Good cardiac function is necessary in order to perform these very intensive exercises; otherwise an acute myocardial dysfunction could theoretically appear in predisposed patients. OBJECTIVE: Our study aimed to observe the cardiovascular impact of a maximal intense isokinetic eccentric exercise performed by a population of sedentary young men. METHODS: Resting and post-exercise (just after, 3 hours and 24 hours after the exercise) blood samples were taken from 12 young male sedentary healthy subjects. These subjects performed an intense maximal eccentric isokinetic exercise of the quadriceps muscle.We evaluated markers of cardiovascular risk (troponin I, highly sensitive troponin T, NT-proBNP,myoglobin), of inflammation (hsCRP) and of oxidative stress (myeloperoxydase, lipidic peroxides, reduced and oxidized glutathione). RESULTS: The following observations were made: no significant increase in cardiac (NT-proBNP, troponins) or inflammation (hsCRP) biomarkers; a significant increase in myoglobin, myeloperoxidase, lipidic peroxides, oxidised glutathione just after the exercise. CONCLUSIONS: No modification in cardiac biomarkers were observed after the maximal eccentric isokinetic exercise. We were thus able to prove that the exercise could be performed without any risk to cardiac function in young sedentary subjects. However, a significant level of oxidative stress was induced by this exercise. [less ▲]

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See detailNo interest for a second close infiltration of platelet-rich plasma to treat upper patellar tendinopathies
Kaux, Jean-François ULiege; Croisier, Jean-Louis ULiege; Buhler, Frédéric et al

in European Journal of Physical and Rehabilitation Medicine (2014, May), 50(Suppl. 1 to No. 3), 21639-002-

Background: Some clinical series have evaluated the effect of platelet-rich plasma (PRP) in the treatment of jumper’s knee. Although it is possible that a single infiltrative administration may prove to ... [more ▼]

Background: Some clinical series have evaluated the effect of platelet-rich plasma (PRP) in the treatment of jumper’s knee. Although it is possible that a single infiltrative administration may prove to be an effective treatment for this indication, most of the existing studies evaluated the effects of 2 or 3 successive infiltrations. The aim of this study was to evaluate whether 2 infiltrations of PRP proves more effective than a single treatment. Methods: Twenty patients suffering from jumper’s knee for over than 3 months were enrolled into the study and split into two randomized groups (1 or 2 infiltrations of PRP, respectively). The follow-up evaluation consisted of VAS, IKDC and VISA-P scores, along with algometer, isokinetic and ultrasounds evaluations. Results: The concentration of the PRP used for each infiltration was similar in both groups, and contained no red or white cells. Results revealed no difference in treatment efficacy between the groups. Conclusion: The comparison between 1 or 2 infiltrations of PRP did not reveal any difference between the 2 groups after a follow-up period of 3 months. A second closely-timed infiltration of PRP to treat jumper’s knees is not necessary to improve the efficacy of this treatment in the short term. [less ▲]

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See detailOne-year follow-up of platelet-rich plasma to treat chronic upper patellar tendinopathies
Kaux, Jean-François ULiege; Croisier, Jean-Louis ULiege; Bruyère, Olivier ULiege et al

in European Journal of Physical and Rehabilitation Medicine (2014, May), 50(Suppl. 1 to No. 3), 227250-

Introduction: Infiltration of PRP may be used as a recent therapeutic option for chronic tendinopathies The aim of the current study is to evaluate the clinic and the return to sports activities in ... [more ▼]

Introduction: Infiltration of PRP may be used as a recent therapeutic option for chronic tendinopathies The aim of the current study is to evaluate the clinic and the return to sports activities in patients with chronic upper patellar tendinopathies 1 year after 1 infiltration of PRP. Material and methods: The follow-up of 20 subjects who beneficed from 1 infiltration of PRP was made before infiltration, after 3 months and 1 year after infiltration; it was made as follow: VAS, IKDC and VISA-P scores. Moreover, they had to answer an information questionnaire concerning their life and sports activities. Results: Seventy percents of patients reported a favourable evolution with decrease of pain, 10% did never report any improvement and 20% were treated surgically. Eighty-seven percents returned to sports activities without any pain, and 50% of them recovered the same sport level. VAS has significantly (p<0.0001) dropped, IKDC significantly improved (p=0.0007) and VISA-P also significantly increased (p=0.0087) over the follow-up of 1 year. Discussion: This study confirms that a local injection of PRP coupled with a program of eccentric rehabilitation through a chronic Jumper's knee, improves painful symptoms and the functionality of the subjects’ knee up to a follow-up of 1 year. [less ▲]

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