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See detailEccentric training improves tendon biomechanical properties: a rat model
Kaux, Jean-François ULg; Drion, Pierre ULg; Libertiaux, Vincent et al

in Journal of Orthopaedic Research (2013), 31(1), 119-124

Introduction: The treatment of choice for tendinopathies is eccentric reeducation. Although the clinical results appear favourable, the biomechanical changes to the tissue are not yet clear. Even if the ... [more ▼]

Introduction: The treatment of choice for tendinopathies is eccentric reeducation. Although the clinical results appear favourable, the biomechanical changes to the tissue are not yet clear. Even if the mechanotransduction theory is commonly accepted, the physiology of tendons is not clearly understood. We aimed to better define the biomechanical and histological changes that affect healthy tendon after eccentric and concentric training. Materiel and Methods: This study compared the effects of 2 methods of training (eccentric (E) training and concentric (C) training) with untrained (U) rats. The animals were trained over a period of 5 weeks. The tricipital, patellar and Achilles tendons were removed, measured and a tensile test until failure was performed. A histological analysis (hematoxylin and eosin and Masson's trichrome stains) was also realized. Results: There was a significant increase in the rupture force of the patellar and tricipital tendons between the U and E groups. The tricipital tendons in the control group presented a significantly smaller cross-sectional area than the E- and C-trained groups, but none was constated between E and C groups. No significant difference was observed for the mechanical stress between the three groups for all three tendons. Histological studies demonstrated the development of a greater number of blood vessels and a larger quantity of collagen in the E group. Discussion and conclusion: The mechanical properties of tendons in rats improve after specific training, especially following eccentric training. Our results partly explained how mechanical loading, especially in eccentric mode, could improve the healing of tendon. [less ▲]

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See detailIdentification of protein biomarkers associated with cardiac ischemia by a proteomic approach.
Fillet, Marianne ULg; Deroyer, Céline ULg; COBRAIVILLE, G. et al

in Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals (2013), 18(7), 614-24

Angina is chest pain induced by ischemia of the heart muscle, generally due to obstruction or spasm of the coronary arteries. People that suffer from average to severe cases of angina have an increased ... [more ▼]

Angina is chest pain induced by ischemia of the heart muscle, generally due to obstruction or spasm of the coronary arteries. People that suffer from average to severe cases of angina have an increased percentage of death before the age of 55, usually around 60%. Therefore, prevention of major complications, optimizing diagnosis, prognosis and therapeutics are of primary importance. The main objective of this study was to uncover biomarkers by comparing serum protein profiles of patients suffering from stable or unstable angina and controls. We identified by non-targeted proteomic approach and confirmed by the means of independent techniques, the differential expression of several proteins indicating significantly increased vascular inflammation response, disturbance in the lipid metabolism and in atherogenic plaques stability. [less ▲]

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See detailOstéomalacie hypophosphatémique hyperphosphaturique avec hypersécrétion de FGF-23
COLSON, Laurent ULg; Vander Rest, Catherine; Reginster, Jean-Yves ULg et al

in Lettre du Rhumatologue (La) (2012), 387

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See detailEvaluation of the cross-reactivity of 25-hydroxyvitamin D2 on seven commercial immunoassays on native samples
LE GOFF, Caroline ULg; PEETERS, Stéphanie ULg; CRINE, Yannick ULg et al

in Clinical Chemistry & Laboratory Medicine (2012), 50(11), 2031-2

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See detailUne infiltration de plasma riche en plaquettes (PRP) améliore les symptômes des tendinopathies patellaires supérieures
Kaux, Jean-François ULg; Croisier, Jean-Louis ULg; Rodriguez de la Cruz, Carlos ULg et al

in 5ème Congrès Commun SFMES & SFTS (2012, October)

Introduction : Le plasma riche en plaquettes (PRP) aurait des effets bénéfiques sur les symptômes des tendinopathies grâce à la libération locale de leurs nombreux facteurs de croissance. Le but de cette ... [more ▼]

Introduction : Le plasma riche en plaquettes (PRP) aurait des effets bénéfiques sur les symptômes des tendinopathies grâce à la libération locale de leurs nombreux facteurs de croissance. Le but de cette étude est d’évaluer les résultats d’une injection de PRP sur la symptomatologie des patients présentant une tendinopathie patellaire supérieure. Matériels et Méthodes : Vingt patients atteints d’une tendinopathie patellaire supérieure, rebelle aux traitements conservateurs classiques depuis au moins 3 mois, ont bénéficié d’une évaluation du statut algo-fonctionnel (EVA, algomètre de pression, VISA-P et IKDC) et des performances physqiues (isocinétique et optojump), ainsi que d’un bilan d’imagerie (échographie et IRM). Ces analyses ont été réalisées en pré-injection ainsi qu’aux 6èmeet 12èmesemaines post-injection. Le test isocinétique évaluait la force des quadriceps et des ischios-jambiers des deux membres suivant différentes modalités (C60°/sec, C240°/sec et E30°/sec). Le test Optojump évaluait la détente et la puissance musculaire par la mesure de la hauteur de saut via deux modalités («Counter Movement Jump » et « Drop Jump »). Le PRP est obtenue à l’aide d’une machine d’aphérèse (COM.TEC, Fresenius-Kabi), permettant d’obtenir une concentration plaquettaire reproductible d’un patient à l’autre, en l’absence quasi totale de globules rouges et blancs. L’infiltration intratendineuse de PRP (6mL) est réalisé à la pointe de la rotule in loco dolenti sans anesthésie locale. Résultats : Les résultats de notre expérimentation démontrent qu’une seule injection locale de PRP accompagnée d’un programme de rééducation excentrique améliore significativement la cicatrisation du tendon patellaire au niveau du score EVA, de l’algomètre de pression, des scores VISA-P et IKDC. Lors du test isocinétique, seuls l’augmentation du MFM des ischios jambiers en C240°/sec, de la différence bilatérale des ischios jambiers en C60°/sec ainsi que de l’EVA en E30°/sec était significative. Les résultats optojump en terme de détente et puissance musculaire ne montrent pas d’amélioration des performances. Néanmoins, l’EVA du « Counter Movement Jump » se voit significativement diminuée après l’infiltration. Enfin, les résultats de l’imagerie (échographie et IRM) ne démontrent pas de modification significative du tendon. Conclusions : L’injection locale de PRP dans le cadre de tendinopathies patellaires supérieure permet une diminution de la symptomatologie douloureuse à 6 semaines, se poursuivant à 12 semaines. La performance fonctionnelle et l’imageries, quant à elles, ne sont pas modifiées. [less ▲]

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See detailLe dosage des acides gras érythrocytaires : comparaison entre une population de référence et des sujets ayant présenté un infarctus aigu du myocarde.
LE GOFF, Caroline ULg; Kaux, Jean-François ULg; Leroy, Ludovic et al

in Immuno-Analyse & Biologie Spécialisée [=IBS] (2012), 27(5), 237-243

The aim of our study was to compare reference values for these FA with data obtained in a population of acute myocardial infarction patients. We performed the quantification of different FA by gas ... [more ▼]

The aim of our study was to compare reference values for these FA with data obtained in a population of acute myocardial infarction patients. We performed the quantification of different FA by gas chromatography associated with flame ionization detector ( FA determination is a new tool we are able to use and to process in our laboratory which can help the clinician to screen patients with the highest cardiovascular risks because of the implication of FA in the etiopathogeny of atherosclerosis. [less ▲]

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See detailPlatelet-rich plasma (PRP) to treat patellar tendinopathy: preliminary results
Kaux, Jean-François ULg; Croisier, Jean-Louis ULg; SIMONI, Paolo ULg et al

in Regional Anesthesia & Pain Medicine (2012, September), 37(5 - Suppl 1), 216

Introduction: Patellar tendinopathy, often remain rebel to conservative treatments. Researches have specifically demonstrated the platelets action as mediator and/or enhancer of tissue healing. The aim of ... [more ▼]

Introduction: Patellar tendinopathy, often remain rebel to conservative treatments. Researches have specifically demonstrated the platelets action as mediator and/or enhancer of tissue healing. The aim of our study was to investigate the effect of 1 injection of PRP in patients suffering from chronic patellar tendinopathy. Methods: Patients performed imaging (US and MRI) and functional assessments, and a clinical examination using an algometer, before treatment and 6 weeks after PRP treatment. They were also invited to answer to questionnaire relative to pain and functional status. PRP was obtained from autologous blood using an apheresis system (COM.TEC, Fresenius). The injection of 6mL of PRP was realised without local anaesthesia into the proximal insertion of the patellar tendon. A 48h rest-time was recommended after infiltration. Afterwards, a submaximal eccentric reeducation was initiated 1 week after infiltration 3 times a week during 5 weeks. In case of pain, anti-inflammatory drugs were prohibited and patient were encouraged to take class I or II painkillers. Results: At this time, 10 patients with patellar tendinopathy were included in our study. Pre-injection tests revealed pain of the upper part of the patellar tendon just below the patella, associated with loss of function. Imaging exams confirmed diagnosis. Six weeks post-injection, the clinical status was improved in all patients, with a significant decrease of algo-functional scores. The pain reported during functional assessments was decreased (in particular for the eccentric actions), yet no significant improvement of physical performances was observed. We found no significant differences between imaging exams before and 6 weeks after PRP injection. Conclusion: One in situ injection of PRP clinically improved patients with patellar tendinopathy 6 weeks after treatment. All the 10 patients reported a decrease of pain during day-life and through physical activities. However nor functional performances neither imaging were improved. [less ▲]

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See detailEffects of platelet-rich plasma (PRP) on the healing of Achilles tendons of rats
Kaux, Jean-François ULg; Drion, Pierre ULg; Colige, Alain ULg et al

in Wound Repair & Regeneration : Official Publication of the Wound Healing Society and the European Tissue Repair Society (2012), 20(5), 748-756

Platelet-Rich Plasma (PRP) contains growth factors involved in the tissular healing process. The aim of the study was to determine if an injection of PRP could improve the healing of sectioned Achilles ... [more ▼]

Platelet-Rich Plasma (PRP) contains growth factors involved in the tissular healing process. The aim of the study was to determine if an injection of PRP could improve the healing of sectioned Achilles tendons of rats. After surgery, rats received an injection of PRP (n=60) or a physiological solution (n=60) in situ. After 5, 15 and 30 days, 20 rats of both groups were euthanized and 15 collected tendons were submitted to a biomechanical test using cryo-jaws before performing transcriptomic analyses. Histological and biochemical analyses were performed on the 5 remaining tendons in each group. Tendons in the PRP group were more resistant to rupture at 15 and 30 days. The mechanical stress was significantly increased in tendons of the PRP group at day 30. Histological analysis showed a precocious deposition of fibrillar collagen at day 5 confirmed by a biochemical measurement. The expression of tenomodulin was significantly higher at day 5. The mRNA level of type III collage, matrix metalloproteinase 2, 3 and 9 was similar in the 2 groups at all time points whereas type I collagen was significantly increased at day 30 in the PRP group. In conclusion, an injection of PRP in sectioned rat Achilles tendon influences the early phase of tendons healing and results in an ultimate stronger mechanical resistance. [less ▲]

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See detailRace cycling: biological evolution
LE GOFF, Caroline ULg; Kaux, Jean-François ULg; Goffaux, Sébastien et al

in Meeusen, R; Duchateau, J; Roelands, B (Eds.) et al Book of Abstracts of the 17th annual Congress of the ECSS (2012, July)

Introduction: The metabolic and cardiac impact of a cycling effort on blood biology is not very well described in the literature. We aimed to measure the concentration of different biomarkers (cardiac and ... [more ▼]

Introduction: The metabolic and cardiac impact of a cycling effort on blood biology is not very well described in the literature. We aimed to measure the concentration of different biomarkers (cardiac and metabolic) released during an international cycling race. Materials and methods: Venous blood samples of 15 young men (25.1 ± 6.4 y.o.) were collected just before (T1), just after (T2), 3 hours (T3) after an international cycling race of 179.6 kilometers in Belgium for the determination of cardiac and metabolic biomarkers: red blood cell (RBC), haemoglobin (HgB), creatinin (Cr), highly sensitive troponin T (hsTnT), myoglobin (MYO) and NT-proBNP. All automated assays were performed according to the manufacter’s specifications. For the statistical analysis, an Anova calculated with the Statistica Software version 9.1 was used. Results and discussions: RBC and HgB levels varied significantly between T0 and T3 (respectively p=0.0026, and p=0.002). Cr concentration also varied significantly between all times (T0-T1:p<0.0001, T1-T3:p=0.0326 and T0-T3 p=0.0001). These changes might be related to renal flow depletion during exercice. MYO increased significantly between T0 and T1 (p<0.0001), but quickly decreased between T1 and T3, however the T3 level stay higher than T0 (p=0.014). The stress delivered from the physical activity performed during the race induced a significant variation of hsTnT which increased significantly between T0 and T1 (p<0.0001) and stayed higher 3 hours after the end of the exercise (T0-T3: p<0.0001). The intense exercise delivery by the race induced a significant variation of NT-proBNP, that followed the same kinetic of hsTnT but in smaller proportion. We noticed variations statistically significant between T0 and T1 and between T0 and T3 for NT-proBNP. These increases of cardiac biomarkers were significant but reasonable and could not allow us to talk about cellular necrosis or irreversible injury. Conclusion: Our results show that stress generated by a cycling race could be the cause for the different metabolic variations observed. Troponin T stays without a doubt the most specific marker for stress related to myocardial tissue. Its increase can then be considered as being of interest. [less ▲]

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See detailImpact of strenous exercise on the release of cardiac biomarkers
LE GOFF, Caroline ULg; MELON, Pierre ULg; Kaux, Jean-François ULg et al

in Meeusen, R; Duchateau, J; Roelands, B (Eds.) et al Book of Abstracts of the 17th annual Congress of the ECSS (2012, July)

Background: Cardiac troponins (cTn) are considered as the best biomarkers for detection of myocardial cell injury and NT-proBNP as the best for the cardiac insufficiency. In this study, cTnT was measured ... [more ▼]

Background: Cardiac troponins (cTn) are considered as the best biomarkers for detection of myocardial cell injury and NT-proBNP as the best for the cardiac insufficiency. In this study, cTnT was measured by new commercially available high-sensitive methods in subjects undergoing the Maasmarathon. Our aim was to compare cTnT and NT-proBNP levels in sportive subjects before and after a strenuous exercise. Materials and Methods: Twenty eight subjects (26 ♂, 42.5±11yo) underwent a race of 42.195 kilometers between Visé (Belgium) and Maastricht (The Netherlands). We drowned blood samples before (T0), just after (T1) and three hours after the race (T3). For all patients, cTnT concentrations were measured by high sensitive methods (hsTnT, Roche Diagnostics) on heparin plasma. The NT-proBNP was also determined with the kit Roche on heparin plasma. The protocol was approved by the ethics committee of the University of Liège (Belgium). All subjects gave their informed consent. All statistical analyses were performed using Medcalc version 8.1 for Windows. p-value <0.01 was regarded as statistically significant. Results and discussion: A significant difference between hsTnT concentrations at T0 and T1 (p<0.0001) was measured as well as between T0 and T3 (p<0.001) for NT-proBNP, but not between T1 and T3. This observation appeared only after a strenuous exercise but today this type of exercise is not reproduce easier in a laboratory of sport. Moreover, at this moment, nobody knows if these observations would have cardiac consequences at long terms. Conclusions: Measurement of cardiac troponins by high sensitive methods allows detecting significant release of biomarkers from the heart during exercise. The levels of NT-proBNP were found significantly increased but in less extent than TnThs. We think that the TnThs could be an interesting marker in the future to help sport medicine to detect risk of developing a cardiac problem. [less ▲]

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See detailInfluence of type of contraction upon tendinous tissue during training: animal model
Kaux, Jean-François ULg; Drion, Pierre ULg; Croisier, Jean-Louis ULg et al

in Meeusen, R; Duchateau, J; Roelands, B (Eds.) et al Book of Abstracts of the 17th annual Congress of the ECSS (2012, July)

Introduction: The treatment of choice for tendinopathies is eccentric reeducation. Although the clinical results appear favourable, the biomechanical changes to the tissue are not yet clear. Materiel and ... [more ▼]

Introduction: The treatment of choice for tendinopathies is eccentric reeducation. Although the clinical results appear favourable, the biomechanical changes to the tissue are not yet clear. Materiel and methods: This study compared the effects of two methods of training (eccentric (E) training and concentric (C) training) with untrained (U) rats. The animals underwent training over a period of five weeks. The tricipital, patellar and Achilles tendons were subsequently removed to perform a traction test to the point of tendon rupture, and a histological analysis was performed. Results: There was a significant improvement in the rupture force of the patellar and tricipital tendons between the U and E groups. The tricipital tendons in the control group presented a significantly smaller cross-section than the E- and C-trained groups. No significant difference was observed for the constraints between the three groups for all three tendons. However, a tendency towards improvement was observed between the trained and the U groups for the patellar tendon. Histological studies demonstrated the development of a greater number of blood vessels and a larger quantity of collagen in the eccentric group. Discussion and conclusion: The mechanical properties of tendons in rats improve after specific training, especially following eccentric training. [less ▲]

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See detailPlatelet-rich plasma (PRP) to treat upper patellar tendinopathies
Kaux, Jean-François ULg; Croisier, Jean-Louis ULg; SIMONI, Paolo ULg et al

in Meeusen, R; Duchateau, J; Roelands, B (Eds.) et al Book of Abstracts of the 17th annual Congress of the ECSS (2012, July)

Introduction: Tendinopathies, especially upper patellar tendinopathy also known as jumper’s knee, often remain rebel to conservative treatments. Several experimental studies have shown the healing ... [more ▼]

Introduction: Tendinopathies, especially upper patellar tendinopathy also known as jumper’s knee, often remain rebel to conservative treatments. Several experimental studies have shown the healing properties of platelet and their growth factors. These factors have the potentiality to improve healing of different tissues: bones, muscles, tendons... Researches have specifically demonstrated the platelets action as mediator and/or enhancer of tissue healing. On the other hand, such treatment has been totally removed of list of doping treatments. The aim of our study was to investigate the effect of 1 injection of PRP in patients suffering from chronic jumper’s knee. Methods: Patients performed imaging (US and MRI) and functional assessments, and a clinical examination using an algometer, before treatment and 6 weeks after PRP treatment. They were also invited to answer to questionnaire relative to pain and functional status. PRP was obtained from autologous blood using an apheresis system (COM.TEC, Fresenius). The injection of 6mL of PRP was realised without local anaesthesia into the proximal insertion of the patellar tendon. A 48h rest-time was recommended after infiltration. Afterwards, a submaximal eccentric reeducation was initiated 1 week after infiltration 3 times a week during 5 weeks. In case of pain, anti-inflammatory drugs were prohibited and patient were encouraged to take class I or II painbrakers. Our protocol was approved by Ethic Committee of University and University Hospital of Liège. Results: Eighteen patients with jumper’s knee were included in our study. Pre-injection tests revealed pain of the upper part of the patellar tendon just below the patella, associated with loss of function. Imaging exams confirmed diagnosis. Six weeks post-injection, the clinical status was improved for the majority of the patients, with a significant decrease of algo-functional scores. The pain reported during functional assessments was decreased (in particular for the eccentric actions), yet no significant improvement of physical performances was observed. We found no significant differences between imaging exams before and 6 weeks after PRP injection. Conclusion: One in situ injection of PRP clinically improved patients with jumper’s knee 6 weeks after treatment. Most patients reported a decrease of pain during day-life and through physical activities. However nor functional performances neither imaging were improved. A followup at 3 months is actually in process to evaluate more long term efficacy of PRP treatment. [less ▲]

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See detailCross-reactivity of 25-hydroxyvitamin D2 in ADVIA Centaur Total Vitamin D
FORTUNATO, Antonio; LE GOFF, Caroline ULg; PEETERS, Stéphanie ULg et al

Poster (2012, May)

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See detailEffects of platelet-rich plasma on the healing of tendons: animal model
Kaux, Jean-François ULg; Drion, Pierre ULg; Colige, Alain ULg et al

in Biomedica 2012 (2012, April)

Introduction: Platelet-Rich Plasma (PRP) contains lot of growth factors which could enhance the healing process of different tissues. We aimed to determine if a single injection of PRP could improve the ... [more ▼]

Introduction: Platelet-Rich Plasma (PRP) contains lot of growth factors which could enhance the healing process of different tissues. We aimed to determine if a single injection of PRP could improve the cicatrisation of ruptured Achilles tendons of rats. Material and Methods: A 5mm defect was surgically made in the Achilles tendon of 120 rats. A few hours after surgery, 45 rats received a PRP or PBS injection in situ. After 5, 15 and 30 days, 20 rats of both groups were euthanized and 15 collected tendons were immediately submitted to a biomechanical tensile strength test until rupture using a “cryo-jaw” device. After, theses samples were used for transcriptomic analyses. Histological and biochemical analyses were performed on the five remained tendons in each group. Results: Tendons in the PRP group were more resistant to rupture at 15 and 30 days than those in the control group. The transverse area of tendons in the PRP group was significantly higher at day 5 and 15. The constraint was significantly increased in tendons of the PRP group in the late phase of the healing (day 30). Histological and immunohistological analysis showed an increased staining for fibrillar collagen at day 5 confirmed by a biochemical analysis showing an increased collagen concentration in the callus. The expression of tenomodulin, a tenocyte differentiation marker, was significantly higher in the PRP-treated tendons at day 5. No significant difference in terms of mRNA for type III collagen and matrix metalloproteinase 9 was observed at any time between the 2 groups. Conclusion: A single injection of PRP in sectioned Achilles tendon of rats few hours after surgery influences the early phase of tendons healing, resulting in an ultimate stronger mechanical resistance. [less ▲]

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See detailPlatelet-rich plasma to treat jumper’s knee: preliminary results
Kaux, Jean-François ULg; CROISIER, Jean-Louis ULg; SIMONI, Paolo ULg et al

in Biomedica 2012 (2012, April)

Introduction: Tendinopathies, especially jumper’s knee, often remain rebel to conservative treatments. Several experimental studies have shown the healing properties of platelet and their growth factors ... [more ▼]

Introduction: Tendinopathies, especially jumper’s knee, often remain rebel to conservative treatments. Several experimental studies have shown the healing properties of platelet and their growth factors. Researches have specifically demonstrated the platelets action as mediator and/or enhancer of tissue healing. The aim of our study was to investigate the effect of 1 injection of PRP in patients suffering from chronic jumper’s knee. Our protocol was approved by Ethic Committee of University and University Hospital of Liège. Methods: Patients performed imaging (US and MRI) and functional assessments, and a clinical examination using an algometer, before treatment and 6 weeks after PRP treatment. They were also invited to answer to questionnaire relative to pain and functional status. PRP was obtained from autologous blood using an apheresis system (COM.TEC, Fresenius). The injection of 6mL of PRP was realised without local anaesthesia into the proximal insertion of the patellar tendon. A 48h rest-time was recommended after infiltration. Afterwards, a submaximal eccentric reeducation was initiated 1 week after infiltration 3 times a week during 5 weeks. In case of pain, anti-inflammatory drugs were prohibited and patient were encouraged to take class I or II painbrakers. Results: At this time, 10 patients with jumper’s knee were included in our study. Pre-injection tests revealed pain of the upper part of the patellar tendon just below the patella, associated with loss of function. Imaging exams confirmed diagnosis. Six weeks post-injection, the clinical status was improved in all patients, with a significant decrease of algo-functional scores. The pain reported during functional assessments was decreased (in particular for the eccentric actions), yet no significant improvement of physical performances was observed. We found no significant differences between imaging exams before and 6 weeks after PRP injection. Conclusion: One in situ injection of PRP clinically improved patients with jumper’s knee 6 weeks after treatment. All the 10 patients reported a decrease of pain during day-life and through physical activities. However nor functional performances neither imaging were improved. [less ▲]

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See detailBiological variations during a race cycling
LE GOFF, Caroline ULg; Kaux, Jean-François ULg; Goffaux, Sébastien et al

in Biomedica 2012 (2012, April)

Background: The metabolic and cardiac impact of a cycling effort on blood biology is not very well described in the literature. We aimed to measure the concentration of different biomarkers (cardiac and ... [more ▼]

Background: The metabolic and cardiac impact of a cycling effort on blood biology is not very well described in the literature. We aimed to measure the concentration of different biomarkers (cardiac and metabolic) released during an international cycling race. Materials and Methods: Venous blood samples of 15 young men (25.1 ± 6.4 y.o.) were collected just before (T1), just after (T2), 3 hours (T3) after an international cycling race of 176 kilometers in Belgium for the determination of cardiac and metabolic biomarkers: red blood cells (RBC) haemoglobin (HgB) creatinin (Cr) highly sensitive troponin T (hsTnT) myoglobin (MYO) NT-proBNP All automated assays were performed according to the manufacter’s specifications. For the statistical analysis, an Anova calculated with the Statistica Software version 9.1 was used. Results: •RBC and HgB levels varied significantly between T0 and T3 (respectively p=0.0026, and p=0.002) (Fig. 1 and 2). • Cr concentration also varied significantly between all times (T0-T1:p<0.0001, T1-T3:p=0.0326 and T0-T3 p=0.0001)(Fig.3). These changes might be related to renal flow depletion during exercise. •MYO increased significantly between T0 and T1 (p<0.0001), but quickly decreased between T1 and T3, however the T3 level stay higher than T0 (p=0.014) (Fig.4). •The stress delivered from the physical activity performed during the race induced a significant variation of hsTnT which increased significantly between T0 and T1 (p<0.0001) and stayed higher 3 hours after the end of the exercise (T0-T3: p<0.0001) (Fig.5) . •The intense exercise delivery by the race induced a significant variation of NT-proBNP, that followed the same kinetic of hsTnT but in smaller proportion. We noticed variations statistically significant between T0 and T1 and between T0 and T3 for NT-proBNP (Fig.6). •These increases of cardiac biomarkers were significant but reasonable and could not allow us to talk about cellular necrosis or irreversible injury. Conclusions: Our results show that stress generated by a cycling race could be the cause for the different metabolic variations observed. Troponin T stays without a doubt the most specific marker for stress related to myocardial tissue. Its increase can then be considered as being of interest. [less ▲]

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See detailLe dosage des acides gras érythrocytaires : comparaison entre une population de référence et des sujets ayant présenté un infarctus aigu du myocarde
LE GOFF, Caroline ULg; Leroy, Ludovic; Kaux, Jean-François ULg et al

Poster (2012, February)

Introduction Un acide gras (AG) est un acide carboxylique avec une longue chaine aliphatique, qui peut être saturé ou insaturé. Récemment, le rôle des AG, particulièrement, celui des oméga-3 et oméga-6, a ... [more ▼]

Introduction Un acide gras (AG) est un acide carboxylique avec une longue chaine aliphatique, qui peut être saturé ou insaturé. Récemment, le rôle des AG, particulièrement, celui des oméga-3 et oméga-6, a émergé comme facteur de risque cardiovasculaire dans la littérature. Le but de ce travail était de comparer les taux d’AG mesurés dans une population saine avec ceux obtenus chez des patients admis aux urgences pour un infarctus du myocarde. Matériels et méthodes Cinquante quatre sujets sains (33±11ans, 31 femmes) ont été sélectionnés comme population de référence. Ces derniers ont été sélectionnés selon leur mode de vie (alimentation, tabac, etc). En parallèle, nous avons dosé les AG chez 33 patients (55±9 ans, 12 femmes) admis dans le service d’urgence de notre institution pour infarctus aigu du myocarde (AMI). Le sang était prélevé sur EDTA. Avant l’analyse, les échantillons étaient lavés et transméthylés. Le dosage des AG était réalisé par chromatographie gazeuse couplée à un détecteur à ionisation de flamme (GCFID). Après le dosage, nous avons réalisés une comparaison des taux, sommes et rapport entre les différents AG ainsi que l’index oméga-3 (somme de l’acide eicosapentaénoïque et docosahexaénoïque) obtenus dans les 2 groupes. Résultats. Des valeurs de référence ont été obtenues pour notre population de référence via le logiciel de statistique MedCalc. Dans le groupe AMI, les taux d’oméga-6 étaient significativement plus élevés (p<0.05) pour C18:2n6 (Figure 1) et C18:3n6 (Figure 2)comparés aux résultats obtenus dans la population de référence. Par contre les taux d’oméga-3 étaient significativement plus bas (p<0.01) par rapport aux sujets de référence pour le C22 :6n3 (Figure 3). L’index oméga-3 montrait une valeur plus basse et le rapport oméga-6/oméga-3 était plus élevé dans le groupe AMI comparé aux sujets de référence. Conclusion Le dosage des AG est un nouvel outil que le laboratoire peut proposer aux cliniciens afin de stratifier les patients présentant le plus de risque cardiovasculaire avant ou après un infarctus du myocarde. Ces patients pourraient ainsi être supplémenté en acides gras oméga-3 afin d’éviter les récidives d’infarctus du myocarde ou de diminuer la formation de la plaque d’athérosclérose avant le premier accident. [less ▲]

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See detailApport des troponines T et I ultrasensibles dans le diabète
LE GOFF, Caroline ULg; LAURENT, Terry ULg; GARWEG, Christophe ULg et al

in Immuno-Analyse & Biologie Spécialisée [=IBS] (2012, February), 27(1), 40

Introduction : Le diabète atteint environ 3% de la population française. Or, cette maladie expose à l'apparition précoce de complications cardiovasculaires. Les troponines de nouvelles générations ... [more ▼]

Introduction : Le diabète atteint environ 3% de la population française. Or, cette maladie expose à l'apparition précoce de complications cardiovasculaires. Les troponines de nouvelles générations hautement sensibles pourraient être un bon outil diagnostic pour mettre en évidence des macroou micro-angiopathies non diagnostiquée chez ces patients. Le but de notre étude était de comparer la performance de la troponine T ultrasensible (hsTnT) (Roche Diagnostic) avec la troponine I ultrasensible (TnI II) (Abbott Diagnostic) chez le sujet diabétique. Matériel et méthodes : Vingt patients diabétiques (âge moyen : 52.6 ± 8.4 ans) ont été sélectionnés sur le critère d’un taux d’hémoglobine glyquée (HbA1c) élevé. Ces sujets ont été comparés à vingt sujets contrôles d’âge moyen : 60.05 ± 2.86 ans. Les patients atteints d’insuffisance rénale et d’affections cardiovasculaires ont été exclus. Les analyses ont été réalisées sur du plasma hépariné lithium. La hsTnT a été dosée par électrochemiluminescence sur le Modular E (Roche Diagnostic). Abbott utilise des microparticules chemiluminescentes pour le dosage de la TnI II sur l’ARCHITECT i. Résultats : Les sujets diabétiques ont un taux plasmatique de hsTnT représenté par les valeurs suivantes [médiane (1er quartile, 3ème quartile)] : 0,007 (0,03 ; 0,018) ng/mL ; alors que les sujets contrôles négatifs présentent les valeurs suivantes : 0,003 (0,003 ; 0,004) ng/mL. La différence de taux de hsTnT observée entre ces 2 populations est statistiquement significative (p = 0,000922). Pour la TnI II, on observe un taux plasmatique de: 0,004 (0,003 ; 0,075) ng/mL chez les sujets diabétiques et 0,002 (0,001 ; 0,005) ng/mL chez les sujets contrôles. La différence de taux de TnI II observée entre ces 2 populations n’est pas statistiquement significative (p > 0,005). Discussion-conclusion : Au décours d’un diabète, il apparait que les taux de hsTnT sont augmentés de façon significative par rapport au groupe des contrôles négatifs. Si les taux hsTnT augmentent chez le diabétique, il semblerait normal qu’il en soit de même pour la TnI II, vu que ces deux marqueurs sont intimement liés. Nous ne l’avons pas observé mais ceci peut être du à la plus faible performance analytique du dosage de la TnI II utilisé. Cette libération de hsTnT dans le sang peut être due aux micro- et macro-angiopathies au niveau des coronaires induites au cours d’un diabète. [less ▲]

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