References of "Jijakli, Haissam"
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See detailPre-harvest conditions affect the banana susceptibility to post-hervest disease.
Lassois, Ludivine ULg; Ewane, Cecile; Forret, Marie et al

Conference (2011, May 24)

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See detailIn situ proteome study of Pichia anomala strain K, an antagonist of the apple pathogen Botrytis cinerea
Kwasiborski, Anthony ULg; Renaut, Jenny; Delaplace, Pierre ULg et al

Conference (2011, May 24)

Postharvest fungal pathogens, mainly Botrytis cinerea, Penicillum expansum and Gloeosporium spp., annually cause 5-20% losses of fruit. Control measures against these diseases include chemical fungicide ... [more ▼]

Postharvest fungal pathogens, mainly Botrytis cinerea, Penicillum expansum and Gloeosporium spp., annually cause 5-20% losses of fruit. Control measures against these diseases include chemical fungicide applications, but the development of resistant fungal explains the growing interest for biological control. Pichia anomala strain K was previously identified as an efficient antagonist of pathogens on apples. Indeed, the percentage of protection of P.anomala against B.cinerea reached from 90 to 100% on apple wounds according to the experimental conditions. Microbiological, biochemical and molecular approaches demonstrated the implication of exo-β-1,3-glucanases in the mechanism of action of P.anomala. However, study of these mechanisms could be improved by observations under natural infection conditions in order to take into account the tripartite interactions host/antagonist/pathogen. The proteomic tool allowed an overview of process implicated in the antagonism against B.cinerea in such conditions. One 50mm wound per apple were covered by a membrane and inoculated by a P.anomala suspension then by B.cinerea or not. Samples were collected during the exponential and stationary phase to identify the early and later responses to the presence of B.cinerea. After extraction, proteins were separated on 2DE gels. Spots influenced by the presence of B.cinerea in exponential and stationary phases were identified by MALDI-ToF. One hundred five and sixty spots of proteins were influenced by the presence of B.cinerea in exponential and stationary phase respectively. In exponential phase, influenced proteins were implicated in the different steps of the proteins biosynthesis: amino acid synthesis, translation or mRNA maturation and in energy synthesis. On the other hand, in stationary phase, influenced proteins were mainly implicated in energy metabolic pathway: glycolysis, alcoholic fermentation or gluconeogenesis. The presence of B.cinerea seemed to induce a slowdown in the metabolism of P.anomala without influenced its growth. Further studies have to be realized to understand the complexity of these modes of action. [less ▲]

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See detailScreening Elisa method to assess in vitro efficacy of new agents based on Lactoperoxydase system against phytopathogens
Bafort, Françoise ULg; Lepoivre, Philippe ULg; Jijakli, Haissam ULg et al

Poster (2011, May 24)

The lactoperoxydasesystem (LPS) is a natural biological protection system in human saliva, milk and tears. Lactoperoxydase catalyzes the oxidation of iodide (I-) and thiocyanate (SCN-) in presence of ... [more ▼]

The lactoperoxydasesystem (LPS) is a natural biological protection system in human saliva, milk and tears. Lactoperoxydase catalyzes the oxidation of iodide (I-) and thiocyanate (SCN-) in presence of hydrogen peroxide in hypoiodite (OI-) and hypothiocyanite (OSCN-) ions. Both ions have inhibitory action against pathogenic bacteria, fungi and viruses. Until now, the ions OI- and OSCN- applied separately from the LPS were not tested against plant pathogens. Seventeen KI/KSCN/H2O2 ratios with 3 dilutions for each one were tested against Botrytiscinerea. Results showed that the ions OI- and OSCN- gives a better % of inhibition with: -high concentration of KI+KSCN, -a ratio of KI/KSCN of 4.5 and -a ratioof (KI+KSCN)/H2O2 near 1. Increased dilution combined with lower KI+KSCN concentration reduced the efficacy of the ions. From the screening against B.cinerea, 4 ratios of KI+KSCN were selected and tested against 6 other phytopathogens. Results showed that the OI- and OSCN- ions give 87 and 100% inhibition against Penicillium expansum and Verticillium dahlia respectively. The inhibitory activity of the ions solution was ranged between 70 and 79% regarding Fusarium avenaceum, Phytophthora infestans and Colletotrichum lindemuthanium. The ions OI- and OSCN- showed an insufficient inhibitory activity against Septoria tritici. The screening method highlighted that high KI/KSCN ratios and low dilution were more efficient against phytopathogens. The efficient ratios will be further evaluated in vivo. [less ▲]

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See detailProduction and oil-emulsion formulation of Cadophora malorum and Alternaria jacinthicola, two biocontrol agents against Water Hyacinth (Eichhornia crassipes)
Dagno, Karim ULg; Lahlali, Rachid; Diourté, Mamourou et al

in African Journal of Microbiology Research [=AJMR] (2011), 5(8), 924-929

Cadophora malorum isolate Mln715 and Alternaria jacinthicola strain MUCL 53159 are under <br />development as biocontrol agents against Water Hyacinth (Eichhornia crassipes) in Mali. Production of spores ... [more ▼]

Cadophora malorum isolate Mln715 and Alternaria jacinthicola strain MUCL 53159 are under <br />development as biocontrol agents against Water Hyacinth (Eichhornia crassipes) in Mali. Production of spores of these agents on locally available substrates (Water Hyacinth, powdered paddy rice chaff, wheat semolina) was assessed with a view to mass production. The C. malorum isolate sporulated best on Water Hyacinth (4.08 × 107 spores ml-1), followed by wheat (1.06 ×107 spores ml-1), whereas A. jacinthicola produced more spores on paddy rice chaff and wheat (0.24 x 107 spores ml-1). The severity of the damage caused by each pathogen was evaluated in the greenhouse and in the field. Under both greenhouse and field conditions, the biocontrol efficacy of the fungal isolates was improved with (unrefined) Carapa procera (L.) oil or (refined) palm oil, supplemented with soybean lecithin and Tween 20. When such a formulation was used, the incubation time was 4 to 5 days in the greenhouse and 7 to 9 days on the field, and the damage severity (DS) recorded 6 weeks after treatment varied from 87.02 to 93.13% in the greenhouse and from 59.11 to 63.00% in the field. For unformulated C. malorum and A. jacinthicola respectively, the incubation times were longer and the DS values were only 22.11 and 29.05% in the greenhouse and 12.05 and 15.15% on the field. Our results highlight good substrates for mass production of these mycoherbicides and demonstrate the ability of vegetable oil formulations to improve their efficacy. [less ▲]

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See detailRôle des acides aminés dans l'activité antagoniste d'Aureobasidium Pullulans vis-à-vis de la pourriture bleue des pommes en post-récolte
Krimi Bencheqroun, Sanae; Bajji, Mohammed; Laghilili, Mustapha et al

Conference (2011, March 23)

Aureobasidium pullulans (souche Ach1-1) a montré une grande efficacité vis-à-vis de Penicillium expansum agent de la pourriture bleue des pommes en conservation. Le présent travail a été réalisé dans le ... [more ▼]

Aureobasidium pullulans (souche Ach1-1) a montré une grande efficacité vis-à-vis de Penicillium expansum agent de la pourriture bleue des pommes en conservation. Le présent travail a été réalisé dans le cadre de l’analyse des mécanismes d’action qui sont les plus impliqués dans son activité antagoniste. Dans des essais in vivo sur pommes blessées, l’effet protecteur de la souche Ach1-1 vis-à-vis de P. expansum a été significativement affaibli par l’ajout dans les blessures de concentrations élevées en acides aminés. L’importance de la réduction étant dépendante de la concentration appliquée, a montré que la compétition pour les acides aminés joue un rôle important dans la suppression de P. expansum. Ce résultat a été appuyé par l’analyse biochimique de la cinétique de l’épuisement des acides aminés dans les blessures des pommes qui a dévoilé que ces composés et particulièrement la sérine, la glycine et l’acide glutamique sont mieux métabolisés par la souche antagoniste que par le pathogène. L’ajout en excès de ces trois acides aminés individuellement dans les blessures des pommes a fortement réduit l’efficacité de la souche Ach1-1 vis-à-vis de P. expansum. Ainsi, ces acides aminés semble être parmi les éléments les plus limitants dans le mécanisme de la compétition. Ces résultats indiquent que l’antagoniste A. pullulans souche Ach 1-1 exerce une activité fongistatique plus que fongicide vis-à-vis de P. expansum et agit par une compétition efficace pour les éléments nutritifs limitants des blessures des pommes et particulièrement les acides aminés sans affecter la viabilité du pathogène. [less ▲]

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See detailControl of apple blue mold by the antagonistic yeast pichia anomala strain K: screening of UV protectants for preharvest application
Lahlali, Rachid; Brostaux, Yves; Jijakli, Haissam ULg

in Plant Disease (2011), 95(3), 311-316

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See detailEffect of temperature and water activity on spore germination and mycelial growth of three fungal biocontrol agents against water hyacinth (Eichhornia crassipes)
Dagno, Karim ULg; Lahlali, Rachid; Diourté, M. et al

in Journal of Applied Microbiology (2011), 110

The fungi were grown in vitro on plates containing potato dextrose agar medium at different aw values (glycerol being added to adjust the aw). The percentage of viable conidia and radial growth rate ... [more ▼]

The fungi were grown in vitro on plates containing potato dextrose agar medium at different aw values (glycerol being added to adjust the aw). The percentage of viable conidia and radial growth rate decreased with decreasing water activity. Statistical analysis showed a significant effect of aw, temperature and the aw temperature interaction on mycelial growth (P < 0Æ0001). Water activity emerged as the factor exerting the greatest influence. Differences were observed between the fungi tested, the C. malorum appearing more tolerant to low aw and the F. sacchari more tolerant to high temperature (35 C). Growth models predicting the combined effect of aw and temperature were developed and response surfaces generated, showing fairly good agreement with the experimental values. Conclusions: Our results confirm the previous finding that aw has a greater influence than temperature on fungal growth. Under most conditions, variation of environmental factors has a detrimental influence on the percentage of viable conidia and mycelial growth rate of fungal isolates. [less ▲]

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See detailDevelopment of a Quick Quantitative Real-Time PCR for the In Vivo Detection and Quantification of Peach latent mosaic viroid
Parisi, Olivier ULg; Lepoivre, Philippe ULg; Jijakli, Haissam ULg

in Plant Disease (2011), 95(2), 137-142

Viroids are plant pathogens infecting a broad range of herbaceous and tree crops. Among them, the Peach latent mosaic viroid (PLMVd) infects mainly peach trees, causing a loss of production with no ... [more ▼]

Viroids are plant pathogens infecting a broad range of herbaceous and tree crops. Among them, the Peach latent mosaic viroid (PLMVd) infects mainly peach trees, causing a loss of production with no curative options. Detecting this viroid is thus important for certification procedures aiming to avoid the release of infected material into orchards. Presented here is a complete detection method based on reverse transcription (RT) followed by a quantitative real-time polymerase chain reaction (PCR). New primers were selected and optimal reaction conditions determined for routine application of the method. The technique is 105 times more sensitive than the endpoint RT-PCR used for PLMVd detection, and permits earlier detection of PLMVd in infected plants. The quick, low-cost extraction procedure used and the quality of the results obtained make this method suitable for routine testing. [less ▲]

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See detailCatecholamine biosynthesis pathway potentially involved in banana defense mechanisms to crown rot disease
Lassois, Ludivine ULg; De Clerck, Caroline ULg; Frettinger, Patrick et al

in Communications in Agricultural and Applied Biological Sciences (2011), 76(4), 591-601

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See detailPredictive modelling of the combined effect of temperature and water activity (aw) on the in vitro growth of Erwinia spp infecting potato tubers in Belgium
Moh, Augustin; Massart, Sébastien ULg; Lahlali, Rachid et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2011), 15(3), 378-386

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See detailApple stem grooving virus
Massart, Sébastien ULg; Jijakli, Haissam ULg; Kummert, Jean

in Hadidi, A.; Barba, M.; Candresse, T. (Eds.) et al Virus and Virus-Like Diseases of Pome and Stone Fruits (2011)

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See detailUV protectants for Candida Oleophila (strain O), a biocontrol agent of post-harvest fruit disease
Lahlali, R.; Buonatesta, R.; Jijakli, Haissam ULg

in Plant Pathology (2011), 60(2), 288-295

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See detailEfficacy assessment of Pichia guilliermondii strain Z1, a new biocontrol agent, against citrus blue mould in Morocco under the influence of temperature and relative humidity
Lahlali, Rachid; Hamadi, Younes; El Guilli, Mohammed et al

in Biocontrol Science (2011), 56

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See detailEffects of Temperature and Relative Humidity on the In Vitro and In Vivo Radial Growth of Penicillium italicum and on the Biocontrol Activity of Pichia guilliermondii, Strain Z1
El Guilli, M.; Ibriz, M.; Lahlali, Rachid et al

in Acta Horticulturae (2011), 905

The objective of this study was to assess the effect of temperature (5-25°C) on the ‘in vitro’ and ‘in vivo’ growth rates of Penicillium italicum and to determine the combined effect of temperature and ... [more ▼]

The objective of this study was to assess the effect of temperature (5-25°C) on the ‘in vitro’ and ‘in vivo’ growth rates of Penicillium italicum and to determine the combined effect of temperature and relative humidity (45 to 100%) on lesion size of this pathogenic fungus on Valencia late oranges, either alone or in combination with the antagonistic yeast strain Z1 of Pichia guilliermondii Wickerham. Statistical analysis showed a significant effect of temperature on the ‘in vitro’ and ‘in vivo’ radial growth of P. italicum with the maximum growth observed at temperature of 25°C. In both cases, no growth was observed at a temperature of 35°C. These factors had a significant effect on P. italicum lesion size when it was applied alone on Valencia late oranges and insignificant when yeast strain Z1 was applied 24 h before P. italicum inoculation. Our results confirm previous ‘in vitro’ findings that aw has a greater influence than temperature on P. italicum growth and highlight that the strain Z1 showed high antagonistic potential against this pathogen over a range of temperature-relative humidity regimes favouring P. italicum development. [less ▲]

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See detailPichia anomala in biocontrol for apples: 20 years of fundamental research and practical applications
Jijakli, Haissam ULg

in Antonie van Leeuwenhoek (2011), 99(1), 93-105

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See detailIdentification of genes involved in the response of banana to crown rot disease
Lassois, Ludivine ULg; Frettinger, Patrick; de Lapeyre de Bellaire, Luc et al

in Molecular Plant-Microbe Interactions [=MPMI] (2011), 24(1), 143-153

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See detailNovel applications of lactoperoxydase system against pathogens of cultivated plants
Jijakli, Haissam ULg; Bafort, Françoise ULg; perraudin, J.P

Conference (2010, June)

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