References of "Jijakli, Haissam"
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See detailThe Lactoperoxydase System, a soft chemical method to control of potato late blight
Bafort, Françoise ULg; Jijakli, Haissam ULg; Perraudin, Jean-Paul

Poster (2012, May 07)

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See detailModels to predict the combined effects of temperature and relative humidity on Pectobacterium Atrosepticum and Pectobacterium carotovorum subsp. Carotovorum population density and soft rot disease development at the surface of wounded potato tubers
Moh, Augustin; Massart, Sébastien ULg; Lepoivre, Philippe ULg et al

in Journal of Plant Pathology [=JPP] (2012), 94(1), 181-191

The main objectives of this study were to evaluate and model the influence of temperature (10, 15 and 20°C), relative humidity (86, 96 and 100%) and initial concentration of bacterial inoculum (105, 107 ... [more ▼]

The main objectives of this study were to evaluate and model the influence of temperature (10, 15 and 20°C), relative humidity (86, 96 and 100%) and initial concentration of bacterial inoculum (105, 107 et 109 CFU ml-1) on the population density of Pectobacterium atrosepticum (Pba) and Pectobacterium carotovorum subsp. carotovorum (Pcc) which are important potato pathogens in temperate climates, and on the development of soft rot symptoms caused by these bacteria at the surface of wounded potatoes tubers under controlled conditions. Experiments were carried out according to a Box-Behnken experimental design, simplifying prediction of the combined effects of three controlled factors. With both bacterial species, statistical analysis showed a significant effect of temperature, relative humidity and initially applied bacterial concentration on population dynamics and soft rot development at the surface of wounded potato tubers. Multiple regression analyses and the contour plots showed that the temperature is the most important factor, followed by the initially applied bacteria concentration and relative humidity. More than 64% of the variability of the soft rot symptoms observed could be explained by the presence of Pba and Pcc at the level of wounded potato tubers under the combined effect of tested factors. The quadratic polynomial models developed in our research should integrate the heterogeneity of tested bacteria belonging to the same species (which was not evaluated in this preliminary investigation) in further research. [less ▲]

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See detailEffect of a fungal infection on the profile of volatile organic compounds emitted by plant roots
Fiers, Marie ULg; Lognay, Georges ULg; Wathelet, Jean-Paul ULg et al

in Communications in Agricultural and Applied Biological Sciences (2012), 77(3), 125-129

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See detailBiocontrol and other alternative control methods against pre-and post-harvest pome fruit diseases.
Jijakli, Haissam ULg

in Creemers, Piet (Ed.) Intergrated Plant Protection in Fruit Crops. Subgroup"Pome Fruit Diseases" (2012)

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See detailMicroscale ELISA plate screening of essential oils against European damageable plant pathogens
Parisi, Olivier ULg; Zhiri, Abdesselam; Baudoux, Dominique et al

Conference (2012)

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See detailDevelopment and application of a microplate method to evaluate the efficacy of essential oils against Penicillium italicum Wehmer, Penicillium digitatum Sacc. and Colletotrichum musea (Berk. M.A. Curtis) Arx, three postharvest fungal pathogens of fruits
Kouassi, Kouadio Hugues Sosthène ULg; Bajji, M.; Brostaux, Yves ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2012), 16(3), 325-336

A microbioassay was developed for evaluating the in vitro antifungal activity of 30 preselected essential oils. A template based on 10 serial dilutions with eight replicates per dilution arranged on two ... [more ▼]

A microbioassay was developed for evaluating the in vitro antifungal activity of 30 preselected essential oils. A template based on 10 serial dilutions with eight replicates per dilution arranged on two 96-well ELISA plates was used as a reproducible and standardized design to identify the in vitro effectiveness of these essential oils against Penicillium italicum Wehmer, Penicillium digitatum Sacc. and Colletotrichum musea (Berk. M.A. Curtis) Arx, three postharvest fungal pathogens, on fruits. Growth of mycelium was monitored by measuring optical density (492 nm). Cinnamomum zeylanicum, Cinnamomum verum and Eugenia caryophyllus were found to be still active against all the three pathogens even at 100 ppm. Compared to other methods, this microbioassay proved to be a rapid, reproducible, and efficient method for testing the efficacy of essential oils that inhibit spore germination in P. italicum, P. digitatum and C. musea. The assay requires relatively small amounts of essential oils. [less ▲]

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See detailThe control of postharvest blue and green molds of citrus in relation with essential oil-wax formulations, adherence and viscosity.
Kouassi, Kouadio Hugues Sosthène ULg; Bajji, Mohammed; Jijakli, Haissam ULg

in Postharvest Biology & Technology (2012), 73

The use of wax coatings enriched with antifungals has significantly contributed to quality maintaining of harvested citrus fruit. On the other hand, interest in essential oils (EOs) as an alternative to ... [more ▼]

The use of wax coatings enriched with antifungals has significantly contributed to quality maintaining of harvested citrus fruit. On the other hand, interest in essential oils (EOs) as an alternative to synthetic fungicides has recently gained momentum. In this study, Cinnamomum zeylanicum EO was incorporated into a variety of commercial citrus waxes (shellac, carnauba, paraffin and polyethylene). The biological activity of these formulations against green and blue rots as well as their viscosity and adherence to the orange fruit surface were evaluated. Excellent disease control was achieved with C. zeylanicum EO incorporated in shellac and/or carnauba wax compared to other EO–wax formulations. Disease control by EO–waxes seems to depend not only on the volume that remains on the fruit skin, but also, probably on the retention of EO components on the fruit. Other factors such as formulation solubility, permeability to gases, and compatibility between EO compounds and those of waxes may also be involved in the improvement of EO efficacy. The present study may therefore allow a careful selection of ppropriate waxes for the elaboration of effective EO–wax formulations. [less ▲]

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See detailPresent status of the development of mycoherbicides against water hyacinth: successes and challenges. A review
Dagno, Karim; Lahlali, Rachid; Diourté, Mamourou et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2012), 16(3), 360-368

Recent trends in the implementation of bioherbicide use in the control of water hyacinth (Eichhornia crassipes [Martius] Solms Laubach) have depended primarily on several strategies. The use of ... [more ▼]

Recent trends in the implementation of bioherbicide use in the control of water hyacinth (Eichhornia crassipes [Martius] Solms Laubach) have depended primarily on several strategies. The use of bioherbicides has been stimulated as part of the search for alternatives to chemical control, as the use of these more environmentally-friendly formulations minimizes hazards resulting from herbicide residue to both human and animal health, and to the ecology. In addition, one of the major strategies in the concept of biological control is the attempt to incorporate biological weed control methods as a component of integrated weed management, in order to achieve satisfactory results while reducing herbicide application to a minimum. Several fungal pathogens with mycoherbicide potential (Sclerotinia sclerotiorum in Hyakill™ and Cercospora rodmanii, named ABG-5003) have been discovered on diseased water hyacinth plants, but none has become commercially available in the market. Biological, technological, and commercial constraints have hindered progress in this area. Many of these constraints are being addressed, but there is a critical need to better understand the biochemical and physiological data regarding the pathogenesis of these new bioherbicides. Oil emulsions are recognized as a way to increase both efficiency of application and efficacy of biocontrol agents. [less ▲]

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See detailFungi occurring on waterhyacinth (Eichhornia crassipes (Martius) Solms-Laubach) in Niger River in Mali and their evaluation as mycoherbicides.
Dagno, Karim; Lahlali, Rachid; Diourté, Mamourou et al

in Journal of Aquatic Plant Management (2012), 50

We recovered 116 fungal isolates in 7 genera from waterhyacinth plants having pronounced blight symptoms collected in Mali. Isolation frequency of the genera was Curvularia (60.32%), Fusarium (42.92 ... [more ▼]

We recovered 116 fungal isolates in 7 genera from waterhyacinth plants having pronounced blight symptoms collected in Mali. Isolation frequency of the genera was Curvularia (60.32%), Fusarium (42.92%), Alternaria (11.6%), Coniothyrium (11.6%), Phoma (3.48%), Stemphylium (3.48%), and Cadophora (1.16%). On the basis of in vivo pathogenicity tests in which the diseased leaf area percentage and disease severity were visually estimated using a disease severity index, three isolates, Fusarium sp. Mln799, Cadophora sp. Mln715, and Alternaria sp. Mlb684 caused severe disease. These were later identified as Gibberella sacchari Summerell & J.F. Leslie, Cadophora malorum (Kidd & Beaumont) W. Grams, and Alternaria sp. respectively. This is the first report to highlight C. malorum as a candidate biocontrol agent against waterhyacinth. Neither C. malorum Mln715 nor Alternaria sp. Mlb684 in host specificity tests showed any pathogenicity toward 17 crop plants of economic importance in Mali. [less ▲]

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See detailCharacterization of a novel chitinase from a moderately halophilic bacterium, Virgibacillus marismortui strain M3-23
Essghaier, Badiaa; Hedi, Abdeljabbar; Bajji, Mohammed et al

in Annals of Microbiology (2012), 62(2), 835-841

A new chitinase produced by the moderately halophilic bacterium Virgibacillus marismortui strain M3- 23 was identified and characterized. Distinguishable characteristics of high activity and stability at ... [more ▼]

A new chitinase produced by the moderately halophilic bacterium Virgibacillus marismortui strain M3- 23 was identified and characterized. Distinguishable characteristics of high activity and stability at different pH, temperatures and salinity of M3-23 chitinase are reported. Analysis of the catalytic domain sequence from the enzyme highlighted its relationship to glycosyl hydrolase family 18. Comparison of the deduced chitinase sequence from strain M3-23 to known chitinases from Bacillus species showed low similarity (82%), suggested its novelty. This is the first report of the characterization of chitinase from the species V. marismortui. The halo- and thermo-tolerant nature of the chitinolytic enzyme allows its potential use in agricultural and industrial applications. [less ▲]

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See detailBiopesticides: Feedback from distributors and users
Jijakli, Haissam ULg

Conference (2011, December)

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See detailBiocontrol proteomics:Implication of the pentoses phosphates pathway in the antagonist effect of Pichia anomala against Botrytis cinerea on apple
Kwasiborski, Anthony ULg; Renaut, Jenny; Lepoivre, Philippe ULg et al

Poster (2011, September 21)

The growing interest of the consumers for the wholesome food and the protection of the environment as well as the development of resistant pathogens to pesticides, stimulate the interest of growers to ... [more ▼]

The growing interest of the consumers for the wholesome food and the protection of the environment as well as the development of resistant pathogens to pesticides, stimulate the interest of growers to apply biological control methods. Pichia anomala strain K was previously identified as an efficient biocontrol agent of the main apple pathogens, Botrytis cinerea and Penicillum expansum. Further study demonstrated the complexicity of the mode of action of P. anomala against B. cinerea. A cDNA-AFLP and gene disruption study revealed implication of exo-β-1,3-glucanases in the mode of action of P. anomala strain Kh6 (a haploid form of P. anomala strain K displaying the same biocontrol properties). However, these studies suggested also implication of other factors. The present study aims to increase our knowledge of the mode of action of P. anomala strain Kh6 against B. cinerea using an in situ approach allowing the triple interaction, host/pathogen/antagonist and the proteomic tool allowing to study the ultime expression of the genome without a priori. One 50mm wound per apple were covered by a membrane and inoculated by a P. anomala suspension then by B. cinerea or not. Samples were collected during the exponential and stationary phase to identify the early and later responses to the presence of B. cinerea. After extraction, proteins were separated on 2-D gels. Spots influenced by the presence of B. cinerea in exponential and stationary phases were identified by MALDI-ToF. One hundred five and sixty spots of proteins were influenced by the presence of B. cinerea in exponential and stationary phases respectively. In exponential phase, P. anomala Kh6 in absence of B. cinerea uses mainly the glycolysis pathway, whereas in presence of pathogen, it orientates its energetic metabolism to the oxidative phosphorylation and sets up the pentose phosphate pathway. Thanks to this new orientation, P. anomala Kh6 probably obtains energy and nucleic acids allowing to colonize the wound as fast as in absence of B. cinerea and prevents the use of nutrients by the pathogen. In stationary phase, no differences in the P. anomala Kh6 energetic metabolism, in absence and in presence of B. cinerea were observed. During that phase, P. anomala Kh6 seems to use the alcoholic fermentation in order to face the nutrients impoverishment of the substrate. [less ▲]

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See detailBiocontrol proteomics:Implication of the pentoses phosphates pathway in the antagonist effect of Pichia anomala against Botrytis cinerea on apple.
Kwasiborski, Anthony ULg; renaut, Jenny; Delaplace, Pierre ULg et al

Conference (2011, August 31)

Pichia anomala strain K was previously identified as an efficient biocontrol agent of the apple pathogen, Botrytis cinerea. Further study demonstrated the complexicity of the mode of action of P. anomala ... [more ▼]

Pichia anomala strain K was previously identified as an efficient biocontrol agent of the apple pathogen, Botrytis cinerea. Further study demonstrated the complexicity of the mode of action of P. anomala against B. cinerea. A molecular study revealed implication of exo-β-1,3-glucanases in the mode of action of P. anomala but suggested implication of other factors. The present study aims to increase our knowledge of the mode of action of P. anomala strain Kh6 against B. cinerea using an in situ approach allowing interactions between organisms, and the proteomic tool allowing to study the ultime expression of the genome. One wound per apple were covered by a membrane and inoculated by P. anomala then by B. cinerea or not. Samples were collected at different incubation times to identify the responses of P. anomala to the presence of B. cinerea. After extraction, proteins were separated on 2-D gels. Spots influenced were identified by MALDI-ToF. In exponential phase, P. anomala Kh6 orientates its energetic metabolism from the glycolysis to the oxidative phosphorylation and sets up the pentose phosphate pathway. Thanks to this new pathway, P. anomala probably obtains energy and nucleic acids allowing to colonize the wound as fast as in absence of B. cinerea. In stationary phase, no differences in the P. anomala energetic metabolism, in absence and in presence of B. cinerea were observed. During that phase, P. anomala seems to use the alcoholic fermentation in order to face the nutrients impoverishment of the substrate. [less ▲]

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See detailAlternaria jacinthicola, a new fungal species causing blight leaf disease on water hyacinth (Eichhornia crassipes (Martius) Solms-Laubach)
Dagno, Karim ULg; Crovadore, Julien; Lefort, François et al

in Journal of Yeast and Fungal Research (2011), 2(7), 99-105

Water hyacinth (Eichhornia crassipes) causes environmental, agricultural and health problems in Mali. This is particularly severe in the District of Bamako and the irrigation systems of the “Office du ... [more ▼]

Water hyacinth (Eichhornia crassipes) causes environmental, agricultural and health problems in Mali. This is particularly severe in the District of Bamako and the irrigation systems of the “Office du Niger”area. During two years survey for fungal pathogens of water hyacinth infested areas, isolate Mlb684 was collected from diseased plant. This fungal isolate was identified as a potential mycoherbicide for sustainable management for water hyacinth. The aim of this study was to characterize isolate Mlb684. The characterization was based on a morphological description and a DNA sequence analysis. Various genes amplified from isolate Mlb684 were compared to those existing in Genbank. These genes were 18S ribosomal rDNA gene, ITS rDNA gene, elongation factor-1 alpha (EF1a) gene, calmodulin and actin genes. DNA sequence comparisons and morphological description provided enough evidences to show that isolate Mlb684 belonged to the Alternaria genus and was distinct from any other known Alternaria species. Based on these evidences, the new fungal isolate was called “Alternaria jacinthicola Dagno & M.H. Jijakli”. A specimen culture has been deposited in the Gembloux Agro Bio Tech Plant Pathology unit fungal collection, with Mlb684 reference and in the Industrial Fungal and Yeast Collection (BCCM/MUCL, Belgium) under the accession number: MUCL 53159 and all DNA sequences were deposited in GenBank (NCBI). [less ▲]

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