References of "Jijakli, Haissam"
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See detailPichia anomala and Candida oleophila in Biocontrol of Postharvest Diseases of Fruits: 20 Years of Fundamental and Practical Research
Massart, Sébastien ULg; Jijakli, Haissam ULg

in Prusky, D.; Gullino, ML. (Eds.) Plant Pathology in the 21st Century: Contributions to the 10th International Congress of Plant Pathology (2014)

The economic losses caused by post-harvest pathogens of apple and pear can still reach 25 %. There is currently an increasing demand to develop sustainable methods to control these post-harvest pathogens ... [more ▼]

The economic losses caused by post-harvest pathogens of apple and pear can still reach 25 %. There is currently an increasing demand to develop sustainable methods to control these post-harvest pathogens. Biocontrol agents are interesting candidates to answer this demand. Nevertheless, their commercial development is sometimes hampered by a low or non-reliable efficacy comparing to fungicide treatments. Fundamental research on the mode of action of the BCA and of its ecological fitness could help to overcome that phenomenon. This chapter reviews the progresses made during two decades to understand the mode of action and the ecological niche of two BCA, Pichia anomala strain K and Candida oleophila strain O. These advances required the combination of various methodologies (in vitro and in situ) and techniques (microbiology, microscopy, genome characterization, transcriptome, proteome, gene disruption. . .) which are summarized here. Importantly, the practical impact of these discoveries to improve the efficacy of the biopesticide is also highlighted. [less ▲]

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See detailCurrent impact and future directions of high throughput sequencing in plant virus diagnostics.
Massart, Sébastien ULg; Olmos, Antonio; Jijakli, Haissam ULg et al

in Virus Research (2014), 188

The ability to provide a fast, inexpensive and reliable diagnostic for any given viral infection is a key parameter in efforts to fight and control these ubiquitous pathogens. The recent developments of ... [more ▼]

The ability to provide a fast, inexpensive and reliable diagnostic for any given viral infection is a key parameter in efforts to fight and control these ubiquitous pathogens. The recent developments of highthroughput sequencing (also called Next Generation Sequencing – NGS) technologies and bioinformatics have drastically changed the research on viral pathogens. It is now raising a growing interest for virus diagnostics. This review provides a snapshot vision on the current use and impact of high throughput sequencing approaches in plant virus characterization. More specifically, this review highlights the potential of these new technologies and their interplay with current protocols in the future of molecular diagnostic of plant viruses. The current limitations that will need to be addressed for a wider adoption of high-throughput sequencing in plant virus diagnostics are thoroughly discussed. [less ▲]

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See detailControl of citrus blue mold by the antagonist yeast Pichia guilliermondii Z1: compatibility with commercial fruit waxes and putative mechanisms of action.
Lahlali, Rachid; Hamadi, Younes; Drider, R. et al

in Food Control (2014), 45

Pichia guilliermondii strain Z1, which was previously proven to be effective against blue mold of citrus fruit, has been further tested in controlled conditions to determine whether the yeast, as an ... [more ▼]

Pichia guilliermondii strain Z1, which was previously proven to be effective against blue mold of citrus fruit, has been further tested in controlled conditions to determine whether the yeast, as an alternative for synthetic fungicides, would be compatible with other postharvest practices used commercially. In particular, commercial fruit waxes can reduce their survival and effectiveness. The commercial fruit waxes tested, in combination at 20% with strain Z1, included microcrystalline, ester gum, candelilla, beeswax, montan, paraffin, rice bran, rosin maleic, carnauba, shellac, and one mixture shellac plus carnauba. Beeswax, paraffin, rosin maleic, carnauba, and shellac increased significantly strain Z1 survival in Petri dish assays. Candelilla, beeswax, rice bran, rosin maleic, carnauba, shellac, and shellacecarnauba mixture did not significantly reduce the strain Z1 yield on orange fruit surfaces compared to other waxes. With the exception of rosin maleic wax, none of the commercial fruit waxes or mixture increased significantly the ability of the formulated product of strain Z1 to control the postharvest pathogen Penicillium italicum on wounded orange fruit. When the formulated product of strain Z1 was used in combination with beeswax, strain Z1 retained the same efficacy. In contrast, microcrystalline, ester gum, candelilla, montan, paraffin, rice bran, carnauba, shellac, and shellac-carnauba mixture significantly reduced the effectiveness of the formulated product of strain Z1. No antibiosis was detected for strain Z1 against P. italicum. Strain Z1 inhibited the spore germination for the low juice concentration (up to 5%) when compared to the control. However, the addition of fresh juice after antagonist removal allowed the restoration of the germination of P. italicum spores; suggesting the possibility of competition for nutrients in the biocontrol activity of strain Z1. The study demonstrates the potential commercial application of strain Z1 with beeswax and rosin maleic wax for postharvest control of citrus blue mold. As biocontrol relies on competition for nutrients, an enriched formulation with nutrients is needed for reliable antifungal activity of this yeast strain [less ▲]

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See detailINCIDENCE DES MALADIES BACTERIENNES ET FONGIQUES DU RIZ A MADAGASCAR
Mamiharisoa Razanakoto, Léa ULg; De Clerck, Caroline ULg; Massart, Sébastien ULg et al

Poster (2013, December)

Le riz, c’est à la fois la culture la plus cultivée à Madagascar mais aussi la principale base de l’alimentation des malgaches. Même si le pays est à vocation agricole, l’autosuffisance alimentaire n’a ... [more ▼]

Le riz, c’est à la fois la culture la plus cultivée à Madagascar mais aussi la principale base de l’alimentation des malgaches. Même si le pays est à vocation agricole, l’autosuffisance alimentaire n’a jamais été atteinte, et l’importation de riz est toujours importante chaque année. Pour le riz, les maladies constituent le facteur entraînant la mauvaise qualité et la réduction des récoltes. C’est la raison qui nous amène à réaliser le suivi de la maladie de riz présent à Madagascar. L’incidence de la maladie a été évalué par l’observation visuelle des symptômes au champ et l’identité des pathogènes a été confirmée par les tests au laboratoire. Pour cela un échantillonnage a été réalisé dans les régions de Vakinankaratra, Analamanga et Atsinanana. Une enquête a été réalisée durant l’échantillonnage pour récolter les informations qui peuvent nous aider à l’identification de l’agent pathogène. L’isolement a été réalisé en utilisant les milieux standards pour bactéries et pour champignons. Les isolats ont été ensuite identifiés par l’observation des colonies sur boite de Pétri, par des observations microscopique des cellules en présence de différents colorants. Elles ont ensuite été identifiées par analyse moléculaire par séquençage.Dans les régions du haut plateau : les symptômes trouvés sont très variés et l’incidence de chaque symptôme est faible sauf le flétrissement des feuilles de riz. Dans la région Atsinanana, les symptômes trouvés ne varient pas et l’incidence est proche de 100 %. L’isolement des microorganismes à partir d’échantillons de chacune de ces régions confirme les résultats obtenus : Xanthomonas oryzae pv. oryzae correspondait à l’espèce pathogène dominante pour les hauts plateaux tandis que Curvularia lunata correspondait à l’espèce pathogène dominante pourAtsinanana. Ces différences trouvent probablement leur origine dans les conditions climatiques et le mode de culture très différentes entre cette région côtière et les hauts plateaux. [less ▲]

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See detailAgriculture: Des déjections de poissons comme engrais
Jijakli, Haissam ULg

Article for general public (2013)

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See detailDes déjections de poissons comme engrais pour plantes
Jijakli, Haissam ULg

Article for general public (2013)

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See detailGembloux: des poissons pour faire pousser des salades
Jijakli, Haissam ULg

Article for general public (2013)

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See detailreportage gembloux: agriculture en ville2
Jijakli, Haissam ULg

Speech/Talk (2013)

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See detailreportage Gembloux: agriculture en ville 1
Jijakli, Haissam ULg

Speech/Talk (2013)

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See detailSoigner les maux de l'orge à la racine
Fiers, Marie; Jijakli, Haissam ULg

Article for general public (2013)

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See detailEliciteurs dérivés de rhamnolipides : synthèses, modélisations et activités biologiques
Mayon, Patrick; Ait Barka, Essaid; Baillieul, Fabienne et al

Poster (2013, July 04)

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See detailDevelopment of formulated elicitors to control bioagressors of wheat
Le Mire, Géraldine ULg; Deleu, Magali ULg; Ongena, Marc ULg et al

Scientific conference (2013, July)

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See detailA new semi-selective agar medium for recovery and enumeration of antagonistic yeast, Pichia guilliermondii strain Z1 from orange fruit surface
Lahlali, Rachid; Hamadi, Younes; El Guilli, Mohamed et al

in African Journal of Microbiology Research [=AJMR] (2013), 7(25), 3209-3216

The aim of this work was to develop a semi-selective medium for recovery and enumeration of Pichia guilliermondii strain Z1, a reliable biocontrol agent against postharvest pathogens of citrus fruit, and ... [more ▼]

The aim of this work was to develop a semi-selective medium for recovery and enumeration of Pichia guilliermondii strain Z1, a reliable biocontrol agent against postharvest pathogens of citrus fruit, and to assess the population dynamic of this antagonistic yeast on orange fruit in relation to incubation temperature and time of incubation. PDA is the basal medium used in this study which allows the antagonistic strain Z1 source of carbon and nutrients. Different chemicals (thiophanate-methyl, thiabendazole, thiram and imazalil) and antibiotics (hygromycin, tetracyclin, ampicillin and chloramphenicol) have been tested individually and based on the plating efficiency up to 90%, 40 combinations have been assayed between antibiotics and fungicides. Ten combinations proved to be highly selective against citrus pathogens and laboratory microflora, but only one consisting of tetracyclin (1 g/L) and thiabendazole (60 mg/L) was retained based on plating efficiency up to 99%, total selectivity against laboratory microflora, epiphytic microflora from washed orange fruit and lower cost. The semi-selective medium TET-TBZ-PDA has been used to assess the impact of temperature and incubation time on the survival of strain Z1. It appears that population density was significantly influenced by both factors and the highest population size was recorded at 25°C followed by 5 and 35°C, respectively. This strain required a time of adaptation before entering the exponential growth phase with a maximum growth observed at 25°C relative to others. The semi-selective medium TETTBZ- PDA could be an efficient and valuable way to track the population density of this strain on the surface of orange when applied pre-or postharvest. This semi-selective medium may also aid in reaching a population density allowing a better efficiency in relation to environmental conditions. [less ▲]

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See detailFirst report of banana mild mosaic virus infecting plantain in Ivory Coast
Kouadio, Kouakou Théodore ULg; Agneroh, Thérèse Atcham; De Clerck, Caroline ULg et al

in Plant Disease (2013), 97(5), 693

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