References of "Jijakli, Haissam"
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See detailElicitor screening to protect winter wheat against Septoria tritici Blotch: preliminary results
Le Mire, Géraldine ULg; Deleu, Magali ULg; Ongena, Marc ULg et al

Conference (2014, September)

Elicitors are plant immunity-triggering compounds which are currently considered as one of the most promising tools in agriculture for the induction of plant resistance to various diseases (Mejía-Teniente ... [more ▼]

Elicitors are plant immunity-triggering compounds which are currently considered as one of the most promising tools in agriculture for the induction of plant resistance to various diseases (Mejía-Teniente et al., 2010). By contributing to both economic and environmental performances of agroecosystems, they can help reducing the use of chemical inputs. Although some elicitor products are already available on the market, it appears that a variable efficiency in the field, along with an uneasy integration in the current legislation and agricultural strategies, make these tools difficult to use (Walters, Ratsep, & Havis, 2013). Besides these limitations, few elicitor treatments have yet been efficiently and specifically designed to protect crop plants such as wheat, which is grown and consumed worldwide, against major diseases threatening both their yield and quality. There is a strong need to better understand the mechanisms of induced resistance in plants and develop elicitor use in agriculture. A phD research is currently led in Gembloux Agro Bio-Tech to develop a method based on eliciting agents, tested in greenhouse and field conditions, to protect winter wheat against major diseases, namely Septoria tritici, Fusarium graminearum and Fusarium culmorum. This project focuses on the screening of a large number of elicitors from different origins and structures. In 2014, we focused the first screening experiments on the protection of wheat against Septoria tritici Blotch (STB). Two winter wheat genotypes were tested: susceptible ‘Avatar’ and semi-resistant ‘Sy Epson’. Plants at 3-leaf stage were first sprayed till runoff with different concentrations of elicitors, and then inoculated 5 days later with a Septoria tritici spore suspension (106 spores mL-1) using a hand sprayer. Control plants were treated, prior to disease inoculation, with sterile water (negative control) or with BION® (ASM, Syngenta Europe; positive control). The disease severity and incidence were scored every 2 days for 28 days post-inoculation (d.p.i) by measuring the percentage of area covered with lesions and bearing pycnidia on the third leaf. The first results of these tests should enable a first discrimination of elicitors considering their dose-efficiency to reduce disease severity. After further screening of all the elicitors in hand, a determination of the elicitation pathways of the most efficient molecules tested will be undertaken. We intend to study the influence of various factors (i.e Temperature, relative humidity, plant development stage) on the elicitation potential and develop a formulation to be finally tested under field conditions. [less ▲]

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See detailMode of Action of Lactoperoxidase as Related to Its Antimicrobial Activity: A Review
Bafort, Françoise ULg; Parisi, Olivier ULg; Perraudin, Jean-Paul et al

in Enzyme Research (2014), 2014

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See detailL'aquaponie dans la boite. Equipements remarquables: la Paff Box en test à Gembloux
Jijakli, Haissam ULg

Article for general public (2014)

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See detailVirus indexing and sanitation of international banana collection: insights from a 5-year collaborative effort
De Clerck, Caroline ULg; Parmenter, Kathleen; Vandenhouwe, Ines et al

Conference (2014, August 14)

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See detailBelowground Chemical Ecology: The Case of Wireworms
Barsics, Fanny ULg; Delory, Benjamin ULg; Delaplace, Pierre ULg et al

Poster (2014, June)

Wireworms, clock-beetles' larvae (Coleoptera, Elateridae), are below ground pests of many crops. They cause dramatic yield losses worldwide. Research on their ecology is crucial to undertake innovative ... [more ▼]

Wireworms, clock-beetles' larvae (Coleoptera, Elateridae), are below ground pests of many crops. They cause dramatic yield losses worldwide. Research on their ecology is crucial to undertake innovative management strategies. In the field of chemical ecology, multitrophic interactions occurring in the rhizosphere are gaining increasing attention from entomologists and agronomists. Our research aims at unveiling the role of volatile organic compounds (VOC) involved in wireworms' foraging behavior, putatively leading to host localization and/or host recognition. [less ▲]

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See detailA methodology for elicitor screening of winter wheat infected by STB and FHB
Le Mire, Géraldine ULg; SIAH, Ali; Deleu, Magali ULg et al

Poster (2014, May 20)

Un doctorat au sein de Gembloux Agro Bio-Tech (Plateforme Agriculture Is Life) vise à développer une méthode à base d'éliciteurs bio-sourcés pour protéger le blé d'hivers contre la Septoriose (STB) et la ... [more ▼]

Un doctorat au sein de Gembloux Agro Bio-Tech (Plateforme Agriculture Is Life) vise à développer une méthode à base d'éliciteurs bio-sourcés pour protéger le blé d'hivers contre la Septoriose (STB) et la Fusariose (FHB). Dans ce cadre, la mise au point d'un protocole efficace d'infection du blé est élémentaire avant de pouvoir entamer les tests de screening d'éliciteurs. Nous présentons ici une méthode d'infection du blé par Septoria tritici et les résultats de ces tests de reproduction de symptômes. [less ▲]

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See detailHigh throughput sequencing of aphid haemolymph reveals endosymbiont diversity
De Clerck, Caroline ULg; Leonard, Simon; Joncour, Pauline et al

Conference (2014, May 20)

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See detailIdentification of Metabolic Pathways Expressed by Pichia anomala KH6 in the Presence of the Pathogen Botrytis cinerea on Apple: New Possible Targets for Biocontrol Improvement
Kwasiborski, Anthony; Bajji, Mohammed; Renaut, J. et al

in PLoS ONE (2014), 9(3),

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See detailSreening of essential oils against rice pathogens isolated from Madagascar
Mamiharisoa Razanakoto, Léa ULg; De Clerck, Caroline ULg; Massart, Sébastien ULg et al

Poster (2014, February 07)

Four predominant isolated pathogens have been identified during survey of rice diseases in three regions in Madagascar. These pathogens were the bacteria Xanthomonas oryzae pv. oryzae and Pseudomonas ... [more ▼]

Four predominant isolated pathogens have been identified during survey of rice diseases in three regions in Madagascar. These pathogens were the bacteria Xanthomonas oryzae pv. oryzae and Pseudomonas fuscovaginae and the fungi Alternaria sp. and Curvularia lunata. These pathogens are causing significant yield reduction on rice, which is the staple food in this country. After the identification of those pathogens, the objective of this work was to identify appropriate control measures against them. Plants extracts has been traditionally used to control diseases in other plant species. Nowadays, the problem of residues of phytopharmaceutical product, reinforce the need for research on the development of natural plant extracts to control plant diseases. Madagascar has many endemic plant of interest and we have evaluated the antimicrobial properties of essential oils from Malagasy plants. The main object of this study is the in-vitro screening of essential oils to control the pathogens isolated on rice in Madagascar. Thirty nine essentials oils from Madagascar had been tested. Activity of essentials oils on Pseudomonas fuscovaginae, Alternaria sp. and Curvularia lunata had been tested using broth media and on agar medium for Xanthomonas oryzae pv. oryzae. The growth of the pathogen (bacteria and conidia) in the presence of the essential oil was evaluated and compared to a control. Nine of the 39 essentials oils showed interesting antimicrobial activity with an efficiency more than 70% against at least one bacterial pathogen. One essential oil inhibited the growth of the two pathogens tested. For fungal pathogens, three essential oils presented an efficiency of more than 70% against at least one fungal pathogen. These results confirm that some essential oils present an interesting antimicrobial activity. The in-situ confirmation on plant of this activity is currently ongoing. [less ▲]

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See detailDevelopment of formulated elicitors to control bioagressors of wheat: which interests?
Le Mire, Géraldine ULg; Deleu, Magali ULg; Ongena, Marc ULg et al

Poster (2014, February 07)

Crop plants are constantly exposed to a wide range of environmental stresses that can cause major productivity loss. Fungicide treatments are the most efficient disease control methods used up to now ... [more ▼]

Crop plants are constantly exposed to a wide range of environmental stresses that can cause major productivity loss. Fungicide treatments are the most efficient disease control methods used up to now. However, the environmental and health issues caused by these chemicals make it urgent to develop safer methods for pathogen control. In the optic of a sustainable agriculture, several biocontrol tools have been developed during the last decades. Elicitors are increasingly subject to research as interesting alternatives to phytosanitary products. Yet, few efficient formulations exist on the market, and no product has been designed specifically to protect wheat crops. This study will thus focus on the the manipulation of host natural defenses through the use of elicitors as a preventive treatment against major wheat diseases such as Septoria tritici, Fusarium graminearum and Fusarium culmorum. [less ▲]

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See detailBiofilter in aquaponics
Delaide, Boris ULg; Lambrechts, Paul Emile ULg; Willaert, Jean-François ULg et al

Poster (2014, February 07)

Aquaponics is a combination of aquaculture (fish farming) and hydroponics (horticulture). In this concept, the fish faeces and excreted ammonia are partially transformed into soluble nutrients which then ... [more ▼]

Aquaponics is a combination of aquaculture (fish farming) and hydroponics (horticulture). In this concept, the fish faeces and excreted ammonia are partially transformed into soluble nutrients which then are available for plants. Wastewater from aquaculture is filtrated by plants and return to the fish tank in a loop cycle. This strongly reduces the use of water and increases the economic efficiency with an additional production of plant, e.g. vegetables. From the hydroponics view, this combination allows important decreases in chemical nutrients use for plant grow. A central part of this system is the biofilter. The microorganisms that colonize it are essential to process the fish waste into soluble nutrients easily assimilated by plants. During this process, nitrifying bacteria convert free ammonia into nitrite and then nitrate. The first objective of this research will concern the identification and isolation of the main microorganism species involved by the dilution plating and metagenomics technics. The optimal conditions to process the fish waste will be determined for such microorganism. The efficiency of nitrite and nitrate production by these micro-organisms will be tested in different conditions (T°, pH, O2, water flow, support, etc..) in vitro at the lab scale and in aquaponics prototypes. [less ▲]

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See detailInteractions of a potential plant elicitor mannolipid with plant model membranes
Polo Lozano, Damien ULg; Lins, Laurence ULg; Ongena, Marc ULg et al

Poster (2014, February 07)

The use of chemical pesticides causes problems for human health and environment. In this context, there is an increasing interest for alternative products such as biopesticides. Among them, elicitors act ... [more ▼]

The use of chemical pesticides causes problems for human health and environment. In this context, there is an increasing interest for alternative products such as biopesticides. Among them, elicitors act on the plants by inducing systemic resistance against diseases caused by fungal, viral, bacterial agents and insects. The target of the elicitors is supposed to be the plant plasma membranes (PPM). The main mechanisms of interaction of many elicitors involve proteic receptors but lipid-based elicitors (LBE) may preferably interact with the lipidic fractions of PPM. However there is no detailed information at the molecular level on the PPM-LBE interactions. Our work is focused on a original synthetic LBE composed of a mannoside linked to a myristic acid. It has potential elicitor activities as shown by the assays on tobacco root cells. These activities could be related to its interaction with the lipidic phase of PPM. Since PPM are complex entities, the analyses of the PPM- molecule interactions are quite difficult. In this context, these interactions were carried out using biomimetic membranes of PPM such as Langmuir monolayers and multilayers. The effects of our molecule on these membranar systems were investigated by biophysical and in silico approaches. [less ▲]

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See detailNew alternatives to chemical pesticides: deciphering the action mechanisms of lipid based plant elicitors via complementary biophysical and biological approaches.
Nasir, Mehmet Nail ULg; Polo Lozano, Damien ULg; Luzuriaga Loaiza, Walter ULg et al

Poster (2014, February)

Nowadays, many health and environmental problems are caused by the use of chemical pesticides. In this context, an increasing demand for alternative products such as biopesticides has been observed. Among ... [more ▼]

Nowadays, many health and environmental problems are caused by the use of chemical pesticides. In this context, an increasing demand for alternative products such as biopesticides has been observed. Among biopesticides, elicitor molecules which are able to trigger immune defense responses in plants are one of the most promising options. Although numerous elicitors have been discovered, the mechanisms involved in the perception, by plants, of only a few molecules have been identified. These elicitors usually interact with proteic receptors but we have recently shown that they may also act on the lipid phase of the plasma membrane. This project first aims to improve our understanding of the molecular mechanisms involved in the recognition of specific lipid based elicitors (LBE). On that basis, the FIELD project will contribute to the design and the development of innovative compounds derived natural LBE. A multi-disciplinary approach, based on chemistry, bio-physics, bio-chemistry, and phytopathology will be followed by a consortium of different research groups from Gembloux Agro-Bio Tech in close collaboration with teams from foreign institutions. [less ▲]

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See detailCombination of Genomic and Proteomic Approaches to Characterize the Symbiotic Population of the Banana Aphid (Hemiptera: Aphididae)
De Clerck, Caroline ULg; Tsuchida, Tsutomu; Massart, Sébastien ULg et al

in Environmental Entomology (2014)

Aphids are known to live in symbiosis with speciÞc bacteria called endosymbionts that have positive or negative impacts on their hosts. In this study, six banana aphid (Pentalonia nigronervosa Coquerel ... [more ▼]

Aphids are known to live in symbiosis with speciÞc bacteria called endosymbionts that have positive or negative impacts on their hosts. In this study, six banana aphid (Pentalonia nigronervosa Coquerel) strains from various geographical origins (Gabon, Madagascar, and Burundi) were screened to determine their symbiotic content, using complementary genomic (16S rDNA sequencing and speciÞc polymerase chain reaction) and proteomic (two-dimensional difference gel electrophoresis coupled with protein identiÞcation by matrix assisted laser desorption-ionization time-of-ßight mass spectrometry) approaches. Despite the geographical heterogeneity, the combined methods allowed us to identify the same two symbionts in the six aphids strains tested: Buchnera aphidicola and Wolbachia. Although B. aphidicola is found in almost all aphid species, the systematic presence of Wolbachia in banana aphids is particularly interesting, as this bacterium usually has a low prevalence in aphid species. Phylogenetic analyses showed that the Wolbachia sp. strain found in P. nigronervosa was very similar to the strain present in aphids of the genus Cinara, known to have developed a strong and long-term symbiotic association with Wolbachia. The high level of asexual reproduction in P. nigronervosa could be linked to the presence of Wolbachia, but its prevalence also suggests that this symbiotic bacterium could play a more essential role in its aphid host. [less ▲]

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See detailDevelopment of a colorimetric method for the dosage of OI- anions and I2 in aqueous media
Bafort, Françoise ULg; Barthelemy, Jean-Paul ULg; Parisi, Olivier ULg et al

in Communications in Agricultural and Applied Biological Sciences (2014), 79(1), 155-160

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See detailThe ability of the antagonist yeast Pichia guilliermondii strain Z1 to suppress green mould infection in citrus fruit
Lahlali, Rachid; Hamadi, Younes; El Guilli, Mohammed et al

in Italian Journal of Food Safety (2014), 3(4), 265-268

Detailed reference viewed: 16 (0 ULg)