References of "Jijakli, Haissam"
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See detailA Simple And Rapid Protocol Of Crude Dna Extraction From Apple Trees For Pcr And Real-Time Pcr Detection Of 'Candidatus Phytoplasma Mali'
Aldaghi, M.; Massart, Sébastien ULg; Dutrecq, O. et al

in Journal of Virological Methods (2009), 156(1-2), 96-101

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See detailBiological control of grey mould in strawberry fruits by halophilic bacteria
Essghaier, Badiaa; Fardeau, ML; Cayol, JL et al

in Journal of Applied Microbiology (2009), 106(3), 833-846

Aims: Grey mould caused by Botrytis cinerea is an economically important disease of strawberries in Tunisia and worldwide. The aim of this study was to select effective halophilic bacteria from ... [more ▼]

Aims: Grey mould caused by Botrytis cinerea is an economically important disease of strawberries in Tunisia and worldwide. The aim of this study was to select effective halophilic bacteria from hypersaline ecosystems and evaluate the abilities of antifungal bacteria to secrete extracellular hydrolytic enzymes, anti- Botrytis metabolites and volatiles. Methods and Results: Grey mould was reduced in strawberry fruits treated with halophilic antagonists and artificially inoculated with B. cinerea. Thirty strains (20Æ2%) were active against the pathogen and reduced the percentage of fruits infected after 3 days of storage at 20 C, from 50% to 91Æ66%. The antagonists were characterized by phenotypic tests and 16S rDNA sequencing. They were identified as belonging to one of the species: Virgibacillus marismortui, B. subtilis, B. pumilus, B. licheniformis, Terribacillus halophilus, Halomonas elongata, Planococcus rifietoensis, Staphylococcus equorum and Staphylococcus sp. The effective isolates were tested for antifungal secondary metabolites. Conclusions: Moderately halophilic bacteria may be useful in biological control against this pathogen during postharvest storage of strawberries. Significance and Impact of the study: The use of such bacteria may constitute an important alternative to synthetic fungicides. These moderate halophiles can be exploited in commercial production and application of the effective strains under storage and greenhouse conditions. [less ▲]

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See detailAssessment Of Pichia Anomala (Strain K) Efficacy Against Blue Mould Of Apples When Applied Pre- Or Post-Harvest Under Laboratory Conditions And In Orchard Trials
Lahlali, R.; Massart, Sébastien ULg; De Clercq, D. et al

in European Journal of Plant Pathology (2009), 123(1), 37-45

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See detailDevelopment of simple molecular protocols to detect Banana bunchy top virus with the phytoPaSS System
Busogoro, Jean-Pierre; Dutrecq, Olivier; Jijakli, Haissam ULg

in Acta Horticulturae (2009), 828

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See detailLa banane: de son origine à sa commercialisation
Lassois, Ludivine ULg; Busogoro, J. P.; Jijakli, Haissam ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2009), 13(4), 575-586

Cultivated bananas are giant herbaceous plants within the genus Musa. They are both sterile and parthenocarpic. There are well over a thousand domesticated Musa cultivars, they are mostly triploid (a few ... [more ▼]

Cultivated bananas are giant herbaceous plants within the genus Musa. They are both sterile and parthenocarpic. There are well over a thousand domesticated Musa cultivars, they are mostly triploid (a few are diploid or tetraploid) and are derived from crosses between two wild species, Musa acuminata and Musa balbisiana. In terms of production, bananas are the fourth agricultural product after rice, wheat, and maize. They constitute the basis of food security for many people. Cropping systems vary widely around the world and contrasting objectives are encountered: consumption by the producer, sale on local or national markets, export, etc. Cooking bananas, including plantains, must be distinguished from dessert bananas, which constitute a major international trade. This international trade started only in the early 1900s but it has since grown continuously. Banana is currently the most exported fruit, in terms of both value and quantity. Despite the high genetic diversity found within the genus Musa, the export market is mainly based on single Cavendish. There are major challenges to banana production from biotic or abiotic stresses to continue to meet the criteria of sustainability, quality and yield that are imposed. [less ▲]

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See detailCombining an original method for preserving RNA expression in situ with an effetive RNA method makes it possible to study gene expression in any banana fruit tissue.
Lassois, Ludivine ULg; de Lapeyre de Bellaire, Luc; Jijakli, Haissam ULg

in Fruits (2009), 64(3), 127-137

Introduction. RNA isolation is a prerequisite to studying gene expression in banana and to understanding changes occurring in response to the environment. Standard extraction methods do not efficiently ... [more ▼]

Introduction. RNA isolation is a prerequisite to studying gene expression in banana and to understanding changes occurring in response to the environment. Standard extraction methods do not efficiently extract RNA from plants such as banana, with high levels of phenolics, carbohydrates, or other compounds that bind to and/or coprecipitate with RNA. Materials and methods. Five to seven RNA extraction methods were compared. Four crowntissue storage methods were also compared. cDNA-AFLP was used to ensure that the obtained RNA was of sufficient quality for molecular applications and that RNA expression was unaltered by in situ storage. Results and discussion. The modified hot-borate method proved to be the best RNA extraction method, allowing high yields of good quality, undegraded RNA from the crown, fruit peel and pulp at all stages of ripening. The RNA obtained by this method was of sufficient quality for molecular applications such as cDNA-AFLP that give highly reproducible results. Freeze-drying of fresh tissues and tissue conservation in hot-borate buffer, two original storage methods, appear appropriate for preserving RNA in situ without ultra-low temperature. The RNA obtained was of high quality, undegraded, and useful for all downstream applications. The genome expression profile obtained by cDNA-AFLP analysis was unaltered by these methods for storing collected tissues. Conclusion. By applying all the suggested procedures in this work, it is possible to store and study gene expression in any banana fruit tissue, whatever the maturity stage, without affecting the RNA expression level. [less ▲]

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See detailevaluation of two protein extractions protocols for pichia anomala proteome analysis
Bajji, M.; Delaplace, Pierre ULg; Mauro, S. et al

Poster (2009)

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See detailThe post-harvest quality of bananas is determined by pre-harvest factors
de Lapeyre de Bellaire, Luc; Chillet, Marc; Lassois, Ludivine ULg et al

Poster (2009)

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See detailUse of cDNA-AFLP to study the defence-related gene expression in bananas (Musa spp.), inoculated with Colletotrichum musae responsible of crown rot
Lassois, Ludivine ULg; de Lapeyre de Bellaire, Luc; Jijakli, Haissam ULg

Conference (2009)

Crown rot disease of bananas is widespread in producing countries and is considered as the most important post-harvest disease of exported bananas. Variations of susceptibility to the disease have been ... [more ▼]

Crown rot disease of bananas is widespread in producing countries and is considered as the most important post-harvest disease of exported bananas. Variations of susceptibility to the disease have been noted between bananas but the origins still unknown. The biological responses of the fruit, including physiological change and disease susceptibility are controlled and regulated by gene expression. One way to understanding the reactions involved in variation of banana susceptibility to the disease in relation to their physiological state, is to study the expression of genes involved in these processes. To this purpose, crown sample previously inoculated with C. musae and showing 2 levels of susceptibility (very high and very low) were collected to be compared. Crown sample of each susceptibility level was collected at two different maturity stages: at harvest and 13 days after harvest (3 days after ripening). Collected crowns were immediately freeze-dried, an original method to conserve gene expression. cDNA-AFLP was applied on these 4 cell populations in order to highlight the differential transcription of genes whose function is "a priori" unknown. The cDNA-AFLP result was confirmed using quantitative real-time reverse transcription PCR. Various defence-related genes were identified and will be presented. [less ▲]

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See detailcDNA-AFLP analysis of Candida oleophila (strain O) genes differentially expressed during the biocontrol of Botrytis cinerea on harvested apples
Bajji, Mohammed; Jijakli, Haissam ULg

in Bulletin OILB/SROP = IOBC/WPRS Bulletin (2009), 43

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See detailInterlaboratory Evaluation Of Two Reverse-Transcriptase Polymeric Chain Reaction-Based Methods For Detection Of Four Fruit Tree Viruses
Massart, Sébastien ULg; Brostaux, Yves ULg; Barbarossa, L. et al

in Annals of Applied Biology (2009), 154(1), 133-141

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See detailImplication de la compétition pour les acides aminés dans le contrôle biologique de la pourriture bleue des pommes en post-récolte
Krimi Bencheqroun, S.; Bajji, M.; Labhilili, M. et al

Conference (2008, April)

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See detailPreliminary evaluation of antimicrobial activity of some chemicals on in vitro apple shoots infected by 'Candidatus Phytoplasma mali'.
Aldaghi, M.; Massart, Sébastien ULg; Druart, Ph et al

in Communications in Agricultural and Applied Biological Sciences (2008), 73(2),

Detailed reference viewed: 57 (10 ULg)