References of "Jijakli, Haissam"
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See detailEffect of temperature and water activity on spore germination and mycelial growth of three fungal biocontrol agents against water hyacinth (Eichhornia crassipes)
Dagno, Karim ULg; Lahlali, Rachid; Diourté, M. et al

in Journal of Applied Microbiology (2011), 110

The fungi were grown in vitro on plates containing potato dextrose agar medium at different aw values (glycerol being added to adjust the aw). The percentage of viable conidia and radial growth rate ... [more ▼]

The fungi were grown in vitro on plates containing potato dextrose agar medium at different aw values (glycerol being added to adjust the aw). The percentage of viable conidia and radial growth rate decreased with decreasing water activity. Statistical analysis showed a significant effect of aw, temperature and the aw temperature interaction on mycelial growth (P < 0Æ0001). Water activity emerged as the factor exerting the greatest influence. Differences were observed between the fungi tested, the C. malorum appearing more tolerant to low aw and the F. sacchari more tolerant to high temperature (35 C). Growth models predicting the combined effect of aw and temperature were developed and response surfaces generated, showing fairly good agreement with the experimental values. Conclusions: Our results confirm the previous finding that aw has a greater influence than temperature on fungal growth. Under most conditions, variation of environmental factors has a detrimental influence on the percentage of viable conidia and mycelial growth rate of fungal isolates. [less ▲]

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See detailDevelopment of a Quick Quantitative Real-Time PCR for the In Vivo Detection and Quantification of Peach latent mosaic viroid
Parisi, Olivier ULg; Lepoivre, Philippe ULg; Jijakli, Haissam ULg

in Plant Disease (2011), 95(2), 137-142

Viroids are plant pathogens infecting a broad range of herbaceous and tree crops. Among them, the Peach latent mosaic viroid (PLMVd) infects mainly peach trees, causing a loss of production with no ... [more ▼]

Viroids are plant pathogens infecting a broad range of herbaceous and tree crops. Among them, the Peach latent mosaic viroid (PLMVd) infects mainly peach trees, causing a loss of production with no curative options. Detecting this viroid is thus important for certification procedures aiming to avoid the release of infected material into orchards. Presented here is a complete detection method based on reverse transcription (RT) followed by a quantitative real-time polymerase chain reaction (PCR). New primers were selected and optimal reaction conditions determined for routine application of the method. The technique is 105 times more sensitive than the endpoint RT-PCR used for PLMVd detection, and permits earlier detection of PLMVd in infected plants. The quick, low-cost extraction procedure used and the quality of the results obtained make this method suitable for routine testing. [less ▲]

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See detailCatecholamine biosynthesis pathway potentially involved in banana defense mechanisms to crown rot disease
Lassois, Ludivine ULg; De Clerck, Caroline ULg; Frettinger, Patrick et al

in Communications in Agricultural and Applied Biological Sciences (2011), 76(4), 591-601

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See detailPredictive modelling of the combined effect of temperature and water activity (aw) on the in vitro growth of Erwinia spp infecting potato tubers in Belgium
Moh, Augustin; Massart, Sébastien ULg; Lahlali, Rachid et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2011), 15(3), 378-386

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See detailApple stem grooving virus
Massart, Sébastien ULg; Jijakli, Haissam ULg; Kummert, Jean

in Hadidi, A.; Barba, M.; Candresse, T. (Eds.) et al Virus and Virus-Like Diseases of Pome and Stone Fruits (2011)

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See detailUV protectants for Candida Oleophila (strain O), a biocontrol agent of post-harvest fruit disease
Lahlali, R.; Buonatesta, R.; Jijakli, Haissam ULg

in Plant Pathology (2011), 60(2), 288-295

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See detailEfficacy assessment of Pichia guilliermondii strain Z1, a new biocontrol agent, against citrus blue mould in Morocco under the influence of temperature and relative humidity
Lahlali, Rachid; Hamadi, Younes; El Guilli, Mohammed et al

in Biocontrol Science (2011), 56

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See detailEffects of Temperature and Relative Humidity on the In Vitro and In Vivo Radial Growth of Penicillium italicum and on the Biocontrol Activity of Pichia guilliermondii, Strain Z1
El Guilli, M.; Ibriz, M.; Lahlali, Rachid et al

in Acta Horticulturae (2011), 905

The objective of this study was to assess the effect of temperature (5-25°C) on the ‘in vitro’ and ‘in vivo’ growth rates of Penicillium italicum and to determine the combined effect of temperature and ... [more ▼]

The objective of this study was to assess the effect of temperature (5-25°C) on the ‘in vitro’ and ‘in vivo’ growth rates of Penicillium italicum and to determine the combined effect of temperature and relative humidity (45 to 100%) on lesion size of this pathogenic fungus on Valencia late oranges, either alone or in combination with the antagonistic yeast strain Z1 of Pichia guilliermondii Wickerham. Statistical analysis showed a significant effect of temperature on the ‘in vitro’ and ‘in vivo’ radial growth of P. italicum with the maximum growth observed at temperature of 25°C. In both cases, no growth was observed at a temperature of 35°C. These factors had a significant effect on P. italicum lesion size when it was applied alone on Valencia late oranges and insignificant when yeast strain Z1 was applied 24 h before P. italicum inoculation. Our results confirm previous ‘in vitro’ findings that aw has a greater influence than temperature on P. italicum growth and highlight that the strain Z1 showed high antagonistic potential against this pathogen over a range of temperature-relative humidity regimes favouring P. italicum development. [less ▲]

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See detailPichia anomala in biocontrol for apples: 20 years of fundamental research and practical applications
Jijakli, Haissam ULg

in Antonie van Leeuwenhoek (2011), 99(1), 93-105

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See detailIdentification of genes involved in the response of banana to crown rot disease
Lassois, Ludivine ULg; Frettinger, Patrick; de Lapeyre de Bellaire, Luc et al

in Molecular Plant-Microbe Interactions [=MPMI] (2011), 24(1), 143-153

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See detailNovel applications of lactoperoxydase system against pathogens of cultivated plants
Jijakli, Haissam ULg; Bafort, Françoise ULg; perraudin, J.P

Conference (2010, June)

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See detailLa lutte biologique et les terrains de sport et des espaces verts
Jijakli, Haissam ULg

Conference (2010, January)

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See detailLe developpement des methodes de lutte biologique en agriculture et horticulture
Jijakli, Haissam ULg

Conference (2010, January)

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See detailCrown rot of banana: Preharvest factors involved in postharvest disease development and integrated control methods
Lassois, Ludivine ULg; Jijakli, Haissam ULg; Chillet, M. et al

in Plant Disease (2010), 94(6), 648-658

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See detailHand position on the bunch and source-sink ratio influence the banana fruit susceptibility to crown rot disease
Lassois, Ludivine ULg; Bastiaanse, H.; Chillet, M. et al

in Annals of Applied Biology (2010), 156

The postharvest development of crown rot of bananas depends notably on the fruit susceptibility to this disease at harvest. It has been shown that fruit susceptibility to crown rot is variable and it was ... [more ▼]

The postharvest development of crown rot of bananas depends notably on the fruit susceptibility to this disease at harvest. It has been shown that fruit susceptibility to crown rot is variable and it was suggested that this depends on environmental preharvest factors. However, little is known about the preharvest factors influencing this susceptibility. The aim of this work was to evaluate the extent to which fruit filling characteristics during growth and the fruit development stage influence the banana susceptibility to crown rot. This involved evaluating the influence of (a) the fruit position at different levels of the banana bunch (hands) and (b) changing the source–sink ratio (So–Si ratio), on the fruit susceptibility to crown rot. The fruit susceptibility was determined by measuring the internal necrotic surface (INS) after artificial inoculation of Colletotrichum musae. A linear correlation (r = −0.95) was found between the hand position on the bunch and the INS. The So–Si ratio was found to influence the pomological characteristics of the fruits and their susceptibility to crown rot. Fruits of bunches from which six hands were removed (two hands remaining on the bunch) proved to be significantly less susceptible to crown rot (INS = 138.3 mm2) than those from bunches with eight hands (INS = 237.9 mm2). The banana susceptibility to crown rot is thus likely to be influenced by the fruit development stage and filling characteristics. The present results highlight the importance of standardising hand sampling on a bunch when testing fruit susceptibility to crown rot. They also show that hand removal in the field has advantages in the context of integrated pest management, making it possible to reduce fruit susceptibility to crown rot while increasing fruit size. [less ▲]

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See detailEvaluation of three essential oils as potential source of botanical fungicide
Kouassi, Kouadio Hugues Sosthène ULg; Bajji, M.; Zhiri, A. et al

Conference (2010)

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See detailProduction and partial characterization of chitinase from a halotolerant Planococcus rifitoensis strain M2-26
Essghaier, Badiaa; Rouaissi, Mustapha; Boudabous, Abdellatif et al

in World Journal of Microbiology & Biotechnology (2010), 26(6), 977-984

This paper is the first to investigate the production and partial characterization of the chitinase enzyme from a moderately halophilic bacterium Planococcus rifitoensis strain M2-26, earlier isolated ... [more ▼]

This paper is the first to investigate the production and partial characterization of the chitinase enzyme from a moderately halophilic bacterium Planococcus rifitoensis strain M2-26, earlier isolated from a shallow salt lake in Tunisia. The impact of salt, salinity concentration, pH, carbon and nitrogen sources on chitinase production and activity have been determined. This is the first report on a high salt-tolerant chitinase from P. rifitoensis, since it was active at high salinity (from 5 to 30% NaCl) as well as in the absence of salt. This enzyme showed optimal activity at 70 C and retained up to 82 and 66% of its original activity at 80 or 90 C, respectively. The activity of the enzyme was also shown over a wide pH range (from 5 to 11). For characterization of the enzyme activity, the chitinase secreted in the culture supernatant was partially purified. The preliminary study of the concentrated dialysed supernatant on native PAGE showed at least three chitinases produced by strain M2-26, with highest activity approximately at 65 kDa. Thus, the thermo-tolerant and high salt-tolerant chitinases produced by P. rifitoensis strain M2-26 could be useful for application in diverse areas such as biotechnology and agro-industry. [less ▲]

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See detailIntegrated control of crown rot of banana with Candida oleophila strain O, calcium chloride and modified atmosphere packaging
Bastiaanse, H.; de Lapeyre de Bellaire, Luc; Lassois, Ludivine ULg et al

in Biological Control (2010), 53

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See detailPlant-RNA viroid relationship: a complex host pathogen interaction
Parisi, Olivier ULg; Lepoivre, Philippe ULg; Jijakli, Haissam ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14(3),

Viroids are non encapsidated small RNA plant pathogens unable to produce any protein. They are able to infect dramatically a broad range of plants including herbaceous and tree crops. The ways by which ... [more ▼]

Viroids are non encapsidated small RNA plant pathogens unable to produce any protein. They are able to infect dramatically a broad range of plants including herbaceous and tree crops. The ways by which viroids are able to induce diseases are actually unknown. However, recent studies have shown that viroids are able to regulate the gene expression of their hosts, they can modify the host-protein phosphorylation sensibility and they interact with host-protein implicated RNA trafficking and protein phosphorylation. Moreover during their evolution plants have developed a mechanism able to regulate their gene expression and to degrade exogenous RNAs like viroids: the gene silencing. Unfortunately, this pathway seems, now, also highly implicated in the symptoms development. This review describes studies that are realized since a few years to increase the knowledge about the plant-viroid relationship. [less ▲]

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