References of "Jacques, Philippe"
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See detailComparative titration of ginsenosides by different techniques in commercial ginseng products and callus cultures
Kevers, Claire ULg; Jacques, Philippe; Gaspar, Thomas ULg et al

in Journal of Chromatographic Science (2004), 42(10, Nov-Dec), 554-558

The ginsenoside content of different ginseng species (Panax ginseng, P. quinquefolium, and P. vietnamensis) from different sources (roots from field-grown plants or from in vitro cultures, cells from ... [more ▼]

The ginsenoside content of different ginseng species (Panax ginseng, P. quinquefolium, and P. vietnamensis) from different sources (roots from field-grown plants or from in vitro cultures, cells from solid calluses or from liquid cultures, commercial powders, and suspensions) is evaluated by means of a new high-performance thin-layer chromatography (HPTLC) technique combining an automatic TLC sampler and scanner. The results are compared with those obtained through more classical gross spectrometric and high-performance liquid chromatography (HPLC) techniques. HPTLC and HPLC allow the separation and estimation of the different ginsenosides. For this, HPTLC is faster and simpler than HPLC. Both techniques determine less amounts of ginsenosides than spectrophotometry, which displays overestimated values caused by light absorption by contaminating osides. In vitro cultured cells and roots contain the same ginsenosides as those produced by their mother plants, although at quite lower levels. The culture media also accumulates ginsenosides. [less ▲]

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See detailXylanase Production By Penicillium Canescens 10-10c In Solid-State Fermentation
Bakri, Y.; Jacques, Philippe; Thonart, Philippe ULg

in Applied Biochemistry and Biotechnology (2003), 105

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See detailInfluence Of A New Axial Impeller On K(L)A And Xylanase Production By Penicillium Canescens 10-10c
Bakri, Yasser; Jacques, Philippe; Shi, Kui et al

in Applied Biochemistry and Biotechnology (2002), 98

The effects of a new axial impeller (HTPG4) on oxygen volumetric transfer coefficient, KLa, and xylanase production by Penicillium canescens 10-10c were studied and compared for dual-impeller systems, one ... [more ▼]

The effects of a new axial impeller (HTPG4) on oxygen volumetric transfer coefficient, KLa, and xylanase production by Penicillium canescens 10-10c were studied and compared for dual-impeller systems, one with one DT4 impeller below and one HTPG4 above (DT4-HTPG4) and one with two DT4 (DT4-DT4) impellers, in a 5-L bioreactor. The volumetric coefficient of oxygen transfer was measured in culture medium using a gassing-out method at different gassing rates and agitation speeds. We observed that the DT4-HTPG4 combination provided better KLa performance than the DT4-DT4 combination. The two combinations were also tested for their influence on xylanase production by a filamentous microorganism; P. canescens 10-10c. These experiments demonstrated that the DT4-HTPG4 combination impeller enhanced enzyme production up to 23% compared with the DT4-DT4 combination at an aeration rate of 1 vvm and an agitation speed of 600 rpm. The main cause for this difference is thought to be a higher shear stress generated by the DT4-DT4 combination, which damages the mycelium of P. canescens and decreases xylanase production. [less ▲]

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See detailDifferent techniques to study the conformation of surfactins at interfaces
Deleu, Magali ULg; Gallet, Xavier; Brasseur, Robert et al

Poster (2001)

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See detailDissemination of Catabolic Plasmids among Desiccation-Tolerant Bacteria in Soil Microcosms
Weekers, Frederic; Rodriguez, Christian; Jacques, Philippe et al

in Applied Biochemistry and Biotechnology (2001), 91-93(Spring), 219-32

The dissemination of catabolic plasmids was compared to bioaugmentation by strain inoculation in microcosm experiments. When Rhodococcus erythropolis strain T902, bearing a plasmid with trichloroethene ... [more ▼]

The dissemination of catabolic plasmids was compared to bioaugmentation by strain inoculation in microcosm experiments. When Rhodococcus erythropolis strain T902, bearing a plasmid with trichloroethene and isopropylbenzene degradation pathways, was used as the inoculum, no transconjugant was isolated but the strain remained in the soil. This plasmid had a narrow host range. Pseudomonas putida strain C8S3 was used as the inoculum in a second approach. It bore a broad host range conjugative plasmid harboring a natural transposon, RP4::Tn4371, responsible for biphenyl and 4-chlorobiphenyl degradation pathways. The inoculating population slowly decreased from its original level (10(6) colony-forming units [CFU]/g of dry soil) to approx 3 x 10(2) CFU/g of dry soil after 3 wk. Transconjugant populations degrading biphenyl appeared in constant humidity soil (up to 2 x 10(3) CFU/g) and desiccating soil (up to 10(4) CFU/g). The feasibility of plasmid dissemination as a bioaugmentation technique was demonstrated in desiccating soils. The ecologic significance of desiccation in bioaugmentation was demonstrated: it upset the microbial ecology and the development of transconjugants. [less ▲]

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See detailConformational analysis of surfactins at interfaces
Deleu, Magali ULg; Gallet, Xavier; Dufrêne, Yves et al

Poster (2000)

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See detailRevue bibliographique : l’hydroperoxyde lyase
Delcarte, Jérome; Fauconnier, Marie-Laure ULg; Hoyaux, Phryné et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2000), 4(3), 157-167

The hydroperoxide lyase belongs to the lipoxygenase pathway, pathway in which polyunsaturated fatty acids are transformed into a large spectrum of compounds with various chemical and biological functions ... [more ▼]

The hydroperoxide lyase belongs to the lipoxygenase pathway, pathway in which polyunsaturated fatty acids are transformed into a large spectrum of compounds with various chemical and biological functions. The hydroperoxide lyase acts on fatty acids hydroperoxides (mainly linoleic and linolenic). It cleaves the bond between carbon carrying the hydroperoxide function and a vicinal carbon giving an w-oxo-acid and also depending on the species, an aldehyde, a hydrocarbon or an alcohol. [less ▲]

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See detailBiological treatment of waste gypsum
Hiligsmann, Serge ULg; Jacques, Philippe; Thonart, Philippe ULg

in Biodegradation (1998)

The presentation presents the investigations about gypsum treatment by sulfate-reducing bacteria

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See detailStructural analysis of two pyoverdins by electrospray and FAB mass spectrometry
Ongena, MARC ULg; Jacques, Philippe; van Vyncht, Gery et al

in Journal of the Mass Spectrometry Society of Japan (1998), 48

Pyoverdins are chromopeptides produced by the so-called fluorescent group of the bacterial genus Pseudomonas. Species or even strain specific they differ in the composition of their peptide chains. For ... [more ▼]

Pyoverdins are chromopeptides produced by the so-called fluorescent group of the bacterial genus Pseudomonas. Species or even strain specific they differ in the composition of their peptide chains. For classification purposes it is of importance to get fast information regarding the nature of the latter. For two new representatives from hydrolysis results and especially from an analysis of the data obtained from electrospray ionization (ESI) and fast atom bombardement (fAn) mass spectrometry measurements the amino acid sequences can be suggested as Asp-BuOHOrn-Dab-aThr-Gly-Ser-Ser-OHAsp-aThr (Pseudomonas putida BTP16) and Ser-SerFoOHOrn-Ser-Ser-Ser-FoOHOrn-Lys-Lys (Pseudomolnas fluorescens BTP7). [less ▲]

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See detailFoaming Properties Of Surfactin, A Lipopeptide Biosurfactant From Bacillus Subtilis
Razafindralambo, Hary ULg; Paquot, Michel ULg; Baniel, Alain et al

in Journal of the American Oil Chemists' Society (1996), 73(1), 149-151

Foaming properties of surfactin were investigated and compared to those of sodium dodecyl sulfate (SDS) and bovine serum albumin (BSA). Foams were formed by a bubbling technique. Evolution of the foam ... [more ▼]

Foaming properties of surfactin were investigated and compared to those of sodium dodecyl sulfate (SDS) and bovine serum albumin (BSA). Foams were formed by a bubbling technique. Evolution of the foam volume and the liquid in the foam was monitored with optical and conductimetric methods to characterize foam formation and stability. Excellent foaming properties of surfactin were shown by its higher ability to form and stabilize the foam at a concentration as low as 0.05 mg/mL, in comparison with SDS and BSA. Surfactin produced a foam with intermediate maximum density and stabilized the liquid in foam, as well as BSA. [less ▲]

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See detailImpact of the microbiology in the epuration processes.
Thonart, Philippe ULg; Destain, Jacqueline ULg; Antoine, Patrice et al

in Cerevisia and Biotechnology (1996), 21(2), 76-79

Biological treatments will progressively play a key role in the epuration process. Indeed, they show different advantages like reaction specificity, multiple degradation potentialities and efficiency at ... [more ▼]

Biological treatments will progressively play a key role in the epuration process. Indeed, they show different advantages like reaction specificity, multiple degradation potentialities and efficiency at very low concentrations of pollutants. Two approaches can be considered for developing biological processes of epuration : addition of bioactivators for improving endogenous microorganisms capabilities or introduction of microorganism starters for accelerating the epuration process. These two ways need as prerequisite a basic knowledge of the metabolic pathways for specific compound degradation and of microbial ecology [less ▲]

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See detailInfluence of lipopeptide production on oxygen transfer during fermentation of Bacillus subtilis
Hbid, Choukri; Jacques, Philippe; Razafindralambo, Hary ULg et al

Poster (1995, May 07)

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See detailLes mécanismes biochimiques développés par les Pseudomonas fluorescents dans la lutte biologique contre les maladies des plantes transmises par le sol
Jacques, Philippe; Delfosse, Philippe; Ongena, MARC ULg et al

in Cahiers Agricultures (1993), 2

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See detailThe Enterococcus Hirae R40 Penicillin-Binding Protein 5 and the Methicillin-Resistant Staphylococcus Aureus Penicillin-Binding Protein 2' Are Similar
el Kharroubi, Aboubaker; Jacques, Philippe; Piras, Graziella et al

in Biochemical Journal (1991), 280(Pt 2), 463-469

The penicillin-resistant Enterococcus hirae R40 has a typical profile of membrane-bound penicillin-binding proteins (PBPs) except that the 71 kDa PBP5 of low penicillin affinity represents about 50% of ... [more ▼]

The penicillin-resistant Enterococcus hirae R40 has a typical profile of membrane-bound penicillin-binding proteins (PBPs) except that the 71 kDa PBP5 of low penicillin affinity represents about 50% of all the PBPs present. Water-soluble tryptic-digest peptides were selectively produced from PBP5, their N-terminal regions were sequenced and synthetic oligonucleotides were used as primers to generate a 476 bp DNA fragment by polymerase chain reaction. On the basis of these data, the PBP5-encoding gene was cloned in Escherichia coli by using pBR322 as vector. The gene, included in a 7.1 kb insert, had the information for a 678-amino acid-residue protein. PBP5 shows similarity, in the primary structure, with the high-molecular-mass PBPs of class B. In particular, amino acid alignment of the enterococcal PBP5 and the methicillin-resistant staphylococcal PBP2' generates scores that are 30, for the N-terminal domains, and 53, for the C-terminal domains, standard deviations above that expected for a run of 20 randomized pairs of proteins having the same amino acid compositions as the two proteins under consideration. [less ▲]

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See detailMode of Membrane Insertion and Sequence of a 32-Amino Acid Peptide Stretch of the Penicillin-Binding Protein 4 of Enterococcus Hirae
Jacques, Philippe; el Kharroubi, Aboubaker; Van Beeumen, Jozef et al

in FEMS Microbiology Letters (1991), 66(2), 119-123

Analysis of water-soluble derivatives of the Enterococcus hirae 75-kDa membrane-bound penicillin-binding protein 4 (PBP4) has yielded the amino acid sequence of a 32-amino acid polypeptide stretch. This ... [more ▼]

Analysis of water-soluble derivatives of the Enterococcus hirae 75-kDa membrane-bound penicillin-binding protein 4 (PBP4) has yielded the amino acid sequence of a 32-amino acid polypeptide stretch. This peptide is similar to peptide segments known to occur in the N-terminal domain of high-Mr PBPs of class B. The E. hirae PBP4 probably belongs to the same class. It is anchored in the membrane at the N-terminus of the polypeptide chain. [less ▲]

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See detailActive-Site and Membrane Topology of the Dd-Peptidase/Penicillin-Binding Protein No. 6 of Enterococcus Hirae (Streptococcus Faecium) A.T.C.C. 9790
El Kharroubi, Aboubaker; Piras, Graziella; Jacques, Philippe et al

in Biochemical Journal (1989), 262(2), 457-462

The membrane-bound 43,000-Mr penicillin-binding protein no. 6 (PBP6) of Enterococcus hirae consists of a 30,000-Mr DD-peptidase/penicillin-binding domain and a approximately 130-residue C-terminal ... [more ▼]

The membrane-bound 43,000-Mr penicillin-binding protein no. 6 (PBP6) of Enterococcus hirae consists of a 30,000-Mr DD-peptidase/penicillin-binding domain and a approximately 130-residue C-terminal appendage. Removal of this appendage by trypsin proteolysis has no marked effect on the catalytic activity and penicillin-binding capacity of the PBP. Anchorage of the PBP in the membrane appears to be mediated by a short 15-20-residue stretch at the C-terminal end of the appendage. The sequence of the 50-residue N-terminal region of the PBP shows high degree of homology with the sequences of the corresponding regions of the PBPs5 of Escherichia coli and Bacillus subtilis. On this basis the active-site serine residue occurs at position 35 in the enterococcal PBP. [less ▲]

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See detailCharacterization of the Trypsin-Solubilized Penicillin-Binding Proteins of Enterococcus hirae (Streptococcus faecium)
El Kharroubi, Aboubaker; Jacques, Philippe; Piras, Graziella et al

in Actor, Paul; Daneo-Moore, Lolita; Higgins, Michael L. (Eds.) et al Antibiotic Inhibition of Bacterial Cell Surface Assembly and Function (1988)

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See detailPurification and Characterization of a β-lactam-Resistant Penicillin-Binding Protein from Enterococcus hirae (Streptococcus faecium)
Jacques, Philippe; El Kharroubi, Aboubaker; Joris, Bernard ULg et al

in Actor, Paul; Daneo-Moore, Lolita; Higgins, Michael L. (Eds.) et al Antibiotic Inhibition of Bacterial Cell Surface Assembly and Function (1988)

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See detailPrimary structure of the Streptomyces R61 extracellular DD-peptidase. 2. Amino acid sequence data
Joris, Bernard ULg; Jacques, Philippe; Frère, Jean-Marie ULg et al

in European Journal of Biochemistry (1987), 162(3), 519-524

In order to confirm the Streptomyces codon usage, the Streptomyces R61 DD-peptidase was fragmented by cyanogen bromide cleavage of the carboxymethylated protein, trypsin digestion of the carboxymethylated ... [more ▼]

In order to confirm the Streptomyces codon usage, the Streptomyces R61 DD-peptidase was fragmented by cyanogen bromide cleavage of the carboxymethylated protein, trypsin digestion of the carboxymethylated protein and trypsin digestion of the protein treated with beta-iodopenicillinate and endoxo-delta 4-tetrahydrophthalic acid. The isolated peptides, which altogether represented more than 50% of the polypeptide chain, were sequenced. The data thus obtained were in excellent agreement with the primary structure of the protein as deduced from the nucleotide sequence of the cloned gene. Though a weak acylating agent, beta-iodopenicillanate reacted selectively with the active site of the DD-peptidase and formed an adduct which mas much more stable than that formed with benzylpenicillin, thus facilitating the isolation and characterization of the active-site peptide. [less ▲]

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