References of "Jérôme, Christine"
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See detailpH-sensitive micellar systems for controlled drug delivery: synthesis and structural characterization by small-angle neutron scattering
Joset, Arnaud ULg; Jérôme, Christine ULg; Brulet, Annie et al

Poster (2009, March)

The aim of the project is the preparation of micellar nanocarriers made of biocompatibles copolymers and their structural analysis by Small Angle Neutron Scattering (SANS). These micelles could be used in ... [more ▼]

The aim of the project is the preparation of micellar nanocarriers made of biocompatibles copolymers and their structural analysis by Small Angle Neutron Scattering (SANS). These micelles could be used in drug delivery applications to fight cancer1. The hydrophobic polycaprolactone (PCL) core is intended to incorporate the drug. The corona of hydrophilic polyethylene oxide (PEO) stabilizes the nanocarriers with respect to the plasma proteins. The pH in the neighborhood of the tumoral cells is lower than in the healthy cells. We incorporated a pH-sensitive sequence of poly(2-vinylpyridine) (P2VP). As a result, these micelles are expected to deliver their drug near the cancerous cells without affecting the healthy cells. When the pH is acidic, the P2VP is protonated and the chains are repulsive. The micellar size is then larger than in basic pH, when the P2VP is precipitated on the PCL core. We prepared PCL65-b-P2VP31 / PCL65-b-PEO114 and PCL32-b-P2VP52 / PCL36-b-PEO114 50:50 mixtures of diblocks copolymers. The resulting mixed micelles are analyzed by SANS. We developed a theoretical model with a spherical water-free PCL core. The PEO corona is described as consisting of gaussian chains with a thickness estimated as twice the chain gyration radius, Rg. We propose two alternatives for handling the P2VP zone. The P2VP molecules are either assumed to be Gaussian chains or they fill a shell of thickness L with possible water penetration.The fitting of the models to the experimental scattering cross sections leads to important structural parameters like the aggregation number, the core radius, the gyration radius and the thickness of the P2VP shell. [less ▲]

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See detailRelevance of a prereaction for the in situ NMP of styrene using the C-phenyl-N-tert-butylnitrone/2,2’-azobis(isobutyronitrile) pair
Sciannamea, Valérie; Catala, Jean-Marie; Jérôme, Robert ULg et al

in Journal of Polymer Science. Part A, Polymer Chemistry (2009), 47(4), 1085-1097

In this article, we compare two routes for carrying out in situ nitroxide-mediated polymerization of styrene using the C-phenyl-N-tert-butylnitrone (PBN)/2,2-azobis(isobutyronitrile) (AIBN) pair to ... [more ▼]

In this article, we compare two routes for carrying out in situ nitroxide-mediated polymerization of styrene using the C-phenyl-N-tert-butylnitrone (PBN)/2,2-azobis(isobutyronitrile) (AIBN) pair to identify the best one for an optimal control. One route consists in adding PBN to the radical polymerization of styrene, while the other approach deals with a prereaction between the nitrone and the free radical initiator prior to the addition of the monomer and the polymerization. The combination of ESR and kinetics studies allowed demonstrating that when the polymerization of styrene is initiated by AIBN in the presence of enough PBN at 110 °C, fast decomposition of AIBN is responsible for the accumulation of dead polymer chains at the early stages of the polymerization, in combination with controlled polystyrene chains. On the other hand, PBN acts as a terminating agent at 70 °C with the formation of a polystyrene end-capped by an alkoxyamine, which is not labile at this temperature but that can be reactivated and chain-extended by increasing the temperature. Finally, the radical polymerization of styrene is better controlled when the nitrone/initiator pair is prereacted at 85 °C for 4 h in toluene before styrene is added and polymerized at 110 °C. [less ▲]

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See detailIsoprene-assisted radical coupling of (co)polymers prepared by cobalt-mediated radical polymerization
Debuigne, Antoine ULg; Jérôme, Christine ULg; Detrembleur, Christophe ULg

in Angewandte Chemie (International ed. in English) (2009), 48(8), 1422-1424

The isoprene-assisted radical coupling (I-ARC) of polymers prepared by cobalt-mediated radical polymerization (see picture) is the first efficient radical coupling method that is not restricted to short ... [more ▼]

The isoprene-assisted radical coupling (I-ARC) of polymers prepared by cobalt-mediated radical polymerization (see picture) is the first efficient radical coupling method that is not restricted to short chains. When applied to AB diblock copolymers, I-ARC constitutes a straightforward approach to the preparation of novel symmetrical ABA triblock copolymers [less ▲]

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See detailElectrografting method for forming and regulating a strong adherent nanostructured polymer coating
Voccia, Samuel; Serwas, Harry ULg; Jérôme, Christine ULg et al

Patent (2009)

Electrografting method for forming and regulating a strongly adherent nanostructured polymer coating onto an electro-conductive surface profile characterized in that the surface profile is regulated by ... [more ▼]

Electrografting method for forming and regulating a strongly adherent nanostructured polymer coating onto an electro-conductive surface profile characterized in that the surface profile is regulated by electrodeposition of nanometre- and/or micrometre-scale nuclei onto the surface profile prior to or simultaneously to the formation of the polymer coating. [less ▲]

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See detailStainless steel grafting of hyperbranched polymer brushes with an antibacterial activity: synthesis, characterization and properties
Ignatova, Milena; Voccia, Samuel; Gabriel, Sabine et al

in Langmuir (2009), 25(2), 891-902

Two strategies were used for the preparation of hyperbranched polymer brushes with a high density of functional groups: (a) the cathodic electrografting of stainless steel by poly[2-(2-chloropropionate ... [more ▼]

Two strategies were used for the preparation of hyperbranched polymer brushes with a high density of functional groups: (a) the cathodic electrografting of stainless steel by poly[2-(2-chloropropionate)ethyl acrylate] [poly(cPEA)], which was used as a macroinitiator for the atom transfer radical polymerization of an inimer, 2-(2-bromopropionate)ethyl acrylate in the presence or absence of heptadecafluorodecyl acrylate, (b) the grafting of preformed hyperbranched poly(ethyleneimine) onto poly(N-succinimidyl acrylate) previously electrografted onto stainless steel. The hyperbranched polymer, which contained either bromides or amines, was quaternized because the accordingly formed quaternary ammonium or pyridinium groups are known for antibacterial properties. The structure, chemical composition, and morphology of the quaternized and nonquaternized hyperbranched polymer brushes were characterized by ATR-FTIR reflectance, Raman spectroscopy, X-ray photoelectron spectroscopy, and atomic force microscopy. The peeling test confirmed that the grafted hyperbranched polymer films adhered much more strongly to stainless steel than the nongrafted solvent-cast films. The quaternized hyperbranched polymer brushes were more effective in preventing both protein adsorption and bacterial adhesion than quaternary ammonium containing poly(cPEA) primary films, more likely because of the higher hydrophilicity and density of cationic groups. [less ▲]

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See detailSynthesis and pH-dependent micellization of diblock copolymer mixtures
Van Butsele, Kathy ULg; Sibret, Pierre ULg; Fustin, Charles-André et al

in Journal of Colloid & Interface Science (2009), 329(2), 235-243

This work focused on the preparation and the aqueous solution properties of hybrid polymeric micelles consisting of a hydrophobic poly(ε-caprolactone) (PCL) core and a mixed shell of hydrophilic poly ... [more ▼]

This work focused on the preparation and the aqueous solution properties of hybrid polymeric micelles consisting of a hydrophobic poly(ε-caprolactone) (PCL) core and a mixed shell of hydrophilic poly(ethylene oxide) (PEO) and pH-sensitive poly(2-vinylpyridine) (P2VP). The hybrid micelles were successfully prepared by the rapid addition of acidic water to a binary solution of PCL34-b-PEO114 and PCL32-b-P2VP52 diblock copolymers in N,N-dimethylformamide. These micelles were pH-responsive as result of the pH-dependent ionization of the P2VP block. The impact of pH on the self-assembly of the binary mixture of diblocks—thus on the composition, shape, size and surface properties of the micelles—was studied by a variety of experimental techniques, i.e., dynamic and static light scattering, transmission electron microscopy, Zeta potential, fluorescence spectroscopy and complement hemolytic 50 test. [less ▲]

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See detailSelf-assembly and pH-responsiveness of ABC miktoarm star terpolymers
Van Butsele, Kathy ULg; Fustin, Charles-André; Gohy, Jean-François et al

in Langmuir (2009), 25(1), 107-111

This work deals with the self-assembly in water of ABC miktoarm star terpolymers consisting of hydrophobic poly(-caprolactone), hydrophilic poly(ethylene oxide) (PEO), and pH-sensitive poly(2 ... [more ▼]

This work deals with the self-assembly in water of ABC miktoarm star terpolymers consisting of hydrophobic poly(-caprolactone), hydrophilic poly(ethylene oxide) (PEO), and pH-sensitive poly(2-vinylpyridine) (P2VP). A variety of experimental techniques were used, including dynamic light scattering, transmission electron microscopy, and zeta potential. Special attention was paid to the pH dependency of the supramolecular self-assemblies. A key observation is the capability of the miktoarm terpolymers to form micelles stable over the whole range of pH, although a transition was observed from neutral to highly positively charged nanoobjects upon decreasing pH. [less ▲]

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See detailPaclitaxel-loaded PEGylated PLGA-based nanoparticles: in vitro and in vivo evaluation
Danhier, Fabienne; Lecouturier, Nathalie; Vroman, Benoît et al

in Journal of Controlled Release (2009), 133(1), 11-17

The incorporation efficiency of PTX was higher with the nanoprecipitation technique. The release behavior of PTX exhibited a biphasic pattern characterized by an initial burst release followed by a slower ... [more ▼]

The incorporation efficiency of PTX was higher with the nanoprecipitation technique. The release behavior of PTX exhibited a biphasic pattern characterized by an initial burst release followed by a slower and continuous release. The in vitro anti-tumoral activity was assessed using the Human Cervix Carcinoma cells (HeLa) by the MTT test and was compared to the commercial formulation Taxol® and to Cremophor® EL. When exposed to 25 µg/ml of PTX, the cell viability was lower for PTX-loaded nanoparticles than for Taxol® (IC50 5.5 vs 15.5 µg/ml). Flow cytometry studies showed that the cellular uptake of PTX-loaded nanoparticles was concentration and time dependent. Exposure of HeLa cells to Taxol® and PTX-loaded nanoparticles induced the same percentage of apoptotic cells. PTX-loaded nanoparticles showed greater tumor growth inhibition effect in vivo on TLT tumor, compared with Taxol®. Therefore, PTX-loaded nanoparticles may be considered as an effective anticancer drug delivery system for cancer chemotherapy. [less ▲]

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See detailPatenting activity in manufacturing organoclays for nanocomposite applications
Naveau, Elodie ULg; Detrembleur, Christophe ULg; Jérôme, Christine ULg et al

in Recent Patents on Materials Science (2009), 2(1), 43-49

For the last two decades, intensive research has been focused on developing reinforced polymers with incorporation of nanometric fillers. Amongst the different types of nanofillers, those based on layered ... [more ▼]

For the last two decades, intensive research has been focused on developing reinforced polymers with incorporation of nanometric fillers. Amongst the different types of nanofillers, those based on layered silicates (commonly known as clays), have been most widely investigated. Dispersing clay sheets on a nanoscopic scale (so-called exfoliation) indeed allows materials with enhanced thermal, mechanical, rheological, flame retardancy and barrier properties to be produced. However, the nanocomposite performances are strongly dependent upon the extent of clay exfoliation. In order to enhance the compatibility between the pristine clay, hydrophilic, and the polymer, hydrophobic, and to achieve a good delamination of the nanolayers, an organo-modification of the clay is most usually necessary. This mini-review will provide an outline of patenting activity in the field of manufacturing organoclays through ionic exchange. The variety of organic modifiers and the diverse processing techniques will be detailed, aiming to extract the most relevant organoclays for successful nanocomposite formation at industrial scale. [less ▲]

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See detailDevelopment of a procedure to simultaneously isolate RNA, DNA, and proteins from characterizing cells invading or cultured on chitosan scaffolds.
Tchemtchoua Tateu, Victor ULg; Atanasova, Ganka; Aqil, Abdelhafid ULg et al

in Analytical Biochemistry (2009), 393(1), 145-7

For many years, chitosan and its derivatives have been considered to be promising biomaterials for tissue engineering and repair. However, information regarding their biological effect on cell phenotype ... [more ▼]

For many years, chitosan and its derivatives have been considered to be promising biomaterials for tissue engineering and repair. However, information regarding their biological effect on cell phenotype is usually limited to evaluation of cell proliferation and survival, overlooking proteomic and transcriptomic analysis. This is largely related to the lack of efficient and quantitative procedures for protein and nucleic acid purification from cells cultured on, or inside, chitosan scaffold. Here we describe an ultracentrifugation procedure enabling the simultaneous and quantitative recovery of high quality RNA, DNA and proteins from cells growing in close contact of biomaterial matrices containing chitosan. [less ▲]

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See detailThermally-responsive nanogels by RAFT-mediated aqueous dispersion polymerization
Rieger, Jutta ULg; Grazon, Chloé; Bui, Chuong et al

in PMSE preprints (2009), 101

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See detailTargeting nanoparticles to M cells with non-peptidic ligands for oral vaccination
Fievez, Virginie; Plapied, Laurence; des Rieux, Anne et al

in European Journal of Pharmaceutics & Biopharmaceutics (2009)

The presence of RGD on nanoparticles allows the targeting of β1 integrins at the apical surface of human M cells and the enhancement of an immune response after oral immunization. To check the hypothesis ... [more ▼]

The presence of RGD on nanoparticles allows the targeting of β1 integrins at the apical surface of human M cells and the enhancement of an immune response after oral immunization. To check the hypothesis that non-peptidic ligands targeting intestinal M cells or APCs would be more efficient for oral immunization than RGD, novel non-peptidic and peptidic analogs (RGD peptidomimitic (RGDp), LDV derivative (LDVd) and LDV peptidomimetic (LDVp)) as well as mannose were grafted on the PEG chain of PCL–PEG and incorporated in PLGA-based nanoparticles. RGD and RGDp significantly increased the transport of nanoparticles across an in vitro model of human M cells as compared to enterocytes. RGD, LDVp, LDVd and mannose enhanced nanoparticle uptake by macrophages in vitro. The intraduodenal immunization with RGDp-, LDVd- or mannose-labeled nanoparticles elicited a higher production of IgG antibodies than the intramuscular injection of free ovalbumin or intraduodenal administration of either non-targeted or RGD-nanoparticles. Targeted formulations were also able to induce a cellular immune response. In conclusion, the in vitro transport of nanoparticles, uptake by macrophages and the immune response were positively influenced by the presence of ligands at the surface of nanoparticles. These targeted-nanoparticles could thus represent a promising delivery system for oral immunization. [less ▲]

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See detailFunctional amphiphilic and degradable copolymers for drug delivery systems
Freichels, Hélène ULg; Pourcelle, Vincent; Plapied, Laurence et al

Poster (2008, December 18)

Detailed reference viewed: 23 (4 ULg)
See detailSummary of aim and achievements in Work Package 7 "Functional Coatings"
Jérôme, Christine ULg

Conference (2008, November 28)

Detailed reference viewed: 10 (2 ULg)
See detailDesign of thermoresponsive inorganic nanoparticles
Aqil, Abdelhafid ULg; Jérôme, Christine ULg

Conference (2008, November 28)

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See detailNew strategies to prepare melt processable polymer-grafted gold nanoparticles
Alexandre, Michaël ULg; Abetz, Volker; Boschetti-de-Fierro, A. et al

Poster (2008, November 28)

Detailed reference viewed: 31 (6 ULg)
See detailStimuli responsive nanogels for targeted drug delivery
Jérôme, Christine ULg

Conference (2008, November 28)

Detailed reference viewed: 10 (2 ULg)
See detailPolymer membrane by electrospinning
Aqil, Abdelhafid ULg; Grignard, Bruno ULg; Croisier, Florence ULg et al

Poster (2008, November 28)

Detailed reference viewed: 34 (8 ULg)
See detailElectrografting of thermo-responsive polymer films on conducting surfaces
Gabriel, Sabine; Stach, M.; Confortini, O. et al

Poster (2008, November 28)

Detailed reference viewed: 26 (2 ULg)