References of "Jérôme, Christine"
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See detailSynthesis and pH-dependent micellization of diblock copolymer mixtures
Van Butsele, Kathy ULg; Sibret, Pierre ULg; Fustin, Charles-André et al

in Journal of Colloid & Interface Science (2009), 329(2), 235-243

This work focused on the preparation and the aqueous solution properties of hybrid polymeric micelles consisting of a hydrophobic poly(ε-caprolactone) (PCL) core and a mixed shell of hydrophilic poly ... [more ▼]

This work focused on the preparation and the aqueous solution properties of hybrid polymeric micelles consisting of a hydrophobic poly(ε-caprolactone) (PCL) core and a mixed shell of hydrophilic poly(ethylene oxide) (PEO) and pH-sensitive poly(2-vinylpyridine) (P2VP). The hybrid micelles were successfully prepared by the rapid addition of acidic water to a binary solution of PCL34-b-PEO114 and PCL32-b-P2VP52 diblock copolymers in N,N-dimethylformamide. These micelles were pH-responsive as result of the pH-dependent ionization of the P2VP block. The impact of pH on the self-assembly of the binary mixture of diblocks—thus on the composition, shape, size and surface properties of the micelles—was studied by a variety of experimental techniques, i.e., dynamic and static light scattering, transmission electron microscopy, Zeta potential, fluorescence spectroscopy and complement hemolytic 50 test. [less ▲]

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See detailSelf-assembly and pH-responsiveness of ABC miktoarm star terpolymers
Van Butsele, Kathy ULg; Fustin, Charles-André; Gohy, Jean-François et al

in Langmuir (2009), 25(1), 107-111

This work deals with the self-assembly in water of ABC miktoarm star terpolymers consisting of hydrophobic poly(-caprolactone), hydrophilic poly(ethylene oxide) (PEO), and pH-sensitive poly(2 ... [more ▼]

This work deals with the self-assembly in water of ABC miktoarm star terpolymers consisting of hydrophobic poly(-caprolactone), hydrophilic poly(ethylene oxide) (PEO), and pH-sensitive poly(2-vinylpyridine) (P2VP). A variety of experimental techniques were used, including dynamic light scattering, transmission electron microscopy, and zeta potential. Special attention was paid to the pH dependency of the supramolecular self-assemblies. A key observation is the capability of the miktoarm terpolymers to form micelles stable over the whole range of pH, although a transition was observed from neutral to highly positively charged nanoobjects upon decreasing pH. [less ▲]

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See detailPaclitaxel-loaded PEGylated PLGA-based nanoparticles: in vitro and in vivo evaluation
Danhier, Fabienne; Lecouturier, Nathalie; Vroman, Benoît et al

in Journal of Controlled Release (2009), 133(1), 11-17

The incorporation efficiency of PTX was higher with the nanoprecipitation technique. The release behavior of PTX exhibited a biphasic pattern characterized by an initial burst release followed by a slower ... [more ▼]

The incorporation efficiency of PTX was higher with the nanoprecipitation technique. The release behavior of PTX exhibited a biphasic pattern characterized by an initial burst release followed by a slower and continuous release. The in vitro anti-tumoral activity was assessed using the Human Cervix Carcinoma cells (HeLa) by the MTT test and was compared to the commercial formulation Taxol® and to Cremophor® EL. When exposed to 25 µg/ml of PTX, the cell viability was lower for PTX-loaded nanoparticles than for Taxol® (IC50 5.5 vs 15.5 µg/ml). Flow cytometry studies showed that the cellular uptake of PTX-loaded nanoparticles was concentration and time dependent. Exposure of HeLa cells to Taxol® and PTX-loaded nanoparticles induced the same percentage of apoptotic cells. PTX-loaded nanoparticles showed greater tumor growth inhibition effect in vivo on TLT tumor, compared with Taxol®. Therefore, PTX-loaded nanoparticles may be considered as an effective anticancer drug delivery system for cancer chemotherapy. [less ▲]

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See detailPatenting activity in manufacturing organoclays for nanocomposite applications
Naveau, Elodie ULg; Detrembleur, Christophe ULg; Jérôme, Christine ULg et al

in Recent Patents on Materials Science (2009), 2(1), 43-49

For the last two decades, intensive research has been focused on developing reinforced polymers with incorporation of nanometric fillers. Amongst the different types of nanofillers, those based on layered ... [more ▼]

For the last two decades, intensive research has been focused on developing reinforced polymers with incorporation of nanometric fillers. Amongst the different types of nanofillers, those based on layered silicates (commonly known as clays), have been most widely investigated. Dispersing clay sheets on a nanoscopic scale (so-called exfoliation) indeed allows materials with enhanced thermal, mechanical, rheological, flame retardancy and barrier properties to be produced. However, the nanocomposite performances are strongly dependent upon the extent of clay exfoliation. In order to enhance the compatibility between the pristine clay, hydrophilic, and the polymer, hydrophobic, and to achieve a good delamination of the nanolayers, an organo-modification of the clay is most usually necessary. This mini-review will provide an outline of patenting activity in the field of manufacturing organoclays through ionic exchange. The variety of organic modifiers and the diverse processing techniques will be detailed, aiming to extract the most relevant organoclays for successful nanocomposite formation at industrial scale. [less ▲]

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See detailDevelopment of a procedure to simultaneously isolate RNA, DNA, and proteins from characterizing cells invading or cultured on chitosan scaffolds.
Tchemtchoua Tateu, Victor ULg; Atanasova, Ganka; Aqil, Abdelhafid ULg et al

in Analytical Biochemistry (2009), 393(1), 145-7

For many years, chitosan and its derivatives have been considered to be promising biomaterials for tissue engineering and repair. However, information regarding their biological effect on cell phenotype ... [more ▼]

For many years, chitosan and its derivatives have been considered to be promising biomaterials for tissue engineering and repair. However, information regarding their biological effect on cell phenotype is usually limited to evaluation of cell proliferation and survival, overlooking proteomic and transcriptomic analysis. This is largely related to the lack of efficient and quantitative procedures for protein and nucleic acid purification from cells cultured on, or inside, chitosan scaffold. Here we describe an ultracentrifugation procedure enabling the simultaneous and quantitative recovery of high quality RNA, DNA and proteins from cells growing in close contact of biomaterial matrices containing chitosan. [less ▲]

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See detailThermally-responsive nanogels by RAFT-mediated aqueous dispersion polymerization
Rieger, Jutta ULg; Grazon, Chloé; Bui, Chuong et al

in PMSE preprints (2009), 101

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See detailTargeting nanoparticles to M cells with non-peptidic ligands for oral vaccination
Fievez, Virginie; Plapied, Laurence; des Rieux, Anne et al

in European Journal of Pharmaceutics & Biopharmaceutics (2009)

The presence of RGD on nanoparticles allows the targeting of β1 integrins at the apical surface of human M cells and the enhancement of an immune response after oral immunization. To check the hypothesis ... [more ▼]

The presence of RGD on nanoparticles allows the targeting of β1 integrins at the apical surface of human M cells and the enhancement of an immune response after oral immunization. To check the hypothesis that non-peptidic ligands targeting intestinal M cells or APCs would be more efficient for oral immunization than RGD, novel non-peptidic and peptidic analogs (RGD peptidomimitic (RGDp), LDV derivative (LDVd) and LDV peptidomimetic (LDVp)) as well as mannose were grafted on the PEG chain of PCL–PEG and incorporated in PLGA-based nanoparticles. RGD and RGDp significantly increased the transport of nanoparticles across an in vitro model of human M cells as compared to enterocytes. RGD, LDVp, LDVd and mannose enhanced nanoparticle uptake by macrophages in vitro. The intraduodenal immunization with RGDp-, LDVd- or mannose-labeled nanoparticles elicited a higher production of IgG antibodies than the intramuscular injection of free ovalbumin or intraduodenal administration of either non-targeted or RGD-nanoparticles. Targeted formulations were also able to induce a cellular immune response. In conclusion, the in vitro transport of nanoparticles, uptake by macrophages and the immune response were positively influenced by the presence of ligands at the surface of nanoparticles. These targeted-nanoparticles could thus represent a promising delivery system for oral immunization. [less ▲]

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See detailFunctional amphiphilic and degradable copolymers for drug delivery systems
Freichels, Hélène ULg; Pourcelle, Vincent; Plapied, Laurence et al

Poster (2008, December 18)

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See detailSummary of aim and achievements in Work Package 7 "Functional Coatings"
Jérôme, Christine ULg

Conference (2008, November 28)

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See detailDesign of thermoresponsive inorganic nanoparticles
Aqil, Abdelhafid ULg; Jérôme, Christine ULg

Conference (2008, November 28)

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See detailNew strategies to prepare melt processable polymer-grafted gold nanoparticles
Alexandre, Michaël ULg; Abetz, Volker; Boschetti-de-Fierro, A. et al

Poster (2008, November 28)

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See detailStimuli responsive nanogels for targeted drug delivery
Jérôme, Christine ULg

Conference (2008, November 28)

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See detailPolymer membrane by electrospinning
Aqil, Abdelhafid ULg; Grignard, Bruno ULg; Croisier, Florence ULg et al

Poster (2008, November 28)

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See detailElectrografting of thermo-responsive polymer films on conducting surfaces
Gabriel, Sabine; Stach, M.; Confortini, O. et al

Poster (2008, November 28)

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See detailPreparation and characterization of thermoresponsive iron nanoparticles for biomedical applications
Sibret, Pierre ULg; Aqil, Abdelhafid ULg; Gohy, Jean-François et al

Poster (2008, November 28)

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See detailDesign of perfluorinated macroligand for the implementation of atom transfer radical polymerization in supercritical carbon dioxide
Grignard, Bruno ULg; Jérôme, Christine ULg; Calberg, Cédric ULg et al

Conference (2008, November 28)

Due to an increasing need for polymers with well-defined architecture (diblock-, graft-, star-shaped copolymers), molecular weight and/or functional end-groups, the use of controlled radical ... [more ▼]

Due to an increasing need for polymers with well-defined architecture (diblock-, graft-, star-shaped copolymers), molecular weight and/or functional end-groups, the use of controlled radical polymerization (CRP) in scCO2 has started to gain attention. Among all the controlled processes, Atom Transfer Radical Polymerization has emerged as a robust tool for the preparation of polymers with well-defined molecular weight, architecture and chain-end functionality. The goal of the research relies on the development of ATRP of vinyl monomers in scCO2. Perfluorinated polymethacrylate, i.e. poly2,2,2-trifluoroethyl methacrylate (PFMA), was successfully prepared by homogenous ATRP using a polymeric ligand in order to complex the copper catalyst. CO2-soluble poly(2,2,2-trifluoroethylmethacrylate) was also prepared in supercritical CO2 by supported ATRP using a “pseudo-homogeneous” catalyst consisting of copper (I) ligated by macroligand immobilized onto an inorganic support, that results in polymers with well defined molecular weight and low polydispersity. The first efficient dispersion ATRP of methyl methacrylate (MMA) in scCO2 using a fluorinated polymeric ligand that had a dual role, i.e., the complexation of the copper salt and the stabilization of PMMA growing particles was also reported and the concept of dispersion ATRP was extended to the dispersion ATRP of styrene, to the synthesis of diblock copolymers beads using PMMA beads as macroinitiators, leading to (co)polymers with predictable molecular weight and narrow polydispersity. Finally, because both ATRP and alkyne-azide Huisgen’s 1,3-dipolar cycloaddition relies on the use of a Cu(I) catalyst, synthesis of pyrene end-functionalized polymers by simultaneous dispersion ATRP and click reaction was also investigated in supercritical carbon dioxide, leading to the formation of PMMA of well defined characteristics that was collected as fluorescent micropheres. [less ▲]

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See detailDispersion Atom Transfer Radical Polymerization of vinyl monomers in supercritical carbon dioxide
Grignard, Bruno ULg; Jérôme, Christine ULg; Calberg, Cédric ULg et al

in Macromolecules (2008), 41(22), 8575-8563

Controlled dispersion atom transfer radical polymerization (ATRP) of methyl methacrylate (MMA) was successfully carried out in supercritical carbon dioxide in the presence of aminated fluoropolymers ... [more ▼]

Controlled dispersion atom transfer radical polymerization (ATRP) of methyl methacrylate (MMA) was successfully carried out in supercritical carbon dioxide in the presence of aminated fluoropolymers. These materials played the dual role of macroligand for the copper bromide and also steric stabilizer to support formation of polymer microspheres. The livingness of the PMMA beads was confirmed by the one-pot two-step PMMA chain extension and the synthesis of poly(methyl methacrylate)-b-poly(2,2,2-trifluoroethyl methacrylate) (PMMA-b-PFMA) diblock copolymer in scCO2. Successful activator generated by electron transfer (AGET) for ATRP of MMA, using tin ethylhexanoate as a reducing agent, is also discussed, and the concept of dispersion ATRP of MMA was successfully extended to the controlled dispersion polymerization of styrene by ATRP leading to the formation of PS microparticles. Finally, due to the high solubility of the catalyst in scCO2, the purification of PMMA was investigated by supercritical fluid extraction, leading to the preparation of PMMA beads with low residual catalyst traces. [less ▲]

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See detailSynthesis of biodegradable poly-epsilon-caprolactone microspheres by dispersion ring-opening polymerization in supercritical carbon dioxide
Grignard, Bruno ULg; Stassin, Fabrice; Calberg, Cédric ULg et al

in Biomacromolecules (2008), 9(11), 3141-3149

A series of fluorinated diblock and triblock copolymers of poly(ε-caprolactone) and poly(heptadecafluorodecylacrylate) were prepared by combining ring-opening polymerization of ε-CL and atom transfer ... [more ▼]

A series of fluorinated diblock and triblock copolymers of poly(ε-caprolactone) and poly(heptadecafluorodecylacrylate) were prepared by combining ring-opening polymerization of ε-CL and atom transfer radical polymerization of the acrylate. These copolymers with well-controlled molecular weight and composition were characterized by 1H NMR spectroscopy and used as stabilizers for the dispersion ring-opening polymerization of ε-CL in supercritical carbon dioxide. The effect of composition and architecture of the polymeric stabilizers on the stabilization of PCL microspheres was investigated. Finally, purification of PCL was successfully implemented by reactive supercritical fluid extraction of the tin catalyst. [less ▲]

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