References of "Jérôme, Christine"
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See detailDrug delivery to inflamed colon by nanoparticles: comparison of different strategies
Coco, Régis; Plapied, Laurence; Pourcelle, Vincent et al

in International Journal of Pharmaceutics (2013), 440(1), 3-12

For inflammatory bowel disease (IBD) treatment, local delivery of molecules loaded in nanoparticles to the inflamed colon could be a promising strategy. The aim of this study was to investigate how drug ... [more ▼]

For inflammatory bowel disease (IBD) treatment, local delivery of molecules loaded in nanoparticles to the inflamed colon could be a promising strategy. The aim of this study was to investigate how drug-loaded polymeric nanoparticles target the site of inflammation and to analyse the influence of different colon-specific delivery strategies. Three different polymeric nanoparticles were formulated using ovalbumin (OVA) as a model drug. pH-sensitive nanoparticles were made with Eudragit® S100. Mucoadhesive nanoparticles were created with trimethylchitosan (TMC). A mix of polymers, PLGA, PEG-PLGA and PEG-PCL, were used to obtain a sustained drug delivery. Furthermore, ligands targeting immune cells (i.e. mannose) or the inflamed colon (i.e. a specific peptide) were grafted on the PEG chain of PCL. Interaction of nanoparticles with the intestinal epithelium was explored using Caco-2 monolayers designed to mimic an inflamed epithelium and then visualized using confocal laser microscopy. TMC nanoparticles had the highest apparent permeability for OVA in the untreated model. However, in the inflamed model, there were no difference between TMC, PLGA-based and Eudragit® nanoparticles. The uptake of nanoparticles in the inflamed mouse colon was assessed in a horizontal diffusion chamber. Mannose-grafted PLGA nanoparticles showed the highest accumulation of OVA in inflamed colon. Based on these results, active targeting of macrophages and dendritic cells may be a promising approach for targeting the colon in IBD. [less ▲]

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See detailCatechols as versatile platforms in polymer chemistry
Faure, Emilie; Falentin-Daudré, Céline; Jérôme, Christine ULg et al

in Progress in Polymer Science (2013), 38(1), 236-270

Catechols represent an important and versatile building block for the design of mussel-inspired synthetic adhesives and coatings. Indeed, their ability to establish large panoply of interactions with both ... [more ▼]

Catechols represent an important and versatile building block for the design of mussel-inspired synthetic adhesives and coatings. Indeed, their ability to establish large panoply of interactions with both organic and inorganic substrates has promoted catechol as a universal anchor for surface modifications. In addition to its pivotal role in adhesive interfaces, the catechol unit recently emerged as a powerful building block for the preparation of a large range of polymeric materials with intriguing structures and fascinating properties. The importance of catechols as efficient anchoring groups has been highlighted in recent excellent reviews partly dedicated to the characterization of their adhesive mechanisms onto surfaces and to their applications. The aim of this paper is to review for the first time the main synthetic approaches developed for the design of novel catechol-based polymer materials. We will also highlight the importance of these groups as versatile platforms for further functionalization of the macromolecular structures, but also surfaces. This will be illustrated by briefly discussing some advanced applications developed from these catechol-modified polymers. The review is organized according to the chemical structure of the functionalized catechol polymers. Chapter 1 discusses polymers bearing catechols embedded into the polymer main chain. Chapter 2 focuses on the attachment of catechol moieties as pendant groups and Chapter 3 describes the different approaches for incorporation of the catechol unit at the extremity of well-defined polymers. [less ▲]

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See detailPhotochemical properties and activity of water-soluble polymer/C60 nanohybrids for photodynamic therapy
Hurtgen, Marie ULg; Debuigne, Antoine ULg; Hoebeke, Maryse ULg et al

in Macromolecular Bioscience (2013), 13(1), 106-115

Water-soluble star-like poly(vinyl alcohol)/C60 and poly{[poly(ethylene glycol) acrylate]-co- (vinyl acetate)}/C60 nanohybrids are prepared by grafting macroradicals onto C60 and are assessed as ... [more ▼]

Water-soluble star-like poly(vinyl alcohol)/C60 and poly{[poly(ethylene glycol) acrylate]-co- (vinyl acetate)}/C60 nanohybrids are prepared by grafting macroradicals onto C60 and are assessed as photosensitizers for photodynamic therapy. The photophysical and biological properties of both nanohybrids highlight key characteristics influencing their overall efficiency. The macromolecular structure (linear/graft) and nature (presence/absence of hydroxyl groups) of the polymeric arms respectively impact the photodynamic activity and the stealthiness of the nanohybrids. The advantages of both nanohybrids are encountered in a third one, poly[(Nvinylpyrrolidone)- co-(vinyl acetate)]/C60, which has linear grafts without hydroxyl groups, and shows a better photodynamic activity. [less ▲]

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See detailEnzymatic synthesis and surface properties of novel rhamnolipids
Nott, Katherine ULg; Richard, Gaetan ULg; Laurent, Pascal ULg et al

in Process Biochemistry (2013), 48

New rhamnolipids were obtained via the development of a synthesis procedure consisting of two biocatalyzed steps. In the first step, naringinase was used to introduce a primary alcohol function onto ... [more ▼]

New rhamnolipids were obtained via the development of a synthesis procedure consisting of two biocatalyzed steps. In the first step, naringinase was used to introduce a primary alcohol function onto rhamnose by glycosylation of 1,3-propanediol. In the second step, immobilized lipase B from Candida antarctica catalyzed the esterification of the primary hydroxyl group with mono- and di-carboxylic fatty acids of increasing chain length (from C8 to C14). For the monoic acids, the initial rate and 24 h yield decreased with increasing chain length. For the dioic acid, the number of carbon atoms of the acid did not influence these parameters. The new rhamnolipid obtained with tetradecanoic acid showed very good surface properties. At pH 5, it had a very low critical aggregation concentration of 1.70 M and it diminished water’s surface tension to 27.6 mN/m. It was also able to form stable insoluble monolayers. On the other hand, the rhamnolipid formed with tetradecanedioic acid showed far less interesting surface properties. [less ▲]

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See detailNovel functional degradable block copolymers for the building of reactive micelles
Cajot, Sébastien; Lecomte, Philippe ULg; Jérôme, Christine ULg et al

in Polymer Chemistry (2013), 4(4), 1025-1037

Amphiphilic biocompatible copolymers are promising materials for the elaboration of nanosystems for drug delivery applications. This paper aims at reporting on the synthesis of new functional amphiphilic ... [more ▼]

Amphiphilic biocompatible copolymers are promising materials for the elaboration of nanosystems for drug delivery applications. This paper aims at reporting on the synthesis of new functional amphiphilic copolymers based on biocompatible and bioeliminable blocks. Poly(ethylene oxide) was selected as the hydrophilic block, whereas an aliphatic polyester, i.e. poly(epsilon-caprolactone), or a polycarbonate, i.e. poly(trimethylene carbonate), were chosen as the degradable hydrophobic block. In order to allow a post-functionalization of the micelles core, azide groups were introduced on the hydrophobic segment to provide reactivity towards functional alkyne derivatives by the copper azide-alkyne cycloaddition (CuAAC). For this purpose, a functional lactone, i.e. alpha-chloro-epsilon-caprolactone was introduced during the polymerization of the hydrophobic block before being converted into azide on the preformed copolymer. Such reactivity of the block copolymers and their self-assemblies is of prime interest for drugs or fluorescent dyes grafting, so as for micelles cross-linking. The influence of the azides distribution along the degradable block on the micelles post-functionalization ability has been studied by using alkyne bearing fluorescent dyes as model for drugs. The hydrophilicity of the dye on the micelles post-functionalization efficiency has also been investigated. [less ▲]

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See detailBiomechanical and optical properties of 2 new hydrophobic platforms for intraocular lenses
Bozukova, Dimitriya; Pagnoulle, Christophe; Jérôme, Christine ULg

in Journal of Cataract & Refractive Surgery (2013), 39(9), 1404-1414

Purpose: To compare the biomechanical and optical properties of 2 new hydrophobic platforms and a series of commercially available foldable intraocular lenses (IOLs). Results: With 1 exception, IOLs ... [more ▼]

Purpose: To compare the biomechanical and optical properties of 2 new hydrophobic platforms and a series of commercially available foldable intraocular lenses (IOLs). Results: With 1 exception, IOLs equilibrated in aqueous medium had a lower glass-transition temperature, higher deformability, lower injection forces, and complete recovery of their initial optical properties after injection. Typical hydrophobic acrylic dry-packaged IOLs required higher injection forces with high residual deformation and lost part of their initial optical quality after injection. Hydrophobic acrylic C-loop, double C-loop, and closed quadripod haptics applied optimum compression forces to the capsular bag with negligible optic axial displacement and tilt compared with plate haptics and poly(methyl methacrylate) haptics. Conclusions: The combination of the C-loop haptic and the bioadhesive glistening-free material, which absorbs a predetermined amount of water, allowed for a biomechanically stable IOL. The same material used in association with a double C-loop haptic design facilitated the perioperative manipulation and placement of the IOL in a smaller capsular bag without impairing the other biomechanical properties of a single C-loop design. [less ▲]

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See detailIn vitro investigations of smart drug delivery systems based on redox-sensitive cross-linked micelles
Cajot, Sébastien; Schol, D.; Dahnier, F. et al

in Macromolecular Symposia (2013), 13(12), 1661-1670

Redox-sensitive micelles are designed by using block copolymers of different architectures composed of a hydrophilic block of poly(ethylene oxide), and hydrophobic blocks of poly(ϵ-caprolactone) and poly ... [more ▼]

Redox-sensitive micelles are designed by using block copolymers of different architectures composed of a hydrophilic block of poly(ethylene oxide), and hydrophobic blocks of poly(ϵ-caprolactone) and poly(α-azide-ϵ-caprolactone). Stability of these micelles is insured in diluted media by cross-linking their core via the addition of a bifunctional cross-linker, while redox sensitivity is provided to these micelles by inserting a disulfide bridge in the cross-linker. The potential of these responsive micelles to be used as nanocarriers is studied in terms of cytotoxicity and cellular internalization. The release profiles are also investigated by varying the environment reductive strength. [less ▲]

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See detailReusability study of Novozym® 435 for the enzymatic synthesis of mannosyl myristate in pure ionic liquids
Galonde, Nadine ULg; Richard, Gaetan ULg; Deleu, Magali ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2013)

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See detailSynthesis and fabrication of polyesters as biomaterials
Lecomte, Philippe ULg; Jérôme, Christine ULg

in Popa, Valentin; Dumitriu, Daniela (Eds.) Polymeric biomaterials: structure and function, volume 1 (2013)

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See detailSynthesis of poly(vinyl acetate)-b-poly(vinyl chloride) block copolymers by cobalt-mediated radical polymerization (CMRP)
Piette, Yasmine; Debuigne, Antoine ULg; Bodart, Vinent et al

in Polymer Chemistry (2013), 4(5), 1685-1693

The synthesis of poly(vinyl acetate)-b-poly(vinyl chloride) (PVAc-b-PVC) block copolymers by Cobalt-Mediated Radical Polymerization (CMRP) is investigated for the first time in this paper. A PVAc–Co(acac ... [more ▼]

The synthesis of poly(vinyl acetate)-b-poly(vinyl chloride) (PVAc-b-PVC) block copolymers by Cobalt-Mediated Radical Polymerization (CMRP) is investigated for the first time in this paper. A PVAc–Co(acac)2 macroinitiator is prepared by CMRP using the V-70/Co(acac)2 binary system or a preformed alkylcobalt(III) compound. Then, the block copolymerization occurs in the bulk at 40 °C by the addition of VC. The addition of water to the polymerization medium or the slow generation of alkyl radicals during the whole polymerization is beneficial to the process by consuming part of the excess of deactivator (Co(acac)2) that blocks the polymer chains into the dormant form. Dynamic light scattering (DLS) measurements and AFM analyses evidence that the PVAc-b-PVC forms core–shell micelles in a selective solvent of the PVAc block, i.e. methanol, evidencing the blocky structure of the copolymer. PVAc-b-P(VC-co-VAc) copolymers are also successfully prepared by initiating the radical copolymerization of VC and VAc at 40 °C from a PVAc–Co(acac)2 macroinitiator. [less ▲]

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See detailFunctional nanogels as platforms for imparting antibacterial, antibiofilm, and antiadhesion activities to stainless steel
Faure, Emilie ULg; Falentin, Céline ULg; Svaldo Lanero, Tiziana ULg et al

in Advanced Functional Materials (2012), 22(24), 5271-5282

In this work, long-term antibacterial, antiadhesion, and antibiofilm activities are afforded to industrial stainless steel surfaces following a green and bio-inspired strategy. Starting from catechol ... [more ▼]

In this work, long-term antibacterial, antiadhesion, and antibiofilm activities are afforded to industrial stainless steel surfaces following a green and bio-inspired strategy. Starting from catechol bearing synthetic polymers, the film cross-linking and the grafting of active (bio)molecules are possible under environmentally friendly conditions (in aqueous media and at room temperature). A bio-inspired polyelectrolyte, a polycation-bearing catechol, is used as the film-anchoring polymer while a poly(methacrylamide)-bearing quinone groups serves as the cross-linking agent in combination with a polymer bearing primary amine groups. The amine/quinone reaction is exploited to prepare stable solutions of nanogels in water at room temperature that can be easily deposited to stainless steel. This coating provides quinonefunctionalized surfaces that are then used to covalently anchor active (bio) molecules (antibiofi lm enzyme and antiadhesion polymer) through thiol/ quinone reactions. [less ▲]

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See detailSmart nanocarriers for pH-triggered targeting and release of hydrophobic drugs
Cajot, Sébastien; Van Butsele, Kathy; Paillard, A. et al

in Acta Biomaterialia (2012), 8(12), 4215-4223

The use of hybrid pH-sensitive micelles mainly based on the PEO129-P2VP43-PCL17 ABC miktoarm star copolymer as potential triggered drug delivery systems has been investigated. Co-micellization of this ... [more ▼]

The use of hybrid pH-sensitive micelles mainly based on the PEO129-P2VP43-PCL17 ABC miktoarm star copolymer as potential triggered drug delivery systems has been investigated. Co-micellization of this star copolymer with a second copolymer labeled by a targeting ligand, i.e. biotin, on the pH sensitive block (poly-2-vinylpyridine, P2VP) has been considered here in order to impart possible active targeting of the tumor cells. Two architectures have been studied for these labeled copolymers, i.e. a miktoarm star or a linear ABC terpolymer and the respective hybrid micelles have been compared in terms of cytotoxicity (cells viability) and cellular uptake (by using fluorescent dye loaded micelles). Finally, the triggered drug release in the cytosol of tumor cells was investigated by studying on one hand the lysosomal integrity after internalization and on the other hand the release profile in function of the pH. [less ▲]

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See detailRing-opening polymerization of cyclic phosphoesters
Vanslambrouck, Stéphanie ULg; Clement, Benoît ULg; Jérôme, Christine ULg et al

Poster (2012, November 16)

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See detailSynthesis and physico-chemical characterization of fatty esters
Sainvitu, Pauline ULg; Nott, Katherine ULg; Nicks, Francois ULg et al

Poster (2012, November 16)

Specific antioxidant molecules (e.g. phenolics) help to prevent oxidation reaction of the cell membrane. A fatty chain grafted on these compounds should enhance their capacity to interact with the ... [more ▼]

Specific antioxidant molecules (e.g. phenolics) help to prevent oxidation reaction of the cell membrane. A fatty chain grafted on these compounds should enhance their capacity to interact with the membrane lipids. In our study, three fatty esters comprising an aromatic part were synthesized. They differentiate the aromatic substituent and the number of carbons between the aromatic ring and the ester function. A structure-function relationships study was performed to identify the structural pattern affecting the interfacial properties and the membrane interaction properties. The behavior of their monolayer film at an air-water interface was studied. The interactions with membrane were assessed on living cells and were predicted by a computational approach. In the future, we will investigate the effect of the presence of a sugar unit on these molecules. [less ▲]

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See detailSynthesis of new biomimetic and biodegradable polymers for clinical use
Clement, Benoît ULg; Grignard, Bruno ULg; Koole, Leo et al

Conference (2012, November 15)

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See detailSynthesis in scCO2 of nano-hydrogels for proteins delivery
Alaimo, David ULg; Grignard, Bruno ULg; Fustin, Charles-André et al

Conference (2012, November 14)

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