References of "Hubert, Philippe"
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See detailAutomated Determination of Verapamil and Norverapamil in Human Plasma with on-Line Coupling of Dialysis to High-Performance Liquid Chromatography and Fluorometric Detection
Ceccato, Attilio ULg; Chiap, Patrice ULg; Hubert, Philippe ULg et al

in Journal of Chromatography. A (1996), 750(1-2), 351-60

A fully automated method for the simultaneous determination of verapamil and its main metabolite norverapamil in human plasma is described. This method is based on on-line sample preparation using ... [more ▼]

A fully automated method for the simultaneous determination of verapamil and its main metabolite norverapamil in human plasma is described. This method is based on on-line sample preparation using dialysis followed by clean-up and enrichment of the dialysate on a precolumn and subsequent HPLC analysis with fluorometric detection. All sample handling operations were performed automatically by a sample processor equipped with a robotic arm (ASTED system). The plasma samples were dialysed on a cellulose acetate membrane (cut-off: 15 kD) and the dialysate was purified and enriched on a short pre-column filled with cyanopropyl silica. Before starting dialysis, this trace enrichment column (TEC) was first conditioned with the HPLC mobile phase and then with pH 3.0 acetate buffer. 370 microliters of plasma sample spiked with the internal standard (gallopamil) were dialysed in the static-pulsed mode. The solution at the donor side was pH 3.0 acetate buffer containing Triton X-100 while the acceptor solution was made of the same acetate buffer. When dialysis was discontinued, the analytes were desorbed from the TEC by the HPLC mobile phase and transferred to the C18 analytical column by means of a switching valve. This mobile phase consisted of a mixture of acetonitrile, pH 3.0 acetate buffer and 2-aminoheptane. The influence of different parameters of the dialysis process on the recovery of verapamil and norverapamil has been studied. The effect of the volume, the aspirating and dispensing flow-rates of the dialysis solution has been investigated. The recoveries of verapamil and norverapamil in plasma were close to 75% and the limits of quantification were 5 ng/ml for both analytes. The method was found to be linear in the concentration range from 5 to 500 ng/ml (r2: 0.9996 for both analytes). The intra-day and inter-day reproducibilities at a concentration of 100 ng/ml were 2.3% and 5.6% for verapamil and 1.7% and 5.1% for norverapamil, respectively. [less ▲]

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See detailResolution Improvement by Use of Carboxymethyl-Beta-Cyclodextrin as Chiral Additive for the Enantiomeric Separation of Basic Drugs by Capillary Electrophoresis
Fillet, Marianne ULg; Bechet, I.; Hubert, Philippe ULg et al

in Journal of Pharmaceutical & Biomedical Analysis (1996), 14(8-10), 1107-14

Three beta-cyclodextrin derivatives--carboxymethyl-, dimethyl- and hydroxypropyl-beta-cyclodextrin--were tested as chiral selectors for the enantioseparation of seven basic drugs in free solution ... [more ▼]

Three beta-cyclodextrin derivatives--carboxymethyl-, dimethyl- and hydroxypropyl-beta-cyclodextrin--were tested as chiral selectors for the enantioseparation of seven basic drugs in free solution capillary electrophoresis, using buffers made of 100 mM phosphoric acid adjusted to pH 3.0 with triethanolamine in fused silica capillaries thermostatted at 15 degrees C. The best results with respect to chiral resolution were obtained with carboxymethyl-beta-cyclodextrin (CMCD): the enantiomers of all compounds examined were completely resolved with this beta-cyclodextrin derivative. The influence of the CMCD concentration on the migration times, the apparent electrophoretic mobility difference and the resolution of the drug enantiomers was investigated thoroughly. Particularly impressive resolution values, up to 23.7, were obtained for several compounds in these capillary electrophoretic systems, using CMCD in the 5-15 mM concentration range. [less ▲]

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See detailSFSTP guide on validation of bioanalytical methods : a proposal
Bervoas-Martin, S.; Boulanger, Bruno ULg; Chapuzet, E. et al

Conference (1996)

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See detailDetermination of water-soluble vitamins by capillary zone electrophoresis
Fotsing, Lucas ULg; Bechet, I.; Fillet, Marianne ULg et al

in Journal de Pharmacie de Belgique (1996), 51

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See detailEnantiomeric separation of acidic and basic drugs by capillary electrophoresis using neutral and anionic cyclodextrins
Fillet, Marianne ULg; Bechet, I.; Schomburg, G. et al

in Journal de Pharmacie de Belgique (1996), 51

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See detailLC determination of oxprenolol enantiomers in human plasma using dialysis as on-line sample preparation technique
Ceccato, Attilio ULg; Toussaint, B.; Chiap, Patrice ULg et al

in Journal de Pharmacie de Belgique (1996), 51

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See detailMéthodes chromatographiques de dosage dans les milieux biologiques : Stratégie de validation
Bervoas-Martin, S.; Boulanger, Bruno ULg; Chapuzet, E. et al

Conference (1996)

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