References of "Hubert, Philippe"
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See detailStudy of adsorption effects in the analysis of pharmaceutical formulations by CE
Fotsing, L.; Fillet, Marianne ULiege; Chiap, Patrice ULiege et al

in Journal de Pharmacie de Belgique (1999), 54

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See detailOptimization of a high performance thin layer chromatography method to quantify solanines in patatoes
Bodart, P.; Hubert, Philippe ULiege; Angenot, Luc ULiege et al

in Journal de Pharmacie de Belgique (1999), 54

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See detailAutomated Sample Preparation Techniques for the Determination of Drug Enantiomers in Biological Fluids Using Liquid Chromatography with Chiral Stationary Phases
Ceccato, Attilio ULiege; Toussaint, B.; Chiap, Patrice ULiege et al

in Enantiomer (1999), 4(3-4), 305-15

The determination of drug enantiomers has become of prime importance in the field of pharmaceutical and biomedical analysis. Liquid chromatography (LC) is one of the most frequently used techniques for ... [more ▼]

The determination of drug enantiomers has become of prime importance in the field of pharmaceutical and biomedical analysis. Liquid chromatography (LC) is one of the most frequently used techniques for achieving the separation and quantitation of the enantiomers of drug compounds. In the bioanalytical field, the integrated systems present an interesting alternative to time-consuming sample preparation techniques such as liquid-liquid extraction. Solid phase extraction (SPE) on disposable cartridges, dialysis or column switching are sample preparation techniques that can be fully automated and applied to enantioselective analysis in biological fluids. The selection of the most appropriate LC mode and chiral stationary phase for enantioseparations in bioanalysis is discussed and some aspects of these automated sample preparation procedures are compared, such as selectivity, detectability, elution of the analytes from the extraction sorbent, sample volume and analyte stability. [less ▲]

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See detailIn-line concentration and enantioseparation of clenbuterol by transient ITP-CE-UV
Toussaint, B.; Bergmann, J.; Hubert, Philippe ULiege et al

Poster (1999)

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See detailEnantiomeric separation of clenbuterol by transient ITP-CE-MS
Toussaint, B.; Bergmann, J.; Hubert, Philippe ULiege et al

Poster (1999)

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See detailElimination of adsorption effects in the analysis of water-soluble vitamins in pharmaceutical formulations by capillary electrophoresis.
Fotsing, Lucas; Fillet, Marianne ULiege; Chiap, Patrice ULiege et al

in Journal of Chromatography. A (1999), 853(1-2), 391-401

A tendency to an increase in migration times was observed when different water-soluble vitamins were analysed repeatedly in pharmaceutical preparations by capillary electrophoresis. In order to better ... [more ▼]

A tendency to an increase in migration times was observed when different water-soluble vitamins were analysed repeatedly in pharmaceutical preparations by capillary electrophoresis. In order to better understand the origin of this effect, the influence of the vitamins and the excipients, such as cellulose derivatives, was investigated. These studies indicated that the increase in analyte migration times was most probably due to the adsorption of different kinds of constituents to the capillary wall. Different rinsing procedures were tested in order to eliminate these unfavourable effects. A rinse of the capillary with a 25 mM sodium dodecyl sulfate (SDS) solution in the running buffer between successive runs was found to be particularly effective when the analysis was performed by free solution capillary zone electrophoresis (CZE). When the vitamins were determined by micellar electrokinetic chromatography (MEKC) using SDS as surfactant, a short capillary rinse with the running buffer was sufficient to obtain reproducible migration times. The CZE and MEKC methods developed were validated and compared. Both methods could be applied to the determination of water-soluble vitamins in different multivitamin formulations. [less ▲]

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See detailThe SFSTP guide on the validation of chromatographic methods for drug bioanalysis: from the Washington Conference to the laboratory
Hubert, Philippe ULiege; Chiap, Patrice ULiege; Crommen, Jacques ULiege et al

in Analytica Chimica Acta (1999), 391(2), 135-148

On the basis of the guidelines given in the Washington Conference report and the ICH (International Conference of Harmonisation) recommendations some suggestions about experimental design and data ... [more ▼]

On the basis of the guidelines given in the Washington Conference report and the ICH (International Conference of Harmonisation) recommendations some suggestions about experimental design and data evaluation are proposed by an SFSTP Commission dedicated to the validation of chromatographic methods in bioanalysis. In a series of meetings, members of this Commission have tried to elaborate a rational, practical and statistically reliable strategy to assure the quality of the analytical results generated. This strategy has been formalised in a guide and the main suggestions made by the Commission are summarised in the present paper. The SFSTP guide has been produced to help analysts from the pharmaceutical industry to validate their bioanalytical methods, It is the result of a consensus between professionals having expertise in bioanalytical and/or statistical fields. The suggestions presented in this paper should therefore help the analyst to design and perform the minimum number of validation experiments needed to obtain all the required information to establish and demonstrate the reliability of its analytical procedure. The SFSTP guide suggests a validation strategy in two steps: a pre-validation and the validation itself. An experimental design is described for each of these steps and the main aspects discussed in the paper are related to the selection of the most appropriate calibration model to fit experimental data and the most suitable way to determine the limit(s) of quantitation and subsequently the calibration range as well as the optimum number of experiments to be performed in the validation phase. (C) 1999 Elsevier Science B.V. All rights reserved. [less ▲]

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