References of "Hubert, Philippe"
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See detailAutomated determination of drugs in ibological fluids using solid-phase extraction coupled to HPLC
Hubert, Philippe ULg

in Dissertation abstracts international (1995)

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See detailEnantiomeric separations by capillary electrophoresis applications in pharmaceutical, biomedical and environmental analysis, chapitre #L-7
Crommen, Jacques ULg; Fillet, Marianne ULg; Bechet, Isabelle et al

in Buszewski, B. (Ed.) New analytical methods for environmental control and monitoring (1995)

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See detailCZE separation of basic and acidic drug by use of cyclodextrin additives
Bechet, I.; Fillet, Marianne ULg; Fotsing, Lucas ULg et al

Poster (1995)

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See detailBiopharmaceutical aspects of the development of a sulfamethazine oral sustained release bolus for lambs
Evrard, Brigitte ULg; Delahaut, P.; Hubert, Philippe ULg et al

in Journal of Controlled Release (1995), 35

Pharmacokinetic parameters of sulfamethazine (SMZ) administered intravenously or orally either as an aqueous solution or as a lipid matrix formulation, were determined in young lambs. The value of the ... [more ▼]

Pharmacokinetic parameters of sulfamethazine (SMZ) administered intravenously or orally either as an aqueous solution or as a lipid matrix formulation, were determined in young lambs. The value of the rate constant for elimination (ke) for the intravenous solution was 0.18 h−1 compared to 0.10 h−1 for the oral aqueous solution. The absolute bioavailability of the oral solution was about 75%. A lipid matrix containing SMZ and a high density excipient is able to be retained in the reticulo-rumen and to produce a sustained release of the drug for at least 100 h provided that the mechanical strength of the bolus is sufficient. The pharmacokinetic data obtained with the lipid matrix show a release profile with two pulses due to both diffusion and erosion mechanisms. Plasma levels are maintained above the MIC of SMZ during 100 h with an absolute bioavailability of 51.7% [less ▲]

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See detailDetermination of Benzodiazepines by Micellar Electrokinetic Chromatography
Bechet, I.; Fillet, Marianne ULg; Hubert, Philippe ULg et al

in Electrophoresis (1994), 15(10), 1316-21

A method for the separation and determination of benzodiazepines by micellar electrokinetic chromatography (MEKC) has been developed. Separation buffers consisted of aqueous solutions of glycine and ... [more ▼]

A method for the separation and determination of benzodiazepines by micellar electrokinetic chromatography (MEKC) has been developed. Separation buffers consisted of aqueous solutions of glycine and triethanolamine (pH 9.0), containing sodium dodecyl sulfate (SDS) as surfactant and methanol as organic modifier. The effect of the concentration of SDS, methanol, glycine and triethanolamine on migration times and resolution was studied. Ten benzodiazepines were baseline separated at a 25 mM SDS concentration and 20% v/v methanol in a 75 mM glycine-250 mM triethanolamine buffer. Under these conditions, the within-day reproducibilities were 0.3-0.5% for migration times and 1.7-1.9% for peak areas at a concentration of 10 micrograms/mL. The limits of detection and quantification for oxazepam were 0.2 and 0.7 micrograms/mL, respectively, using an injection time of 5 s. [less ▲]

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See detailChiral Separation of Basic Drugs by Capillary Zone Electrophoresis with Cyclodextrin Additives
Bechet, I.; Paques, P.; Fillet, Marianne ULg et al

in Electrophoresis (1994), 15(6), 818-23

The enantiomers of a series of basic drugs were separated in capillary zone electrophoresis (CZE) in phosphate buffers, pH 3, containing beta-cyclodextrin or one of its derivatives as chiral selectors and ... [more ▼]

The enantiomers of a series of basic drugs were separated in capillary zone electrophoresis (CZE) in phosphate buffers, pH 3, containing beta-cyclodextrin or one of its derivatives as chiral selectors and uncoated fused silica capillaries thermostated at 15 degrees C. The nature of the cationic component of the background electrolyte was found to have a significant influence on achiral resolution and peak symmetry. The best results were obtained with triethanolamine, which was then used to adjust the buffer pH in all further experiments. The effects on chiral resolution of the nature and concentration of cyclodextrin, of the addition of methanol, and of capillary temperature were studied. Maximum resolution was obtained at a particular cyclodextrin concentration for each analyte, depending on the affinity of the analyte for this cyclodextrin. On the basis of the results, the effects of methanol addition and temperature on enantiomeric resolution could be explained and predicted. Numerous chiral separations are presented and suggestions for the rapid optimization of CZE enantioseparations with cyclodextrin additives are given. [less ▲]

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