Design of gradient coils for an X-band ESR 2D-imager.

Poster (2005)

ESR evidence of cholesterol extraction from liposome and cell membrane induced by RAMEB

Conference (2005)

Effects of COX-2 inhibitors on ROS produced by Chlamydia pneumoniae-primed human promonocytic cells (THP-1)

in Biochemical and Biophysical Research Communications (2004), 325(4), 1122-1130

Chronic inflammation through foam cells and macrophages is important in atherosclerosis development, and can be considered as therapeutic targets. Cyclooxygenase and NADPH-oxidase were expressed within ... [more ▼]

Chronic inflammation through foam cells and macrophages is important in atherosclerosis development, and can be considered as therapeutic targets. Cyclooxygenase and NADPH-oxidase were expressed within atherosclerotic lesions. Reactive oxygen species produced by NADPH oxidase were found to trigger the cyclooxygenase-2 expression. The effects of preferential COX-2 inhibitors on ROS produced by Chlamydia-primed human monocytes (THP-1 cells) were evaluated by fluorescence, chemiluminescence, oxymetry, and EPR spin trapping. Fluorescence assays showed an increased production of ROS with Chlamydia versus cells primed by 10(-8) M PMA. COX-2 inhibitors inhibited in a dose-dependent manner the luminol-enhanced CL while ibuprofen and diclofenac increased the chemiluminescence response. By EPR spin trapping, COX-2 inhibitors, ibuprofen, and diclofenac, exhibited a dose-dependent inhibiting effect (10 and 100 muM) on the EPR signal appearance. Our cell model combining EPR, chemiluminescence, and oxymetry appeared relevant to study the modulating effects of preferential COX-2 inhibitors on the cell oxidant activity and chronic inflammatory diseases. (C) 2004 Elsevier Inc. All rights reserved. [less ▲]

Improvement by solubilization in DMPC liposomes of PPME photodynamic effect - A study in human colon cancer cells HCT-116

Conference (2004)

Destruction of stearic acid nitroxyl radical mediated by photoecited pyropheophorbide-a methyl ester lipososmal systems

Conference (2004)

Effects of COX-2 inhibitors on ROS produced by Chlamydia pneumoniae-primed human promonocytic cells (THP-1)

in Biochemical and Biophysical Research Communications (2004), 325(4), 1122-1130

Chronic inflammation through foam cells and macrophages is important in atherosclerosis development, and can be considered as therapeutic targets. Cyclooxygenase and NADPH-oxidase were expressed within ... [more ▼]

Chronic inflammation through foam cells and macrophages is important in atherosclerosis development, and can be considered as therapeutic targets. Cyclooxygenase and NADPH-oxidase were expressed within atherosclerotic lesions. Reactive oxygen species produced by NADPH oxidase were found to trigger the cyclooxygenase-2 expression. The effects of preferential COX-2 inhibitors on ROS produced by Chlamydia-primed human monocytes (THP-1 cells) were evaluated by fluorescence, chemiluminescence, oxymetry, and EPR spin trapping. Fluorescence assays showed an increased production of ROS with Chlamydia versus cells primed by 10 8 M PMA. COX-2 inhibitors inhibited in a dose-dependent manner the luminol-enhanced CL while ibuprofen and diclofenac increased the chemiluminescence response. By EPR spin trapping, COX-2 inhibitors, ibuprofen, and diclofenac, exhibited a dose-dependent inhibiting effect (10 and 100 lM) on the EPR signal appearance. Our cell model combining EPR, chemiluminescence, and oxymetry appeared relevant to study the modulating effects of preferential COX-2 inhibitors on the cell oxidant activity and chronic inflammatory diseases. [less ▲]

ESR quantification of DMPC liposome viscosity change by the addition of cholesterol and/or cyclodextrin

Conference (2004)

The electronic states of 2-furanmethanol (furfuryl alcohol) studied by photon absorption and electron impact spectroscopies

in Journal of Chemical Physics (2003), 119(14), 7282-7288

The photoelectron spectrum of 2-furanmethanol (furfuryl alcohol) has been measured for ionization energies between 8 and 11.2 eV and the first three ionization bands assigned to pi(3), pi(2), and n(o ... [more ▼]

The photoelectron spectrum of 2-furanmethanol (furfuryl alcohol) has been measured for ionization energies between 8 and 11.2 eV and the first three ionization bands assigned to pi(3), pi(2), and n(o) ionizations in order of increasing binding energy. The photoabsorption spectrum has been recorded in the gas phase using both a synchrotron radiation source (5-9.91 eV, 248-125 nm) and electron energy-loss spectroscopy under electric-dipole conditions (5-10.9 eV, 248-90 nm). The (UV) absorption spectrum has also been recorded in solution (4.2-6.36 eV, 292-195 nm). The electronic excitation spectrum appears to be dominated by transitions between pi and pi* orbitals in the aromatic ring, leading to the conclusion that the frontier molecular orbitals of furan are affected only slightly on replacement of a H atom by the -CH2OH group. Additional experiments investigating electron impact at near-threshold energies have revealed two low-lying triplet states and at least one electron/molecule shape resonance. Dissociative electron attachment also shows to be widespread in furfuryl alcohol. (C) 2003 American Institute of Physics. [less ▲]

Investigation of singlet oxygen reactivity towards propofol

in Photochemical & Photobiological Sciences (2003), 2(9), 939-945

The reaction between the anaesthetic agent 2,6-diisopropylphenol (propofol, PPF) and singlet oxygen (1O2) has been investigated in aqueous solution by means of HPLC, GC, absorption spectroscopy and laser ... [more ▼]

The reaction between the anaesthetic agent 2,6-diisopropylphenol (propofol, PPF) and singlet oxygen (1O2) has been investigated in aqueous solution by means of HPLC, GC, absorption spectroscopy and laser flash photolysis withinfrared luminescence detection. The reaction of propofol with singlet oxygen produced by light irradiation of Rose Bengal leads essentially to two reaction products, 2,6-diisopropyl-p-benzoquinone and 3,5,3',5'-tetraisopropyl-(4,4')-diphenoquinone that are unambiguously identified from comparison with authentic samples [less ▲]

A spectroscopic determination of singlet oxygen quantum yield of PPME in different solvents

Conference (2003)