References of "Heinen, Ernst"
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See detailConsiderations sur les structures dites lamellaires de l'oeuf de rat non feconde.
Baeckeland, E.; Heinen, Ernst ULg

in Bulletin de l'Association des Anatomistes (1980), 64(185), 173-81

The ultrastructural study of the unfertilized Rat egg ooplasm has shown that the so-called lamellar structures are in fact filaments constituted of proteins and where no ribonucleic acid can be detected ... [more ▼]

The ultrastructural study of the unfertilized Rat egg ooplasm has shown that the so-called lamellar structures are in fact filaments constituted of proteins and where no ribonucleic acid can be detected. Thus, they do not represent a particular organization of polyribosomes. These filaments could be considered as protein stockage, maybe from membranous origin, ready for use during segmentation. Several figures suggest that endocytotic vesicles and vacuoli constitute the source of the filamentous material. [less ▲]

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See detailModifications des membranes foetales de la ratte apres amniocentese (observations preliminaires).
Baeckeland, E.; Heinen, Ernst ULg

in Bulletin de l'Association des Anatomistes (1979), 63(180), 59-67

With the aid of scanning electron microscopy the normal pattern of both faces of amnion and visceral yolk sac were described. The inner face of the amnion turned towards the amniotic cavity is overlaid by ... [more ▼]

With the aid of scanning electron microscopy the normal pattern of both faces of amnion and visceral yolk sac were described. The inner face of the amnion turned towards the amniotic cavity is overlaid by large pavimentous cells bearing numerous microvilli. The opposite face, which delimits the exocoelome, consists of flattened fibroblasts separated by a fundamental connective substance. The two faces of the visceral yolk sac also show very different patterns. The exocoelomic face is covered by pavimentous cells ; these are smaller and bear less microvilli than amniotic cells. The yolk sac endoderm appears built up with prismatic or cubic cells, whose apical part is bulging and possesses a dense covering of microvilli. Amniocentesis effects are then reported. This intervention seems to provoke fetal membrane modifications only after 15 hours. These modifications essentially concern the visceral yolk sac whose exocoelomic cells are covered by more numerous microvilli which may show an intensification of a secretory process. [less ▲]

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See detailEffects of antimitotic agents either free or bound to DNA on mouse peritoneal macrophages cultivated in vitro.
Heinen, Ernst ULg

in Virchows Archiv. B : Cell pathology (1978), 27(1), 79-87

Mouse peritoneal macrophages were cultivated in vitro and treated with ethidium bromide (EB) or with cis-dichloro-diammine platinum (II) (cis-Pt). EB provokes strong cytological alterations and cell ... [more ▼]

Mouse peritoneal macrophages were cultivated in vitro and treated with ethidium bromide (EB) or with cis-dichloro-diammine platinum (II) (cis-Pt). EB provokes strong cytological alterations and cell degeneration; cis-Pt was not toxic under our experimental contitions. EB-DNA complex penetrates into the macrophages, is liberated from DNA in vacuoles, then diffuses into the cell and is highly cytotoxic. Cis-Pt-DNA complex also penetrates into the cells, but cis-Pt cannot be released from DNA, cis-Pt-DNA complex accumulates inside cytoplasmic vacuoles but has no cytotoxic activity. [less ▲]

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See detailEtude des polysaccharides presents dans les cretes palatines du rat peu avant leur fusion.
Baeckeland, E.; Heinen, Ernst ULg

in Bulletin de l'Association des Anatomistes (1978), 62(176), 65-76

When slices of rat palatal shelves removed by dissection from 16 days 8 hours old fetuses, just before fusion, are treated by the periodic acid Schiff reaction (PAS), colored substances may be detected ... [more ▼]

When slices of rat palatal shelves removed by dissection from 16 days 8 hours old fetuses, just before fusion, are treated by the periodic acid Schiff reaction (PAS), colored substances may be detected especially in the epithelium of the potential fusion zone; these substances are normally observed at the free surface of the epithelium. Our histochemical research, realized with the aid of the photonic microscope and of the transmission electron microscope, shows that most of the PAS positive material consists in glycogen; at the surface, this polysaccharide remains in the cytoplasm of flat degenerating cells and cannot be confused with the "cell coat" which presents no particularity, at this level, after demonstration by ruthenium red and by lanthanum nitrate. [less ▲]

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See detailAptitude a fusionner de cretes palatines de rat cultivees in vitro.
Pierlet, M.; Heinen, Ernst ULg

in Archives de Biologie (1978), 89(3), 363-90

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See detailModifications histo-physiologiques de l'amnios chez la ratte en fin de gestation.
Baeckeland, E.; Heinen, Ernst ULg; Papart, Luc ULg

in Comptes Rendus des Séances de la Société de Biologie et de ses Filiales (1978), 172(2), 374-6

Amniotic epithelium of the rat has been studied during the eight last days of pregnancy, by scanning electron microscope analysis. The cells of this organ progressively become larger and flatter. Changes ... [more ▼]

Amniotic epithelium of the rat has been studied during the eight last days of pregnancy, by scanning electron microscope analysis. The cells of this organ progressively become larger and flatter. Changes on their surface have also been observed : density of microvilli increases but single cilia disappear. These changes are related to modifications in the exchange processes. [less ▲]

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See detailResorption peritoneale de bromure d'ethidium, libre du lie a de l'ADN, chez des rongeurs.
Heinen, Ernst ULg; Calberg-Bacq, C. M.; Bassleer, R.

in Comptes Rendus des Séances de la Société de Biologie et de ses Filiales (1978), 172(4), 801-4

Ethidium bromide, either free (EB) or bound to DNA (EB-DNA), is injected into the peritoneal cavity of adult rats or mice. EB is then detected by fluorescence microscopy in peritoneal cells and by ... [more ▼]

Ethidium bromide, either free (EB) or bound to DNA (EB-DNA), is injected into the peritoneal cavity of adult rats or mice. EB is then detected by fluorescence microscopy in peritoneal cells and by spectrophotometry in the peritoneal fluid. EB-DNA persists for a longer period of time in the peritoneal cavity than free EB does. [less ▲]

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See detailCis-dichloro-diammine platinum (II) as stain for electron microscopic preparations.
Heinen, Ernst ULg

in Histochemistry (1977), 51(2-3), 257-60

Cis-dichloro-diammine platinum (II) provides a strong contrast to electron microscopic preparations by reacting with nucleic acids and proteins. This staining technique is easy and applicable to ultrathin ... [more ▼]

Cis-dichloro-diammine platinum (II) provides a strong contrast to electron microscopic preparations by reacting with nucleic acids and proteins. This staining technique is easy and applicable to ultrathin sections embedded in Epon; various staining conditions have been tested. [less ▲]

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See detailEtude tridimensionnelle du noyau de fibroblastes en culture in vitro.
Bassleer, R.; Heinen, Ernst ULg

in Archives de Biologie (1977), 88(1), 53-9

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See detailObservations au microscope electronique a balayage de cellules nerveuses de moelle spinale d'embryons de poulet cultivees in vitro sur de la polylysine-L.
Noel-Courtey, B.; Heinen, Ernst ULg

in Comptes Rendus des Séances de l'Académie des Sciences. Série D : Sciences Naturelles (1977), 285(4), 385-7

Chick embryo nerve cells from the lumbo-sacral spinal cord have been isolated by trypsinisation and cultivated in Rose chambers on polylysine-L as a substrate. The cells are analysed by scanning electron ... [more ▼]

Chick embryo nerve cells from the lumbo-sacral spinal cord have been isolated by trypsinisation and cultivated in Rose chambers on polylysine-L as a substrate. The cells are analysed by scanning electron microscopy. The mode of adherence of these cells to the substrate and the modifications of the surface of the neuroblasts during their transformation into neurocytes are studied. [less ▲]

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See detailComparaison des effets du bromure d'ethidium et du complexe bromure d'ethidium-acide desoxyribonucleique dans des cellules tumorales d'Ehrlich.
Desaive, Claude ULg; Heinen, Ernst ULg; Bassleer, R. et al

in Comptes Rendus des Séances de l'Académie des Sciences. Série D : Sciences Naturelles (1977), 284(15), 1485-8

When injected into the peritoneal cavity, ethidium bromide can strongly inhibit the multiplication of mouse Ehrlich ascites tumour cells. This antitumour effect is increased when ethidium bromide is ... [more ▼]

When injected into the peritoneal cavity, ethidium bromide can strongly inhibit the multiplication of mouse Ehrlich ascites tumour cells. This antitumour effect is increased when ethidium bromide is linked to DNA and also injected into the peritoneal cavity. The cellular alterations are identical after a treatment with E.B. either free or bound to DNA. However, when the cells are treated with E.B.-DNA they contain E.B. for a longer period than after a treatment with E.B. [less ▲]

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See detailEtude au microscope electronique a balayage et a transmission de l'epithelium amniotique chez la ratte en fin de gestation.
Baeckeland, E.; Heinen, Ernst ULg; Papart, Luc ULg

in Bulletin de l'Association des Anatomistes (1977), 61(172), 81-91

The pattern of the amniotic epithelium during the eight last days of pregnancy is described with the aid of scanning and transmission microscopy. A flattening of the cells appears near the term. Their ... [more ▼]

The pattern of the amniotic epithelium during the eight last days of pregnancy is described with the aid of scanning and transmission microscopy. A flattening of the cells appears near the term. Their area becomes progressively greater increasing their contact surface with the amniotic fluid. Simultaneaously, their microvilli become more numerous. During the 20th, the 21st and the 22nd days of pregnancy, we observed cytoplasmic processes emanating from the base of the cell interrupting the basal membrane and entering the underlying connective substance. No evidence of cell degeneration was seen. [less ▲]

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See detailContribution a l'etude des effets d'agents antimitotiques sur des cellules animales normales ou cancereuses. Analyses cytologiques et cytochimiques quantitatives.
Bassleer, R.; Goessens, Guy ULg; Lepoint, A. et al

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1977), 13(10-12), 496-502

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See detailEtude au microscope electronique a balayage du palais secondaire en formation chez le rat.
Baeckeland, E.; Heinen, Ernst ULg

in Bulletin de l'Association des Anatomistes (1977), 61(172), 61-70

Normal fetuses 14 to 17 days old were obtained from female Wistar rats. The palates have been studied, after dissection, by scanning electron microscopy. Palatal closure is achieved, in the median and ... [more ▼]

Normal fetuses 14 to 17 days old were obtained from female Wistar rats. The palates have been studied, after dissection, by scanning electron microscopy. Palatal closure is achieved, in the median and posterior region, by the elevation of the lateral palatine processus from a ventro-medial to a horizontal position; immediately after the horizontalization, they adhere to each other. Rostrally naso-palatine canal closure takes place later; it results from an epithelial and mesenchymal outgrowth under the nasal septum. The mutual adhesivity of the free edges of the lateral processes is noticeable. The pattern of the fusion surface has been considered, elements such as exfoliated cells, filopodes and microvilli are present. Their contribution to the adherence phenomenon is discussed. [less ▲]

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See detailEffets du complexe cis-dichlorodiammino-platine(II) - acide désoxyribonucléique sur des cellules normales ou cancéreuses
Heinen, Ernst ULg; Desaive, Claude ULg; Houssier, Claude ULg et al

in Comptes Rendus des Séances de la Société de Biologie et de ses Filiales (1976), 170(4), 919-921

The effects of cis-dichloro-diammino-platinum(II) (cis-Pt) bound to DNA have been compared to those of free cis-Pt in mouse Ehrlich tumor cells, in peritoneal macrophages and in chick embryo fibroblasts ... [more ▼]

The effects of cis-dichloro-diammino-platinum(II) (cis-Pt) bound to DNA have been compared to those of free cis-Pt in mouse Ehrlich tumor cells, in peritoneal macrophages and in chick embryo fibroblasts cultivated in vitro.Cis-Pt has no antimitotic activity anymore when linked tp DNA. This would be due to the fact that free cis-Pt is not released from cis-Pt-DNA complex inside lysosomes. [less ▲]

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See detailComparative ultrastructural study of the effects of serum-free medium and dibutyryl-cyclic AMP on newborn rat astroblasts.
Moonen, Gustave ULg; Heinen, Ernst ULg; Goessens, Guy ULg

in Cell & Tissue Research (1976), 167(2), 221-7

Modifications of cell shape induced in cultured newborn rat astroblasts by serum deprivation or dibutyryladenosine 3'-5' monophosphate (dBcAMP) are described. Serum suppression modifies the adhesitivity ... [more ▼]

Modifications of cell shape induced in cultured newborn rat astroblasts by serum deprivation or dibutyryladenosine 3'-5' monophosphate (dBcAMP) are described. Serum suppression modifies the adhesitivity of the cells to the substrate, but this modification is not consistent with a true differentiation. The main ultrastructural feature of dBcAMP-treated astroblasts is the presence of an extensive system of 90 A microfilaments, while control cells are relatively devoid of these structures. [less ▲]

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See detailMode of action of cis-dichloro-diammine platinum(II) on mouse Ehrlich ascites tumour cells.
Heinen, Ernst ULg; Bassleer, R.

in Biochemical Pharmacology (1976), 25(16), 1871-5

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See detailCytological and cytochemical analysis of the effects of cis-dichlorodiamino platinum (II) on chick fibroblasts cultivated in vitro.
Heinen, Ernst ULg; Bassleer, R.

in Chemotherapy (1976), 22(3-4), 253-61

Chick embryo fibroblasts cultivated in vitro and treated with cis-dichlorodiamino platinum (II) have been analysed by cytological and cytochemical methods. Morphological alterations (in the nucleoli, in ... [more ▼]

Chick embryo fibroblasts cultivated in vitro and treated with cis-dichlorodiamino platinum (II) have been analysed by cytological and cytochemical methods. Morphological alterations (in the nucleoli, in the chromatin and in the cytoplasm), inhibition of DNA and RNA syntheses and of cell multiplication have been noted. Under some conditions, cells are blocked just before entering into mitosis (G2 block) and can become polyploid. A high degree of cell degeneration has also been observed. [less ▲]

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