References of "Heinen, Ernst"
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See detailThe role of the cell coat in palatal shelves adhesion
Heinen, Ernst ULg; Baeckeland, E.; Renard, A. M.

in Lectins Biology, Biochemistry, Clinical Biochemistry (1982)

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See detailRelations entre immunogénicité et différenciation des métastases d'un mélanome expérimental chez la souris.
Heinen, Ernst ULg; Beckers, Josiane ULg; Desaive, Claude ULg et al

in Comptes Rendus des Séances de la Société de Biologie et de ses Filiales (1982), 176(2), 223-6

The graft into the footpad of Jackson mice of B16 melanoma cells which are only slightly pigmented, induces the simultaneous appearance of achromatic and well-pigmented metastatics. We have analysed ... [more ▼]

The graft into the footpad of Jackson mice of B16 melanoma cells which are only slightly pigmented, induces the simultaneous appearance of achromatic and well-pigmented metastatics. We have analysed histologically both types of metastases from the 8th to the 20th day after the resection of the primary tumor. The percentages of mitoses are higher in the achromatic metastases than in the pigmented ones. Both are infiltrated by lymphocytes and by a small number of macrophages and polymorphonuclears. A statistical analysis demonstrates however that the percentage of lymphocytes is not related to the importance of the metastase pigmentation. The discussion is devoted to the relations between the infiltration by lymphocytes, the immunogenicity and the differentiation of the tumor. [less ▲]

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See detailFixation de ferritine cationisee a la surface des cretes palatines du rat.
Renard, Ariane ULg; Heinen, Ernst ULg; Baeckeland, E.

in Bulletin de l'Association des Anatomistes (1982), 66(192), 117-25

Recently, we suggested that the cell coat of the surface epithelial cells of the palatal shelves and especially its sialyl groups are implied in the adhesion process which initiates the constitution of ... [more ▼]

Recently, we suggested that the cell coat of the surface epithelial cells of the palatal shelves and especially its sialyl groups are implied in the adhesion process which initiates the constitution of the secondary palate. In this work, use of cationized ferritin which interacts with anionic receptors of the plasma membrane give us the possibility to render these sialic acids visible. After five minutes incubation in presence of cationized ferritin, the labeling particles are distributed in monolayer over the plasmalemma of epithelial cells. Subsequent incubation in cationized ferritin free medium results in clustering of the marker followed by the detachment of the labeled patches. Otherwise, incubation in the presence of neuraminidase from Clostridium perfringens before the polycationic ligand contact prevents labeling. We think that lateral migration modifications of the anionic receptor could be associated with changes of the shelves capacity to adhere. [less ▲]

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See detailHistological structure and functions of synovium.
Bassleer, R.; Lhoest-Gauthier, M. P.; Renard, A. M. et al

in Proceedings of the international symposium on articular synovium (1982)

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See detailMobility of Con A receptors at the surface of palatal shelves before the closure of the secundary palate
Baeckeland, E.; Heinen, Ernst ULg; Renard, A. M.

in Lectins Biology, Biochemistry, Clinical Biochemistry (1982)

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See detailEffets du cis-dichlorodiammineplatine (II) (cis-Pt II) sur l'ultrastructure de la chromatine de cellules animales ou vegetales et repercussions sur le cycle cellulaire.
De Pauw, Marie-Claire ULg; Heinen, Ernst ULg; Houssier, Claude ULg et al

in Comptes Rendus des Séances de la Société de Biologie et de ses Filiales (1982), 176(3), 364-8

Cultivated animal cells (mouse peritoneal macrophages, chick fibroblasts or mouse Ehrlich tumor cells) or Zea mays root tips are treated with cis-Pt (II). Various effects (chromatin dispersion or ... [more ▼]

Cultivated animal cells (mouse peritoneal macrophages, chick fibroblasts or mouse Ehrlich tumor cells) or Zea mays root tips are treated with cis-Pt (II). Various effects (chromatin dispersion or condensation, pycnosis) are observed under some experimental conditions in all cell types. Cytoplasmic ribosomes in helical aggregates appear but only in vegetal cells. Mitosis and cell cycle disturbances due to cis-Pt (II) are probably related to chromatin alterations. We suggest that the latter and helical polyribosomes are produced by cis-Pt (II) reaction with nucleic acids in these structures. [less ▲]

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See detailEtude ultrastructurale des effets de la neuraminidase sur des processus palatins de rat
Renard, Ariane ULg; Heinen, Ernst ULg; Baeckeland, E.

in Bulletin de l'Association des Anatomistes (1981), 65(190), 329-39

In a previous work, we demonstrated that palatine processes treated with 10 micrograms/ml neuraminidase for 30 minutes at 37 degrees C show a delayed fusion in vitro. In order to investigate the effects ... [more ▼]

In a previous work, we demonstrated that palatine processes treated with 10 micrograms/ml neuraminidase for 30 minutes at 37 degrees C show a delayed fusion in vitro. In order to investigate the effects of neuraminidase, the treated palatine processes were observed with the aid of transmission and scanning electron microscopy. The effect of neuraminidase is exclusively detectable on superficial flat cells of the palatine processes, especially the cells of the medial edge of the process. The epithelial basal cells remain unchanged. Observed with the S.E.M., the superficial epithelial cells show bubbles, become spherical and detached. To explain these observations, we namely propose the following hypothesis: the action of neuraminidase prevents the fusion of the palatine processes because it denudes basal cells bearing a too immature cell coat for ensuring sufficient adherence between the palatine processes. [less ▲]

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See detailNifurzide, a nitrofuran antiinfectious agent: interaction with Escherichia coli cells.
Delsarte, Anne; Faway, Michel; Frère, Jean-Marie et al

in Antimicrobial Agents and Chemotherapy (1981), 19(3), 477-86

This paper presents a study of the interactions between Escherichia coli cells and nifurzide, a nitrofuran derivative which is used as an intestinal antiinfectious agent. At low concentrations of ... [more ▼]

This paper presents a study of the interactions between Escherichia coli cells and nifurzide, a nitrofuran derivative which is used as an intestinal antiinfectious agent. At low concentrations of nifurzide, the growth rate of the cultures decreased, and elongated, nonseptate cells appeared. At high concentrations, complete growth inhibition occurred, accompanied by a rather strong bactericidal effect, but the appearance of the cells was normal; in particular, no bacteriolytic effect was observed. A very large number of antibiotic molecules were bound per bacterial cell. After cell disruption, similar amounts of nifurzide were found in the cytoplasm, cytoplasmic membranes, and cell wall, respectively. Most of the bound nifurzide was rapidly degraded or became protein bound. The structure of the outer membrane lipopolysaccharide appeared to have little influence on the activity of nifurzide. [less ▲]

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See detailScanning electron microscopic study on the interaction of liposomes with cells in vitro
Heinen, Ernst ULg; Gillet, Marie-Claire ULg

in European Journal of Cell Biology (1980), 22

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See detailSpecific effects of some phospholipids on the maturation of nervous cells cultivated in vitro
Vanalderweireldt, H.; De parmentier, Freddy; Balisaux, André et al

in European Journal of Cell Biology (1980), 22

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See detailEtude de la relation entre la structure chimique et l'activite antimitotique d'une serie nouvelle d'arylthioethers pyrimidiniques.
Jamoulle, J. C.; Lapiere, C. L.; Bassleer, R. et al

in Annales Pharmaceutiques Françaises (1980), 38(3), 267-70

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See detailConsiderations sur les structures dites lamellaires de l'oeuf de rat non feconde.
Baeckeland, E.; Heinen, Ernst ULg

in Bulletin de l'Association des Anatomistes (1980), 64(185), 173-81

The ultrastructural study of the unfertilized Rat egg ooplasm has shown that the so-called lamellar structures are in fact filaments constituted of proteins and where no ribonucleic acid can be detected ... [more ▼]

The ultrastructural study of the unfertilized Rat egg ooplasm has shown that the so-called lamellar structures are in fact filaments constituted of proteins and where no ribonucleic acid can be detected. Thus, they do not represent a particular organization of polyribosomes. These filaments could be considered as protein stockage, maybe from membranous origin, ready for use during segmentation. Several figures suggest that endocytotic vesicles and vacuoli constitute the source of the filamentous material. [less ▲]

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See detailModifications des membranes foetales de la ratte apres amniocentese (observations preliminaires).
Baeckeland, E.; Heinen, Ernst ULg

in Bulletin de l'Association des Anatomistes (1979), 63(180), 59-67

With the aid of scanning electron microscopy the normal pattern of both faces of amnion and visceral yolk sac were described. The inner face of the amnion turned towards the amniotic cavity is overlaid by ... [more ▼]

With the aid of scanning electron microscopy the normal pattern of both faces of amnion and visceral yolk sac were described. The inner face of the amnion turned towards the amniotic cavity is overlaid by large pavimentous cells bearing numerous microvilli. The opposite face, which delimits the exocoelome, consists of flattened fibroblasts separated by a fundamental connective substance. The two faces of the visceral yolk sac also show very different patterns. The exocoelomic face is covered by pavimentous cells ; these are smaller and bear less microvilli than amniotic cells. The yolk sac endoderm appears built up with prismatic or cubic cells, whose apical part is bulging and possesses a dense covering of microvilli. Amniocentesis effects are then reported. This intervention seems to provoke fetal membrane modifications only after 15 hours. These modifications essentially concern the visceral yolk sac whose exocoelomic cells are covered by more numerous microvilli which may show an intensification of a secretory process. [less ▲]

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See detailEffects of antimitotic agents either free or bound to DNA on mouse peritoneal macrophages cultivated in vitro.
Heinen, Ernst ULg

in Virchows Archiv. B : Cell pathology (1978), 27(1), 79-87

Mouse peritoneal macrophages were cultivated in vitro and treated with ethidium bromide (EB) or with cis-dichloro-diammine platinum (II) (cis-Pt). EB provokes strong cytological alterations and cell ... [more ▼]

Mouse peritoneal macrophages were cultivated in vitro and treated with ethidium bromide (EB) or with cis-dichloro-diammine platinum (II) (cis-Pt). EB provokes strong cytological alterations and cell degeneration; cis-Pt was not toxic under our experimental contitions. EB-DNA complex penetrates into the macrophages, is liberated from DNA in vacuoles, then diffuses into the cell and is highly cytotoxic. Cis-Pt-DNA complex also penetrates into the cells, but cis-Pt cannot be released from DNA, cis-Pt-DNA complex accumulates inside cytoplasmic vacuoles but has no cytotoxic activity. [less ▲]

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See detailEtude des polysaccharides presents dans les cretes palatines du rat peu avant leur fusion.
Baeckeland, E.; Heinen, Ernst ULg

in Bulletin de l'Association des Anatomistes (1978), 62(176), 65-76

When slices of rat palatal shelves removed by dissection from 16 days 8 hours old fetuses, just before fusion, are treated by the periodic acid Schiff reaction (PAS), colored substances may be detected ... [more ▼]

When slices of rat palatal shelves removed by dissection from 16 days 8 hours old fetuses, just before fusion, are treated by the periodic acid Schiff reaction (PAS), colored substances may be detected especially in the epithelium of the potential fusion zone; these substances are normally observed at the free surface of the epithelium. Our histochemical research, realized with the aid of the photonic microscope and of the transmission electron microscope, shows that most of the PAS positive material consists in glycogen; at the surface, this polysaccharide remains in the cytoplasm of flat degenerating cells and cannot be confused with the "cell coat" which presents no particularity, at this level, after demonstration by ruthenium red and by lanthanum nitrate. [less ▲]

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See detailAptitude a fusionner de cretes palatines de rat cultivees in vitro.
Pierlet, M.; Heinen, Ernst ULg

in Archives de Biologie (1978), 89(3), 363-90

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See detailModifications histo-physiologiques de l'amnios chez la ratte en fin de gestation.
Baeckeland, E.; Heinen, Ernst ULg; Papart, Luc ULg

in Comptes Rendus des Séances de la Société de Biologie et de ses Filiales (1978), 172(2), 374-6

Amniotic epithelium of the rat has been studied during the eight last days of pregnancy, by scanning electron microscope analysis. The cells of this organ progressively become larger and flatter. Changes ... [more ▼]

Amniotic epithelium of the rat has been studied during the eight last days of pregnancy, by scanning electron microscope analysis. The cells of this organ progressively become larger and flatter. Changes on their surface have also been observed : density of microvilli increases but single cilia disappear. These changes are related to modifications in the exchange processes. [less ▲]

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See detailResorption peritoneale de bromure d'ethidium, libre du lie a de l'ADN, chez des rongeurs.
Heinen, Ernst ULg; Calberg-Bacq, C. M.; Bassleer, R.

in Comptes Rendus des Séances de la Société de Biologie et de ses Filiales (1978), 172(4), 801-4

Ethidium bromide, either free (EB) or bound to DNA (EB-DNA), is injected into the peritoneal cavity of adult rats or mice. EB is then detected by fluorescence microscopy in peritoneal cells and by ... [more ▼]

Ethidium bromide, either free (EB) or bound to DNA (EB-DNA), is injected into the peritoneal cavity of adult rats or mice. EB is then detected by fluorescence microscopy in peritoneal cells and by spectrophotometry in the peritoneal fluid. EB-DNA persists for a longer period of time in the peritoneal cavity than free EB does. [less ▲]

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