References of "Heinen, Ernst"
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See detailImmunologie clinique
Heinen, Ernst ULg

Book published by De Boeck (1993)

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See detailRapid and Selective Isolation of Follicular Dendritic Cells by Low Speed Centrifugations on Discontinuous Bsa Gradients
Marcoty, C.; Heinen, Ernst ULg; Antoine, Nadine ULg et al

in Advances in Experimental Medicine and Biology (1993), 329

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See detailEmperipolesis of lymphoid cells by human follicular dendritic cells in vitro.
Tsunoda, R.; Nakayama, M.; Heinen, Ernst ULg et al

in Virchows Archiv. B : Cell pathology (1992), 62(2), 69-78

Isolated follicular dendritic cells (FDCs) showed true and pseudoemperipolesis of fresh tonsillar lymphocytes, even after long-term (50-day) cultivation. Emperipolesis by FDCs was not restricted by ... [more ▼]

Isolated follicular dendritic cells (FDCs) showed true and pseudoemperipolesis of fresh tonsillar lymphocytes, even after long-term (50-day) cultivation. Emperipolesis by FDCs was not restricted by allotype specificity, nor was it inhibited by the addition of antibodies against MHC-I & II antigens. Follicular dendritic cells predominantly engulfed B-cells; monocytes and macrophages were not found between FDC cytoplasmic extensions. When highly purified T-cell populations were added to FDC cultures emperipolesis of T-cells occurred, particularly those of the CD4-positive phenotype. Mitoses appeared within 6 h in the emperipolesed lymphocytes and, after an additional 18 h, some lymphocytes exhibited apoptosis. [less ▲]

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See detailLes communications intercellulaires au niveau des centres germinatifs.
Heinen, Ernst ULg

in Revue Médicale de Liège (1992), 47(3), 118-22

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See detailAtlas d'Histologie en couleurs
Amas-Costesec, A.; Heinen, Ernst ULg

Book published by De Boeck (1992)

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See detailThe germinal centre: a monastery or a bar?
Heinen, Ernst ULg; Tsunoda, T.; Marcoty, C. et al

in Research in Immunology (1991), 142(3), 242-4

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See detailGerminal centres and the immune response - Discussion
Cebra, J.; Schrader, C.; Shroff, K. et al

in Research in Immunology (1991), 142

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See detailAre germinal centers insulating microenvironments?
Heinen, Ernst ULg; Tsunoda, R.; Bosseloir, A. et al

in Lymphatic tissues and in vivo immune responses (1991)

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See detailUltrastructural and functional aspects of FDC in vitro
Heinen, Ernst ULg; Cormann, N.; Tsunoda, R. et al

in Accessory cells in HIV and other retroviral infections (1991)

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See detailIntercellular communications in lymph follicles
Heinen, Ernst ULg; Tsunoda, R.; Marcoty, C. et al

in Dendritic cells in lymphoid tissues (1991)

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See detailPurification and cultivation of follicular dendritic cells.
Marcoty, C.; Heinen, Ernst ULg; Bosseloir, A. et al

in Lymphatic tissues and in vivo immune responses (1991)

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See detailIL6 and IL4: localization and production in human tonsils
Bosseloir, A.; Hooghe-Peters, E.; Heinen, Ernst ULg et al

in Lymphatic tissues and in vivo immune responses (1991)

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See detailIsolation and long-term cultivation of human tonsil follicular dendritic cells.
Tsunoda, R.; Nakayama, M.; Onozaki, K. et al

in Virchows Archiv. B : Cell pathology (1990), 59(2), 95-105

Highly purified follicular dendritic cells (FDC) were isolated from human tonsils and cultivated for up to 150 days. The cell separation method employed produced pure aggregates (FDC-clusters) composed of ... [more ▼]

Highly purified follicular dendritic cells (FDC) were isolated from human tonsils and cultivated for up to 150 days. The cell separation method employed produced pure aggregates (FDC-clusters) composed of FDC and germinal center lymphoid cells, useful for the analysis of the relationship between these two cell types and of the behavior of FDC in culture. During the first few days of culture, lymphoid cells located between FDC extensions survived better than those which were free or partly covered by FDC. After 6 days, the lymphoid population degenerated and only the FDC survived. The unique antigenic pattern of FDC (positive for HLA-DR. DRC-1, CD14b, CD21, CD23, CD35) disappeared within a few days of culture. Recombinant interferon-gamma exerted a positive effect either on retaining HLA-DR expression or on the reexpression of these antigens by FDC. HLA-ABC antigens were traced until the 10th day and desmosomal junctions until the 14th day. Subsequently, FDC presented peculiar features, including oval and rhomboid shapes, one to ten nuclei, fine amoeboid extensions, stress fibers and a radical dense zone in their cytoplasm. FDC possessed actin, tubulin and vimentin, but neither desmin nor cytokeratin. After 40 days of culture, FDC enlarged and were covered with abundant membrane extensions. Even when kept as long as 150 days in vitro. FDC did not proliferate in any of the culture conditions employed. [less ▲]

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See detailMicroenvironments during antigen stimulation
Heinen, Ernst ULg; Bosseloir, A.; Cormann, N. et al

in Molecular biology of B cells developements (1990)

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See detailLes organes du système immunitaire
Heinen, Ernst ULg; Defresne, M. P.; Boniver, J. et al

in Immunologie Animale (1990)

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See detailIntercellular Contacts between Germinal Center Cells. Mechanisms of Adhesion between Lymphoid Cells and Follicular Dendritic Cells
Louis, Edouard ULg; Philippet, B.; Cardos, B. et al

in Acta Oto-Rhino-Laryngologica Belgica (1989), 43(4), 297-320

Intercellular connections exist between germinal center cells especially between lymphoid cells and follicular dendritic cells (FDC). Even after isolation, FDC remain associated to lymphocytes and are ... [more ▼]

Intercellular connections exist between germinal center cells especially between lymphoid cells and follicular dendritic cells (FDC). Even after isolation, FDC remain associated to lymphocytes and are able, in a cell suspension, to establish new connections with others. Using human tonsillar cells or mouse lymph node cells we analysed these connections which were shown to be species-specific. Low temperature as well as absence of Ca++ and Mg++ in the culture medium reduced the adherence of fluorochrome-labeled lymphoid cells to FDC. Colchicine treatment did not impair the adherence, whereas cytochalasin B dit it; this was the first observation underlining the importance of microfibrils in FDC. Antibodies directed towards integrin molecules (LFA-1 alpha or beta chain, CD11a and CD18 respectively) reduced the adherence, others (anti-CR3 or anti-gp 150/95, CD11b and c respectively) did not influence it. Antibodies directed against MHC class II exerted no inhibitory action on the lymphoid cell adhesion to FDC. As, at ultrastructural level, gold-labeled immune complexes can be found between FDC and lymphoid cells, we examined the effect on cell adhesion of the addition of immune complexes to the cell suspensions. It only impaired the lymphoid cell adhesion when complement components were present. IgM complexes were then more inhibitory than IgG complexes. When antibodies against Fc IgG receptors (CD16) were added, the adhesion was strongly reduced whereas antibodies to Fc IgE (CD23) receptors had no influence. The antibody DRC1, specifically recognizing an antigen on human FDC reduced the attachment of cells to FDC. This antigen thus seems to play a role in the intercellular contacts; this is the first function ascribed to this FDC specific antigen. [less ▲]

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See detailLocalization of interleukin 6 mRNA in human tonsils by in situ hybridization.
Bosseloir, A.; Hooghe-Peters, E. L.; Heinen, Ernst ULg et al

in European Journal of Immunology (1989), 19(12), 2379-81

We have investigated which areas produce interleukin 6 (IL 6) in human tonsils. This growth factor is required for the terminal differentiation of B lymphocytes into plasmocytes. Using 35S-labeled IL 6 ... [more ▼]

We have investigated which areas produce interleukin 6 (IL 6) in human tonsils. This growth factor is required for the terminal differentiation of B lymphocytes into plasmocytes. Using 35S-labeled IL 6 cDNA we demonstrated IL 6 gene expression over various areas of the tonsils, with consistent exception of the follicles, by in situ hybridization. It is, therefore, proposed that B cells are stimulated during their migration out of the follicles. [less ▲]

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See detailCytokines produced in lymph follicles.
Tsunoda, R.; Cormann, N.; Heinen, Ernst ULg et al

in Immunology Letters (1989), 22(2), 129-34

The events occurring inside lymph follicles during a germinal center reaction are poorly understood. Using B and T lymphoid cell populations prepared from human tonsillar lymph follicles, and enriched or ... [more ▼]

The events occurring inside lymph follicles during a germinal center reaction are poorly understood. Using B and T lymphoid cell populations prepared from human tonsillar lymph follicles, and enriched or not in macrophages or in follicular dendritic cells, we examined the production of cytokines by these cells in vitro. Interleukin 6 (IL-6) and tumor necrosis factor (TNF) were found in the supernatants of cultures stimulated with phytohemagglutinin or pokeweed mitogen. IL-1 beta was occasionally detected; its secretion apparently depends on the origin of the tonsils, the stimulation, and the cell populations. IFN-gamma and IL-2 were not produced in significant amounts by these lymph follicle cells. IL-4 was only found in very low concentrations in the supernatant of the different cell cultures. The cell populations containing follicular dendritic cells produced more IL-6 and TNF than the others, especially than those composed of only B and T cells. [less ▲]

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See detailEtude de la proliferation lymphocytaire humaine in vitro en reponse a l'anatoxine tetanique.
Lecart, Marie-Paule ULg; Cormann, N.; Heinen, Ernst ULg et al

in Revue Médicale de Liège (1989), 44(13-14), 455-63

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See detailFollicular dendritic cells act as accessory cells
Heinen, Ernst ULg; Cormann, N.; Lesage, F. et al

in Progress in Leukocyte Biology (1988), 7

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