References of "Heinen, Ernst"
     in
Bookmark and Share    
Peer Reviewed
See detailFollicular Dendritic Cells: Origin and Function
Heinen, Ernst ULg; Bosseloir, A.; Bouzahzah, F.

in Current Topics in Microbiology and Immunology (1995), 201

Detailed reference viewed: 13 (0 ULg)
Peer Reviewed
See detailLes cellules dendritiques folliculaires: phenotype, origine et fonctions.
Heinen, Ernst ULg

in Pathologie Biologie (1995), 43(10), 848-57

Follicular dendritic cells (FDC) are only located in lymph node follicles and particularly developed in the light zones of the germinal centers. FDC differ on important points from the classical dendritic ... [more ▼]

Follicular dendritic cells (FDC) are only located in lymph node follicles and particularly developed in the light zones of the germinal centers. FDC differ on important points from the classical dendritic cells presenting antigens to T cells. They are long-lived and derived from local mesenchymal cells. Their complex cytoplasmic extensions, covered with dendrites, are intermingled in a network which surrounds germinal B and T cells. On their surface, receptors fix immune complexes without endocytosis and adhesion molecules ensure close contacts to the lymphoid cells. FDC influence the migration, activation, proliferation, survival and differentiation of B cells into memory cells and probably act also on the germinal center T cells. [less ▲]

Detailed reference viewed: 82 (5 ULg)
See detailT cells in contact with follicular dendritic cells
Bouzahzah, F.; Suzuki, S.; Bosseloir, A. et al

in Follicular dendritic cells in normal and pathological conditions (1995)

Detailed reference viewed: 11 (0 ULg)
See detailB cells in contact with FDC
Bosseloir, A.; Bouzahzah, F.; Simar, L. et al

in Follicular dendritic cells in normal and pathological conditions (1995)

Detailed reference viewed: 19 (0 ULg)
See detailFDC : origin and function
Heinen, Ernst ULg; Bosseloir, A.; Bouzahzah, F. et al

in Current topics in Microbiology and Immunology (1995)

Detailed reference viewed: 10 (2 ULg)
See detailRadioresistance of follicular dendritic cells
Mancini, I.; Kinet-Denoël, C.; Brait, M. et al

in Follicular dendritic cells in normal and pathological conditions (1995)

Detailed reference viewed: 25 (1 ULg)
See detailThe follicular dendritic cells in normal and pathological conditions
Heinen, Ernst ULg

Book published by Springer (1995)

Detailed reference viewed: 9 (0 ULg)
Peer Reviewed
See detailMoabs Mas516 and 5b5, Two Fibroblast Markers, Recognize Human Follicular Dendritic Cells
Bosseloir, A.; Heinen, Ernst ULg; Defrance, T. et al

in Immunology Letters (1994), 42(1-2), 49-54

Follicular dendritic cells (FDC) are only located within follicles of secondary lymphoid tissues. The origin of this peculiar cell type is not clearly defined. To contribute to this study, we applied two ... [more ▼]

Follicular dendritic cells (FDC) are only located within follicles of secondary lymphoid tissues. The origin of this peculiar cell type is not clearly defined. To contribute to this study, we applied two monoclonal antibodies (MAS516 and 5B5) considered as specific for fibroblasts to tonsil cryosections and to isolated follicular dendritic cells. On the basis of an enzyme cocktail digestion of human tonsils and a fractionation procedure on albumin gradients, FDC can be prepared in the form of cell aggregates with associated lymphoid cells. MAS516 reacts with surface membrane molecules expressed by human fibroblasts, tissue macrophages and peripheral blood monocytes. With immunoperoxidase assays on tonsil cryosections connective tissue cells and macrophages are stained. Inside germinal centres, heavy labelling of the light zone was found. The MAS516 staining pattern is very similar to that of specific FDC markers DRC-1 or BU10. All isolated FDC reacted with MAS516 antibody. 5B5, considered as a typical fibroblast marker, reacts with human prolyl-4-hydroxylase which is an intracellular enzyme related to collagen biosynthesis. In cryosections, interfollicular and capsular areas showed 5B5 positive connective tissue fibroblasts. In germinal centres, some cells presenting features of FDC were 5B5 positive. After cell separation, 25%-50% of the isolated FDC were labelled with this antibody. This positivity of some FDC for 5B5 antibody may support the idea of their fibroblastic origin. The combination of observations realized in situ and after cell purification ensured an unequivocal recognition and identification of FDC.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]

Detailed reference viewed: 39 (0 ULg)
Peer Reviewed
See detailAgm-1470, a Potent Angiogenesis Inhibitor, Prevents the Entry of Normal but Not Transformed Endothelial Cells into the G1 Phase of the Cell Cycle
Antoine, Nadine ULg; Greimers, Roland ULg; De Roanne, C. et al

in Cancer Research (1994), 54(8), 2073-6

AGM-1470 is a potent angiogenesis inhibitor that is very effective in inhibiting endothelial cell proliferation in both in vitro and in vivo models and that prevents tumor growth in vivo. Although this ... [more ▼]

AGM-1470 is a potent angiogenesis inhibitor that is very effective in inhibiting endothelial cell proliferation in both in vitro and in vivo models and that prevents tumor growth in vivo. Although this molecule appears to be a most promising anticancer drug, its mechanism of action has not yet been elucidated. In this study, we examined the effects of AGM-1470 on the cell cycle of normal and transformed endothelial cells. We showed that AGM-1470, at picomolar concentrations, specifically inhibits the proliferation of both bovine aortic endothelial cells and human umbilical vein endothelial cells. AGM-1470 was ineffective in significantly inhibiting the proliferation of Ea.hy926 cells, a hybrid cell line obtained by the fusion of human umbilical vein endothelial cells with a human carcinoma cell line, or cEnd.1 cells, a polyoma middle T oncogene-transformed endothelioma cell line derived from mouse embryo. Using a double labeling technique with anti-Ki67 antibodies and propidium iodide, we demonstrated, with flow cytometry analysis, that AGM-1470 specifically prevents the entry of endothelial cells into the G1 phase of the cell cycle. We also showed that AGM-1470 was ineffective in inhibiting endothelial cell migration toward laminin or capillary-like tube formation inside a type I collagen matrix induced by phorbol esters. Our data strongly suggest that AGM-1470 is a molecule that specifically inhibits a cell cycle control pathway active in normal cells but which could be bypassed or altered in transformed cells. [less ▲]

Detailed reference viewed: 8 (2 ULg)
Peer Reviewed
See detailHuman follicular dendritic cells in vitro
Tsunoda, R.; Heinen, Ernst ULg; Imai, Y. et al

in Dendritic Cells (1994), 4

Detailed reference viewed: 10 (1 ULg)
Peer Reviewed
See detailExpression and function of DRC-1 antigen.
Bosseloir, A. L.; Antoine, Nadine ULg; Heinen, Ernst ULg et al

in Advances in Experimental Medicine and Biology (1994), 355

Detailed reference viewed: 9 (0 ULg)
Peer Reviewed
See detailFollicular dendritic cells: whose children?
Heinen, Ernst ULg; Bosseloir, A.

in Immunology Today (1994), 15(5), 201-4

At the recent Germinal Centre Conference in Spa*, over 200 immunologists gathered to study the fate of lymph follicles, B- and T-cell selection, cell migration, accessory cells, and pathological ... [more ▼]

At the recent Germinal Centre Conference in Spa*, over 200 immunologists gathered to study the fate of lymph follicles, B- and T-cell selection, cell migration, accessory cells, and pathological conditions such as lymphoproliferative diseases and AIDS. Here, Ernst Heinen and Alain Bosseloir report on this fascinating field. [less ▲]

Detailed reference viewed: 16 (1 ULg)
Peer Reviewed
See detailPlace of MALT in the immune defence system.
Heinen, Ernst ULg

in Advances in Experimental Medicine and Biology (1994), 355

Detailed reference viewed: 7 (0 ULg)
See detailIn Vivo Immunology: Regulatory Processes during Lymphopoiesis and Immunopoiesis
Heinen, Ernst ULg; Defresne, Marie-Paule ULg; Boniver, Jacques ULg et al

Book published by Plenum Press (1994)

Detailed reference viewed: 28 (6 ULg)
Peer Reviewed
See detailFollicular dendritic cells: isolation procedures, short and long term cultures.
Heinen, Ernst ULg; Tsunoda, R.; Marcoty, C. et al

in Advances in Experimental Medicine and Biology (1993), 329

Detailed reference viewed: 21 (1 ULg)
Peer Reviewed
See detailEmperipolesis of lymphoid cells by human follicular dendritic cells in vitro.
Tsunoda, R.; Nakayama, M.; Heinen, Ernst ULg et al

in Advances in Experimental Medicine and Biology (1993), 329

Detailed reference viewed: 12 (0 ULg)
Peer Reviewed
See detailFollicular Dendritic Cells Do Not Produce Tnf-Alpha nor its Receptor
Mancini, I.; Bosseloir, A.; Hooghe-Peters, E. et al

in Advances in Experimental Medicine and Biology (1993), 329

Detailed reference viewed: 13 (0 ULg)
Peer Reviewed
See detailUltrastructure of CD57+ cells isolated from human tonsils or blood.
Bouzahzah, F.; Heinen, Ernst ULg; Antoine, Nadine ULg et al

in European Journal of Morphology (1993), 31(1-2), 82-6

The presence of CD4+, CD57+ T cells in the germinal centers has been reported by several authors. The CD57 antigen is also expressed by natural killer (NK) cells. We purified CD57+ cells from human ... [more ▼]

The presence of CD4+, CD57+ T cells in the germinal centers has been reported by several authors. The CD57 antigen is also expressed by natural killer (NK) cells. We purified CD57+ cells from human tonsils and blood by microdissection, rosetting with sheep red blood cells and magnetic cell sorting (MACS) and examined the ultrastructural morphology of these cells. Clear differences were found in cell aspect: blood NK contained large granules which were not found in the tonsillar CD57+ cells. These latter appeared medium-sized and not fully activated. After immunolabeling, the tonsillar CD57+ cells were mainly found in the light zone of the germinal centers. [less ▲]

Detailed reference viewed: 10 (0 ULg)
Peer Reviewed
See detailDRC1 expression on normal and pathological lymphoid cells.
Antoine, Nadine ULg; Heinen, Ernst ULg; Marcoty, C. et al

in Advances in Experimental Medicine and Biology (1993), 329

Detailed reference viewed: 13 (0 ULg)
See detailImmunologie clinique
Heinen, Ernst ULg

Book published by De Boeck (1993)

Detailed reference viewed: 10 (3 ULg)