References of "Hayette, Marie-Pierre"
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See detailIn Vitro Activities of Strychnos Alkaloids and Extracts against Plasmodium Falciparum
Frederich, Michel ULg; Hayette, Marie-Pierre ULg; Tits, Monique ULg et al

in Antimicrobial Agents and Chemotherapy (1999), 43(9), 2328-31

The in vitro antimalarial activities of 46 alkaloids and extracts from Strychnos species were evaluated. Two types of quasidimeric alkaloids exhibit high and selective activities against Plasmodium ... [more ▼]

The in vitro antimalarial activities of 46 alkaloids and extracts from Strychnos species were evaluated. Two types of quasidimeric alkaloids exhibit high and selective activities against Plasmodium. Strychnopentamine and isostrychnopentamine were active against chloroquine-sensitive and -resistant strains (50% inhibitory concentration [IC(50)] approximately 0.15 microM), while dihydrousambarensine exhibited a 30-fold higher activity against the chloroquine-resistant strain (IC(50) = 0.03 microM) than it did against the chloroquine-sensitive strain. [less ▲]

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See detailUse of LIPA Mycobacteria (Innogenetics) to identify mycobacteria in a routine laboratory
Fauville-Dufaux, Maryse; Hayette, Marie-Pierre ULg; Vanfleteren, B. et al

Poster (1999, September)

The test consists in the PCR amplification of the DNA region coding for the 16S~23S rRNA space! of Mycobacterium species followed by the hybridization and the stringent wash of the amplified product with ... [more ▼]

The test consists in the PCR amplification of the DNA region coding for the 16S~23S rRNA space! of Mycobacterium species followed by the hybridization and the stringent wash of the amplified product with species specific probes immobilized on a strip. Thirteen different probes are placed on the strip enabling the specific identification of the most frequent mycobacterial strains isolated in clinical samples. One hundred twenty strains isolated from clinical specimens on Lowenstein-Jensen medium were included in the evaluation study. Lipa. identification of all the 120 strains was in agreement with identification obtained by other methods like classical bacteriological tests) Gen Probe hybridization tests, species specific PCRs. PeR-restriction fragment length polymorphism analysis ofthe fup65 gene (PRA) and, in some cases, DNA sequencing of an amplified fragment ofthe 168 rRNA gene. On the other hand, 35 early growing Bactec cultures from clinical specimens, taken at a GI as low as 9, were analysed by Lips Mycobacteria. 69 % ofthem could be identified on the aliquot taken when the GI was between 9 to 50. The remaining cultures could be identified on an aliquot ofBaetec mediwn taken one or 2 days later. In comparison to the other identification methods. Lipa Mycobacteria gives results by far faster than bacteriological tests and is easier to perfonn than PRA and DNA sequencing. It takes longer to perform than the Gen Probe hybridization test (lh30 PeR plus 3h30 hybridizationwashing procedure versus 2h for the Gen Probe test), but it allows, in the same procedure, identification of 9 different mycobacterial species though Gen Probe hyridiution only identifies 1 or maximum 2 species. Lipa Mycobacteria is also usable on early growing Bactec cultures. [less ▲]

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See detailComparative evaluation of Fungitest, Neosensitabs and broth microdilution method for yeasts susceptibility testing
Hayette, Marie-Pierre ULg; Bolland, Pascal; Seidel, Laurence ULg et al

Poster (1999, May)

The need of a simple and reliable method for routine yeasts susceptibility testing led us to evaluate two commercially available methods. We investigated the in vitro susceptibility of 67 clinical ... [more ▼]

The need of a simple and reliable method for routine yeasts susceptibility testing led us to evaluate two commercially available methods. We investigated the in vitro susceptibility of 67 clinical isolates (26 C. albicans, 32 C. glabrata, 4 C. krusei, 2 C. tropicalis, 2 C. kefyr and 1 S. cerevisiae) to 6 drugs flucytosine (FC), amphotericin B (A), fluconazole (FZ), itraconazole (IT), kétoconazole (K), miconazole (M), comparing two methods Fungitest (Sanofi Pasteur) and Neosensitabs (Rosco). A broth microdilution adaptation from the NCCLS-M27A procedure was used as reference method. Fungitests consist of individually packed 16 wells microplates containing 6 drugs at two critical concentrations in buffered medium. Reading was performed after 24 and 48h incubation. Neosensitabs is an agar diffusion method on Shadomy agar using antifungals tablets. Reading was performed after 24h. For all strains Neosensitabs was in concordance with NCCLS M27-A for FC (94%), A (98%), FZ (55%) I (53%) with p<0.05. Fungitest correlated with NCCLS method for all antifungals after 24 and 48h incubation time (p<0.05) with respectively 95/95% for FC, 100/100% for A, 80/76% for FZ, 81/55% for I and 93/75% for K, and 88/81 for M. Candida glabrata gave the poorest result with Neosensitabs with 28% concordance for FZand 39% for I; therefore the method can't be recommended for this species. Fungitest concordance observed was 55% for I after 48h. Our results suggest that Fungitest is appropriate for routine yeast susceptibility testing. However itraconazole testing has to be improved with this method. [less ▲]

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See detail10'-Hydroxyusambarensine, a New Antimalarial Bisindole Alkaloid from the Roots of Strychnos Usambarensis
Frederich, Michel ULg; Tits, Monique ULg; Hayette, Marie-Pierre ULg et al

in Journal of Natural Products (1999), 62(4), 619-21

Reinvestigation of Strychnos usambarensis Gilg resulted in the isolation of a tertiary phenolic bisindole alkaloid, 10'-hydroxyusambarensine (1), which was identified by detailed spectroscopic methods ... [more ▼]

Reinvestigation of Strychnos usambarensis Gilg resulted in the isolation of a tertiary phenolic bisindole alkaloid, 10'-hydroxyusambarensine (1), which was identified by detailed spectroscopic methods. Compound 1 was moderately active against two strains of Plasmodium falciparum in vitro. [less ▲]

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See detailA Comparative Study of the Antimycotic Activity of a Miconazole Hp-Beta Cyclodextrin Solution and a Surfactant Solution
Piel, Géraldine ULg; Hayette, Marie-Pierre ULg; Evrard, Brigitte ULg et al

in Journal de Pharmacie de Belgique (1999), 54(3, May-Jun), 87-8

The antimycotic activity of a new parenteral solution containing miconazole was compared to that of a marketed solution (Daktarin IV solution). This solution has been withdrawn from the belgian market ... [more ▼]

The antimycotic activity of a new parenteral solution containing miconazole was compared to that of a marketed solution (Daktarin IV solution). This solution has been withdrawn from the belgian market probably because of the toxic effects related to the presence of polyoxyl 35 castor oil. We propose a new formulation containing 10 mg of miconazole per ml (as the marketed solution), in combination with HP-beta cyclodextrin and lactic acid. The MIC of these two solutions were determined by broth microdilution method (following the NCCLS guidelines) against 15 yeasts and 16 filamentous fungi isolates. This study showed that MIC obtained with these two solutions are not significantly different. In vitro, the cyclodextrin solution has the same antimycotic activity as the Daktarin IV solution and can be proposed as a safe and effective parenteral solution to replace the previous surfactant solution. [less ▲]

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See detailA rapid immunochromatographic test (ICT) for serological diagnosis of tuberculosis
Hayette, Marie-Pierre ULg; Serpe, K.; Gonissen, Viviane et al

Poster (1998, November 28)

The lCT tuberculosis is an in vitro immunodiagnostic test for the detection of antibodies to Mycobacterium tuberculosis in serum or plasma. This test targets 5 recombinant M tuberculosis proteins ... [more ▼]

The lCT tuberculosis is an in vitro immunodiagnostic test for the detection of antibodies to Mycobacterium tuberculosis in serum or plasma. This test targets 5 recombinant M tuberculosis proteins, including the 38 and 85 KDa antigens. We evaluated this test on 65 sera from patients with and without TB living in Liege. Patients and methods: This test was performed on two groups : The TB group included 31 patients condidered to have an active tuberculosis : pulmonary (26 cases) or extrapulmonary (5 cases). All of them had at least one sample with positive culture and received anti-tuberculous treatment. The identification of TB strains was performed with Accuprobe M tuberculosis complexe (Gen-Probe). The second group (control group) included 34 patients with the following characteristics: BCG negative nonexposed healthy (19), BCG positive or exposed healthy (2), non-TB respiratory disease (10), atypical TB (3). The identification of the atypical mycobacteria was determined according to the biochemical characteristics or by molecular biology. Results. The sensitivity is 38.7%, the specificity is 94%. The positive predictive value is 85.7% and the negative predictive value is 62,7%. Two false lCT positive results were found in the control group: both patients had a past infection with TB. Conclusion: This test is very easy and rapid to perform, has a good specificity but lacks sensitivity in low prevalence countries. A positive lCT allows same day diagnosis of Mycobacterium tuberculosis infection. [less ▲]

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See detailDetection of bacteria in sterile body fluids
CHRISTIAENS, Geneviève ULg; HAYETTE, Marie-Pierre ULg; De Mol, Patrick ULg

Poster (1998, October)

Purpose of the study: Development of a polymerase chain reaction (PCR) method for the detection of any bacterial DNA in synovial, cerebrospinal, pleural, and peritoneal fluids. Methods: Most of bacterial ... [more ▼]

Purpose of the study: Development of a polymerase chain reaction (PCR) method for the detection of any bacterial DNA in synovial, cerebrospinal, pleural, and peritoneal fluids. Methods: Most of bacterial species possess highly conserved, multicopy 16S ribosomal RNA genes, which can be hybridized with a single set of oligonucleotide primers. Samples were processed by an extraction protocol using proteinase K, and subjected to PCR amplification using two universal bacterial primers: RW01 and DG74; then the PCR products were detected by ethidium bromide gel electrophoresis. Study: Synovial, cerebrospinal, pleural and peritoneal fluids, obtained from 32 patients were analyzed by Gram stain, culture and PCR. Results: 1. The limit of detection, determined by analyzing successive dilutions of Staphylococcus aureus and Escherichia coli cultures in sterile water, was: 1.105 cfu/100µl. 2. We obtained 9 positive samples by culture: - 7 synovial fluids: S. agalactiae, S. viridans, coagulase negative Staphylococcus (2), S. epidermidis (2), and S. aureus. - 2 pleural fluids: S. pyogenes and Enterobacter aerogenes. All were PCR positive. 3. We tested 23 culture negative samples. All were negative by PCR. Conclusion: PCR presents some interesting features: 1. Only small amount of sample is necessary (100µl). 2. The duration of the test is shorter than 8 hours. 3. PCR provides similar or eventually greater sensitivity than culture and an excellent specificity (no false-positive and no false-negative results actually observed). [less ▲]

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See detailCas d'un ulcère à Exophiala dermatitidis
Gérard, C.; Duchesne, Bernard ULg; Hayette, Marie-Pierre ULg et al

in Bulletin de la Société Belge d'Ophtalmologie (1998), 268

Les auteurs présentent un cas d'ulcère cornéen à Exophiala dermatitidis. Ce cas permet d'envisager la classification des mycoses cornéennes ainsi que leur fréquences. L'histoire clinique et l'aspect ... [more ▼]

Les auteurs présentent un cas d'ulcère cornéen à Exophiala dermatitidis. Ce cas permet d'envisager la classification des mycoses cornéennes ainsi que leur fréquences. L'histoire clinique et l'aspect biomicroscopique permettent de mettre en évidence les critères diagnostiques des infections mycotiques. L'intérêt et la techique des prélèvements seront discutés. l'analyse anatomopathologique après greffe de cornée confirme le diagnostic et nous rappelle la place essentielle de cet examen trop souvent négligé. L'attitude thérapeutique tant médicale que chirurgicale sera discutée. [less ▲]

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See detailTraveller's diarrhea due to microsporidia in a immunocompetent patient back from Guatemala
Hayette, Marie-Pierre ULg; Demonty, Jean ULg; Datry, Annick et al

Poster (1996, May)

Microsporidia are spore forming protozoan parasites who cause a variety of diseases among immunodeficiency patients. Only a few cases are reported among immunocompetent patients. We report a case of ... [more ▼]

Microsporidia are spore forming protozoan parasites who cause a variety of diseases among immunodeficiency patients. Only a few cases are reported among immunocompetent patients. We report a case of intestinal microsporidial infection in a 22-year-old student, coming back from Guatemala, after one month travel. Clinical aspects: The young boy was admitted to the hospital for napache with stiffness, backache and fever at 39°C. He reported nonbloody diarrhea 5 days before hospitalization, without nausea or vomiting. During his travel he also presented a few days of self-limited diarrhea, with watery stools without fever. At the end of the trip he had lost 10 kg. Before his travel he was vaccinated against A hepatitis, poliomyelitis, typhoid fever and he receved a malaria prophylaxis by Nivaquine. The clinical examination pointed out a discreet pain at the left iliac fossa, napache and fever. Diagnosis: The biology schows an inflammatory syndrome, hyperleucocytosis, and impairing of the hepatic tests with cholestasis. Routine cultures for bacterial pathogens were negative. Stool examination for parasites with use of direct examination or after diphasic concentration didn't reveal the presence of pathogens. The search of cryptosporidia was also negative. All the serologies even against the HIV for the search of a viral etiology were negative. By the use of a modified trichrome stain, some bright pink-red organism mesuring 1-2µ were detected by light microscopy in three consecutive stools. We concluded to the presence of protozoa of the phylum Microspora. Treatment: The patient receved first ciprofloxacine then albendazole, as specifically treatment. All the symptoms disappeared one month after hospitalization. The low charge of parasites didn't allow electron microscopy nor polymerase chain reaction for the determination of the species. [less ▲]

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See detailActualisation en mycologie
Hayette, Marie-Pierre ULg

Conference (1996, March 02)

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See detailIn vitro drug sensitivity and clinical aspects of Plasmodium falciparum malaria in African children
Carme, Bernard; Gay, Frederic; Ndounga, Mathieu et al

in Tropical Medicine and Parasitology : Official Organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft für Technische Zusammenarbeit (1995), 46(4), 270-274

In vitro Plasmodium falciparum drug sensitivity was investigated in 115 brazzavillians children, between 1 year and 10 years of age. On the basis of clinical aspects, four groups were constituted: Group 1 ... [more ▼]

In vitro Plasmodium falciparum drug sensitivity was investigated in 115 brazzavillians children, between 1 year and 10 years of age. On the basis of clinical aspects, four groups were constituted: Group 1: 39 asymptomatic school children, Group 2: 16 children with uncomplicated malaria, Group 3: 40 with severe but not pernicious malaria and Group 4: 20 with pernicious malaria. The drugs tested were chloroquine (CQ), quinine (QN) and mefloquine (MQ). The sensitivity level was assessed by a 48-hour in vitro maturation test involving the uptake of tritiated hypoxanthine, the initial blood level of parasite being > or = 0.1% in all cases. For QN and MQ, the median IC50 values showed no significant difference related to clinical status, age or parasitaemia levels. For CQ, the proportion of resistant strains and the 50 inhibitory concentration (IC50) values were greater in the cases of children hospitalised for malaria but there were no differences related to clinical severity of these hospitalised children nor, within each group, to the age or parasitaemia levels. The percentage of subjects with an IC50 value greater than the 90 percentile of the IC50 of the asymptomatic group, which we propose as the severity index related to chemoresistance, was 15% for uncomplicated malaria, 38% for severe but non-pernicious forms and 35% for pernicious malaria. The IC50 for QN was significantly higher in CQ-resistant strains and there was a positive correlation for CQ vs QN and for QN vs MQ. [less ▲]

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See detailChimiosensibilité in vivo et in vitro de Plasmodium falciparum à Brazzaville (Congo)
Chandenier, Jacques; Ndounga, Mathieu; Carme, Bernard et al

in Santé : Cahiers d'Etude et de Recherches Francophones (1995), 5(1), 25-29

Various projects were launched in 1993 to monitor the chemosensitivity of Plasmodium falciparum in Congo. Resistance of 34 strains in Brazzaville to chloroquine, quinine and mefloquine and of 35 to ... [more ▼]

Various projects were launched in 1993 to monitor the chemosensitivity of Plasmodium falciparum in Congo. Resistance of 34 strains in Brazzaville to chloroquine, quinine and mefloquine and of 35 to halofantrine was investigated in an in vitro survey using an isotopic micro test. The resistance rates were 61.8, 14.7, 3.0 and 0.0% respectively. Thus, the chemoresistance which first appeared in 1990 is confirmed and is stable in the population. This finding was further confirmed by a parallel in vitro analysis of sensitivity to chloroquine in Brazzaville. A chloroquine monitoring network is now being established throughout the country based on simplified WHO tests of 100 asymptomatic schoolchildren conducted every six months. The first results in 1993, from three Southern regions indicate that parasites are found in 20 to 60% of cases seven days after a standard 3 day treatment with 25 mg/kg, according to the region. The results of in vitro and in vivo tests are very variable. Indeed, the value of such results for these tests for national monitoring is questionable: a more reliable system of identifying true therapeutic failures would be better suited. [less ▲]

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See detailFive cases of infection due to Scedosporium apiospermum
Hayette, Marie-Pierre ULg; Peguet, C.; de Bièvre, Claude et al

Poster (1995)

We report five cases of infection due to Scedosporium apiospermum and his teleomorph Pseudallescheria boydii (Ascomycete), diagnosed in the University Amiens Hospital. The most important clinical ... [more ▼]

We report five cases of infection due to Scedosporium apiospermum and his teleomorph Pseudallescheria boydii (Ascomycete), diagnosed in the University Amiens Hospital. The most important clinical, epidemiological and diagnostic aspects a summurized in the next table. Nb year sex age job clinical statute site mycology (direct culture histology) Ampho B (S or R) TT 1 1992 F 31 secretary sinusitis sinus + S. apiospermum + S surg 2 1993 F 8 schoolgirl cystic fibrosis lung + P. boydii NR R physio 3 1993 M 66 pensioner corticoid cut/scut + S. apiospermum + R keto 4 1994 M 54 farmer corticoid sinus + P. boydii + S surg 5 1994 M 69 pensioner corticoid scut + S. apiospermum NR R surg ampho B: amphotericin B; tt: treatment; histo: histology; S or R: sensitive or resistant; surg: surgery; NR: non realized; physio: physiotherapy, cut/scut: cutaneous/subcutaneous. keto: ketoconazole. Epidemiology. The five cases concern french people living in Picardy (France). All have risk factors: corticotherapy, cystic fibrosis, sinusitis. The average age is 46 years with a sex-ratio 1,5. All the people are living in a rural area and one of them is farmer. The mode of contamination is probably the respiratory tract for the cases 1, 2 and 4. For the third one, the patient reported pricks during a walk in forest. The case 5 occured after forearm surgery. Clinical aspects. The young woman had a past of bacterial sinusal infection with posterior discharge since one year. Unsuccessful treatment With antibiotics and corticoids could explain the development of the fungus. The young girl affected by cystic fibrosis had no modification of the respiratory capacity and we concluded to a simple colonisation of the respiratory tract. For the case 5, the infection by Scedosporium didn't explain the death of the patient three days later. No case led to disseminated infection even for the patients under long-term corticotherapy. Mycology. The teleomorph, P. boydii was observed in only two cases with development of cleistothecia after several weeks of culture at room temperature. In all cases, direct examination showed numerous hyphae excepted the case 4, which showed numerous fungal cells with rare hyphae. Three of the five strains were "in vitro" resistant to amphotericine B and all were sensitive to ketoconazole. Treatment. The treatment were successful in all cases. Conclusion. These five cases of infection due to S. apiospermum are very similar to those described in the literature. Surgery seems to be the best treatment possible. All the strains exhibited low pathogenic power that proves the opportunistic character of this fungus. [less ▲]

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See detailIndice plasmodique et parasitémie à Plasmodium falciparum: valeur diagnostique et pronostique au Congo.
Carme, B.; Hayette, Marie-Pierre ULg; Mbitsi, A. et al

in Annales de la Société Belge de Médecine Tropicale (1972) (1995), 75

Parasitological data of various malarial studies performed in the Congo where Plasmodium falciparum malaria is holoendemic in rural and suburban zones between 1988 and 1991, were analyzed with the ... [more ▼]

Parasitological data of various malarial studies performed in the Congo where Plasmodium falciparum malaria is holoendemic in rural and suburban zones between 1988 and 1991, were analyzed with the intention of establishing diagnosis and prognosis value of Plasmodium falciparum parasitaemia in areas with high perennial transmission. In such an area Congolese school children (6-10 years old) had 88% P. falciparum index, this is the same percentage as that for children hospitalized with a pernicious attack. However, the parasite load is distributed differently; parasitaemia is greater than 6,000 asexual form for P. falciparum (af/Pf/µl) in only 4,6% of cases in the former group, versus 67% in the second group. A threshold of 10,000 af/Pf/µl above which the Plasmodium infection triggers a febrile attack in semi-immune children, is confirmed in school children in a rural context where the factor of taking antimalarial drugs within the preceding days is negligible: three out of four children with levels above this threshold are febrile versus 4,1% (7 out of 170) with lower blood parasite levels. Some adults where also asymptomatic carriers but much less frequently and with lower mean parasitaemia levels. The parasite load mirrors the clinical severity although this concept can be misleading as an individual prognostic criterion and foor hospital studies carried out in areas where multiple drug administration before hospitalization is common. For the studies recently performed in Brazzaville, the 5% threshold of parasitized red cells, the WHO severity criterion was never reached in asymptomatic subject or in cases of simple attack; it was reached in one out of two cases of pernicious attack. [less ▲]

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